Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)     

Walid El-Rodeny  Mitsuhiro Kimura  Hideki Hirakawa  Attia Sabah  Kenta Shirasawa  Shusei Sato  Satoshi Tabata  Shigemi Sasamoto  Akiko Watanabe  Kumiko Kawashima  Midori Kato  Tsuyuko Wada  Hisano Tsuruoka  Chika Takahashi  Chiharu Minami  Keiko Nanri  Shinobu Nakayama  Mitsuyo Kohara  Manabu Yamada  Yoshie Kishida  Tsunakazu Fujishiro  Sachiko Isobe 《Breeding Science》2014,64(3):252-263

To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a ‘Nubaria 2’ × ‘Misr 3’ F₂ mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F₂ plants were divided into three subpopulations according to the original F₁ plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated ‘Nubaria 2’ × ‘Misr 3’ map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.  相似文献   

Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis)     

Shinichi WATANABE  Megumi MIURA  Hiromi MORITA  Moeka NISHI  Shin-ichi YOKOTA  Shosaku HATTORI  Hiromichi MATSUMOTO  Emiko FUKUI  Ken Takeshi KUSAKABE  Masanori OCHI  Naomi NAKAGATA  Yasuo KISO  Chieko KAI  Midori YOSHIZAWA 《The Journal of reproduction and development》2021,67(4):265

Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. The unilateral ovary was excised after the administration of human chorionic gonadotropin (hCG), to induce ovulation, following which the larger developed follicular oocytes were collected. Follicular oocytes were prepared for ICSI using sperm from the epididymal tail of a unilateral testis extracted from a mature male. The embryos were continuously incubated in CMRL 1066 medium supplemented with 10% (v/v) fetal bovine serum. Embryo culture was performed with cumulus cells. Two experiments of ICSI carried out with three females resulted in 14 mature oocytes from the 49 cumulus-oocyte complexes collected and five embryos, three of which developed into blastocysts. These blastocysts were vitrified, thawed, and transferred to recipient monkeys, but no pregnancies resulted. In conclusion, the present study is the first to successfully produce ICSI-derived blastocysts from MII oocytes obtained by means of hormone administration (a combination of eCG+inhibin antiserum and hCG) and in vitro maturation in immature squirrel monkeys.  相似文献   

Reaction behavior of wood in an ionic liquid, 1-ethyl-3-methylimidazolium chloride   总被引：2，自引：0，他引：2  

Hisashi Miyafuji  Kenji Miyata  Shiro Saka  Fumio Ueda  Midori Mori 《Journal of Wood Science》2009,55(3):215-219

Reaction of Japanese beech (Fagus crenata) in an ionic liquid, 1-ethyl-3-methylimidazolium chloride ([C2mim][Cl]), which can dissolve cellulose, was investigated. Although both lignin and polysaccharides such as cellulose and hemicelluloses can be liquefied at a treatment temperature of around 100°C, the liquefaction of polysaccharides mainly occurs at the beginning of the treatment with [C2mim][Cl]. Cellulose crystallinity in the wood was gradually broken down as the treatment continued. The solubilized polymers were depolymerized to low molecular weight compounds. The results indicate that [C2mim][Cl] is an effective solvent and reagent for the liquefaction of wood components and subsequent depolymerization of them. Part of this report was presented at the 58th Annual Meeting of the Japan Wood Research Society, Tsukuba, April 2008  相似文献   

Impacts of the nonlinear relationship between abundance and its index in a tuned virtual population analysis     

Midori Hashimoto  Hiroshi Okamura  Momoko Ichinokawa  Kazuhiko Hiramatsu  Takashi Yamakawa 《Fisheries Science》2018,84(2):335-347

The abundance index used in a tuned virtual population analysis (VPA) is usually assumed to be proportional to actual abundance. However, the actual abundance and abundance index do not always have a linear relationship. Such nonlinearity can cause biases in abundance estimates as well as retrospective biases arising from systematic differences in abundance estimates when more data are successively added. Severe retrospective biases can damage the reliability of stock assessments. In this study, we use an approach to estimate an additional parameter that controls the nonlinearity in a tuned VPA. A performance test using simulated data revealed that the tuned VPA was able to accurately estimate the nonlinearity parameter and thus yielded less biased abundance estimates and smaller retrospective biases. We also found that estimating the nonlinearity parameters was effective even under other model misspecification scenarios, such as disregarding historical increases in catchability and time-varying natural mortality. Moreover, we applied this approach to some Japanese fish stocks and evaluated its validity. We found that estimating the nonlinearity parameters in the tuned VPA enhances the reliability of fisheries stock assessments.  相似文献   

