Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene     

Masashi WATANABE  Kazuaki TANAKA  Tatsuya TAKIZAWA  Kazuhito SEGAWA  Sakurako NEO  Ryo TSUCHIYA  Michiko MURATA  Masaru MURAKAMI  Masaharu HISASUE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):119-122

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Valproic Acid,a Histone Deacetylase Inhibitor,Decreases Proliferation of and Induces Specific Neurogenic Differentiation of Canine Adipose Tissue-Derived Stem Cells     

Yasuhiro KURIHARA  Takehito SUZUKI  Motoharu SAKAUE  Ohoshi MURAYAMA  Yoko MIYAZAKI  Atsushi ONUKI  Takuma AOKI  Miyoko SAITO  Yoko FUJII  Masaharu HISASUE  Kazuaki TANAKA  Tatsuya TAKIZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):15-23

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Effects of Cryopreservation on the Cell Viability,Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells     

Kazuya EDAMURA  Rei NAKANO  Kyohei FUJIMOTO  Kenji TESHIMA  Kazushi ASANO  Shigeo TANAKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):573-577

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Relationships Between the Appearances and Changes of Estrous Signs and the Estradiol-17β Peak,Luteinizing Hormone Surge and Ovulation During the Periovulatory Period in Lactating Dairy Cows Kept in Tie-stalls     

Toshiaki SUMIYOSHI  Tomomi TANAKA  Hideo KAMOMAE 《The Journal of reproduction and development》2014,60(2):106-114

Lactating Holstein-Friesian cows kept in tie-stall barn were used as subjects in this study. Rectal examination, ultrasonography and blood sampling were conducted every other day and then daily after the day on which diameter of the corpus luteum decreased. After the luteal diameter decreased for 2 consecutive days, rectal and ultrasound examinations, blood sampling, and observation of estrous signs were conducted at 6-h intervals. Most of the estrous signs became obvious with the increase in estradiol-17β (E₂) and became most remarkable 24 to 30 hours before ovulation, at which point the E₂ peak and luteinizing hormone (LH) surge were achieved, and then weakened which progression to ovulation. The correlation between the intensity of four estrous signs (hyperemia and swelling of the intravaginal part of the uterus, opening of the external uterine orifice and viscosity of the cervical mucus) and the plasma E₂ concentration was higher than that of three estrous signs (swelling of the vulva, contraction of the uterus, diameter of uterine horn) and the plasma E₂ concentration. The relaxation of the intravaginal part of the uterus showed a unique change compared with the other estrous signs, and it became most obvious 6, 12 and 18 h before ovulation; this obviously relaxed period was consistent with the generally accepted theoretical optimal time for artificial insemination (AI), i.e., 6 to 24 h after initiation of estrus. These results suggest that observation of estrous signs by vaginoscopic examination gave useful information for detection of the optimal timing of AI in the periovulatory period in lactating dairy cows kept in a tie-stall barn.  相似文献   

The Putative Promoters of Germ Cell-specific Genes and Nanog are Hypomethylated in Chicken Sperm     

Gakushi KITO  Hiroaki TANAKA  Tomoki SOH  Nobuhiko YAMAUCHI  Masa-aki HATTORI 《The Journal of reproduction and development》2014,60(3):224-229

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Physiological changes induced by salt intake in female Spontaneously Diabetic Torii‐Leprfa (SDT fatty) rat,a novel obese type 2 diabetic model     

Yoshiaki Katsuda  Yusuke Kemmochi  Mimi Maki  Ryuhei Sano  Yukihito Ishii  Katsuhiro Miyajima  Kochi Kakimoto  Takeshi Ohta 《Animal Science Journal》2014,85(5):588-594

Salt plays an important role in the control of blood pressure in obesity and diabetes mellitus. In this study, we investigated physiological changes such as blood pressure and renal function in salt‐loaded female Spontaneously Diabetic Torii‐Lepr^fa (SDT fatty) rats. SDT fatty rats were given 1% NaCl in drinking water for 14 weeks, from 4 to 18 weeks of age. Significant salt‐sensitive hypertension was observed in the salt‐loaded SDT fatty rats. Moreover, the salt‐loaded rats showed a decrease of creatinine clearance and deterioration on pathological renal findings, including glomerulosclerosis and tubular and interstitial lesions. Female SDT fatty rat is a useful model for investigating the mechanisms of high salt sensitivity in obesity and diabetes mellitus.  相似文献   

