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Naomi NAKAGATA Toru TAKEO Kiyoko FUKUMOTO Yukie HARUGUCHI Tomoko KONDO Yumi TAKESHITA Yuko NAKAMUTA Tomoko UMENO Shuuji TSUCHIYAMA 《The Journal of reproduction and development》2014,60(2):168-171
Sperm cryopreservation has been widely adopted for maintenance of the genetically
engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes
worldwide. However, the recipients are not always able to obtain high fertilization rates
with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm
via various methods and performed in vitro
fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm
preincubation and reduced glutathione for insemination was used (the MBCD-GSH IVF). In
addition, frozen sperm sent from the Jackson Laboratory (USA) were thawed and used for IVF
in the same manner. The fertilization rates of both the sperm cryopreserved
via the methods applied in some countries and the cryopreserved GEM
sperm improved when used with the MBCD-GSH IVF method. Therefore, we strongly believe that
the MBCD-GSH IVF method brings about relatively high fertilization rates with any strain
of frozen mouse sperm. 相似文献
33.
Ito K Asano K Urano T Ogiwara N Seki M Kato Y Sasaki Y Teshima K Kutara K Edamura K Shibuya H Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):747-748
A 4-year-old female Miniature Dachshund was referred with a chief complaint of right periorbital swelling that had not responded to antibiotic therapy. Ultrasonography and fine-needle aspiration revealed that the periorbital lesion had a cystic structure without any inflammatory or neoplastic cells. Computed tomography (CT) showed that the cyst occupied a defect in the periorbital maxillary, lacrimal, and frontal bones and had invaded the nasal cavity. The lesion was histologically suspected by incisional biopsy as an epithelial cyst. 相似文献
34.
Sasaki K Oguma S Glass T Namiki Y Sugiyama H Ohmura N Blake DA 《Journal of agricultural and food chemistry》2008,56(17):7613-7616
In order to develop a rapid inexpensive test for cadmium in rice, we identified an antibody specific for cadmium-EDTA complexes; this antibody binds to cadmium-EDTA with a Kd of approximately 10(-8) M. Although the antibody's cross reactivity to magnesium was minimal (Kd approximately 10(-5) M), the high toxicity of cadmium coupled with the high natural occurrence of magnesium in rice resulted in a situation where magnesium interfered with cadmium determination and resulted in falsely elevated estimates of cadmium. Fortunately, the formation constant of EDTA for cadmium is approximately 5 x 10(7) times higher (at pH 7) than the formation constant of EDTA for magnesium, and we were able to eliminate the magnesium interference by judicious selection of the EDTA concentration used in the assay. The resulting equilibria are complex, but we show that a relatively simple two-step model in which cadmium and magnesium compete for EDTA followed by cadmium-EDTA and magnesium-EDTA competing for antibody provided a good fit to the measured data. These analyses enabled appropriate selection of the optimum EDTA concentration for an immunoassay with improved selectivity. 相似文献
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Yukie Tada Norio Yano Hiroshi Takahashi Katsuhiro Yuzawa Hiroshi Ando Yoshikazu Kubo Akemichi Nagasawa Akio Ogata Dai Nakae 《Journal of toxicologic pathology》2012,25(4):233-239
Iron nanomaterials are of considerable interest for application to
nanotechnology-related fields including environmental catalysis, biomedical imaging, drug
delivery and hyperthermia, because of their superparamagnetic characteristics and high
catalytic abilities. However, information about potential risks of iron nanomaterials is
limited. The present study assessed pulmonary responses to a single intratracheal spray
instillation of triiron tetraoxide nanoparticles (magnetite) in rats. Ten-week-old male
and female Fischer 344 rats (n=5/group) were exposed to a single intratracheal spray
instillation of 0 (vehicle), 5.0, 15.0 or 45.0 mg/kg body weight (BW) of magnetite. After
14 days, the rats were sacrificed, and biological consequences were investigated. The lung
weights of the 15.0 and 45.0 mg/kg BW male and female groups were significantly higher
than those of the control groups. The lungs of treated rats showed enlargement and black
patches originating from the color of magnetite. The typical histopathological changes in
the lungs of the treated rats included infiltration of macrophages phagocytosing
magnetite, inflammatory cell infiltration, granuloma formation and an increase of goblet
cells in the bronchial epithelium. The results clearly show that instilled magnetite
causes foreign body inflammatory and granulating lesions in the lung. These pulmonary
responses occur in a dose-dependent manner in association with the increase in lung
weight. 相似文献
36.
