Effect of administration of acid‐ and bile‐tolerant lactobacilli on pigs intestinal microbial population     

Makoto OTSUKA  Tomoko MUTO  Yu KATSUNUMA  Yutaka NAKAMURA  Ryozo TAKADA  Hajime MINATO 《Animal Science Journal》2002,73(6):497-503

The effects of the administration of four Lactobacillus strains as probiotics on the constitution of microbial populations in the intestine of pigs were investigated by using a litter of pigs. The experimental group was fed a diet blended with the freeze‐dried powder containing lactobacilli at a concentration of 0.2% (W/W). After 4 weeks of probiotics administration, the total viable count of anaerobes in the feces was not significantly different between the pigs fed a diet supplemented with probiotics and the pigs given the non‐supplemented diet. However, viable counts of eubacteria were significantly higher (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than in the pigs given the non‐supplemented diet. Conversely, viable counts of clostridia were significantly lower (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than the pigs given the non‐supplemented diet. The total volatile fatty acid concentrations in the feces of pigs that were given a diet supplemented with probiotics were significantly lower (P < 0.05) than those given the non‐supplemented diet. Further, the molar proportion of n‐butyric acids in the feces of pigs that were given a diet supplemented with probiotics, was significantly lower (P < 0.05) than those given the non‐supplemented diet.  相似文献   

The effects of medetomidine and xylazine on gastrointestinal motility and gastrin release in the dog     

K. NAKAMURA  S. HARA  & N. TOMIZAWA 《Journal of veterinary pharmacology and therapeutics》1997,20(4):290-295

The effect of medetomidine, a potent and highly selective α₂-adrenoceptor agonist, on the motility of the gastric antrum, duodenum, mid-jejunum and ileum was investigated in ten dogs. Its effect on the release of gastrin was also determined. Administration of medetomidine intramuscularly (i.m.) at a dose of 40 μg/kg inhibited the motility of the gastric antrum, duodenum, mid-jejunum and ileum significantly, in comparison to administration of xylazine intramuscularly at a dose of 2.0 mg/kg. The release of gastrin was also significantly decreased in dogs receiving medetomidine. It was found to inhibit the motility in the gastric antrum and duodenum longer than in the mid-jejunum and ileum, presumably by acting specifically on α₂-adrenoceptors, likely at the peripheral level. Medetomidine also inhibited the gastric contraction associated with gastrin secretion.  相似文献   

In vitro effects of insulin on glucose and lipid metabolism in rat embryos   总被引：1，自引：0，他引：1  

Hirotada TSUJII  Yumi NAKAMURA and  Koh-ichi HAMANO 《Animal Science Journal》2002,73(3):185-189

Glucose is utilized for oxidation and synthesis of various lipids in cultured rat embryos. The present experiment examined the effect of insulin on the incorporation of glucose into lipid fractions in rat embryos in vitro . Embryos at the 2-cell, 8-cell and blastocyst stages were incubated for 5 h in hamster embryo culture medium (HECM)-1 containing ¹⁴C-glucose and 170 nmol/L insulin, or in HECM-1 containing only ¹⁴C-glucose, and the oxidation of glucose in these embryos was examined. In addition, the total lipids of blastocysts were separated by thin layer chromatography and the radioactivity of the separated lipid fractions was measured. Oxidation of glucose was significantly increased after insulin treatment compared with that without insulin treatment in 8-cell embryos and blastocysts ( P  < 0.05), but not in 2-cell embryos. Incorporation of glucose into lipids in blastocysts was significantly lowered by insulin treatment compared with that without insulin treatment ( P  < 0.05). Most of the radioactivity was recovered from triacylglycerols of blastocysts and the remaining radioactivity was found in other neutral lipids and phospholipids. We conclude that insulin accelerates the utilization for oxidation of glucose and inhibits the storage of triacylglycerols in rat blastocysts.  相似文献   

Computed tomographic images of discospondylitis in a calf     

Takeshi TSUKA  Naoki YAMAMOTO  Makoto SANESHIGE  Takehito MORITA  Yuji SUNDEN  Yusuke MURAHATA  Kazuo AZUMA  Tomohiro OSAKI  Norihito ITO  Yoshiharu OKAMOTO  Tomohiro IMAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1689-1691

