Properties of scallop mantle collagen: its content,tissue distribution and thermal behavior     

Shoshi Mizuta  Yuko Nakanishi  Masashi Shiraishi  Yoshihiro Yokoyama  Reiji Yoshinaka 《Fisheries Science》2007,73(6):1353-1361

ABSTRACT:   To obtain fundamental information for the effective use of scallop Patinopecten yessoensis mantle, which is one of the underutilized marine resources. Some properties of collagen contained in the mantle were examined by chemical and histochemical techniques. Collagen content in the mantle varied annually, ranging from 0.98 to 1.72% of wet tissue, 7.7 to 12.6% of dry tissue and 13.5 to 26.5% of total protein, being relatively in high level of collagen content of invertebrate muscles. Collagen fiber was densely distributed in the inner connective tissue matrix of the mantle pallial, in contrast to the inner fold part which was rich in muscle fibers. The collagen contained in the crude collagen fraction (residue after alkali extraction), prepared from the mantle, was revealed to have considerably low solubility on hot-water extraction, constantly less than 20% of the total collagen at the temperatures in the range of 20–90°C.  相似文献   

Phase I Dose-Escalation Study of Nimustine in Tumor-Bearing Dogs     

Masashi TAKAHASHI  Yuko GOTO-KOSHINO  Kenjiro FUKUSHIMA  Hideyuki KANEMOTO  Ko NAKASHIMA  Yasuhito FUJINO  Koichi OHNO  Yasuyuki ENDO  Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):895-899

  相似文献   

Epigenetic regulation of the ABCB1 gene in drug-sensitive and drug-resistant lymphoid tumour cell lines obtained from canine patients     

Hirotaka Tomiyasu  Yuko Goto-Koshino  Yasuhito Fujino  Koichi Ohno  Hajime Tsujimoto 《Veterinary journal (London, England : 1997)》2014,199(1):103-109

  相似文献   

Oral Administration of Fermented Probiotics Improves the Condition of Feces in Adult Horses     

Saori ISHIZAKA  Akira MATSUDA  Yosuke AMAGAI  Kumiko OIDA  Hyosun JANG  Yuko UEDA  Masaki TAKAI  Akane TANAKA  Hiroshi MATSUDA 《Journal of Equine Science》2014,25(4):65-72

  相似文献   

Characterization of cystathionine λ-lyase in the liver of rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>     

Takanobu?GotoEmail author  Yuko?Osada  Hiroyuki?Funatsu  Hiroki?Sugiyama  Shusaku?Takagi  Akihiko?Mochizuki 《Fisheries Science》2005,71(1):245-247

  相似文献   

Effect of taurine supplemented practical diet on growth performance and taurine contents in whole body and tissues of juvenile Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>     

Shin-Kwon?Kim  Toshio?TakeuchiEmail author  Atsushi?Akimoto  Hirofumi?Furuita  Takeshi?Yamamoto  Masahito?Yokoyama  Yuko?Murata 《Fisheries Science》2005,71(3):627-632

ABSTRACT:   The effect of dietary taurine on juvenile Japanese flounder was determined by feeding three taurine-supplemented experimental diets (TAU) and a commercial diet (CD) to evaluate a practical diet for juvenile Japanese flounder. Juvenile Japanese flounder were reared on the three experimental diets supplemented with taurine at 0, 0.5, 1.0% and CD. These diets were fed to juvenile Japanese flounder of an initial mean body weight of 0.2 g for 6 weeks at 20°C and the taurine contents of the whole body and tissues were analyzed. The final average body weight of juvenile Japanese flounder fed the 1.0% TAU was significantly higher than that of the other groups. Taurine contents in the whole body and tissues increased with the increase in dietary taurine level. These results indicate that juvenile Japanese flounder require at least 15 mg/g taurine in the diet, even though a combined mix of fish, krill and squid meal was the main protein source in the experimental diets.  相似文献   

Assessing the potential impacts of climate change and their conservation implications in Japan: A case study of conifers     

