Brief communication: replication of feline infectious peritonitis virus in organ cultures of feline tissue.     

Y Hoshino  F W Scott 《The Cornell veterinarian》1978,68(3):411-417

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Serological identification of animal species of meat using species-specific anti-serum albumin antibodies obtained by immunoadsorbent chromatography.     

T Kamiyama  Y Katsube  K Imaizumi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1978,40(6):663-669

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The in vivo sensitivity of ATPases to DDT,DDE, and physical immobilization in American cockroaches     

L.K. Cutkomp  D. Desaiah  E.Y. Cheng  E.V. Vea  R.B. Koch 《Pesticide biochemistry and physiology》1976,6(3):203-208

American cockroaches injected with sublethal doses of DDT (0.75 μg/roach) at 5-day intervals showed a 40% reduction in oligomycin-sensitive Mg²⁺ATPase from muscle homogenates, and a 23% reduction of Na⁺-K⁺ATPase from nerve cords. Thus, the maximum effect measured occurred with the same enzyme and tissue as determined from in vitro studies. The metabolite, DDE, used at 15 μg per roach, gave no significant change in activity of the ATPase system following injection. In contrast, high single doses of DDT (7.5 μg/roach) and 100 μg DDE and dicofol per roach caused over 30% increase in oligomycin-sensitive Mg²⁺ATPase of muscle and a 10–15% increase in Na⁺-K⁺ATPase of nerve cords measured 24 and 48 hr later. While a similar response was observed for Mg²⁺ATPase activities in cockroaches that were immobilized, the increase in enzyme activities were much greater than that caused by the pesticides.  相似文献   

Differential tolerance of peas to prometryne and terbutryn     

Y. Eshel  M. Kovacs  B. Rubin 《Pesticide biochemistry and physiology》1975,5(3):295-302

The phytotoxicity of 2,4-bis(isopropyl)-6-(methylthio)-s-triazine (prometryne) and 2-(tert-butylamino)-4-(ethylamino)-6-(methylthio)-s-triazine (terbutryn) to peas (Pisum sativum L. var. Perfection 3040) was studied. No differences were found when the herbicides were applied to the roots of intact plants in nutrient solution or directly to leaf discs. However, prometryne was much more toxic when uptake was from soil. Absorption and translocation of ¹⁴C-labeled prometryne and terbutryn showed that the majority of terbutryn accumulated in the roots, whereas prometryne was uniformly distributed between the roots and the shoot. Thin layer chromatography of extracts from prometryne-treated peas showed that only 20% of the absorbed compound was metabolized to produce one breakdown product. Extracts of terbutryn-treated plants contained three different metabolites. After 120 hr of exposure to terbutryn, about half of the absorbed herbicide was metabolized. The results show that the main factors responsible for the differential toxicity of the herbicides to peas were availability from the soil, translocation pattern and initial detoxication.  相似文献   

Effect of infections with barley yellow dwarf virus and Fusarium spp. on assimilation of 14CO2 by flag leaves and translocation of photosynthates in wheat     

Liu  Y.  Buchenauer  H. 《植物病害和植物保护杂志》2005,112(6):529-543

Journal of Plant Diseases and Protection - The effects of infections of Barley yellow dwarf virus (BYDV), Fusarium culmorum and F. graminearum alone as well as double infections of BYDV and...  相似文献   

DNA Immunization Against Very Virulent Infectious Bursal Disease Virus with VP2‐4‐3 Gene and Chicken IL‐6 Gene     

