First Identification of 12β-Deoxygonyautoxin 5 (12α-Gonyautoxinol 5) in the Cyanobacterium Dolichospermum circinale (TA04) and 12β-Deoxysaxitoxin (12α-Saxitoxinol) in D. circinale (TA04) and the Dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC)     

Michiru Akamatsu  Ryosuke Hirozumi  Yuko Cho  Yuta Kudo  Keiichi Konoki  Yasukatsu Oshima  Mari Yotsu-Yamashita 《Marine drugs》2022,20(3)

Saxitoxin and its analogues, paralytic shellfish toxins (PSTs), are potent and specific voltage-gated sodium channel blockers. These toxins are produced by some species of freshwater cyanobacteria and marine dinoflagellates. We previously identified several biosynthetic intermediates of PSTs, as well as new analogues, from such organisms and proposed the biosynthetic and metabolic pathways of PSTs. In this study, 12β-deoxygonyautoxin 5 (12α-gonyautoxinol 5 = gonyautoxin 5-12(R)-ol) was identified in the freshwater cyanobacterium, Dolichospermum circinale (TA04), and 12β-deoxysaxitoxin (12α-saxitoxinol = saxitoxin-12(R)-ol) was identified in the same cyanobacterium and in the marine dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC) for the first time from natural sources. The authentic standards of these compounds and 12α-deoxygonyautoxin 5 (12β-gonyautoxinol 5 = gonyautoxin 5-12(S)-ol) were prepared by chemical derivatization from the major PSTs, C1/C2, produced in D. circinale (TA04). These standards were used to identify the deoxy analogues by comparing the retention times and MS/MS spectra using high-resolution LC-MS/MS. Biosynthetic or metabolic pathways for these analogues have also been proposed based on their structures. The identification of these compounds supports the α-oriented stereoselective oxidation at C12 in the biosynthetic pathway towards PSTs.  相似文献   

Susceptibility of Djungarian hamsters (Phodopus sungorus) to Neospora caninum infection     

Uchida Y  Ike K  Kurotaki T  Takeshi M  Imai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):401-403

Djungarian hamsters were examined for the susceptibility to Neospora caninum infection. After 29 Djungarian hamsters were intraperitoneally inoculated with 5 x 10(6) N. caninum tachyzoites of JPA1 strain, some animals showed symptoms such as ataxia, and many tissue cysts were detected in the brain and a cyst in the muscular tunics of stomach. Especially, more than 100 cysts per head were observed after 5 weeks post inoculation. It is suggested that the Djungarian hamster is a model useful to examine neosporosis.  相似文献   

Lectin-binding characteristics and capacitation of canine epididymal spermatozoa   总被引：2，自引：0，他引：2  

Kawakami E  Morita Y  Hori T  Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(7):543-549

Cross sections of the testes and the caput, corpus and cauda epididymides removed from 12 dogs were stamped on glass slides, and the sperm on the slides were stained with 6 different FITC-lectins (Con A, DBA, PNA, PSA, SBA, and WGA) to examine the characteristics of the surface glycoproteins (GPs) on canine epididymal sperm. The corpus epididymal sperm were washed three times by centrifugation, and their lectin-binding characteristics were investigated. The washed sperm from the corpus and cauda epididymides were incubated for 24 hr, and the fertilizing capacity of the sperm was evaluated by calculating the percentages of actively motile sperm (%MO), hyperactivated sperm (%HA), and acrosome-reacted sperm (%AR), and the number of canine zona-pellucida (ZP)-binding sperm. The testicular sperm did not stain with SBA lectin, but the SBA lectin fluorescence was observed on the surface of the entire heads of the caput epididymal sperm. Although all of the entire heads or acrosomal regions of the corpus epididymal sperm stained with all 6 FITC-lectins, the heads and acrosomal regions of the cauda epididymal sperm did not stain with DBA or SBA lectins. Washing the sperm from the corpus epididymis resulted in loss of the fluorescence of the FITC-DBA and -SBA lectins. The mean %MO, %HA, %AR, and ZP-binding number of the cauda epididymal sperm after 24 hr of incubation were higher than the values for the corpus epididymal sperm. All of the mean values for the washed sperm from the corpus and cauda epididymides were higher than the values for the unwashed sperm from the corpus and cauda, and with the exception of %AR, the values from the washed sperm from the corpus epididymis were significantly higher (P<0.05, 0.01). The results indicate that DBA- and SBA-lectin-binding GPs on the surface of canine epididymal sperm are associated with the fertilizing capacity and may be decapacitation factors.  相似文献   