Tanaka T Abe Y Inoue N Kim WS Kumura H Nagasawa H Igarashi I Shimazaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):619-625
Trypanosoma brucei, the causative agent of sleeping sickness in humans, requires transferrin (TF) for growth. Therefore, T. brucei has a TF receptor that allows it to obtain iron from TF. Lactoferrin (LF), a member of the TF family protein, is an iron-binding protein that is found in most biological fluids of mammals. LF has been shown to interact with some bacteria species by specific receptor-ligand binding. We examined the ability of T. brucei to bind bovine LF (bLF) by using a fluorescence test, streptavidin-biotin (SAB) microplate analysis, and far Western blotting using a biotin-streptavidin system. We found that bLF bound to components of T. brucei, and that bLF hydrolysate disrupted the sites responsible for binding to parasite proteins. Furthermore, bLF, human LF, bovine TF, and ovotransferrin bound same proteins of T. brucei, which exhibited molecular masses of 40 and 43 kDa. The N-terminal amino acid sequence of the 40 kDa bLF binding protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). 相似文献
37.
Asano K Watari T Kuwabara M Sasaki Y Teshima K Kato Y Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1269-1272
A Miniature Dachshund, 3-month-old, 3.1 kg, was diagnosed as an intrahepatic portosystemic shunt (PSS) with the shunting vessel in 6-mm diameter. Percutaneous transvenous coil embolization (PTCE) was performed with a stainless steel coil in 8-mm diameter. Intraoperative portal pressure elevated about 2.5 times after one-stage coil occlusion. Two weeks after the PTCE, serum bile acid levels reduced within the normal range. The portogram showed complete occlusion of the shunting vessel 4 months after the PTCE. Approximately 3 years after the PTCE, the patient has shown no clinical signs. PTCE could be performed more easily and less invasively in a small-breed dog. It is therefore suggested that PTCE is a promising therapeutic technique in canine intrahepatic PSS. 相似文献
38.
Teshima K Asano K Sasaki Y Kato Y Kutara K Edamura K Hasegawa A Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(12):1207-1215
Pulsed tissue Doppler imaging (pulsed TDI) has been demonstrated to be useful for the estimation of left ventricular (LV) systolic and diastolic functions in various human cardiac diseases. The objectives of this study were to investigate the relationship between pulsed TDI and LV function by using cardiac catheterization in healthy dogs and to evaluate the clinical usefulness of pulsed TDI in dogs with spontaneous mitral regurgitation (MR). The peak early diastolic velocity (E'), peak atrial systolic velocity (A'), and peak systolic velocity (S') were detectable in the velocity profiles of the mitral annulus in all the dogs. In the healthy dogs, S' and E' were correlated with LV peak +dP/dt and -dP/dt, respectively. E' was lower in dogs with MR than in dogs without cardiac diseases. E/E' in the MR dogs with decompensated heart failure was significantly increased in comparison with those with compensated heart failure. The sensitivity and specificity of the E/E' cutoff value of 13.0 for identifying decompensated heart failure were 80% and 83%, respectively. In addition, E/E' was significantly correlated with the ratio of left atrial to aortic diameter. These findings suggest that canine pulsed TDI can be applied clinically for estimation of cardiac function and detection of cardiac decompensation and left atrial volume overload in dogs with MR. 相似文献
39.
A pilot study evaluating adaptive closed‐loop fluid resuscitation during states of absolute and relative hypovolemia in dogs 
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Yamashita K Ueyama Y Miyoshi K Igarashi R Kushiro T Umar MA Muir WW 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):43-47
Minimally invasive cardiac output was determined using transthoracic bioimpedance (BICO), partial carbon dioxide rebreathing (NICO) and transesophageal Doppler echocardiography (TEECO) and compared to thermodilution (TDCO) in 6 beagle dogs. The dogs were 2 years old, weigh between 9.1-13.0 kg and were anesthetized with nitrous oxide-oxygen-sevoflurane. All dogs were administered a neuromuscular blocking drug and artificially ventilated during anesthesia. Thirty paired measurements of TDCO and each non-invasive method were collected during low, intermediate, and high values of cardiac output achieved by varying the depth of anesthesia and the administration of dobutamine. Cardiac output values ranged from 1.10-2.50 L/min for BICO compared to 0.81-4.88 L/min for TDCO; 0.70-2.60 L/min for NICO compared to 0.89-4.45 L/min for TDCO; and 0.59-4.37 L/min for TEECO compared to 0.57-4.15 L/min for TDCO. The limits of agreement and percentage error were -0.58 +/- 1.56 L/min and +/- 75.4% for BICO, -1.04 +/- 1.08 L/min and +/- 56.0% for NICO, and 0.03 +/- 0.26 L/min and +/- 12.3% for TEECO compared to TDCO. In conclusion, TEECO provided the best agreement to TDCO in sevoflurane anesthetized beagle dogs. 相似文献