Bite Wounds Caused by a Wild Boar: A Case Report     

Hiroki Nagasawa  Kazuhiko Omori  Hiroyuki Maeda  Ikuto Takeuchi  Suguru Kato  Takashi Iso  Kei Jitsuiki  Toshihiko Yoshizawa  Kouhei Ishikawa  Hiromichi Ohsaka  Youichi Yanagawa 《The Forestry Chronicle》2017,28(4):313-317

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日本对虾杆状核病毒性传染病诊断技术     

Kazuo Momoyama  李常勇 《河北渔业》1997,(2):5-10

本文提出了分别用暗视野显微技术和透射电子显微技术快速确诊日本对虾杆状核病毒性传染病的方法。在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm;胃表层上皮被病毒感染的细胞核明显地呈白色均质小体,直径10～15μm,球形或椭圆形,坏死细胞被囊化为棕色小块(约20～50μm)。病虾血淋巴和胃经负染制样在透射电子显微镜下观察病毒颗粒大小为400×150nm;核衣壳大小为390×85nm。  相似文献   

Identification of a second dynamic state during stick-slip motion     

Yoshizawa H  McGuiggan P  Israelachvili J 《Science (New York, N.Y.)》1993,259(5099):1305-1308

Stick-slip, or interrupted, motion rather than smooth uninterrupted motion occurs in many different phenomena such as friction, fluid flow, material fracture and wear, sound generation, and sensory "texture." During stick-slip, a system is believed to undergo transitions between a static (solid-like) state and a kinetic (liquid-like) state. The stick-slip motion between various types of pretreated surfaces was measured, and a second, much more kinetic state that exhibits ultra-low friction was found. Transitions to and from this super-kinetic state also give rise to stick-slip motion but are fundamentally different from conventional static-kinetic transitions. The results here suggest practical conditions for the control of unwanted stick-slip and the attainment of ultra-low friction.  相似文献   

The first record of sucking louse, Neohaematopinus callosciuri, infesting pallas squirrels in Japan     

Shinozaki Y  Yoshizawa K  Murata K  Shiibashi T  Kimura J  Maruyama S  Hayama Y  Yoshida H  Nogami S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):333-335

A species of sucking louse, Neohaematopinus callosciuri, was found for the first time in Japan. The species was found on an invasive species of squirrel, Pallas squirrel, Callosciurus erythraeus, in the Kamakura district, Kanagawa Prefecture, Japan. A total of 52 lice were obtained from 22 of 104 squirrels captured. The lice were about three times more prevalent in male squirrels than in females and were detected most frequently in the winter. As N. callosciuri has never been reported on wild animals in Japan, this species probably was introduced into Japan along with their host, Pallas squirrels.  相似文献   

Hypoglycemia associated with disseminated metastatic tonsillar squamous cell carcinoma producing insulin‐like growth factor‐I in a Pomeranian dog     

Kazushi Azuma  Makoto Asakawa  Toshiaki Konno  Shoko Fukuda  Midori Goto Asakawa 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(3):429-434

A 13‐year‐old spayed female Pomeranian dog was presented for persistent, severe hypoglycemia (37 mg/dL; reference interval [RI] 75‐128 mg/dL). Progressive nonregenerative anemia (hematocrit 23.3%‐15.9%; RI 37.0%‐55.0%) and severe thrombocytopenia (36 000/µL; RI 200‐500 000/µL) were also noted. The serum insulin concentration was low (0.24 ng/mL; RI 0.302‐1.277 ng/mL). Computed tomography revealed multiple splenic nodules (1‐6 mm in diameter) and several hepatic nodules (7.6, 12 mm in diameter). Ultrasound‐guided fine‐needle aspiration of the splenic and hepatic nodules revealed low numbers of epithelial cells with mild cellular atypia, suggestive of a metastatic epithelial tumor, but the primary site was unknown at that time. On careful oral examination under general anesthesia, an enlarged right tonsil was noted grossly, and histopathologic examination of the tonsil diagnosed squamous cell carcinoma. Bone marrow aspirates and biopsies of the splenic and hepatic nodules were performed; all samples were diagnosed as metastatic carcinoma on histopathologic examination. No nodules were present in the pancreas, despite careful palpation during exploratory laparotomy. On immunohistochemistry, the neoplastic cells were positive for cytokeratin AE1/3 and insulin‐like growth factor (IGF)‐I but were negative for chromogranin A, PGP9.5, insulin, and inconclusive for IGF‐II. This is the first report of a primary IGF‐I‐producing squamous cell carcinoma in the tonsil of a dog with metastases to bone marrow, liver, and spleen, resulting in hypoglycemia.  相似文献   

What is your diagnosis? Mandibular mass in a cat          下载免费PDF全文

Ashleigh W. Newman  Midori G. Asakawa  Tracy Stokol 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(3):501-502

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