A 2-month-old male Japanese Black calf was presented with a 30-day history of progressive ataxia. Antemortem examination using computed tomography (CT) revealed narrowing of the disc spaces due to destruction of intervertebral structures between the first and second thoracic vertebrae and between the second and third thoracic vertebrae. Osteolysis was evident as irregular hypoattenuating lesions within the opposing end plates of the first, second and third thoracic vertebrae. Pseudomonas aeruginosa was detected as the causative bacteria, and discospondylitis was diagnosed. To the best of our knowledge, this is the first bovine case report describing the application of CT for the diagnosis of discospondylitis.  相似文献   

Impact of farmland reclamation on soil distribution in Japan: the case of Andosols in Nagano Prefecture     

Kida  Kimihiro  Ibori  Yusuke  Kawahigashi  Masayuki 《Journal of Soils and Sediments》2021,21(5):1938-1946

Journal of Soils and Sediments - In modern times, farmlands in Japan have been reconstructed at a large-scale through the cutting and filling of soils. In principle, such reconstruction should be...  相似文献   

利用聚合酶链式反应技术研究单粒古代稻谷(公元800年)离体DNA片段的扩增     

中村郁郎  佐滕洋一郎 《中国水稻科学》1991,(4)

在东南亚地区已发掘出很多古代稻谷,如果能从这些古稻谷中把DNA提出加以分析,可以得到有关栽培稻的系统分化和地理传播方面的直接信息。古代稻种的基因型相当复杂,必须要能从单粒种子开始分析,方能得到有价值的资料。为此,我们进行了从单粒古稻谷中提取DNA的研究工作。本研究采用通常提取植物组织中DNA的方法。从在日本挖掘出的古代稻谷单粒种子中提取出了50—100ng左右的DNA片段。以这些DNA作为模板,用聚合酶链式反应(Polymerase Chain Reaction,PCR)技术将DNA加以扩增。由其中几个DNA片段初步合成了相当于水稻光敏色素基因的DNA序列。  相似文献   

Glutathione levels in phloem sap of rice plants under sulfur deficient conditions     

Yusuke Kuzuhara  Amiko Isobe  Motoko Awazuhara  Toru Fujiwara  Hiroaki Hayashi 《Soil Science and Plant Nutrition》2013,59(1):265-270

Glutathione which is an abundant reduced sulfur compound in plants is considered to play important roles in the transmission of the sulfur nutrient status between organs within the plant body and in the long-distance transport of reduced sulfur. We determined the concentrations of glutathione, γ-glutamylcysteine (γ-EC), sulfate in the rice phloem sap collected by the insect laser technique. Phloem sap was collected from the plants cultured in sulfur-deficient and control solutions. The concentration of glutathione in the rice phloem sap was higher than that of sulfate in both control and sulfur-deficient plants. Under sulfur-deficient condition, the concentration of glutathione in the phloem sap did not decrease, whereas the sulfate concentration decreased significantly. The pattern of changes in γ-EC concentration was similar to that of glutathione. These data indicate the presence of mechanisms for the maintenance of a constant glutathione concentration in the phloem sap in rice plants under sulfur deficiency, whereas the sulfate concentration was found to be relatively unstable.  相似文献   

Delineation of Japanese soil temperature regime map     

Yusuke Takata  Tsuneo Kuwagata  Kazunori Kohyama  Hiroshi Obara 《Soil Science and Plant Nutrition》2013,59(2):294-302

