Correlation between cytochrome P450 1A (cyp1a) mRNA expression and ambient phenanthrene and pyrene concentration in Javanese Medaka Oryzias javanicus     

Rusni  Suhaila  Sassa  Mieko  Takehana  Yusuke  Kinoshita  Masato  Inoue  Koji 《Fisheries Science》2020,86(4):605-613

Fisheries Science - Cytochrome P450 1A (Cyp1a) catabolizes polycyclic organic compounds. In this study, we sequenced the cyp1a gene from two Javanese medaka Oryzias...  相似文献   

我国草莓用农药登记现状分析     

姜莉莉  田中一久  卢小永  孙瑞红  武冲 《生物灾害科学》2021,44(2):159-162

[目的]我国草莓以设施栽培为主,病虫发生多且严重,施用农药是草莓生产的重要环节.为了给草莓病虫害科学防控及农药登记提供参考,对目前我国草莓用农药登记现状进行分析.[方法]根据中国农药信息网登记的草莓用农药信息,对登记农药的种类、数量、防治对象、剂型及毒性进行统计分析.[结果]结果表明,截至目前,我国草莓用农药登记产品仅...  相似文献   

Semipersistency of <Emphasis Type="Italic">Myzus persicae</Emphasis> Transmission of Cucumoviruses Systemically Infecting Leguminous Plants     

Mitsuro KAMEYA-IWAKI  Kimiaki MURAKAMI  Shin-ichi ITO  Kaoru HANADA  Shuhei TANAKA 《Journal of General Plant Pathology》2000,66(1):64-67

Sequential transmission tests of Peanut stunt virus (PSV) and Cucumber mosaic virus (CMV) systemically infecting common bean, Phaseolus vulgaris, were conducted using Myzus persicae allowed to fast for 2 hr and then to acquisition feed on infected common bean plants or purified virus for 10 min. In the sequential transmission tests using either one or 10 aphids per assay plant, three isolates of PSV (J,S,Y5) and one of CMV (V) were transmitted from and to common bean up to a third or fourth inoculation access. Many aphids transmitted these viruses to two or three plants. Purified viruses of PSV-S and CMV-V were also transmitted up to a third or second inoculation access at low percentage. On tobacco, Nicotiana tabacum, aphids transmitted PSV-S and CMV-V only in the first inoculation access, although PSV-S was transmitted to only one plant in the fourth and fifth inoculation access. These viruses may be transmitted in two phases by aphids, depending on the plant species. Received 16 April 1999/ Accepted in revised form 1 September 1999  相似文献   

Effects of highly unsaturated fatty acids on escape ability from moon jellyfish Aurelia aurita in red sea bream Pagrus major larvae     

SHINNOSUKE  NAKAYAMA  REIJI  MASUDA  TOSHIO  TAKEUCHI  MASARU  TANAKA 《Fisheries Science》2003,69(5):903-909

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In vitro progesterone production of corpus luteum in pregnant Mongolian gerbils (Meriones unguiculatus)     

Tadahiro ETOH  Masami TANAKA  Yutaka SONODA  Osamu KAI 《Animal Science Journal》2004,75(6):509-513

The present study was conducted to determine the in vitro progesterone (P₄) production by Mongolian gerbil (Meriones unguiculatus) corpus luteum (CL) on days 9, 18 and 22 of pregnancy in comparison with rat CL on days 9 and 16. The culture medium of one CL per well of a 96‐well plate was changed after 4 h incubation and the CL were further cultured for 24 h. The P₄ in serum and in the medium were measured by enzyme immunoassay system. Gerbil CL weight measured after 24 h incubation was at the same level for all 3 days of pregnancy and significantly different from that of the rat CL for the 2 days of pregnancy. The serum P₄ level in gerbils was high in early pregnancy and decreased in advanced days of pregnancy. In contrast, the P₄ in rats was higher in the latter half of pregnancy. In vitro P₄ production by a single CL after 4 h incubation decreased significantly during the days of pregnancy in both the gerbil and the rat. P₄ values produced by the gerbil CL for 24 h incubation were significantly higher than after 4 h incubation in all 3 days of pregnancy, however, there was no difference between the two incubation time groups in the rat. In vitro P₄ production by CL correlated well with the serum P₄ level on the 3 days of pregnancy in the gerbil. In the rat, however, the relationship was inversed between the two parameters. The correlation in this experiment suggested that CL is the sole source of P₄ for the maintenance of the gerbil pregnancy, with the result of abortion after an ovariectomy on day 20 of pregnancy as described in our previous report.  相似文献   

Polyoxin Resistance of Reddish Brown Laboratory Mutants of Cochliobolus heterostrophus     

Chihiro TANAKA  Kiminori SHIMIZU  Abdul GAFUR  Mitsuya TSUDA 《Journal of General Plant Pathology》2002,68(2):141-146

Six reddish brown polyxin-resistant mutants of Cochliobolus heterostrophus were isolated after ethyl methanesul-phonate and N-nitroquinoline oxide mutageneses followed by selection on polyoxin. All the mutants were highly resistant to polyoxin (MIC > 1600 μg/ml). When mutants were crossed with the wild-type strain, all crosses had a 1 : 1 ratio of mutant (reddish brown pigmentation and polyoxin resistance) : wild type (non-reddish brown pigmentation and polyoxin sensitivity), indicating that the phenotypes in these strains were due to alteration at a single gene locus in each strain. Allelism tests revealed the existence of two loci, Pol2 and Pol5. The results of the crossing and mutation-rate studies suggest that the each gene was pleiotropic for the reddish brown color and polyoxin resistance. Received 19 September 2001/ Accepted in revised form 25 December 2001  相似文献   

