Ubiquitous increase in taurine transporter mRNA in tissues of tilapia (Oreochromis mossambicus) during high-salinity adaptation     

K. Takeuchi  H. Toyohara  M. Kinoshita  M. Sakaguchi 《Fish physiology and biochemistry》2000,23(2):173-182

We have isolated a cDNA encoding the taurine transporter from a tilapia (Oreochromis mossambicus) gill cDNA library. Transient expression of the cDNA in COS-7 cell indicates that the clone encodes a Na⁺- and Cl^–-dependent and -amino acid-specific taurine transporter. By the transfer of tilapia cultured in freshwater to 70% artificial seawater, plasma osmolality increased by up to 100–135 mOsm/kgH₂O along with the marked increase in the taurine transporter mRNA level in all the tissues examined i.e., kidney, stomach, intestine, gill, eye, liver, fin, muscle and brain. In most tissues, time-dependent change in the taurine transporter mRNA level corresponds to that in plasma osmolality. However, fin showed an acute and muscle showed a delayed increase in taurine transporter mRNA compared to changes in plasma osmolality. The taurine transporter mRNA level in tilapia embryos also increased after transfer from freshwater to 100% artificial seawater. Increase in taurine transporter expression leads to the activation of cellular uptake of taurine from plasma and the accumulation of taurine in the cell. Thus the results in the present study suggest that taurine plays an important role as an osmolyte in the ubiquitous tissues of tilapia during high-salinity adaptation.  相似文献   

Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution     

Yasuyuki Onodera  Takumi Arakawa  Rika Yui-Kurino  Masayuki P. Yamamoto  Kazuyoshi Kitazaki  Shigehiko Ebe  Muneyuki Matsunaga  Kazunori Taguchi  Yosuke Kuroda  Shiko Yamashita  Tomoyuki Sakai  Toshiro Kinoshita  Tetsuo Mikami  Tomohiko Kubo 《Euphytica》2015,206(2):365-379

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A network of superconducting gravimeters detects submicrogal coseismic gravity changes   总被引：8，自引：0，他引：8  

Imanishi Y  Sato T  Higashi T  Sun W  Okubo S 《Science (New York, N.Y.)》2004,306(5695):476-478

With high-resolution continuous gravity recordings from a regional network of superconducting gravimeters, we have detected permanent changes in gravity acceleration associated with a recent large earthquake. Detected changes in gravity acceleration are smaller than 10(-8) meters seconds(-2) (1 micro-Galileo, about 10(-9) times the surface gravity acceleration) and agree with theoretical values calculated from a dislocation model. Superconducting gravimetry can contribute to the studies of secular gravity changes associated with tectonic processes.  相似文献   

Magnetic properties of the lunar crystalline rock and fines     

Nagata T  Ishikawa Y  Kinoshita H  Kono M  Syono Y  Fisher RM 《Science (New York, N.Y.)》1970,167(3918):703-704

Magnetic measurements have shown that nondiamagnetic minerals in a lunar crystalline rock of type B are (free Fe(2)+ in paramagnetic pyroxenes) : (antiferromagnetic FeSiO(3)) : (antiferromagnetic FeTiO(3)) : (ferromagnetic iron) = 4.3 : 7 : 20 : 0.08 in weight percentage. The abundance of ferromagnetic Fe in the lunar fines is about 7.5 times its abundance in the crystalline rock. The natural remanent magnetization of the crystalline rock of 7.5 x 10(-6) emu/ g in intensity may not be attributable to its thermoremanent magnetization.  相似文献   

Subaru telescope observations of Deep Impact     

Sugita S  Ootsubo T  Kadono T  Honda M  Sako S  Miyata T  Sakon I  Yamashita T  Kawakita H  Fujiwara H  Fujiyoshi T  Takato N  Fuse T  Watanabe J  Furusho R  Hasegawa S  Kasuga T  Sekiguchi T  Kinoshita D  Meech KJ  Wooden DH  Ip WH  A'Hearn MF 《Science (New York, N.Y.)》2005,310(5746):274-278

