Retrotransposon-like elements in the genome of Verticillium dahliae may be used as DNA markers for fungal species and pathotypes     

Toshiyuki Usami  Masahiro Shishido  Yoshiyuki Ebihara  Yuta Kamigahira  Yoshimiki Amemiya 《Journal of General Plant Pathology》2005,71(2):117-123

Using differential hybridization, two DNA fragments, VDf35 and VDf90, specific to Verticillium dahliae, were isolated. These fragments contained truncated open reading frames (ORFs) homologous to the gypsy-type retrotransposon. The ORFs of VDf35 and VDf90 were pol and gag homologs, respectively. In addition, VDf90 had a pol homolog without an ORF sequence. The pol homologs in VDf35 and VDf90 were similar to each other, and these two DNA fragments had completely identical sequences. Genomic Southern analysis revealed that numerous copies of these homologs existed in V. dahliae, suggesting that V. dahliae carries a gypsy-like retroelement. Genomic Southern and polymerase chain reaction (PCR) analysis also indicated that a large number of these homologs exist in V. longisporum as well as in V. dahliae, but only a few were present in V. albo-atrum. No homolog was found in either V. nigrescens or V. tricorpus. The uneven distribution of these homologs of the retroposon-like elements among Verticillium species suggested a close genetic kinship between V. dahliae and V. longisporum. PCR primers designed from VDf35 showed species- or pathotype-specific amplification. Therefore, this sequence may be useful as a DNA marker to identify species and pathotypes of V. dahliae. This is the first report on a retrotransposon-like sequence in the genome of phytopathogenic Verticillium species.The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AB095264 (VDf90) and AB095265 (VDf35)  相似文献   

Monoaminergic Innervation of the Caudal Neurosecretory System of the Carp, Cyprinus carpio     

Yuta  Kovatashi  Hideshi  Kobatashi  Susumu  Ohshiro  Yoshitsugu  Osmui Motohstsu  Fujiwar 《Anatomia, histologia, embryologia》1980,9(1):65-72

Monoamine fluorescence was studied in the caudal neurosecretory system of eighty-four adult carp, Cyprinus carpio, of either sex. Some neurosecretory cells were surrounded with monoaminergic fluorescent varicosities, while others were not. The varicosities werc: also found adjacent to nerve terminals or capillaries in the urophysis. Noradrenaline, but not adrenaline or dopamine, was detected memically in the urophysis and the spinal cord. These results suggest that noradrenergic neurons seem to regulate both the synthesis and the release of neurosecretory material in the carp caudal neurosecretory system.  相似文献   

Pathways of glucose metabolism in corneal epithelium     

KINOSHITA JH  MASURAT T  HELFANT M 《Science (New York, N.Y.)》1955,122(3158):72-73

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Estrogen increases KISS1 expression in newly generated immortalized KISS1-expressing cell line derived from goat preoptic area     

Yukina OSHIMO  Arisa MUNETOMO  Fumie MAGATA  Yuta SUETOMI  Shuhei SONODA  Yukari TAKEUCHI  Hiroko TSUKAMURA  Satoshi OHKURA  Fuko MATSUDA 《The Journal of reproduction and development》2021,67(1):15

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Uterine Carcinosarcoma in a 2-year-old Female Wistar Hannover GALAS Rat     

Takami S  Ogawa K  Umemura T  Hibi D  Ishii Y  Okamura T  Tasaki M  Inoue T  Suzuki Y  Jin M  Cho YM  Nishikawa A 《Journal of toxicologic pathology》2011,24(1):63-67

Carcinosarcomas are rare tumors in humans as well as rats and most commonly occur in the uterus. Recently, we observed a case of incidental carcinosarcoma of the uterus in a female Wistar Hannover GALAS [BrlHan:WIST@ Jcl (GALAS)] rat at 2 years of age. Histopathologically, the tumor was characterized by an admixture of malignant epithelial and nonepithelial elements. The carcinomatous components represented a type of endometrial carcinoma, consisting of glandular and solid proliferation of large-sized tumor cells. Prominent mitoses and tumor cell invasion were observed. The sarcomatous components were characterized by multifocal proliferation of severe atypical cells with cartilage matrix and were diagnosed as chondrosarcoma. Transitions between carcinomatous and sarcomatous components were observed, and many tumor cells in the solid lesion showed immunohistochemical reactivity with both cytokeratin and vimentin. Based on these findings, this tumor was diagnosed as a uterine carcinosarcoma. This is the first report of uterine carcinosarcoma in Wistar Hannover GALAS [BrlHan:WIST@Jcl (GALAS)] rats.  相似文献   

Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica)     

Mami SERIZAWA  Mihoko KINOSHITA  Daniela RODLER  Akira TSUKADA  Hiroko ONO  Takashi YOSHIMURA  Norio KANSAKU  Tomohiro SASANAMI 《Animal Science Journal》2011,82(2):227-235

