Biochemical affinities between Cucumis hystrix Chakr. and two cultivated Cucumis species (C. sativus L. and C. melo L.) based on isozyme analysis     

Jinfeng Chen  Shiro Isshiki  Yosuke Tashiro  Sadami Miyazaki 《Euphytica》1997,97(2):139-141

Isozymes of eight enzymes in Cucumis hystrix Chakr. and two cultivated Cucumis species (C. sativus L. and C. melo L.) were analysed electrophoretically to investigate the biosystematics of these three species. Cluster analysis using data from six enzymes indicated that considerable genetic distance existed between both C. hystrix and melon and between C. hystrix and cucumber. C. hystrix might be a key species for studying the evolution and taxonomy of genus Cucumis. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Development of a multiplex allele‐specific primer PCR assay for simultaneous detection of QoI and CAA fungicide resistance alleles in Plasmopara viticola populations     

Yoshinao Aoki  Yosuke Hada  Shunji Suzuki 《Pest management science》2013,69(2):268-273

BACKGROUND: DNA‐based diagnosis has become a common tool for the evaluation of fungicide resistance in obligate phytopathogenic fungus Plasmopara viticola. RESULTS: A multiplex allele‐specific primer PCR assay has been developed for the rapid detection of fungicide resistance in P. viticola populations. With this assay, a glycine‐to‐alanine substitution at codon 143 of the P. viticola cytochrome b gene, which conferred QoI fungicide resistance, and a glycine‐to‐serine substitution at codon 1105 of the P. viticola cellulose synthase gene PvCesA3, which conferred CAA fungicide resistance, were detected simultaneously. CONCLUSION: It is suggested that the present assay is a reliable tool for the rapid and simultaneous detection of QoI and CAA fungicide resistance alleles in P. viticola populations. The assay required only 2 h from the sampling of symptoms to the detection of resistance alleles to both fungicides. Copyright © 2012 Society of Chemical Industry  相似文献   

Valproic Acid Treatment from the 4-cell Stage Improves Oct4 Expression and Nuclear Distribution of Histone H3K27me3 in Mouse Cloned Blastocysts     

Yuuki ISAJI  Moeko MURATA  Naoya TAKAGUCHI  Toshita MUKAI  Yosuke TAJIMA  Hiroshi IMAI  Masayasu YAMADA 《The Journal of reproduction and development》2013,59(2):196-204

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Factors Influencing Early Survival and Growth of Laboratory‐reared Pacific Bluefin Tuna,Thunnus orientalis,Larvae          下载免费PDF全文

Yosuke Tanaka  Kazunori Kumon  Kentaro Higuchi  Takeshi Eba  Akefumi Nishi  Hideki Nikaido  Satoshi Shiozawa 《Journal of the World Aquaculture Society》2018,49(3):484-492

The collection of fertilized eggs for mass culture of Pacific bluefin tuna (PBT) relies on the spontaneous spawning of broodstock in captivity, and the spawning season of the broodstock is generally from mid‐May to September. The diameter of fertilized eggs from the broodstock decreases during the spawning season. To investigate the influence of three potential factors, the egg diameter (larger or smaller), the rearing water temperature (25 or 28 C), and the aeration rate during the night (strong or weak), on early survival and on growth of PBT larvae, replicate trials were conducted to assess these three factors until 7 d after hatching (d.a.h.). At 7 d.a.h., survival rates of larvae reared with strong nighttime aeration were found by a three‐way ANOVA to be significantly higher than for larvae reared with weak aeration. Furthermore, growth rates of PBT larvae hatched from larger eggs were significantly faster than those from smaller eggs. However, a significant difference in the survival rate was not detected for the factors, egg diameter and rearing water temperature. The results indicate that rearing with strong nighttime aeration significantly improved the early survival of PBT larvae and the egg diameter (a proxy for egg quality) and influenced growth.  相似文献   

Genotyping‐by‐sequencing for construction of a new genetic linkage map and QTL analysis of growth‐related traits in Pacific bluefin tuna          下载免费PDF全文

Tsubasa Uchino  Erina Hosoda  Yoji Nakamura  Motoshige Yasuike  Miyuki Mekuchi  Masashi Sekino  Atushi Fujiwara  Takuma Sugaya  Yosuke Tanaka  Kazunori Kumon  Yasuo Agawa  Yoshifumi Sawada  Motohiko Sano  Takashi Sakamoto 《Aquaculture Research》2018,49(3):1293-1301

