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排序方式: 共有106条查询结果,搜索用时 15 毫秒
81.
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83.
Kawakami E Nishizono Y Miyata C Hirano T Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):119-123
The effects of hyaluronic acid, which comprises the cumulus intercellular matrix, on Ca(2+) influx, lactate dehydrogenase (LDH) activity, and cyclic AMP synthesis in canine sperm during capacitation was investigated. Ejaculated sperm were collected from 10 Beagle dogs and the sperm were incubated for 4 hr in Eagle's MEM containing 10 microg/ml of hyaluronic acid. The percentages of actively motile sperm, hyperactivated sperm (HA-sperm), acrosome-reacted sperm (AR-sperm), and sperm labeled with fluoresceinated Ca(2+) indicator (Ca(2+)-labeled sperm) were evaluated to assess Ca(2+) influx into the sperm. LDH activity and cAMP concentration were measured in homogenized sperm. The mean percentages of motile sperm, HA-sperm, and Ca(2+)-labeled sperm in the MEM containing hyaluronic acid were higher than in the control medium (P<0.05, 0.05, and 0.01, respectively), but there was no difference between the percentages of AR-sperm. Mean LDH activity and mean cAMP concentration were also significantly higher than the control values (P<0.05). The percentages of HA-sperm correlated with those of Ca(2+)-labeled sperm (r(2)=0.810). The results indicate that hyaluronic acid increases Ca(2+) influx, LDH activity, and cAMP synthesis in canine ejaculated sperm during capacitation in vitro. 相似文献
84.
Effect of feeding sweet‐potato condensed distillers solubles on intake and urinary excretion of minerals in Japanese Black steers
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Yuko Kamiya Misturu Kamiya Ikuo Hattori Yoshiro Hayashi Masayuki Funaba Tohru Matsui 《Animal Science Journal》2017,88(1):79-85
Four Japanese Black steers (16 months of age) were assigned to a 4 × 4 Latin square design to investigate the effect of graded levels of sweet‐potato condensed distillers solubles (SCDS) in their diets on intake and urinary excretion of minerals. The four diets consisted of 0%, 10%, 20% and 30% (dry matter (DM) basis) SCDS, with SCDS replacing commercial concentrate (CC). Intake of K, Cl, S, P and Mg increased linearly with increasing SCDS content. Urinary pH increased linearly with increasing dietary SCDS content. SCDS feeding increased urinary K concentrations (linear and quadratic effects). Urinary concentrations of Cl increased linearly with increasing SCDS content. In contrast, urinary concentrations of Mg decreased with increasing SCDS content. Feeding of SCDS did not apparently affect urinary NH3,P, Na or Ca concentrations. These results suggest that high SCDS feeding is not a risk for crystallization of minerals leading to the formation of magnesium‐phosphate type calculi: although SCDS contains large amounts of P and Mg, high SCDS feeding decreased the Mg concentration and did not affect the P concentration in urine. Additionally, high SCDS feeding had no apparent effects on plasma concentrations of Na, K, Cl, Ca or inorganic P. 相似文献
85.
Shuyang Ma Yongjun Tian Caihong Fu Haiqing Yu Jianchao Li Yang Liu Jiahua Cheng Rong Wan Yoshiro Watanabe 《Fish and Fisheries》2021,22(1):1-17
Climate‐induced nonlinearity in biological variability and non‐stationary relationships with physical drivers are crucial to understand responses of marine organisms to climate variability. These phenomena have raised concerns in the northeastern North Pacific, but are out of the spotlight in the northwestern North Pacific in spite of potential implications for this productive system under increased climate variability. Pelagic communities in the Kuroshio ecosystem have both ecological and economic importance. However, patterns of climate‐induced nonlinearity in pelagic communities are not well understood, and existence of non‐stationarity in their relationships with physical drivers remains obscure. Here, we compile large numbers of climatic, oceanic and biological long‐term time‐series data and employ diverse statistical techniques to reveal such climate‐induced nonlinearity and non‐stationarity. Results show that pelagic communities in the Tsushima and Pacific areas (major areas in the Kuroshio ecosystem) had regime shifts in the late 1990s and late 1980s, respectively. Winter sea surface temperatures in the Kuroshio Current path and in the eastern part of East China Sea, which are respectively affected by the Kuroshio Current and Siberian High, correlate with dominant variability patterns in their pelagic communities. Furthermore, non‐stationarity was identified with threshold years in the 1990s in the Tsushima area and in the 1980s in the Pacific area as a possible result of the declined variances in the Siberian High and Aleutian Low, respectively. Our findings provide insights on spatial differentiation of climate‐induced nonlinearity and non‐stationarity, which are valuable for the management of pelagic communities in the northwestern North Pacific under changing climatic conditions. 相似文献
86.
Naoya Ohtsuchi Tomohiko Kawamura Jun Hayakawa Hiroaki Kurogi Yoshiro Watanabe 《Fisheries Science》2018,84(2):211-225
Population dynamics of the kelp crab Pugettia quadridens, a potential predator of juvenile abalone, were investigated on the coast of Nagai, Sagami Bay, to examine its cryptic ontogenetic habitat shift. Monthly quantitative sampling over 2 years, which was carried out in 11 different subtidal habitats (< 0.1–8 m deep), revealed that P. quadridens changes ontogenetically according to its habitat: P. quadridens occurred in shallow lower subtidal (1–4 m) small red algal turfs (mainly Grateoloupia cornea) almost all year around, when the population was dominated by juveniles and stage I (immature) individuals; on the other hand, in the shallow upper subtidal (0.1–1 m) habitat (mainly Sargassum fusiforme beds near the low tidal mark), P. quadridens occurred only during winter to early spring, when the population was dominated by individuals of senior ontogenetic stages. These finding suggest that P. quadridens inhabit subtidal small red algal turfs during their early benthic phase, and then, the majority of larger individuals of stages II and III migrate to Sargassum beds. Our results suggest that the impact of P. quadridens predation on abalone stocks is limited in the present study site, primarily because the habitat of P. quadridens is segregated from that of juvenile abalone. 相似文献
87.
