Cloning of the full-length coding sequence and expression analysis of Sox9b in guppy <Emphasis Type="Italic">(Poecilia reticulata)</Emphasis>     

Xue-Yan Shen  Jian-Zhou Cui  Qing-Li Gong  Yoshitaka Nagahama 《Fish physiology and biochemistry》2007,33(3):195-202

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Highly efficient induction of triploid Pacific abalone <Emphasis Type="Italic">Haliotis discus hannai</Emphasis> by caffeine treatment     

Sei-Ichi Okumura  Katsutoshi Arai  Yoshitaka Harigaya  Hirotaka Eguchi  Mizuho Sakai  Hiroaki Senbokuya  Suehiro Furukawa  Kunio Yamamori 《Fisheries Science》2007,73(2):237-243

ABSTRACT:   In order to develop a highly efficient method for mass production of triploid Pacific abalone Haliotis discus hannai , caffeine treatment that is safe and inexpensive was optimized. To suppress the first meiotic division, fertilized eggs were exposed to either a 10- or 15-mM caffeine solution for 24 min beginning at 12 min after fertilization. In most treated batches, the rates of cleaved eggs showed no significant difference from the control batches. However, in most treated batches, the rates of occurrence of normal larvae and the survival rates of the early juveniles were significantly lower than those of the controls. The triploid rates at 6 days to 11 months after settlement in all the treated batches were extremely high (91–100%). There was no significant difference in the mean triploid rates between 10- and 15-mM caffeine treatments. These results suggest that both treatment conditions were conducive to triploid abalone production. One live 2n/3n mosaic specimen was found in the treated batches. However, since the frequency of mosaic was extremely low, the mosaicism would probably not have an adverse effect on the stable production of triploid abalones.  相似文献   

Fruit and vegetable consumption and risk of colorectal cancer in Japan: The Miyagi Cohort Study     

Sato Y  Tsubono Y  Nakaya N  Ogawa K  Kurashima K  Kuriyama S  Hozawa A  Nishino Y  Shibuya D  Tsuji I 《Public health nutrition》2005,8(3):309-314

OBJECTIVE: Adequate fruit and vegetable intake has been suggested to protect against colorectal cancer. However, several recent prospective studies have reported no association. We therefore examined the association between fruit and vegetable intakes and the risk of colorectal cancer in a prospective cohort study in Japan. DESIGN: Between June and August 1990, 47 605 Japanese men and women completed a self-administered questionnaire, including a food-frequency questionnaire. We divided the subjects into quartiles based on their self-reported fruit and vegetable consumption. There were 165 colon cancer and 110 rectal cancer incidences identified during 7 years of follow-up, to the end of December 1997. We used Cox proportional hazards models to estimate the relative risk (RR) of developing colorectal cancer according to the level of fruit and vegetable consumption, applying adjustments for potential confounders. RESULTS: No statistically significant association was observed between fruit and vegetable consumption and the risk of colorectal cancer. The multivariate RR of colon cancer in the highest quartile of fruit and vegetable intake compared with the lowest was 1.13 (95% confidence interval (CI) 0.73-1.75), the RR for vegetables alone was 1.24 (95% CI 0.79-1.95) and that for fruit alone was 1.45 (95% CI 0.85-2.47). The corresponding multivariate RRs for rectal cancer were 1.12 (95% CI 0.67-1.89), 1.14 (95% CI 0.67-1.93) and 1.41 (95% CI 0.73-2.73). CONCLUSIONS: We found no association between the consumption of fruit and vegetables and the risk of colorectal cancer among the Japanese population.  相似文献   

Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar)   总被引：7，自引：0，他引：7  

Shimoji Y  Tamura Y  Nakamura Y  Nanda K  Nishidai S  Nishikawa Y  Ishihara N  Uenakai K  Ohigashi H 《Journal of agricultural and food chemistry》2002,50(22):6501-6503

Dihydroferulic acid (DFA) and dihydrosinapic acid (DSA) were isolated from Kurosu (unpolished rice vinegar) as the major constituents responsible for Kurosu's radical scavenging activity. The levels of antioxidative activity of DFA and DSA in DPPH radical scavenging were higher than those of their respective structurally related compounds, ferulic acid and sinapic acid. The concentrations of DFA and DSA were low in common rice vinegar (polished rice vinegar), suggesting that Kurosu is more advantageous than rice vinegars as an antioxidative food item. As the concentrations of DFA and DSA were low in unpolished rice, too, these acids are thought to be produced in Kurosu through the process of the fermentation from ferulic acid and sinapic acid, respectively.  相似文献   

Comparative observations on the growth changes of the histochemical property and collagen architecture of the Musculus pectoralis from Silky, layer-type and meat-type cockerels     

Ichiro OSHIMA  Hisao IWAMOTO  Shoji TABATA  Yoshitaka ONO  Akira ISHIBASHI  Nobuya SHIBA  Hideyuki MIYACHI  Takafumi GOTOH  Shotaro NISHIMURA 《Animal Science Journal》2007,78(6):619-630

Growth‐related changes in the histochemical properties and collagen architecture of the Musculus pectoralis were compared among Silky, layer‐type and meat‐type cockerels. Histochemical and immunohistochemical methods were used and collagen architecture was studied using scanning electron microscopy. The total amount of collagen present was also measured. The diameter of type IIB myofibers was similar or rather larger in the layer‐type birds compared with the meat‐type. The collagen content was generally low for 5–10 weeks across the breeds and then increased in the other breeds except for Silky. In the perimysium, the collagen bundles gradually increased in size and the density of the fibrils also increased during growth. At 30 weeks of age, the layer‐type birds showed compact collagen bundles while the meat‐type had loose bundles. The endomysial collagen network appeared relatively denser in the meat‐type chicks compared to the others at week 1. At 30 weeks of age, compact and felt‐like structure of endomysium was shown by Silky and layer‐type chickens, while the meat‐type showed a relatively loose arrangement of tissue in the endomysial collagen. From these results, it appears that the meat‐type chicken can produce a large M. pectoralis with many, relatively thinner myofibers and a relatively undeveloped form of intramuscular collagen structure.  相似文献   

