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排序方式: 共有217条查询结果,搜索用时 31 毫秒
71.
Distinct regional profiles of trace element content in muscle of Japanese eel Anguilla japonica from Japan, Taiwan, and China 总被引:1,自引:0,他引:1
Trace element distribution patterns were examined in Japanese eel from five Asian regions for the purpose of identifying the
elements and patterns that could be used to determine provenance. Distinctive patterns were found in eels from the Japanese
prefectures of Miyazaki, Kagawa, and Shizuoka, and from Taiwan and China. The levels of six trace elements, selenium, mercury,
copper, manganese, zinc, and arsenic, in muscle tissue varied among eels of different origins, and factorial analysis of the
elemental patterns showed that Factor 1 was attributable to selenium and mercury levels and Factor 2 to zinc and copper levels.
Multivariate analysis showed that the patterns of elemental composition in fish muscle were mainly differentiated by these
two factors. 相似文献
72.
Takehiko?Yokoyama Yumiko?Tanaka Minoru?Sato Nobuhiro?Kan-No Toshiki?Nakano Toshiyasu?Yamaguchi Eizoh?NagahisaEmail author 《Fisheries Science》2005,71(4):924-930
ABSTRACT: In this paper, the authors report the detection of alanine racemase activity in the marine diatom Thalassiosira sp. Since the Thalassiosira sp. was cultured under germ-free conditions, it appeared that D-alanine was not derived from bacteria but was produced through catalysis by algal alanine racemase. The rate of conversion of L-alanine to D-alanine was approximately the same as that for the reverse reaction, and the enzyme catalyzed the equilibration of the D- and L-forms. The crude enzyme preparation obtained from the cells at the stationary phase of the growth cycle had an optimal pH of approximately 9.5. The Lineweaver–Burk analysis showed that the K m for D- and L-alanine was 16.5 mM and 29.4 mM, respectively. It appears that the enzyme is highly specific for D- or L-alanine because it does not catalyze the racemization of other amino acids. In addition, after gel filtration, the enzyme did not require exogenous pyridoxal 5'-phosphate (PLP) for its activity, however, the effects of several chemicals suggest that the enzyme may be PLP-dependent. The enzyme is more similar to that found in invertebrates when compared with that found in bacteria. This is the first report on the occurrence of alanine racemase activity in the microalga Thalassiosira sp. 相似文献
73.
ABSTRACT: The distribution of mercury in the muscle and other tissues was determined in 23 species of fishes and invertebrates. This study reveals species-specific patterns of mercury accumulation in the muscle of tuna and alfonsino. Consistently high levels of total mercury were found in Beryx splendens (0.78 ± 0.56 µg/g), Atlantic Thunnus thynnus (0.42 ± 0.06 µg/g), Pacific T. thynnus (0.59 ± 0.34 µg/g), Thunnus obesus (0.98 ± 0.34 µg/g), Makaira nigricans (0.56 ± 0.05 µg/g), Tetraptrus audax (0.51 ± 0.08 µg/g), and Xiphias gladius (0.47 ± 0.24 µg/g). Other fish species had lower levels of total mercury or methylmercury in muscle than the maximum permitted level of mercury in fish in Japan (0.4 µg/g). Total mercury and methylmercury levels in the muscles of T. thynnus , T. obesus, and B. splendens were closely correlated with body weight. 相似文献
74.
Makoto Ueno Yumiko Kumura Kana Ueda Junichi Kihara Sakae Arase 《Journal of General Plant Pathology》2011,77(3):209-213
In leaves pretreated with an indole derivative [indole-3-acetic acid (IAA) tryptamine, or tryptophan], blast lesion formation
was suppressed compared to those treated with distilled water (DW) as a control. Phenylalanine ammonia-lyase (PAL) activity
and PAL expression were significantly enhanced in the IAA- or tryptophan-pretreated leaves, but not in tryptamine-pretreated leaves.
This induction of resistance was inhibited by pretreatment with a PAL inhibitor, α-aminooxyacetic acid, in IAA- and in tryptophan-treated
leaves, but not in tryptamine-treated leaves. This study strongly shows that the indole derivatives IAA tryptamine and tryptophan
can enhance a disease resistance mechanism that is supported by different metabolic pathways. 相似文献
75.
Sakuma M Nishio T Nakanishi N Izawa M Asari Y Okamura M Shimokawa Miyama T Setoguchi A Endo Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1381-1384
We herein present clinical findings of an Iriomote cat with Hepatozoon felis parasitemia. A male Iriomote cat was captured for ecological analyses three times from January 2010 to January 2011. Although this cat did not show any hematological abnormalities at the time of the first capture, H. felis parasitemia and increased serum creatine kinase levels were detected at the second and third captures. H. felis infection was confirmed by polymerase chain reaction, and amplified 18S ribosomal RNA gene fragments were 100% identical to those of H. felis in leopard cats in Korea. Although the virulence of H. felis in this cat was suggested to be low, this is the first report of an H. felis-infected Iriomote cat with parasitemia. 相似文献
76.