Effects of auxin and jasmonates on 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase gene expression during ripening of apple fruit     

Satoru Kondo  Siriwan Meemak  Yusuke Ban  Takaya Moriguchi  Takeo Harada 《Postharvest Biology and Technology》2009,51(2):281-284

1-Aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities, their gene expression, and ethylene production in apple fruit [Malus sylvestris (L.) Mill. Var. domestica (Borkh.) Mansf.] treated with a synthetic auxin 2,4-dichlorophenoxy-propionic acid (2,4-DP) and n-propyl dihydrojasmonate (PDJ), a jasmonic acid derivative, has been investigated to clarify the action of auxin and jasmonates on ethylene production. The fruit was harvested at 103 d after full bloom (preclimacteric). The expression of MdACS4 messenger RNA (mRNA) at 48 and 96 h after treatment was higher in fruit treated with 2,4-DP than in the untreated control, but those of MdACS1 and MdACO1 were not affected by treatment. The ethylene production in 2,4-DP-treated fruit increased at 96 h after treatment. In contrast, expression of mRNAs hybridized with MdACS1 and MdACO1 probes in the skin of PDJ-treated fruit were higher than those in the untreated control. In addition, ACC synthase activity and ethylene production also increased after treatment. These results show that the ethylene production rate may differ with the kind of genes which were stimulated by auxin or jasmonates.  相似文献   

Level of VERNALIZATION 1 expression is correlated with earliness in extra early-flowering mutant wheat lines     

Aiko Nishiura  Yusuke Kazama  Tomoko Abe  Nobuyuki Mizuno  Shuhei Nasuda  Koji Murai 《Breeding Science》2014,64(3):213-221

Four extra early-flowering mutants, named extra early-flowering1 (exe1), exe2, exe3, and exe4, were identified in Triticum monococcum strain KU104-1 following heavy-ion beam mutagenesis. The four exe mutants fell into two groups, namely Type I (moderately extra early-flowering type; exe1 and exe3) and Type II (extremely extra early-flowering type; exe2 and exe4). Analysis of plant development in a growth chamber showed that the speed of leaf emergence was accelerated in exe mutants at the reproductive stage compared to wild-type (WT) plants. The speed of leaf emergence was faster in Type II than Type I plants. Analysis of VERNALIZATION 1 (VRN1), a flowering promoter gene, showed that it was more highly expressed in seedlings at early developmental stages in Type II mutants than Type I mutants. These findings indicate that the difference in earliness between Type I and Type II mutants is associated with the level of VRN1 expression. The original KU104-1 is an einkorn wheat strain that carries a null allele of the VRN2 gene, a repressor of flowering. Thus, our results indicate that the level of VRN1 expression controls earliness in exe mutants independently of VRN2.  相似文献   

Quantitative mapping of kelp forests (Laminaria spp.) before and after harvest in coastal waters of the Shiretoko Peninsula,Hokkaido, Japan     

Kenji Minami  Naoki Tojo  Hiroki Yasuma  Yusuke Ito  Takahiro Nobetsu  Shin-ichi Fukui  Kazushi Miyashita 《Fisheries Science》2014,80(3):405-413

In the Shiretoko Peninsula, a World Natural Heritage site, the sustainable management of kelp forests has drawn public attention because of the economic and ecological importance of kelp. We spatially estimated the distributions of kelp forests in the Shiretoko Peninsula before and after harvest. Field surveys were conducted in coastal waters (23.74 km²) at the ends of July and August 2008, immediately before and after harvest. Data on the presence or absence and thickness of the kelp forests were collected via acoustic observation. The data were interpolated using geostatistical methods. Before harvest, the kelp forests were continuously distributed over 5.64 km² (thickness 33–132 cm), especially near the north part of the study area. After harvest, they were sparsely distributed over 2.73 km² (thickness 35–105 cm). In the southern part of the study area, the influence of harvests was observed as declines in forest area. In addition, relatively thickly forested areas formed the majority of the part most likely to be harvested. Selective harvesting for area and size was confirmed though quantitative mapping of kelp forests. The quantitative mapping of both the distribution and harvest of kelp forests was successful.  相似文献   

Resistance to <Emphasis Type="Italic">Phytophthora infestans</Emphasis>: exploring genes required for disease resistance in Solanaceae plants     

Daigo Takemoto  Yusuke Shibata  Makoto Ojika  Yuri Mizuno  Sayaka Imano  Mina Ohtsu  Ikuo Sato  Sotaro Chiba  Kazuhito Kawakita  Soriya Rin  Maurizio Camagna 《Journal of General Plant Pathology》2018,84(5):312-320

The oomycete Phytophthora infestans is the causal agent of potato late blight, one of the most destructive and historically significant pathogens in agricultural production. A virus-induced gene silencing-based screening of the solanaceous model plant N. benthamiana resulted in revealing a wide range of resistance mechanisms of solanaceous plants against this pathogen. In this article, we present an overview of the various pathways involved in the N. benthamiana–P. infestans pathosystem, including some of the follow-up work that was triggered by these findings. The purpose of this review is to assemble these findings and integrate them into our current understanding of plant pathogen defense mechanisms and discuss their potential application for the development of potato resistance to P. infestans.  相似文献