WIPPER: an accurate and efficient field phenotyping platform for large-scale applications     

Hiroe Utsushi  Akira Abe  Muluneh Tamiru  Yumiko Ogasawara  Tsutomu Obara  Emiko Sato  Yusuke Ochiai  Ryohei Terauchi  Hiroki Takagi 《Breeding Science》2015,65(3):285-289

More accurate, rapid, and easy phenotyping tools are required to match the recent advances in high-throughput genotyping for accelerating breeding and genetic analysis. The conventional data recording in field notebooks and then inputting data to computers for further analysis is inefficient, time-consuming, laborious, and prone to human error. Here, we report WIPPER (for Wireless Plant Phenotyper), a new phenotyping platform that combines field phenotyping and data recording with the aid of Bluetooth communication, thus saving time and labor not only for field data recoding but also for inputting data to computers. Additionally, it eliminates the risk of human error associated with phenotyping and inputting data. We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection. We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.  相似文献   

Dioxin Sensitivity-Related Two Critical Amino Acids of Arylhydrocarbon Receptor May Not Correlate with the Taxonomy or Phylogeny in Avian Species     

Nozomi FUJISAWA  Yusuke K. KAWAI  Shouta M. M. NAKAYAMA  Yoshinori IKENAKA  Hideaki YAMAMOTO  Mayumi ISHIZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1577-1583

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Background Data on Developmental Parameters During the Gestation Period in Rats     

Satoshi Furukawa  Seigo Hayashi  Masayoshi Abe  Souichiro Hagio  Kota Irie  Yusuke Kuroda  Izumi Ogawa  Akihiko Sugiyama 《Journal of toxicologic pathology》2013,26(1):83-88

Abstract: Background data during the gestation period were obtained from 128 Wistar Hannover GALAS rats and 26 Crl:CD(SD) pregnant rats in the control groups of our previous toxicity studies. The body weights of dams in the Wistar Hannover GALAS rats were significantly lower throughout the gestation period than those in the Crl:CD(SD) rats. In contrast, the time-dependent change in the body weight gain (%) of dams showed very similar trends in both strains. The mean number of live embryos/fetuses in the Wistar Hannover GALAS rats was 12.0, and was lower than that (14.5) in the Crl:CD(SD) rats. The placental weights gradually increased with pregnancy progression and reached a plateau on gestation day (GD) 19, although the embryo/fetal weights rapidly increased from GD 17 to GD 21. The embryo/fetal weights in the Wistar Hannover GALAS rats were significantly lower on only GD 21 than those in the Crl:CD(SD) rats. It is considered that this fetal weight difference between the strains develops during the fetal period, but not during the organogenesis period. In contrast, there were no differences in the placental weights between the two strains. Microscopically, the thickness of the labyrinth zone in the Wistar Hannover GALAS rats was thicker throughout the gestation period than that in the Crl:CD(SD) rats.  相似文献   

A 4-week Repeated Dose Toxicity Study of Glycine in Rats by Gavage Administration     

Yusuke Shibui  Tadashi Miwa  Mayumi Yamashita  Keigi Chin  Terutaka Kodama 《Journal of toxicologic pathology》2013,26(4):405-412

In order to examine the toxicity profile of glycine, an authorized food additive, a solution of glycine in water for injection was administered orally (via gavage) to male SD rats (Crl:CD(SD)) once daily for 4 weeks at doses of 500, 1000 and 2000 mg/kg/day in a volume of 10 mL/kg. Control animals received vehicle only. No animals died, and no glycine-related changes were observed in body weight, food consumption, water consumption, hematology, organ weight, gross pathological examination or histopathological examination. In urinalysis, daily urinary volume and urinary Cl excretion were significantly higher in the 2000 mg/kg/day dose group, and urine pH and urinary protein showed lower trends in the glycine-treated groups. However, these changes were considered to be of little toxicological significance, because there were no histopathological changes in the kidneys or urinary bladder and no changes in other urinary parameters. As regards blood chemistry, phospholipids were significantly higher in the 2000 mg/kg/day dose group. However, the increase was small and was not considered to be toxicologically significant. In conclusion, none of the animals in any of the glycine-treated groups showed changes that were considered toxicologically significant. Therefore, the no-observed-adverse-effect level of glycine was estimated to be at least 2000 mg/kg/day under the conditions of this study.  相似文献