J. H. Sun  Y. X. Yan  J. Jiang  P. Lu 《Zoonoses and public health》2005,52(1):1-7

The present study was to investigate the feasibility and efficiency of the DNA vaccine to protect chickens against very virulent infectious bursal disease virus (vvIBDV) infection. A plasmid DNA carrying VP2‐4‐3 genes of vvIBDV SH95 and a plasmid DNA carrying chicken interleukin‐6 (ChIL‐6) genes were constructed and designated as pALTER‐MAX‐VP2‐4‐3 and pALTER‐MAX‐ChIL‐6 respectively. Several DNA vaccination experiments were performed: 1‐week‐old chickens were intramuscularly injected with only plasmid pcDNA3‐VP2, pALTER‐MAX‐VP2‐4‐3 or mixture with pALTER‐MAX‐ChIL‐6. The chickens at 4 weeks old were orally inoculated with vvIBDV SH95. The results showed that immunization with the mixture of pALTER‐MAX‐VP2‐4‐3 and pALTER‐MAX‐ChIL‐6 three times conferred protection for 90% of chickens. Enzyme‐linked immunosorbent assay (ELISA) antibody titres in chickens immunized together with pALTER‐MAX‐ChIL‐6 were higher than those immunized simply with plasmid pcDNA3‐VP2 or pALTER‐MAX‐VP2‐4‐3. IBDV was not detected in the bursa of the protected chickens at 8 days after challenge by RT‐PCR. The results indicate that protection against vvIBDV can be achieved by using the VP2‐4‐3 gene of vvIBDV as a DNA vaccine. Furthermore, the simultaneous injection of ChIL‐6 plasmid significantly increased the protection after challenge with the very virulent strain.  相似文献   

Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus          下载免费PDF全文

Brenda Y. Reeks  Franklin R. Champlin  Daniel B. Paulsen  Daniel W. Scruggs    Mark L. Lawrence 《Canadian journal of veterinary research》2005,69(1):1-10

The objective of this study was to determine the effects of sub-minimum inhibitory concentrations (sub-MICs) of 2 veterinary antibiotic preparations, chlortetracycline (CTC) and chlortetracycline-sulfamethazine (CTC + SMZ), on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus at normal and febrile body temperatures. Sub-minimum inhibitory concentrations of both antibiotics reduced the growth rates of M. haemolytica and H. somnus. Growth of both species was not inhibited when grown at 41 degrees C compared to 37 degrees C. There was no detectable consistent effect of antibiotic or temperature on outer membrane protein expression for either species. Our study indicates that sub-MIC levels of CTC and CTC + SMZ markedly impair growth of clinical M. haemolytica and H. somnus isolates, potentially allowing more effective host clearance during infection.  相似文献   

韩国一次性收获的红椒品种—Saeng-ryeog216     

M.C.Cho  D.H.pae  Y.S.Cho  Y.Chae  W.M.Lee  D.H.Kim 《辣椒杂志》2003,(4)

根据栽培面积以及为农民提供的收入而言,红辣椒在韩国一直是仅次于水稻而居第二位的重要农作物。按人均计算,韩国人均年辣椒粉的消费量达2.5kg以上,可能是世界最高记录。辣椒在韩国主要用作泡菜(韩国称之为Kimchi)香辛料和菜肴中的  相似文献   

Colonization of winter oilseed rape tissues by A/Tox⁺ and B/Tox⁰Leptosphaeria maculans (phoma stem canker) in France and England     

J. S. West †  M.-H. Balesdent  T. Rouxel  J. P. Narcy  Y.-J. Huang  J. Roux  J. M. Steed  B. D. L. Fitt  J. Schmit 《Plant pathology》2002,51(3):311-321

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Variation in the response of melon genotypes to Fusarium oxysporum f.sp. melonis race 1 determined by inoculation tests and molecular markers     

Y. Burger  N. Katzir  G. Tzuri  V. Portnoy  U. Saar  S. Shriber  R. Perl-Treves  R. Cohen † 《Plant pathology》2003,52(2):204-211

Screening of genotypes of melon ( Cucumis melo ) for resistance to wilt caused by Fusarium oxysporum f.sp. melonis is often characterized by wide variability in their responses to inoculation, even under carefully controlled conditions. The variability at the seedling stage of 17 genotypes susceptible to race 1 was examined in growth-chamber experiments. Disease incidence varied from 0 to 100% in a genotype-dependent manner. Using four combinations of light (60 and 90  µ E m⁻² s⁻¹) and temperatures of (27 and 31°C), only light intensity showed a statistically significant effect. Marker-assisted selection for fusarium resistance breeding using cleaved amplified polymorphic sequence (CAPS) and sequence-characterized amplified region (SCAR) markers were compared using a single set of genotypes that included 24 melon accessions and breeding lines whose genotype regarding the Fom-2 gene was well characterized. The practical value of the markers for discriminating a range of genotypes and clarifying the scoring of phenotypes was also tested using a segregating breeding population which showed codominant SCAR markers to be useful in marker-assisted selection.  相似文献