Kagawa Y Yamashita T Maetani S Aoki Y Sakaguchi K Hirayama K Umemura T Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1221-1224
A lymphoplasmacytic lymphoma was diagnosed in a 12- year-old domestic cat that had a primary cutaneous mass involving the stomach, liver, kidneys, heart, abdominal wall, diaphragm, bone marrow and several lymph nodes. Histopathologically, the most characteristic feature of this tumor was the heterogeneity of cell components, such as small lymphocytes, well-differentiated plasma cells and plasmacytoid transformed lymphocytes. Amyloid was deposited in the skin, stomach, and several lymph nodes. Immunohistochemically, neoplastic small lymphocytes were positive for CD20, and well-differentiated plasma cells and plasmacytoid transformed lymphocytes were positive for λ-Ig light chains and MUM1/IRF-4. These results emphasize the importance of lymphoplasmacytic lymphoma as a differential diagnosis of extramedullary cutaneous plasmacytoma in cats. 相似文献
77.
Shimoyama Y Kirat D Akihara Y Kawasako K Komine M Hirayama K Matsuda K Okamoto M Iwano H Kato S Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):599-604
In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine. 相似文献
78.
Rieko OKAME Keiko NAKAHARA Yumiko KATO Makoto BANNAI Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):661-668
It has been suspected that in comparison with glucose or fatty acids, the levels of amino
acids may readily change with different forms of exercise. In the present study, we
measured the concentrations of amino acids, glucose, triglycerides, total protein and
total cholesterol in the blood and/or cerebrospinal fluid (CSF) of rats subjected to
forced running exercise on a treadmill, and voluntary running exercise using a wheel, with
a constant running distance of 440 m. Rats that performed no running and rats subjected to
immobilization stress were used as controls. We observed a few significant changes in the
levels of plasma glucose, triglycerides, total protein and total cholesterol in all
groups. Whereas, plasma amino acid levels were significantly changed by exercise and
stress, especially during the light period. The plasma levels of many amino acids were
specifically increased by forced running; some were decreased by immobilization stress.
Few amino acids showed similar changes in their levels as a result of voluntary running.
In addition, there was a significant difference in the degree of amino acid imbalance
between blood and CSF. These results provide the first information on changes in levels of
amino acids in plasma and CSF resulting from forced and voluntary exercises. 相似文献
79.
Mai YAMAMOTO Takashige KASHIMOTO Ping TONG Jianbo XIAO Michiko SUGIYAMA Miyuki INOUE Rie MATSUNAGA Kohei HOSOHARA Kazue NAKATA Kenji YOKOTA Keiji OGUMA Koichiro YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):823-828
Vibrio vulnificus is the causative agent of primary septicemia, wound
infection and gastroenteritis in immunocompromised people. In this study, signature-tagged
mutagenesis (STM) was applied to identify the virulence genes of V.
vulnificus. Using STM, 6,480 mutants in total were constructed and divided into
81 sets (INPUT pools); each mutant in a set was assigned a different tag. Each INPUT pool
was intraperitoneally injected into iron-overloaded mice, and in vivo
surviving mutants were collected from blood samples from the heart (OUTPUT pools). From
the genomic DNA of mixed INPUT or OUTPUT pools, digoxigenin-labeled DNA probes against the
tagged region were prepared and used for dot hybridization. Thirty tentatively attenuated
mutants, which were hybridized clearly with INPUT probes but barely with OUTPUT probes,
were negatively selected. Lethal doses of 11 of the 30 mutants were reduced to more than
1/100; of these, the lethal doses of 2 were reduced to as low as 1/100,000.
Transposon-inserted genes in the 11 attenuated mutants were those for IMP dehydrogenase,
UDP-N-acetylglucosamine-2-epimerase, aspartokinase, phosphoribosylformylglycinamidine
cyclo-ligase, malate Na (+) symporter and hypothetical protein. When mice were immunized
with an attenuated mutant strain into which IMP dehydrogenase had been inserted with a
transposon, they were protected against V. vulnificus infection. In this
study, we demonstrated that the STM method can be used to search for the virulence genes
of V. vulnificus. 相似文献
80.
It should be emphasized that “129” is not simply a number but is also the designation of a mouse strain that
has made a great contribution to modern biological science and technology. Embryonic stem cells derived from
129 mice were essential components of gene-targeting strategies in early research. More recently, 129 mice
have provided superior donor genomes for cloning by nuclear transfer. Some factor or factors conferring
genomic plasticity must exist in the 129 genome, but these remain unidentified. 相似文献