Artificial insemination outcomes in beef females using bovine sperm with a detectable fertility-associated antigen     

Sprott LR  Harris MD  Forrest DW  Young J  Zhang HM  Oyarzo JN  Bellin ME  Ax RL 《Journal of animal science》2000,78(4):795-798

In this study, semen samples from 25 bulls that had passed a breeding soundness evaluation were analyzed for the presence or absence of a 31-kDa protein, known as fertility-associated antigen (FAA), on spermatozoal membranes. Eighteen bulls had FAA on sperm (FAA-positive) and seven were devoid of FAA on sperm (FAA-negative). A single ejaculate from each bull was extended and frozen with 25 to 30 x 10(6) sperm in .5-mL straws. Crossbred replacement heifers (n = 865) were estrus-synchronized and artificially inseminated either at timed AI or 12 h after they were detected in estrus. Mature cows (n = 285) were inseminated 12 h after they were detected in estrus during a 45-d AI period. Pregnancy rates (pooled) to first AI service for females (n = 764) inseminated with FAA-positive sperm were 65.6% and were 49.7% for females (n = 386) inseminated with FAA-negative sperm (P < .005). Among the estrus-synchronized replacement heifers, pregnancy rates to synchronized AI service for heifers (n = 550) inseminated with FAA-positive sperm were 62% and were 45.7% for heifers (n = 315) inseminated with FAA-negative sperm (P < .005). These data indicate that pregnancy rates to first AI service at spontaneous and synchronized estrus are higher when using semen from bulls with detectable FAA on spermatozoal membranes compared to semen from bulls devoid of FAA on membranes. Fertility-associated antigen is an important determinant for fertility potential of sperm from bulls to be used in AI breeding programs.  相似文献   

Regional brain monoamine concentrations and their alterations in bovine hypomagnesaemic tetany experimentally induced by a magnesium-deficient diet     

McCoy MA  Young PB  Hudson AJ  Davison G  Kennedy DG 《Research in veterinary science》2000,69(3):301-307

Monoamines are important brain neurotransmitters. An investigation was carried out to determine if hypomagnesaemic tetany was associated with alterations in regional brain monoamine concentrations in bovines. The results, established in cows with normal magnesium status, demonstrated that regional differences existed in the distribution and concentration of brain monoamines in the adult bovine, which were similar to those in other species. In magnesium-deficient cows, severe hypomagnesaemia and lowered cerebrospinal fluid (CSF) magnesium concentrations were associated with significant alterations in monoamine concentrations in some brain regions. Alterations in 3,4-dihydroxyphenylalanine (DOPA) and dihydroxyphenylacetic acid (DOPAC) concentrations in the corpus striatum, and dopamine (DA) in the cerebral cortex and cerebellum were recorded. These regions play an important role in both voluntary and involuntary motor function, and therefore these alterations may play a role in the aetiology of hypomagnesaemic tetany. However, there was no significant change in DA concentrations in the corpus striatum (the main dopaminergic region in the brain) associated with hypomagnesaemia. In addition, a significantly lower norepinephrine (NE) concentration in the corpus striatum of hypomagnesaemic animals was also recorded. Norephinephrine is generally excitatory and therefore lowered NE concentrations would be expected to result in depression rather than stimulation of motor function.  相似文献   

Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.   总被引：3，自引：0，他引：3  

Bo Kyu Kang  Dae Sub Song  Kwon Il Jung  Chul Seung Lee  Sung Jun Park  Jin Sik Oh  Dong Jun An  Jeong Sun Yang  Hyoung Joon Moon  Sang Sun Lee  Young Dhuk Yoon  Bong Kyun Park 《Journal of veterinary diagnostic investigation》2007,19(1):78-83

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Serologic and genetic characterization of North American H3N2 swine influenza A viruses          下载免费PDF全文

Marie Ren Gramer  Jee Hoon Lee  Young Ki Choi  Sagar M. Goyal    Han Soo Joo 《Canadian journal of veterinary research》2007,71(3):201-206

The H3N2 subtype of influenza A viruses isolated from pigs in the United States and Canada has shown both genetic and antigenic diversity. The objective of this study was to determine the serologic and genetic characteristics of contemporary strains of these viruses. Genetic analysis of 18 reference strains and 8 selected strains demonstrated differences in 1% to 9% of the nucleotides of the hemagglutinin (HA) gene. Phylogenetic analysis of the HA gene revealed 3 genetic clusters, as well as divergence of cluster III viruses from a cluster III prototype virus (A/Swine/Illinois/21587/99). By means of 1-way cross-hemagglutination inhibition with antiserum against 5 field isolates and 3 vaccine viruses, most of 97 isolates tested could be placed in 1 of 3 serogroups. The several isolates that did not react with any antiserum were in genetic cluster III, which suggests that continuous antigenic drift in cluster III may have resulted in virus variants. The efficacy of commercial vaccines against these virus variants should be evaluated with vaccination and challenge studies.  相似文献   