Konosuke OTOMARU Shun SAITO Karura ENDO Masayuki KOHIRUIMAKI Hiromichi OHTSUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):985-988
We investigated the effect of supplemental vitamin E on the peripheral blood leukocyte population in Japanese Black calves. Twenty-six calves kept at the same farm were studied. They were divided into two groups; thirteen calves received 300 IU/day of vitamin E orally from 1 to 3 months of age (VE group), and the other thirteen calves did not receive the vitamin E supplement (control group). The VE group showed a higher serum vitamin E concentration at 2 and 3 months of age compared with the control group (P<0.01). The numbers of CD3+ cells and CD4+ cells were higher in the VE group than in the control group, and the difference was statistically significant at 3 months of age (P<0.05). The numbers of CD21+ cells were higher in the VE group than in the control group, and the difference was statistically significant at 2 months of age (P<0.05). The numbers of CD335+ cells
tended to be higher in the VE group than in the control group. The numbers of CD8+ cells and CD14+ cells tended to be higher in the VE group than in the control group at 3 and 4 months of age. This study demonstrated that the supplementation of suckling Japanese Black calves with vitamin E might affect the numbers of some immune cell types in the peripheral blood. 相似文献
88.
Kiwamu HANAZONO Shinya FUKUMOTO Yoshio KAWAMURA Yoshifumi ENDO Tsuyoshi KADOSAWA Hidetomo IWANO Tsuyoshi UCHIDE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):1-6
Transitional cell carcinoma
(TCC), a urinary bladder tumor with high mortality, is encountered commonly in dogs.
Whereas overexpression of epidermal growth factor receptor (EGFR) is associated with
development of human urinary bladder cancer, information on EGFR expression in canine TCC
is lacking. In this study, EGFR protein and mRNA expression in canine normal bladder
(n=5), polypoid cystitis (n=5) and TCC (n=25) were examined by immunohistochemistry and
real-time polymerase chain reaction. EGFR protein expression was significantly higher in
TCC than that in normal healthy bladder (P<0.001) and polypoid
cystitis (P<0.005). High EGFR protein expression was significantly
(P<0.01) associated with TCC with a sensitivity of 72% and
specificity of 100%. Comparative analysis of protein and mRNA expression levels in TCC
showed significant positive correlation (r=0.88, P<0.05) between mRNA
and protein expression. These findings suggest that intense expression of EGFR protein
could be used as a marker to help canine TCC diagnosis. 相似文献
89.
Yoshiro ENDO Seiji HOBO Kenji KOROSUE Kenji OOTSUKA Akira KITAUCHI Risa KIKKAWA Yuichi HIDAKA Mitsuyoshi HAGIO Nao TSUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):507-509
The present study evaluated the effects of single-dose filgrastim on hematology in 16
healthy horses after long-distance transportation. Horses were assigned to receive
filgrastim (0.23 µg/kg, SC, once; G-CSF group; n=8) or saline (0.9% NaCl)
solution (0.3 ml, SC, once; control group; n=8) ≤ 1 hr before
transportation. Horses were transported 2,530 km using commercial vans over the course of
approximately 44 hr. Clinical examinations and hematologic analyses were performed on all
horses before and after transportation. Because the post-transportation white blood cell
counts and bacillary neutrophil to segmented neutrophil ratio were significantly higher in
the G-CSF group, filgrastim may have promoted the mobilization of neutrophils from marrow.
Filgrastim deserves a further study for efficacy in preventing horse shipping fever. 相似文献
90.
Shuyang Ma Caihong Fu Jianchao Li Peng Sun Yang Liu Zhenjiang Ye Yoshiro Watanabe Yongjun Tian 《Fish and Fisheries》2023,24(1):40-55
Non-stationary driver-response relationships are increasingly being recognized by scientists, underlining that a paradigm shift out of conventional stationary relationships is crucial. Japanese sardine (Sardinops melanostictus, Clupeidae) is a typical small pelagic fish in the northwestern Pacific with considerable fluctuations in productivity, bringing about great economic and ecological concerns. Numerous studies suggest that the population dynamics of Japanese sardine is an integrated process affected by multiple density-dependent, fishing and climatic drivers. However, little has hitherto been done to incorporate the non-stationary effects of multiple drivers, impeding progresses in understanding the population dynamics and in developing management strategies. In this study, we adopted variable coefficients generalized additive models to reveal the non-stationary effects of density dependence, fishing pressure and climatic conditions on the population dynamics of Japanese sardine. Results suggest that the dynamics of Japanese sardine from 1976 to 2018 could be divided into four periods: the 1980s when suitable climatic conditions from strong Siberian High pressure system sustained high abundance; the 1990s when negative density-dependent effects and degrading climatic conditions due to temperature increase led to population collapse; the 2000s when negative triple effects, particularly high fishing pressure, restricted the population increase; and the 2010s when favourable climatic conditions with re-strengthening Siberian High pressure system accompanied by low fishing pressure contributed to the population recovery. The study highlights that precise identifications of population status and climatic conditions are helpful to achieve good trade-offs between resource exploitation and protection and to facilitate ecosystem-based management for Japanese sardine fisheries. 相似文献