Cycas necrotic stunt virus isolated from gladiolus plants in Japan     

Kaoru Hanada  Fumiyoshi Fukumoto  Manabu Kusunoki  Mitsuro Kameya-Iwaki  Yuko Tanaka  Toru Iwanami 《Journal of General Plant Pathology》2006,72(6):383-386

An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV. The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656.  相似文献   

Adrenocorticotropic hormone-induced secretion of cortisol in goats is inhibited by androgen     

Yuko MAEJIMA  Masato AOYAMA  Noriko KOBAYASHI  Shoei SUGITA 《Animal Science Journal》2006,77(1):87-94

In a previous study, it was found that there are sex differences in goats with respect to the levels of cortisol secretion induced by transportation stress. We also found that treatment of castrated male goats with dihydrotestosterone (DHT) suppressed the increase in plasma cortisol concentration following transportation, but did not suppress the secretion of adrenocorticotropic hormone (ACTH). This suggests that androgen might block ACTH ‐ induced cortisol secretion. In order to examine this hypothesis, the effects of androgen on ACTH‐induced cortisol secretion in goats were investigated. First, castrated male goats were treated with testosterone (T), DHT or cholesterol (cho) for 21–25 days. Cho was used as a control for T and DHT treatment. Then, plasma cortisol concentrations were compared among the hormonal treatments after ACTH injection. Subsequently, the distribution of androgen receptors in the caprine adrenal gland was investigated. There were no differences in the basal cortisol concentrations among the hormonal treatments. However, plasma cortisol concentrations after ACTH injection were significantly lower in T ‐ and DHT ‐ treated goats than in cho ‐ treated goats. Androgen receptors were present in 60% of the cells in the zonae fasciculata and reticularis of the adrenal cortex, the regions that secrete glucocorticoids. These results suggest that androgen may act directly on the adrenal cortex to suppress cortisol secretion induced by ACTH.  相似文献   

Cloning of DNA fragments specific to the pathotype and race of <Emphasis Type="Italic">Verticillium dahliae</Emphasis>     

Toshiyuki Usami  Shu Ishigaki  Hiroko Takashina  Yuko Matsubara  Yoshimiki Amemiya 《Journal of General Plant Pathology》2007,73(2):89-95

Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction (PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race 1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB095266.  相似文献   

Assessment of wood surface roughness: comparison of tactile roughness and three-dimensional parameters derived using a robust Gaussian regression filter   总被引：1，自引：0，他引：1  

Yuko?FujiwaraEmail author  Yoshihisa?Fujii  Yutaka?Sawada  Shogo?Okumura 《Journal of Wood Science》2004,50(1):35-40

Japanese oak and Japanese beech were sanded by hand with abrasive papers of varying grit number. Two three-dimensional parameters selected to characterize their surface roughness – one parameter for the distribution of roughness-profile peaks and the other for the relative area of the roughness-profile peaks above the threshold height – were compared against tactile roughness. The parameters were obtained from roughness profiles as determined by a robust Gaussian regression filter (RGRF) using seven cutoffs. The RGRF filtering process was adjusted specifically for the evaluation of wood surface roughness. Except for a cutoff wavelength of 0.25mm, the RGRF lent itself well to the determination of roughness profiles. No distortion of roughness profiles occurred around deep valleys, and there was a good correlation between the parameters and tactile roughness.Part of this study was presented at the 2002 Kansai Branch Office lecture meeting of the Japan Society for Precision Engineering, Kyotanabe, August 2002  相似文献   