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The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes     

Inoue T  Nonaka N  Kanai Y  Iwaki T  Kamiya M  Oku Y 《Veterinary parasitology》2007,150(1-2):88-96

Anthelmintic (praziquantel) baiting of wild red foxes against Echinococcus multilocularis infection was studied in a highly epizootic suburban area of Otaru, Hokkaido (the northern island of Japan) during the summer and autumn in the years 1999-2004. Acceptance of baits containing the biomarker tetracycline (TC) was evaluated. The prevalence of E. multilocularis infection in foxes before baiting (1999-2000) was 58% (88/153), whereas in the fourth year of bait distribution year (2004), it decreased to 11% (5/45). Analysis of TC marking in the teeth of foxes showed that 39% (77/195) of those captured after baiting were estimated to have consumed baits in the year of capture. Importantly, more juvenile (56%, 49/87) than adult foxes (26%, 28/108) were marked, indicating efficient baiting of juveniles, which tended to have a higher worm burden of E. multilocularis. Of 77 marked foxes, E. multilocularis and Alaria alata (monitored as the second indicator species of deworming) were not detected in 70 (90%) and 76 (99%) foxes, respectively. The results suggest effective deworming by bait consumption. However, it was also demonstrated that 9% of the marked foxes were infected or re-infected after bait consumption, suggesting high infection pressure and the importance of frequent baiting.  相似文献   

Development of loop-mediated isothermal amplification methods for detecting Taylorella equigenitalis and Taylorella asinigenitalis     

Yuta KINOSHITA  Hidekazu NIWA  Yoshinari KATAYAMA  Kazuhisa HARIU 《Journal of Equine Science》2015,26(1):25-29

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Resistance to anthracnose is decreased by tissue culture but increased with longer acclimation in the resistant strawberry cultivar     

Kiyoshi Namai  Yuta Matsushima  Masaji Morishima  Masayuki Amagai  Tomohide Natsuaki 《Journal of General Plant Pathology》2013,79(6):402-411

Inoculation of tissue-cultured plants of strawberry cultivar Ichigochukanbohon-Nou2gou (Nou-2) with the anthracnose pathogen (Glomerella cingulata) results in wilting and plant death, whereas inoculation of strawberry runners grown in greenhouses results in leaf spots, not wilting or death. When tissue-cultured Nou-2 plants were acclimated for 3, 9 or 15 days, plant resistance to anthracnose increased as the acclimation period increased, suggesting that the resistance of Nou-2 may be induced by external factors. To clarify the mechanisms of resistance, we used cDNA microarray analyses to compare gene expressions among tissue-cultured and acclimated plants of the resistant cultivar Nou-2 and the susceptible cultivar Tochiotome. As a result, we identified 18 cDNA clones that were upregulated during acclimation in Nou-2 but not in Tochiotome. In a real-time RT-PCR of the 18 clones, the expression levels of three were significantly higher in acclimated plants of Nou-2. Two of the three clones showed close homology to enzymes related to flavonoid biosynthesis, i.e., leucoanthocyanidin dioxygenase (LDOX, ANS) and UDP-glucosyltransferase, putative (3-GT). The clones spotted onto the cDNA microarrays were rechecked, and 23 nonredundant cDNA clones of 13 enzymes were estimated to be flavonoid biosynthetic enzymes. Most of these clones were upregulated by acclimation in Nou-2.  相似文献   

Transactivational property of chemicals via medaka glucocorticoid receptor 1b using a stable reporter gene assay     

Kazuya Sato  Masaki Okamoto  Yuta Suzuki  Kagehisa Kiba  Hiroki Tatsumi  Hisashi Teramura  Toshitaka Ikeuchi 《Fisheries Science》2013,79(6):943-948

The transactivational property of natural and synthetic chemicals via medaka GR1b was investigated after development of a stable cell line for the reporter gene assay. In our study, cortisol was the most potent agonist among the natural corticoids assayed for potency [EC₅₀ (concentration of agonist provoking a response halfway between the baseline and maximum response) 68 nM] and efficacy. Three artificial corticosteroids, namely, dexamethasone [EC₅₀ 16 nM, relative agonistic activity to cortisol (RAA) 144 %], prednisolone (EC₅₀ 81 nM, RAA 116 %) and clobetasol propionate (EC₅₀ 0.10 nM, RAA 220 %), showed strong agonistic activity and were more potent than the original corticoid, F. All synthetic corticoids used in our study were full agonists. Interestingly, melengestrol acetate, a synthetic progestogen, induced luciferase activity in a dose-dependent manner. Based on its EC₅₀ value and RAA of 29 nM and 57 %, respectively, this molecule was assessed as a partial agonist. None of the other steroids and chemicals assayed in our study induced an agonistic response. In conclusion, we successfully developed a stable reporter gene assay that can be used to assess the transactivational property of glucocorticoid-like chemicals toward medaka GR1b.  相似文献