The expression and cellular localization of phospholipase D isozymes in the developing mouse testis     

Seungjoon Kim  Heechul Kim  Yongduk Lee  Jin Won Hyun  Young Ho Lee  Min Kyoung Shin  Do Sik Min  Taekyun Shin 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):209-212

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Effectiveness of 99mTc-tetrofosmin for assessment of heart functions in micropigs     

Min Young Lee  Sang Hun Lee  Jae Hong Park  Jung Sun Heo  Yu Jin Lee  Han Na Suh  Jung Jun Min  Young Soon Seo  Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):223-227

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Physiological Responses of Rainbow Trout Oncorhynchus mykiss to Crowding and Anesthesia with AQUI-S™     

Glen W.  Davidson Peter S.  Davie Graham  Young Reece T.  Fowler 《Journal of the World Aquaculture Society》2000,31(1):105-114

Abstract.— Following exposure to the anesthetic AQUI-S™, plasma cortisol concentration in immature rainbow trout was measured as (mean) 293 ± 48 ng/ mL, which was significantly ( P > 0.05) higher than the mean concentration in resting fish. Cortisol concentrations remained significantly ( P > 0.05) elevated for at least 24 h after treatment. This was accompanied by a significant increase and decrease in hematocrit and plasma potassium, respectively. These perturbations continued for at least 48 h following recovery from anesthesia. Plasma concentrations of total protein and sodium remained unchanged following anesthesia with AQUI-S™. Crowding stress is commonly encountered by fish during manipulation in aquaculture situations. Anesthetising fish prior to, and during, manipulation may reduce the associated stress. Changes in cortisol values resulting from crowding (30 min; 0.1 kg/L) during anesthesia with AQUI-S™ were not appreciably different from those in fish crowded without anesthesia. Thus, anesthesia with AQUI-S™ at the recommended dose of 17 mg/L did not appear to be effective for alleviating the stress of crowding under the conditions of our experiments.  相似文献   

The pathogenesis of velogenic Newcastle disease virus infection of chickens of different ages and different levels of immunity   总被引：2，自引：0，他引：2  

L Parede  P L Young 《Avian diseases》1990,34(4):803-808

Chickens of 7 weeks or 20 weeks of age were divided into three groups according to their antibody status (high, low, absent) and were infected with a velogenic viscerotropic Newcastle disease virus. To follow patterns of viral replication, birds were necropsied at regular intervals up to 22 days and organs were sampled from each bird. In non-immune birds, virus could be isolated from all organs examined. In birds with antibody, virus was most frequently isolated from the proventriculus, cecal tonsil, bursa, and brain. However, because no one organ could be recommended for all situations, all four should be sampled for field diagnosis. In immune birds, although clinical signs were either mild or absent, widespread virus replication occurred up to 19 days post-challenge.  相似文献   

Effect of ACTH on plasma corticosterone and cortisol in eagles and condors   总被引：1，自引：0，他引：1  

R D Zenoble  R J Kemppainen  D W Young  J W Carpenter 《Journal of the American Veterinary Medical Association》1985,187(11):1119-1120

The effect of ACTH on plasma corticosterone and cortisol was determined in 12 eagles (Haliaeetus leucocephalus) and in 6 Andean condors (Vultur gryphus). In all raptors, the concentration of plasma corticosterone was substantially greater than that of cortisol. After ACTH administration, the eagles had a marked increase (P less than 0.001) in plasma corticosterone concentrations, but not in plasma cortisol. Administration of saline solution did not induce increased plasma corticosterone concentrations in the eagles. The condors had a smaller increase (P less than 0.002) in plasma corticosterone concentrations after ACTH administration, as compared with that of the eagles. However, administration of saline solution in 2 condors resulted in an increase in corticosterone similar to the increase after ACTH administration. In the condor, a stress-related release of endogenous ACTH may have an effect similar to that induced by exogenously administered ACTH. Plasma cortisol concentrations did not increase significantly after administration of ACTH or saline solution in either raptor species.  相似文献   

Cardiorespiratory responses to electrical stimulation of the buccal mucosa in ponies   总被引：1，自引：0，他引：1  

S S Young 《Research in veterinary science》1990,49(3):268-274

The cardiovascular and respiratory responses to electrical stimulation of the buccal mucosa under general anaesthesia in ponies were measured in order to provide some insight into the cardiorespiratory effects of anaesthesia in equidae. This knowledge may be useful for reducing morbidity during clinical anaesthesia in horses. Anaesthesia was induced with intravenous thiopentone and maintained with 1.3 per cent inspired halothane in oxygen. Arterial blood pressure, heart rate, minute volume, tidal volume, respiratory rate, arterial blood gas tensions and clinical signs of anaesthetic depth were recorded while the buccal mucous membrane was stimulated electrically. A rise in arterial blood pressure was the most consistent response detected along with clinical signs. The response of individual animals varied considerably. Alterations of respiratory pattern sometimes occurred during stimulation but there was no consistent pattern of change in any animal.  相似文献