Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method     

Noboru SASAKI  Yoshinori IKENAKA  Yumiko INOUE  Takahiro ICHISE  Noriyuki NAGATA  Mayumi ISHIZUKA  Syouta MM NAKAYAMA  Kensuke NAKAMURA  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):648

Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5–10 µg/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144–47,961) was significantly higher (P<0.05) than that of 13 dogs with hypercortisolism (125, range 52–224) or 15 healthy dogs (85, range 50–117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.  相似文献   

Autologous tunica vaginalis communis flap for repairing an abdominal wall hernia in a dog     

Yusuke Ozai  Akiko Uemura  Ryou Tanaka  Seijirow Goya 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(4):393

An 8-year-old, intact male miniature dachshund dog, weighing 8.6 kg, was presented with a soft swelling in the caudal abdominal region, including both sides of the groin area. Laparotomy revealed a severe caudal abdominal wall hernia with atrophy of the rectus abdominal muscle. The defect was repaired using a tunica vaginalis communis flap following a standard open prescrotal castration. There were no complications or recurrence of the hernia at 11 months after surgery. This surgical technique involves autogenous reconstruction, is easy to perform, and requires minimal dissection. The tunica vaginalis communis flap has potential clinical applications for repairing caudal abdominal wall hernias in male dogs.  相似文献   

Clinical features and their course of pituitary carcinoma with distant metastasis in a dog     

Munekazu NAKAICHI  Toshie ISERI  Hiro HORIKIRIZONO  Yusuke SAKAI  Harumichi ITOH  Hiroshi SUNAHARA  Kazuhito ITAMOTO  Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1671

An 11-year-old male toy poodle with neurological symptoms was diagnosed with a macroscopic pituitary tumor, which produced adrenocorticotropic hormone. Radiation therapy with a linear accelerator was performed for the pituitary tumor, and resulted in good local tumor control. However, serum endogenous adrenocorticotropic hormone concentrations were uncontrollable even after the tumor disappeared. Abdominal computed tomography revealed splenic masses, and splenectomy was performed. Histopathological examination of the surgical specimen showed tumor cells with eosinophilic and finely granular cytoplasm suggestive of endocrine origin. Since these cells were positive for adrenocorticotropic hormone, the case was diagnosed as a pituitary carcinoma with distant metastasis. Necropsy revealed multiple metastases to the abdominal organs. This is the first case report describing canine pituitary carcinoma with distant metastasis.  相似文献   

Infection of tilapia, Oreochromis mossambicus (Trewavas), by a marine monogenean, Neobenedenia melleni (MacCallum, 1927) Yamaguti, 1963 in Kaneohe Bay, Hawaii, USA, and its treatment   总被引：2，自引：0，他引：2  

J. J. KANEKO II  R. YAMADA  J. A. BROCK  R. M. NAKAMURA 《Journal of fish diseases》1988,11(4):295-300

Abstract A disease of saltwater, cage-cultured tilapia, Oreochromis mossambicus (Trewavas), caused by the marine monogenean, Neobenedenia melleni (MacCallum, 1927) Yamaguti, 1963, is described. Up to 400 parasites were found attached to the body surface of individual fish. Heavily infected fish showed hyperirritability, heavy mucus secretion and discoloration. Pathology was most marked on the eye, with corneal opacity initially, followed by buphthalmos, corneal ulceration and rupture of the eye with subsequent degeneration of internal structure. The infection was successfully treated using 2 min freshwater dips.  相似文献   

基于PCA和聚类分析的茶树F1代茶多酚遗传分析   总被引：1，自引：0，他引：1  

李家华  张广辉  邵宛芳  坂田祐介  根角厚司  橘本文雄 《分子植物育种》2011,9(4):491-497

高效液相色谱检测茶树(Camellia sinensis (L.) O.Kuntze) 14个红富贵×红交27的F1代单株、7个红交27×F95181 F1代单株及其亲本中各儿茶素组分含量,利用主成分分析和聚类分析研究了茶多酚在品种间杂交F1代的遗传规律.结果显示,红富贵×红交27 F1代14个单株茶多酚的表型以3:1...  相似文献   

Photoinduced oxidation of the insecticide phenothrin on soil surfaces     

Suzuki Y  Lopez A  Ponte M  Fujisawa T  Ruzo LO  Katagi T 《Journal of agricultural and food chemistry》2011,59(18):10182-10190

Photodegradation profiles of the pyrethroid insecticide phenothrin on a moistened U.S. soil thin layer was investigated by using its predominant component, the 1R-trans-isomer (I), under continuous exposure to light at >290 nm from a xenon arc lamp. Its degradation was moderately accelerated by irradiation with half-lives of 5.7-5.9 days (dark control 21-24 days), mainly via successive oxidation of the 2-methylprop-1-enyl group and ester cleavage followed by mineralization to carbon dioxide. Spectroscopic and cochromatographic analyses showed that the major degradates were the alcohol and ketone derivatives of I formed via photoinduced oxidation of the 2-methylprop-1-enyl group by singlet oxygen. The photoinduced generation of singlet oxygen in/on the soil surface was confirmed by using chemical trapping reactions together with ESR spectroscopy.  相似文献   