Pacific bluefin tuna (Thunnus orientalis) has high market value, but its wild populations have decreased in recent years. The broodstock of Pacific bluefin tuna that were hatched artificially and reared under aquaculture conditions is beginning to be used for production. The creation of broodstock with commercially valuable traits, such as rapid growth, is therefore of great interest. Genetic linkage map‐based identification of markers associated with quantitative trait loci (QTLs) facilitates marker‐assisted selection (MAS) breeding and allows efficient genetic improvement of broodstock. Single nucleotide polymorphism (SNP)‐based genetic linkage map construction using the genotyping‐by‐sequencing method can expand the number of mapped markers and help identify growth‐related QTLs. In this study, we constructed sex‐specific maps for 24 linkage groups consisting of 677 SNP and 651 microsatellite markers. The total lengths of 93 progenies in the mapping population followed normal distribution, with an average length of 9.4 mm. We performed composite interval mapping in the mapping population. QTL analysis revealed one significant QTL in LG10 on the female linkage map. The genetic linkage map—the second such map generated for Pacific bluefin tuna—and the growth‐related QTLs detected in this study will be useful for tuna aquaculture MAS programs.  相似文献   

Onset of individual growth difference in larviculture of Pacific bluefin tuna <Emphasis Type="Italic">Thunnus orientalis</Emphasis> using fertilized eggs obtained from one female     

Takayuki Takebe  Takeo Kurihara  Nobuaki Suzuki  Kentaro Ide  Hideki Nikaido  Yosuke Tanaka  Satoshi Shiozawa  Hitoshi Imaizumi  Shukei Masuma  Yoshitaka Sakakura 《Fisheries Science》2012,78(2):343-350

We conducted a rearing experiment on Pacific bluefin tuna (PBT) larvae, which originated from one female broodstock, and determined the growth history of the larvae to clarify when the growth difference occurs. We investigated the otolith microstructure of the PBT larvae to examine the individual growth history and to predict the age and body size at the onset of individual growth difference. Since total length (TL) of fish can be back-calculated from otolith radius, we back-calculated TLs of 100 fish of 19 days post hatch (dph) divided into three size groups (small, intermediate, large). Growth difference was recognized from 3 dph (mouth opening), and the difference became larger thereafter. Growth of large-size fish was assumed to be promoted by the feeding conditions of PBT larvae.  相似文献   

Migration and spawning behavior of the greater amberjack Seriola dumerili in eastern Taiwan     

Kazuki Tone  Yosuke Nakamura  Wei-Chuan Chiang  Hsin-Ming Yeh  Sheng-Tai Hsiao  Chun-Huei Li  Kazuyoshi Komeyama  Masanori Tomisaki  Takamasa Hasegawa  Takashi Sakamoto  Itsumi Nakamura  Yoshitaka Sakakura  Ryo Kawabe 《Fisheries Oceanography》2022,31(1):1-18

Greater amberjack (Seriola dumerili) is an important fishery resource with a circumglobal distribution from tropical to temperate waters. Here, we investigated the spawning migration and habitat utilization of S. dumerili in the East China Sea (ECS). Archival tags were attached to 22 adult fish to examine their horizontal and vertical movements and estimate the spawning ground. S. dumerili were captured and released in the coastal waters of eastern Taiwan on November of 2016 and 2017. Information from seven pop-up satellite archival tags and seven depth–temperature recorders was recovered. Almost all of the fish stayed in the Taiwanese exclusive economic zone. Most individuals moved from released site to the southern edge of the ECS and showed behavior associated with the topographic features in winter (November to December). These phenomena may be related to foraging and be driven by oceanographic features such as the seasonal monsoon and the Kuroshio. The fish then migrated to the south offshore area of Taiwan in January and February. During their southward migration, the fish experienced a slowly elevated water temperature regime (SETR), which is one of the environmental factors that induce final oocyte maturation. In the spawning season (February to April), tagged females exhibited continuous diel vertical movements (DVMs) after experiencing the SETR. These continuous DVMs were observed over a wide geographic range from north to south in the Kuroshio off eastern Taiwan. Our study demonstrated that the putative spawning ground of S. dumerili must extend further in a north–south direction than predicted in a previous study.  相似文献   

Paragonimus ohirai metacercariae in crabs collected along the Arakawa River in Tokyo, Japan     

Sugiyama H  Morishima Y  Kameoka Y  Arakawa K  Kawanaka M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):927-931

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