Yuko Ogawa-Onishi  Pam M. Berry 《Biological conservation》2010,143(7):1728-1736

The Japanese archipelago is a biodiversity hotspot with a unique regional climate influenced by the Asian monsoon circulation, surrounding seas, and complex topography. Japan has numerous mountains and islands, which are potentially vulnerable to climate change. This study evaluated the potential impact of climate change on species diversity in Japan, using 25 conifer tree species as a case study. We applied ensemble models based on generalised additive models, artificial neural networks, generalised boostedmodels, and random forests to species’ locality records at 1 km² resolution. The results indicated a substantial impact, such that 80% of the species may lose over half of their current climatically suitable areas by 2100. The lower altitudinal range limits were projected to shift upwards by 293 m on average, suggesting that alpine/sub-alpine and sub-boreal species may face widespread local extinctions. The impacts on sub-alpine species may be moderated by assisted migration to the northern island where they currently do not occur. However, climatically suitable areas for these species and sub-boreal species that occur on the island overlapped significantly, indicating that assisted migration may entail the risk of introducing biotic competition or interbreeding. Thus, rugged topography and dispersal barriers between islands are likely to amplify the future impacts of climate change in Japan. Limited areas in the central mountain region were identified as potential bioclimatic refugia, which should be conserved as a priority.  相似文献   

Changes in fungal community structure in bulk soil and spinach rhizosphere soil after chemical fumigation as revealed by 18S rDNA PCR-DGGE   总被引：3，自引：0，他引：3  

Yuko Takada HOSHINO  Naoyuki MATSUMOTO 《Soil Science and Plant Nutrition》2007,53(1):40-55

  相似文献   

Inactivation of staphylococcal enterotoxin-A with an electrolyzed anodic solution.   总被引：5，自引：0，他引：5  

Tetsuya Suzuki  Jun Itakura  Masumi Watanabe  Mari Ohta  Yuri Sato  Yuko Yamaya 《Journal of agricultural and food chemistry》2002,50(1):230-234

Electrolyzed anodic NaCl solutions [EW+], prepared by the electrolysis of 0.1% NaCl, have been shown to instantly inactivate most pathogens that cause food-borne disease. Elimination of food-borne pathogens does not necessarily guarantee food safety because enterotoxins produced by pathogens may remain active. We have tested whether EW+ can inactivate Staphylococcal enterotoxin A (SEA), one of the major enterotoxins responsible for food poisoning. Fixed quantities of SEA were mixed with increasing molar ratios of EW+, and SEA was evaluated by reversed-phase passive latex agglutination (RPLA) test, immunoassay, native polyacrylamide gel electrophoresis (PAGE), and amino acid analysis after 30 min incubations. Exposure of 70 ng, or 2.6 pmol, of SEA in 25 microL of PBS to a 10-fold volume of EW+, or ca. 64.6 x 10(3)-fold molar excess of HOCl in EW+, caused a loss of immuno-reactivity between SEA and a specific anti-SEA antibody. Native PAGE indicated that EW+ caused fragmentation of SEA, and amino acid analysis indicated a loss in amino acid content, in particular Met, Tyr, Ile, Asn, and Asp. Staphylococcal enterotoxin-A excreted into culture broth was also inactivated by exposure to an excess molar ratio of EW+. Thus, EW+ may be a useful management tool to ensure food hygiene by food processing industries.  相似文献   

Cartilage acidic protein-1B (LOTUS), an endogenous Nogo receptor antagonist for axon tract formation     

Sato Y  Iketani M  Kurihara Y  Yamaguchi M  Yamashita N  Nakamura F  Arie Y  Kawasaki T  Hirata T  Abe T  Kiyonari H  Strittmatter SM  Goshima Y  Takei K 《Science (New York, N.Y.)》2011,333(6043):769-773

Neural circuitry formation depends on the molecular control of axonal projection during development. By screening with fluorophore-assisted light inactivation in the developing mouse brain, we identified cartilage acidic protein-1B as a key molecule for lateral olfactory tract (LOT) formation and named it LOT usher substance (LOTUS). We further identified Nogo receptor-1 (NgR1) as a LOTUS-binding protein. NgR1 is a receptor of myelin-derived axon growth inhibitors, such as Nogo, which prevent neural regeneration in the adult. LOTUS suppressed Nogo-NgR1 binding and Nogo-induced growth cone collapse. A defasciculated LOT was present in lotus-deficient mice but not in mice lacking both lotus- and ngr1. These findings suggest that endogenous antagonism of NgR1 by LOTUS is crucial for normal LOT formation.  相似文献