The impact cratering process on a comet is controversial but holds the key for interpreting observations of the Deep Impact collision with comet 9P/Tempel 1. Mid-infrared data from the Cooled Mid-Infrared Camera and Spectrometer (COMICS) of the Subaru Telescope indicate that the large-scale dust plume ejected by the impact contained a large mass (approximately 10(6) kilograms) of dust and formed two wings approximately +/-45 degrees from the symmetric center, both consistent with gravity as the primary control on the impact and its immediate aftermath. The dust distribution in the inner part of the plume, however, is inconsistent with a pure gravity control and implies that evaporation and expansion of volatiles accelerated dust.  相似文献   

One-way control of FWA imprinting in Arabidopsis endosperm by DNA methylation   总被引：1，自引：0，他引：1  

Kinoshita T  Miura A  Choi Y  Kinoshita Y  Cao X  Jacobsen SE  Fischer RL  Kakutani T 《Science (New York, N.Y.)》2004,303(5657):521-523

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Counting on science to compete     

Kinoshita J 《Science (New York, N.Y.)》1993,262(5132):348-350

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AID enzyme-induced hypermutation in an actively transcribed gene in fibroblasts     

Yoshikawa K  Okazaki IM  Eto T  Kinoshita K  Muramatsu M  Nagaoka H  Honjo T 《Science (New York, N.Y.)》2002,296(5575):2033-2036

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Molecular characterization of the major outer membrane protein of Haemophilus somnus     

Khan MS  Tanaka A  Ide H  Hoshinoo K  Hanafusa Y  Tagawa Y 《Veterinary microbiology》2005,107(3-4):179-192

The major outer membrane protein (MOMP) of Haemophilus somnus shows antigenic and molecular mass diversity that forms the basis of a preliminary grouping system for H. somnus strains. In this study, the gene encoding MOMP of H. somnus strain 8025 was cloned in three overlapping fragments by PCR techniques, and then sequenced. The gene consists of a 1164-bp open reading frame encoding a deduced 380-amino acid protein with a 19-amino acid signal sequence, giving a mature protein with a calculated molecular mass of 39,913 Da. Significant homology was found between MOMP and porin protein sequences of bacteria in Pasteurellaceae species. When expressed in Escherichia coli, the protein from the MOMP gene directed by the T7 promoter was identical in size (approximately 40 kDa) to native MOMP and reacted with MOMP-specific antibodies. Comparisons of the MOMP gene sequences from six unrelated strains of H. somnus to that of strain 8025 revealed that the genes of three MOMP type 1 strains were highly conserved with that of strain 8025 in length and sequence. However, two MOMP type 3c strains and one MOMP type 3a strain differed markedly from the MOMP of strain 8025 in their 3'-terminal halves. Their deduced MOMP amino acid sequences differed in sequence (3c, 80.5 and 82.7% identity; 3a, 62.4% identity) and in length (3c, 384 and 376; 3a, 316), indicating that the molecular differences are the basis of antigenicity and molecular mass differences of H. somnus MOMP. In the predicted MOMP secondary structure, the variable sequences primarily mapped to putative surface-exposed loops, and a variable and surface-exposed epitope of MOMP-specific antibody was identified in the seventh-largest loop. These findings are useful for understanding the structural and immunological characteristics of H. somnus.  相似文献   

Prediction from ultrasonographic measurements of the expected delivery date in two species of bottlenosed dolphin (Tursiops truncatus and Tursiops aduncus)     

Lacave G  Eggermont M  Verslycke T  Brook F  Salbany A  Roque L  Kinoshita R 《The Veterinary record》2004,154(8):228-233

Ultrasonographic measurements were made at least once a month during 14 gestations in seven Tursiops truncatus and 12 gestations in five Tursiops aduncus (bottlenosed dolphins). The 121 measurements of the fetal biparietal diameter and 139 measurements of the fetal thoracic diameter in T truncatus and the 97 measurements of the biparietal diameter and 97 measurements of the thoracic diameter in Taduncus were used to establish regression lines for the increases in the diameter of the head and thorax of the fetus with time. From these relationships an easy-to-use computer program was developed to predict the date of birth of the two species of bottlenosed dolphin, and its predictions were compared with the actual dates of birth of other calves of both species. The births occurred within the range of predicted dates, and even when only a few measurements were available, the program provided accurate predictions.  相似文献