Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L. )     

Nakatani N  Kayano S  Kikuzaki H  Sumino K  Katagiri K  Mitani T 《Journal of agricultural and food chemistry》2000,48(11):5512-5516

Neochlorogenic acid (3-CQA) and cryptochlorogenic acid (4-CQA), isolated from prune (Prunus domestica L.), were identified by NMR and MS analyses. In addition, the quantity of chlorogenic acid isomers in prune were measured by HPLC. These isomers, 3-CQA, 4-CQA, and chlorogenic acid (5-CQA), were contained in the ratio 78.7:18. 4:3.9, respectively. 4-CQA was identified and quantified in prune for the first time, and relatively high amounts of this isomer were characteristic. Antioxidative activities of the chlorogenic acid isomers, such as scavenging activity on superoxide anion radicals and inhibitory effect against oxidation of methyl linoleate, were also evaluated. Each isomer showed antioxidative activities which were almost the same.  相似文献   

Progression of Subaortic Stenosis Detected by Continuous Wave Doppler Echocardiography in a Dog     

Tomohiro Nakayama  Yoshito Wakao  Ryokichi Ishikawa  Mitsugi Takahashi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1996,10(2):97-98

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Antioxidative and antiradical activities of <Emphasis Type="Italic">Eucalyptus camaldulensis</Emphasis> leaf oils from Thailand     

Pornpun Siramon  Yoshito Ohtani 《Journal of Wood Science》2007,53(6):498-504

The leaf essential oils (six samples) from three clones of Eucalyptus camaldulensis Dehnh. were characterized by gas chromatography-mass spectrometry. Radical scavenging and antioxidant properties were investigated by the 1,1-diphenyl-2-picylhydrazyl (DPPH) assay and the β-carotene bleaching test. It was found that the whole essential oil and its fractions had significant antioxidant effects when they were tested by each method. In the DPPH assay, the E. camaldulensis leaf oils showed IC₅₀ inhibitory concentrations in the range of 1.75–12.62 mg/ml. In the β-carotene bleaching test, the IC₅₀ valued were in the range of 14.30–118.55 μg/ml. Part of this report was presented at the 18th meeting of the Chugoku-Shikoku Branch of the Japan Wood Research Society, November 2006  相似文献   

Regulation of trophoblast-specific factors by GATA2 and GATA3 in bovine trophoblast CT-1 cells     

Bai H  Sakurai T  Someya Y  Konno T  Ideta A  Aoyagi Y  Imakawa K 《The Journal of reproduction and development》2011,57(4):518-525

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Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study     

Nakano H  Kameo Y  Andoh K  Ohno Y  Mochizuki M  Maeda K 《Veterinary microbiology》2009,138(1-2):179-183

Antimicrobial therapy is a useful tool to control bovine mastitis caused by Staphylococcus aureus, as consequence an increase in staphylococci resistant cases has been registered. Alternative strategies are desirable and bacteriocins represent attractive control agents to prevent bovine mastitis. The aim of this work was to evaluate the activity of five bacteriocins synthesized by Bacillus thuringiensis against S. aureus isolates associated to bovine mastitis. Fifty S. aureus isolates were recovered from milk composite samples of 26 Holstein lactating cows from one herd during September 2007 to February 2008 in México and susceptibility of those isolates to 12 antibiotics and 5 bacteriocins from B. thuringiensis was evaluated. S. aureus isolates were mainly resistant to penicillin (92%), dicloxacillin (86%), ampicillin (74%) and erythromycin (74%); whereas susceptibility to gentamicin, trimethoprim and tetracycline was detected at, respectively, 92%, 88%, and 72%. All S. aureus isolates showed susceptibility to the five bacteriocins synthesized by B. thuringiensis, mainly to morricin 269 and kurstacin 287 followed by kenyacin 404, entomocin 420 and tolworthcin 524. Our results showed that S. aureus isolates had differences in the antimicrobial resistance patterns and were susceptible to bacteriocins produced by B. thuringiensis, which could be useful as an alternative method to control bovine mastitis.  相似文献   

Effect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos   总被引：1，自引：0，他引：1  

Atabay EC  Katagiri S  Nagano M  Takahashi Y 《The Japanese journal of veterinary research》2003,50(4):185-194

Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 microm) cells that made up 56% of total cells was at the G0/G1 phase. NT embryos were constructed by electric fusion with the medium-sized serum-starved cumulus cells and bovine oocytes of 3 different preparations: enucleated oocytes treated with calcium ionophore A 23187 for 5 min and cycloheximide for 5 hr (A 23187/CHX), those treated with ethanol for 7 min and cycloheximide for 2 hr(ethanol/CHX) and those without treatment. In Exp. 2 and 3, developmental competence of NT embryos constructed with A 23187/CHX- and ethanol/CHX-treated oocytes was compared to that of NT embryos constructed with non-treated oocytes, respectively. Further, nuclear behavior in 3 different NT embryos was examined in Exp. 4. Within 1 hr after fusion, majority of the NT embryos constructed with non-treated oocytes showed condensed chromosome. Three hours after fusion, about 50% of NT embryos constructed with non-treated or ethanol/CHX-treated oocytes showed a single pronucleus-like structure. NT embryos constructed with ethanol/CHX-treated oocytes showed similar rates of fusion, cleavage and blastocyst formation to those of the non-treated oocytes. In contrast, NT embryos constructed with A 23187/CHX-treated oocytes did not show any pronucleus-like structure and showed lower cleavage rate and no development to blastocysts. The results indicate that ethanol/CHX-treated oocytes could support development of somatic cell NT embryos to the blastocyst stage at a similar rate to that of non-treated oocytes.  相似文献   

Relationship among growth, steroid production and immunolocalization of transforming growth factor-beta 1 in the normally developing mouse follicles cultured in vitro     

Bishonga C  Takahashi Y  Katagiri S  Nagano M  Ishikawa A 《The Japanese journal of veterinary research》2001,48(4):169-176

This study examined the relationship among growth, steroid production and transforming growth factor-beta 1 (TGF-beta 1) immunolocalization in the mouse follicles cultured in vitro to evaluate the hypothesis that normally developing follicles should express TGF-beta 1 in the granulosa cells around the time of antrum formation. Preantral follicles with 151-175 microns (large category) and 125-150 microns (small category) of initial diameters were used as models for normal and retarded follicles, respectively. Growth rate and timing of antrum formation in both categories were comparable to those of in-vivo grown follicles. At the time of antrum formation, follicular diameters were similar between the two follicle categories; however, antral follicles from the large category showed larger number of granulosa cells, higher estradiol production and proportion of follicles with TGF-beta 1 positive granulosa cells. Two days after antrum formation, there were no differences in the number of granulosa cells and the proportions of follicles with TGF-beta 1 positive granulosa or theca cells between the two categories. Temporal association in large follicles between the increase in estradiol production and proportion of follicles with TGF-beta 1 positive granulosa cells at the time of antrum formation supports our hypothesis. Furthermore, this study demonstrated the usefulness of the follicle culture system in the investigations of follicular physiology.  相似文献   

In vitro culture of mouse preantral follicles using membrane inserts and developmental competence of in vitro ovulated oocytes   总被引：3，自引：0，他引：3  

Adam AA  Takahashi Y  Katagiri S  Nagano M 《The Journal of reproduction and development》2004,50(5):579-586

To develop a reliable follicle culture system, mouse preantral follicles 150-200 microm in diameter were cultured individually for 5 or 6 days in membrane inserts or in droplets, and then induced to ovulate with hCG (Experiment 1). The nuclear maturation and developmental competence of the oocytes that ovulated from the follicles cultured in inserts were determined (Experiment 2). There was no significant difference between the two culture systems in the survival rate (83 and 77%). However, follicles cultured in inserts showed a higher ovulation rate (63%) than those cultured in droplets (39%, P<0.05). About 80% of the oocytes that ovulated from the follicles cultured in inserts were at the metaphase II stage. After in vitro fertilization, 75 and 48% of in vitro ovulated oocytes cleaved and developed into blastocysts, respectively. These results demonstrate that the insert culture system is superior to the droplet culture system in terms of follicular growth and ovulation, and can be used to investigate the growth and ovulation of follicles in vitro.  相似文献   

Three substrains with different hemagglutination patterns isolated from a Japanese lentogenic strain of Newcastle disease virus     

S Yachida  Y Iritani  K Katagiri 《Avian diseases》1979,23(4):979-982

Three substrains were cloned from chick kidney cell monolayers infected with the Ishii strain of lentogenic Newcastle disease virus isolated in Japan. These substrains, which differed in plaque morphology, were distinguished as having no +24R+ and -24R- patterns in the hemagglutination (HA)-elution test.  相似文献   

Effect of medium change on the development of in vitro matured and fertilized bovine oocytes cultured in medium containing amino acids   总被引：3，自引：0，他引：3  

Ikeda K  Takahashi Y  Katagiri S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):121-123

Bovine in vitro matured and fertilized oocytes were cultured for 153 hr in groups of 3 or 30 in 30 microl of modified synthetic oviduct fluid medium supplemented with amino acids. The concentration of ammonium in culture medium at 153 hr of culture was significantly decreased by medium change at 72 hr of culture. However, regardless of embryo density, medium change had no beneficial or detrimental effect on the development of bovine embryos. Increase in the development to blastocysts and production of ammonium were observed when embryos were cultured in groups of 30. These results indicated that the ammonium concentration detected in this culture system has a negligible effect on the development of bovine embryos to blastocysts.  相似文献