Production of mouse fibroblast growth factor 4 in E. coli and its application for isolation and maintenance of mouse trophoblast stem cells in vitro     

Hosoi Y  Ando Y  Arakawa M  Kasuga K  Kojima I  Kobayashi M 《The Journal of reproduction and development》2011,57(5):650-654

Fibroblast growth factor 4 (FGF4) promotes isolation of trophoblast stem (TS) cells from mouse blastocysts and maintenance of TS cells in an undifferentiated state in vitro. To date, commercially available, bacterially expressed human FGF4 (RhFGF4) has been used generally for this purpose. In this study, HismFGF4, a 6x histidine-tagged mouse FGF4, was produced in E. coli and purified using heparin column chromatography. We demonstrated that HismFGF4 (25 ng/ml) more efficiently generates mouse TS cells from a single blastocyst than RhFGF4 (25 ng/ml) and that TS cells isolated and maintained with HismFGF4 retained their ability to differentiate into the trophoblast cell lineage in vitro. In addition, TS cells cultured with HismFGF4 (25 ng/ml) were maintained in an undifferentiated state better than with RhFGF4 (25 ng/ml). To the best of our knowledge, this is the first application of a mouse FGF4 derivative for isolation and maintenance of mouse TS cells.  相似文献   

Behavioral indexes of piglet welfare: Comparison of indoor and outdoor housing systems     

Kouzo NAKAMURA  Toshio TANAKA  Kouji NISHIDA  Katsuji UETAKE 《Animal Science Journal》2011,82(1):161-168

The purpose of this experiment was to establish behavioral indexes of piglet welfare. Forty‐eight piglets were allocated to either four indoor pens or four outdoor pens (six piglets per pen). The indoor system was a commercial pen that consisted of a concrete floor and a slat floor. The outdoor system had a dirt paddock with a wooden hutch. Growth performance, salivary cortisol levels, skin lesions and behaviors of the piglets were monitored for 4 weeks. Sixteen types of behaviors were recorded by using 2‐min instantaneous scan sampling for 8 h/day. Growth performance and salivary cortisol levels did not significantly differ between the two housing systems. On the other hand, skin lesions and behaviors were significantly affected by the housing system. The number of skin lesions was higher in the indoor system. In addition, piglets in the outdoor system showed more investigative and social‐play behaviors than those in the indoor system. Piglets in the indoor system showed more resting, drinking, moving, fighting, and conflict behaviors than those in the outdoor system. We conclude that investigative, social‐play and conflict behaviors may be effective indexes of the welfare level of piglets, especially investigative and conflict behaviors.  相似文献   

Effect of the expression level of an AGAMOUS-like gene on the petaloidy of stamens in the double-flowered lily, ‘Elodie’     

Yusuke Akita  Mutsumi Nakada  Akira Kanno 《Scientia Horticulturae》2011

Double-flowered lilies, in which stamens are converted to petaloid organs, are valuable for horticulturists. ‘Elodie’ is a double-flowered lily cultivar in which stamens are homeotically converted into petaloid organs in whorl 3. The ‘Elodie’ cultivar shows individual variation in stamen structure and it was therefore classified into the following three types according to the strength of petaloidy of the stamens: weak (type-I), intermediate (type-II), and strong (type-III) phenotypes. The AGAMOUS (AG) gene is a class C floral identity gene in Arabidopsis thaliana that is involved in the formation of stamens and carpels. An AG-like gene was isolated from ‘Elodie’ (LelAG1) and its expression was compared between flower types. The LelAG1 gene was expressed in whorls 3 and 4, but not in whorls 1 and 2 in all flower types. In type-I flowers, LelAG1 was expressed strongly in whorls 3 and 4, while its expression was significantly decreased in whorl 3 in type-III flowers. In type-II intermediate phenotype flowers, the expression level of LelAG1 in whorl 3 was reduced by 60%. These results suggest that the expression level of AG-like genes is correlated with the degree of petaloidy of the stamens.  相似文献   

Molecular cloning, nucleotide sequence and characteristics of a xylanase gene (xynA) from Ruminococcus albus 7     

Mutsumi NAKAMURA  Takafumi NAGAMINE  Akio TAKENAKA  Rustem I. AMINOV  Koretsugu OGATA  Kiyoshi TAJIMA  Hiroki MATSUI  Yoshimi BENNO  Hisao ITABASHI 《Animal Science Journal》2002,73(5):347-352

A 2.6-kb DNA fragment encoding a xylanase gene ( xyn A) was cloned from the rumen hemicellulolytic bacterium Ruminococcus albus 7. The deduced primary structure of the protein (XynA) was divided into a signal peptide region and 3 domains. Domain A was identified as a family 11 (G) catalytic domain, but one amino acid residue was replaced by another in an active site signature 1 of family 11. Domain B is a stabilizing domain for the catalytic domains of families 10 and 11. Deletion of domain B reduced stability of the xylanase at high temperature and at high and low pH. Domain B may be useful for protein engineering of xylanase. Domain C has sequence similarity to deacetylases and NodB proteins.  相似文献