西藏牦牛和黑白花奶牛生长激素基因AluⅠ多态性的比较研究   总被引：10，自引：0，他引：10  

陈桂芳  李齐发  强巴央宗  土登  谢庄 《畜牧与兽医》2002,34(11):15-16

利用聚合酶链式反应 (PCR)技术 ,测定了西藏牦牛和黑白花奶牛生长激素基因的AluⅠ RFLP (限制性内切酶片段长度多态性 ) ,并比较了2牛群的基因频率。在西藏牦牛和黑白花奶牛中 ,等位基因A的频率分别为 0 935和 0 90 5 ,等位基因B的频率分别为 0 0 65和 0 0 65 ,卡方检验差异不显著 (P >0 0 5)。  相似文献   

High-Frequency Jet Ventilation in Anesthetized, Paralyzed Dogs and Cats Via Transtracheal and and Endotracheal Tube Routes     

Steve C. Haskins DVM  MS  H. Orima  Y. Yamamoto  John D. Patz BA 《Journal of Veterinary Emergency and Critical Care》1991,1(2):55-60

The ability of the SAV 6 high-frequency jet ventilator to effectively ventilate three anesthetized, paralyzed cats (3.2–4.2 kg), two small dogs (7.2 and 10.0 kg), six medium-sized dogs (20.5–25.0 kg), and three large dogs (36.0–43.0 kg) via a 14-gauge (dogs) or a 16-gauge (cats) catheter placed percutaneously into the trachea via the cricothyroid membrane or into a preplaced endotracheal tube was evaluated. The lowest driving pressure within the range of 0.25 to 2.0 kg/cm² (1 kg/cm²= 14.2 psi) and the highest cycle rate within the range of 60 to 240 per minute that would generate a PaCO₂ of 30 ± 3 mm Hg were determined.
All animals could be ventilated to a PaC0₂ of 30 ± 3 mm Hg by the endotracheal tube and transtracheal route, except the largest dogs, which couid be ventilated to an average PaC0₂ of 36 mm Hg by the transtracheal route. The transtracheal route consistently required higher driving pressures and lower cycle rates than did the endotracheal tube route. Cats could be ventilated with a driving pressure of 0.25 kg/cm²; small dogs could be ventilated with 0.5 to 1.0 kg/cm₂; medium-sized dogs with 1.0 to 1.5 kg/cm²; and large dogs with 1.5 to 2.0 kg/cm².
The SAV 6 high-frequency jet ventilator can effectively ventilate cats and dogs (7.2–43.0 kg) via a transtracheal catheter and an endotracheal tube.  相似文献   

X-Chromosomal Monosomy (77, XO) in a Doberman Pinscher With Gonadal Dysgenesis     

F. W. K. Smith Jr  DVM  L. C. Buoen BA    A. F. Weber DVM  PhD    S. D. Johnston DVM  PhD    J. F. Randolph DVM  D. J. Waters DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1989,3(2):90-95

A 6-month-old stunted female Doberman Pinscher was found to have a 77,X0 chromosomal complement. The ovaries were small, consisting primarily of interstitial-type cells and solid epithelial cords. The dam, sire, and a male littermate had normal karyotypes.  相似文献   

Comparison of the properties of hyaluronate products used in treatment of joint dysfunction in horses     

Sandra A. Loftus BA  Jill M. Montplaisir BS  Jing-wen Kuo MS  Dale P. DeVore PhD   《Journal of Equine Veterinary Science》1988,8(2)

A standardized investigation was conducted to compare the biophysical properties of hyaluronate products used in the treatment of joint dysfunction in horses. Results showed that two of the five products, Hyvisc® and Hylartin®V, contained higher-weight molecular hyaluronate (greater than 1.5×10⁶). Other investigations have shown that higher molecular weight hyaluronate is effective in providing physical resistance to shear, in stimulating endogenous hyaluronate synthesis, and in inhibiting excess cell proliferation which may be associated with synovial pannus formation. It is suggested that the therapeutic benefits of hyaluronate products may be dependent on the molecular size of the hyaluronate molecules. Higher molecular weight hyaluronate products would be expected to be more effective than lower molecular hyaluronate products in providing therapeutic benefits.  相似文献   

Observations and immunohistochemical detection of Coronavirus, Cryptosporidium parvum and Giardia intestinalis in neonatal diarrhoea in lambs and kids     

Ozmen O  Yukari BA  Haligur M  Sahinduran S 《Schweizer Archiv für Tierheilkunde》2006,148(7):357-364

In this study, clinical, parasitological, macroscopical, histopathological and immunohistochemical examinations were performed on 19 kids and 11 lambs (30 animals) with neonatal diarrhoea to detect the presence of Coronavirus, Cryptosporidium parvum and Giardia intestinalis. Clinically, severe dehydration, yellowish-green to brown coloured diarrhoea and death were observed. Mortality rates were 10-30% in the examined flocks. The most common agent was C. parvum diagnosed in 20 animals as a single causative agent, whereas G. intestinalis was found in 5 of 30 animals. These two protozoa were detected together in 4 animals upon faeces examination. Fifteen of 24 cases of C. parvum and 3 of 11 cases of G. intestinalis were also confirmed histopathologically. Following immunohistochemical examination, all cryptosporidiosis cases were confirmed by positive immunostaining of intestinal sections. Two additional Giardiosis cases with negative results upon parasitological and histopathological examinations were diagnosed by means of immunohistochemical examination. Coronavirus was detected immunohistochemically in one kid with neonatal enteritis. Following diagnosis, herds were treated with Trimethoprim + Sulfodoxine and multivitamin complexes. Intravenous and intramuscular administrations of these drugs were effective for both treatment and prevention of neonatal diarrhoea in lambs and kids.  相似文献   