The soil temperature regime map provides for utilitarian classification that can be superimposed on soil classification to permit more precise interpretations and assessments of land use. The objects of this study are (1) to clarify the relationship between soil temperature and meteo-geographical factors, and then (2) to delineate detailed soil temperature regime map (1?km grid) as Japanese land resources inventory. There was a parallel relationship between mean annual soil temperature (MAST) and mean annual air temperature (MAAT), but this relationship was affected to some extent by the mean annual wind speed and mean annual global irradiation in this study. Furthermore, the difference between MAST and MAAT [Diff(MAST–MAAT)] showed the highest correlation with elevation. The map of RK_Diff(MAST–MAAT) was computed using this meteo-geographical relationship with the regression-kriging approach, and then the map of MAST and the soil temperature regime map were delineated using the map of MAAT and the RK_Diff(MAST–MAAT). The root mean square error of this delineation procedure was 0.47°C. It was clear that the majority of the Japanese soils had “mesic” soil temperature regime, and Japanese agricultural land was mainly distributed at “mesic” area and followed by “thermic”, “frigid”, and “hyperthermic” area. For promoting this land resource inventory, the soil temperature regime map will be uploaded on “Soil Information Web Viewer (http://agrimesh.dc.affrc.go.jp/soil_db/)”, which is provided by the National Institute for Agro-Environmental Sciences.  相似文献   

Comparative studies of the changes in enzymatic activities in hardy and less hardy cultivars of winter wheat in late fall and in winter under snow     

Shonosuke Sagisaka  Yusuke Matsuda  Tohru Okuda  Sachio Ozeki 《Soil Science and Plant Nutrition》2013,59(3):543-550

Abstract

The synthetic activity of protein and changes in the activity profiles of enzymes during cold acclimation were studied in leaves, stems, and crowns of a hardy winter wheat cultivar ( Triticum aestivum L. cv. Horoshirikomugi) and a less hardy winter wheat cultivar (Triticum aestivum L. cv. Norin 61). The results showed that one of the genetic differences between the two cuitivars is found in the activities of protein synthesis under low temperatures. The profiles of the changes in enzymatic activities showed that the adjustment of enzymatic activities was a tissue-specific event to accommodate environmental changes. The synthesis of the enzymes involved in a peroxide scavenging system such as hexokinase, glucose-6-phosphate dehydrogenase, dehydroascorbate reductase, ascorbate peroxidase, and so on started at the acclimation stage, while the phosphoglucose isomerase, which appears to be a maintenance-type enzyme in the wintering wheat, showed a small increase in the activity at later stages of acclimation period in their stems of both cuitivars. When the field was covered with snow, the catalase activity in the leaves started to decrease abruptly, while catalase activities in the stems and crowns did not exhibit appreciable changes during the life under snow. The life-cycle dependent changes in enzymatic activities appear to occur in late March or early April under snow.  相似文献   

Comparison of acute inhalation toxicity of sulfuric acid by the inhalation and intratracheal instillation methods     

Kazuya Takeuchi  Yusuke Kuroda  Takamasa Numano  Masayuki Kimura  Seigo Hayashi  Satoshi Furukawa 《Journal of toxicologic pathology》2021,34(3):269

Recently, intratracheal instillation has been focused on as a simple, low-cost alternative to the inhalation method. In this study, intratracheal instillation of sulfuric acid, a typical acidic compound, was performed to compare the acute toxicity of acidic compounds that could cause damage to the respiratory system between intratracheal instillation and inhalation. Sulfuric acid was administered to male rats at doses of 0.7, 2, 7, 20, and 60 mg/kg by dividing the total dose into four doses. General condition and body weight were examined up to 14 days after administration, and macropathological and histopathological examinations were performed. The half-lethal dose was then estimated. All animals administered 20 and 60 mg/kg sulfuric acid and one animal administered 2 mg/kg sulfuric acid died within 4 h after administration. No abnormalities were observed in other animals. At 20 and 60 mg/kg, multiple red foci or diffuse red areas were macroscopically observed in the lungs. In these lesions, histopathologically, clefts between the mucosal epithelium and basement membrane and necrosis of the alveolar epithelium were observed. Deaths in these groups may have resulted from lung injury. No notable changes were observed in other animals. Therefore, the half-lethal dose of sulfuric acid by intratracheal instillation was estimated as 7–20 mg/kg. The acute toxicity by intratracheal instillation was evaluated with two-fold sensitivity since the exposure at the half-lethal sulfuric acid concentration in inhalation studies was calculated as 43.2 mg/kg.  相似文献