Vibrational properties of heat-treated green wood   总被引：2，自引：0，他引：2  

Yoshitaka Kubojima  Takeshi Okano  Masamitsu Ohta 《Journal of Wood Science》2000,46(1):63-67

To investigate the influence of water on heat treatment, green wood was heat-treated. Sitka spruce (Picea sitchensis Carr.) with about 60% moisture content (MC) was used. Young's modulus and loss tangent were measured by the free-free flexural vibration test. The specimens were heated in nitrogen at 160°C for 0.5h. The results were as follows. (1) Recognizing that the effects of heat treatment are mild and that the same specimens cannot be used for both heat treatment and as controls, it was necessary to investigate the effects of the heat treatment based on the variations of properties in the whole of the test lumber. (2) Young's modulus increased and the loss tangent decreased due to heat treatment. When the vibrational properties were measured at various MCs, the MCs at the maximum value of Young's modulus and the minimum value of the loss tangent were lower in heat-treated specimens than in controls. The effects of heat treatment in green wood were similar to those in air-dried wood. (3) The loss tangents of heat-treated specimens were smaller than those of controls at about 0% MC but were larger than those of controls at about 10% MC. We thought that this resulted from the decreased MC at the minimum loss tangent after the heat treatment mentioned above. (4) The properties measured at several MCs were more useful than those at only one moisture content for investigating the effects of heat treatment.This study was presented in part at the 46th annual meeting of the Japan Wood Research Society, Kumamoto, April 3–5, 1996; and at the 47th annual meeting of the Japan Wood Research Society, Kochi, April 3–5, 1997  相似文献   

Photosynthetic capacity and nitrogen partitioning in foliage of the evergreen shrub Daphniphyllum humile along a natural light gradient     

Katahata S  Naramoto M  Kakubari Y  Mukai Y 《Tree physiology》2007,27(2):199-208

We examined the effects of leaf age and mutual shading on the morphology, photosynthetic properties and nitrogen (N) allocation of foliage of an evergreen understory shrub, Daphniphyllum humile Maxim, growing along a natural light gradient in a deciduous Fagus crenata-dominated forest in Japan. Seedlings in high-light environments were subject to greater mutual shading and 1-year-old foliage survival was lower than in seedlings in low-light environments, indicating that the survival rates of foliage were related to the degree of mutual shading. Although specific leaf area (SLA) in current- and 1-year-old foliage was curvilinearly related to daily photosynthetic photon flux (PPF), SLA was unaffected by leaf age, indicating that foliage in D. humile may not acclimate morphologically to annual changes in light caused by mutual shading. Light-saturated net photosynthetic rates (Pmax) were correlated with daily PPF in current-year foliage. In addition, a strong, positive relationship was found between nitrogen concentration per unit leaf area and Pmax. In contrast, the relationship among PPF, N and photosynthetic parameters in 1-year old foliage was weak because of the strong remobilization of N from older leaves to current-year foliage in plants growing in high light. However, the relationship between daily PPF and both photosynthetic N-use efficiency and the ratio of maximum electron transport rate to maximum carboxylation rate did not differ between current-year and 1-year-old foliage, suggesting that these responses help maintain a high photosynthetic efficiency even in older foliage. We conclude that D. humile maximizes whole-plant carbon gain by maintaining a balance among photosynthetic functions across wide ranges of leaf ages and light environments.  相似文献   

云南稻-鸭共生模式效益的研究及综合评价（三）     

戴志明  杨华松张 曦 周晓智韩自鸿 余杨钱国平  张树清万田正治 中西良孝 魏红江 《勤云标准版测试》2004,(4)

通过不同放鸭只数的试验,研究了在中高产田单位面积放鸭的数量与产量构成因素和病虫草害发生的关系,分析了产量构成,病虫草害发生的情况,为稻一鸭共生模式技术的推广提供理论依据。  相似文献   

One-dimensional fast migration of vacancy clusters in metals     

Matsukawa Y  Zinkle SJ 《Science (New York, N.Y.)》2007,318(5852):959-962

The migration of point defects, for example, crystal lattice vacancies and self-interstitial atoms (SIAs), typically occurs through three-dimensional random walk in crystalline solids. However, when vacancies and SIAs agglomerate to form planar clusters, the migration mode may change. We observed nanometer-sized clusters of vacancies exhibiting one-dimensional (1D) fast migration. The 1D migration transported a vacancy cluster containing several hundred vacancies with a mobility higher than that of a single vacancy random walk and a mobility comparable to a single SIA random walk. Moreover, we found that the 1D migration may be a key physical mechanism for self-organization of nanometer-sized sessile vacancy cluster (stacking fault tetrahedron) arrays. Harnessing this 1D migration mode may enable new control of defect microstructures such as effective defect removal and introduction of ordered nanostructures in materials, including semiconductors.  相似文献   

Diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in Japan     

Yuichi SAEKI  Naoto AIMI  Shoko TSUKAMOTO  Takeo YAMAKAWA  Yoshitaka NAGATOMO  Shoichiro AKAO 《Soil Science and Plant Nutrition》2006,52(4):418-426

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