Polymorphisms in the canine glutamate transporter-1 gene: identification and variation among five dog breeds     

Ogata N  Hashizume C  Momozawa Y  Masuda K  Kikusui T  Takeuchi Y  Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):157-159

Excitatory amino acid transporters (EAATs) are important for terminating glutamatergic neurotransmission and protect central nervous system (CNS) neurons from glutamatergic excitotoxicity. We selected these genes as targets that may relate to canine behavioral traits. After screening four EAAT genes (glutamate transporter-1; GLT-1, excitatory amino acid transporter 4; EAAT4, excitatory amino acid carrier; EAAC1, glutamate/aspartate transporter; GLAST) for single nucleotide polymorphisms (SNPs), we identified two silent SNPs (C129T and T471C) in the GLT-1 gene. We genotyped 193 dogs of 5 breeds and found significant variation among breeds in these two SNPs in GLT-1. The C129T polymorphism was not observed in Malteses and Miniature Schnauzers. These results suggest that polymorphisms in the GLT-1 gene may be useful markers for examining how the genetic background relates to the behavioral traits of dogs.  相似文献   

A comparison of the behavioral profiles of purebred dogs in Japan to profiles of those in the United States and the United Kingdom     

Takeuchi Y  Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):789-796

To clarify the behavioral profiles of 56 pure breeds of dogs in Japan, 96 small-animal veterinarians participated in a questionnaire survey using the same criteria as in preceding studies conducted in the United States and the United Kingdom. We found significant differences among breeds in all behavioral traits examined. In addition, gender differences were revealed in terms of aggression to dogs, territorial defense, excitability, general activity, dominance over owner, destructiveness, watchdog barking, and snapping at children, which were all rated higher in males than females, whereas obedience training and housebreaking ease were rated higher in females. No gender differences were evident in playfulness, excessive barking, or affection demand. Using factor analyses, "aggressiveness", "reactivity", and "trainability" were determined to be consistent with results found in the US and UK surveys. On the basis of these factor scores, seven groups of breeds were determined by cluster analysis to compare to the US survey; 22 of the 38 breeds common with the US survey were categorized into the same groups as those in that survey. The results demonstrated differences in canine behavioral predisposition among breeds and between genders. The similarity in the results between our study and previous surveys, which involved distinct geographical locales, suggests that the genetic basis of breed-specific temperamental traits is manifested irrespective of the cultural or regional identities of the owners.  相似文献   

羊踯躅嫩叶离体培养和植株高效再生技术研究     

顾地周  陆爽  巴春影 《西北农林科技大学学报(自然科学版)》2012,40(6):189-194

【目的】建立羊踯躅嫩叶离体培养及植株高效再生体系。【方法】以羊踯躅新生嫩叶为外植体,筛选嫩叶愈伤组织诱导、再分化及生根培养基,并以再生植株茎节为试材,建立高效植株再生体系。【结果】最适合羊踯躅嫩叶愈伤组织诱导的培养基为1/2 DR+2.40 mg/L 2ip+0.80 mg/L NAA,诱导率为99.5%;愈伤组织再分化的最佳培养基为1/2 DR+2.90 mg/L 2ip+0.01 mg/L NAA+1.75 mg/L KT,分化率为99.7%;适宜生根的培养基为1/4 DR+0.06 mg/L ZT+0.02 mg/L NAA,生根率达99.0%。利用再生植株茎节的快繁结果表明,在28 d的培养周期内,每节段平均增殖达5倍以上。【结论】成功建立了羊踯躅嫩叶愈伤组织诱导、再分化芽苗和植株高效再生体系,可以满足羊踯躅工厂化育苗的需要。  相似文献   

外源物质对辣椒种子发芽的影响研究     

杨茹薇  巴合旦  秦勇  申耀荣 《种子》2012,31(6):100-102

用一定浓度的赤霉素(GA3)、生长素(IAA)、过氧化氢(H2O2)、硝酸钾(KNO3)、萘乙酸(NAA)溶液处理可显著提高辣椒砧木品种肩车种子的发芽率,并对其发芽势、发芽指数有一定的促进作用。比较几种药剂对辣椒种子发芽的影响发现,GA3和IAA对辣椒种子的发芽影响效果最佳,最适宜浓度分别为200 mg/L和1.5 mg/L。  相似文献   

铜、镉污染对小麦种子萌发及幼苗生长的影响   总被引：1，自引：0，他引：1  

吕笃康  巴音山  赵玉 《种子》2012,31(9):108-111

以小麦种子为试材,通过水培试验研究了铜、镉污染对小麦种子萌发及幼苗生长的影响。结果表明,在实验所选的胁迫浓度范围内,单一铜污染时,对小麦种子萌发初期有短暂的促进作用,小麦种子发芽率随着Cu2+浓度的升高,呈先升后降。在20 mg/L Cu2+处理时出现了毒性兴奋效应现象。铜、镉污染对小麦幼苗的苗长和根长的抑制作用较为显著。根据综合效应指标,铜、镉复合污染对小麦种子萌发的影响表现形式为铜、镉的协同作用。  相似文献