Atmospheric lead and cadmium deposition within forests in the Kanto district, Japan     

Yuko Itoh  Satoru Miura  Shuichiro Yoshinaga 《Journal of Forest Research》2006,11(2):137-142

Atmospheric lead and cadmium deposition in bulk precipitation and throughfall was investigated at four forests in the Kanto district, Japan, to assess the impact of human activities on the environmental health of forests. Annual lead and cadmium depositions in bulk precipitation ranged from 8.9 to 25.7 g ha⁻¹ year⁻¹ and from 0.77 to 1.30 g ha⁻¹ year⁻¹, respectively. Lead and cadmium deposition increased in the summer at every forest due to large amounts of rainfall. At one of the forests, the depositions were also high in the winter due to heavy snowfall. These depositions were similar to recent depositions observed at other rural and urban sites in Japan and several forests in Europe and North America after 1990. These results indicate that although anthropogenic lead and cadmium are deposited at these rates over wide areas, depositions are still higher than in remote areas.  相似文献   

First report of trunk rot caused by <Emphasis Type="Italic">Fomitiporia torreyae</Emphasis> in Kyoto prefecture on cultivars of Japanese cedar with no relatedness to ‘Sanbu-sugi’     

Yuko Ota  Megumi K. Kimura  Tsutomu Hattori  Yurika Miyuki  Ryota Endo 《Journal of Forest Research》2016,21(2):105-109

Trunk rot, caused by Fomitiporia torreyae, is one of the most economically important sap rot diseases on Cryptomeria japonica, especially on a cutting cultivar ‘Sanbu-sugi’. This disease had been reported only from Chiba and Ibaraki prefectures in Japan; however, a similar trunk rot on Japanese cedars was found recently in Kyoto prefecture. We identified the causal agent of the trunk rot on Japanese cedars in Kyoto prefecture, clarified the genotype of ‘Sanbu-sugi’ (sensu stricto) using microsatellite markers, and then investigated the relatedness of these infected cedars with ‘Sanbu-sugi’. The pathogen was identified as F. torreyae by its DNA sequences and morphological characteristics of the fruiting body. The 25 ramets of ‘Sanbu-sugi’ had only one genotype based on eight microsatellite loci. Infected Japanese cedars in Kyoto were comprised of different genotypes and showed no close relatedness to ‘Sanbu-sugi’. This is the first report of trunk rot caused by F. torreyae on cultivars of Japanese cedar with no relatedness to ‘Sanbu-sugi’ outside of Chiba and Ibaraki prefectures and showed the potential for expansion of this disease to Japanese cedar plantations in other areas.  相似文献   

Temporal and spatial dynamics of an old-growth beech forest in western Japan     

Uyanga Ariya  Ken-Ya Hamano  Takushi Makimoto  Shu Kinoshita  Yasuaki Akaji  Yuko Miyazaki  Muneto Hirobe  Keiji Sakamoto 《Journal of Forest Research》2016,21(2):73-83

Dendrochronological approaches enable us to understand forest stand dynamics by estimation of disturbance history and age structure. The present study was conducted in an old-growth beech forest in a forest reserve in western Japan. Increment cores were taken for tree ring analysis from all canopy trees in a 50 m × 130 m study plot. Radial growth release criteria were developed to identify significant growth releases in each tree ring series and to characterize the disturbance history of the study site. The age structure of the forest was indicative of continuous establishment by Fagus crenata and simultaneous establishment by Magnolia obovata. A variety of low-intensity disturbances were identified in each decade, especially after the 1900s, but the occurrence of high-intensity catastrophic disturbance was rare, and likely played an important role in maintaining species diversity in the existing forest canopy. The results also suggest that F. crenata regenerates gradually before and after both large- and small-scale disturbances, whereas M. obovata and Betula grossa regenerate only after large-scale catastrophic disturbances.  相似文献