Impact of aluminium stress on oxalate and other metabolites in Rumex obtusifolius     

A MIYAGI  M UCHIMIYA  M KAWAI‐YAMADA  H UCHIMIYA 《Weed Research》2013,53(1):30-41

To understand alterations of oxalate and other metabolite levels induced by aluminium ion (Al³⁺) stress in Rumex plants, we measured the metabolites in R. obtusifolius using the capillary electrophoresis–mass spectrometry. Oxalate and its precursors (isocitrate and citrate) accumulated in leaves of R. obtusifolius after the Al³⁺ treatment at pH 4.5. Such increase was not observed under the acidic condition (pH 4.5) without Al³⁺. Principal component analysis showed organ‐specific changes in metabolite levels in R. obtusifolius by the Al³⁺ treatment. Highly positive correlations between oxalate and its precursors were revealed by hierarchical clustering and correlation analyses. An increase in oxalate content was consistently observed for three Rumex species (R. obtusifolius, R. crispus and R. japonicus) grown in the presence of Al³⁺. On the other hand, multivariate analyses revealed the differential alterations of other metabolite levels between R. obtusifolius and the other two Rumex species.  相似文献   

Valproic Acid Treatment from the 4-cell Stage Improves Oct4 Expression and Nuclear Distribution of Histone H3K27me3 in Mouse Cloned Blastocysts     

Yuuki ISAJI  Moeko MURATA  Naoya TAKAGUCHI  Toshita MUKAI  Yosuke TAJIMA  Hiroshi IMAI  Masayasu YAMADA 《The Journal of reproduction and development》2013,59(2):196-204

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The proteins synthesized in tissue infected with tobacco mosaic virus     

COMMONER B  YAMADA M  RODENBERG SD  WANG TY  BASLER E 《Science (New York, N.Y.)》1953,118(3071):529-534

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Dr. Yohei Sato's donation to PAWEES     

Yutaka Matsuno 《Paddy and Water Environment》2005,3(4):251-251

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PGF<Subscript>2α</Subscript> and gonadal steroid plasma levels of successful and unsuccessful spawning <Emphasis Type="Italic">Piaractus mesopotamicus</Emphasis> (Teleostei,Characiformes) females     

Rafael Yutaka Kuradomi  Sergio Ricardo Batlouni 《Aquaculture International》2018,26(4):1083-1094

Gonadal steroid and prostaglandin F₂α (PGF_2α) plasma levels were evaluated in successfully (SP) and unsuccessfully ovulated (UN) female Piaractus mesopotamicus. Forty-one females were injected with crude carp pituitary extract (0.6 and 5.4 mg kg^?1 with a 24-h interval between the doses) and sampled to determine the plasma concentration of 17β-estradiol (E₂), 17α-hydroxyprogesterone (17α-OHP), 17α,20β-dihydroxy-4-pregnen-3-one (DHP), PGF_2α, and testosterone (T) after each injection (first—A1 and second—A2), and at the time of ovulation for SP and UN. Two clusters were obtained using multivariate analysis: 1—composed of all A1, all A2, and some UN; and 2—composed of all SP and some UN. Median values of E₂ plasma levels were similar between clusters; however, plasma levels of T, 17α-OHP, DHP, and PGF_2α of cluster 2 (predominantly formed by SP) were higher than those of cluster 1. Since cluster 2 contained all SP and females of this cluster presented higher levels of PGF_2α, T, 17α-OHP, and DHP, here we evidently shown in an unprecedented manner that concomitant increased levels of these substances were associated with successful ovulation in this species, but such an increase was not determinant for successful ovulation due to the presence of some UN females in the same cluster 2. These findings highlight the unexplored potential of PGF_2α to be used as an accessory tool for inducing successful ovulation for fish farming purposes.  相似文献   

Spawning induction of blue mackerel <Emphasis Type="Italic">Scomber australasicus</Emphasis> and eastern little tuna <Emphasis Type="Italic">Euthynnus affinis</Emphasis> by oral administration of a crude gonadotropin-releasing hormone analogue     

Kotaro Amezawa  Ryosuke Yazawa  Yutaka Takeuchi  Goro Yoshizaki 《Fisheries Science》2018,84(3):495-504

Scombridae species, such as tunas and mackerels, often do not spawn in land-based fish tanks without hormone treatment. To induce spawning in various fishes, a gonadotropin-releasing hormone analogue (GnRHa) is often administered by pellet implantation. Noninvasive administration is desired to induce spawning in scombrids that are sensitive to handling stress. Spawning induction by oral administration has been reported in several fishes, yet this method has not been put into practice in the aquaculture industry since a considerable amount of GnRHa is needed. Utilization of peptide synthesizers is widespread, and antigen-grade GnRHa (AgGnRHa) produced by a custom-peptide supplier is approximately 100-fold cheaper than conventional reagent-grade GnRHa (RgGnRHa), although the purity of AgGnRHa is lower. Here, we confirmed that the spawning induction potency of AgGnRHa was similar to that of RgGnRHa by pellet implantation in blue mackerel Scomber australasicus. Oral administration of AgGnRHa [6.0 mg/kg body weight (BW) per day] showed an equivalent ability to induce spawning of the mackerel as pellet implantation (0.1 mg/kg BW). We could also induce spawning of eastern little tuna Euthynnus affinis by oral administration of the AgGnRHa. Further, the obtained eggs showed higher survival. Thus, the oral delivery of AgGnRHa could be a powerful tool to induce spawning in Scombridae.  相似文献   

Complete mitochondrial DNA sequence of the Japanese flying fish <Emphasis Type="Italic">Cypselurus hiraii</Emphasis>   总被引：2，自引：0，他引：2  

Mitsutoshi?NagaseEmail author  Tadanori?Aimi  Katsuaki?Suginaka  Yutaka?Kitamoto  Tsutomu?Morinaga 《Fisheries Science》2005,71(4):914-923

ABSTRACT:   In this study, to develop a technique that enables authentication of processed seafood, the complete nucleotide sequence of the mitochondrial genome for the Japanese flying fish Cypselurus hiraii was determined. Three segments spanning the entire genome were amplified using polymerase chain reaction, and products were subsequently used as templates for direct sequencing with 60 primers. The genome (16 528 base pairs) was found to contain the same 37 genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrate mitochondrial genomes, with the gene order being identical to that typical of vertebrates. A major non-coding region between the tRNA^Pro and tRNA^Phe genes (868 base pairs) appears to be the control (D-loop) region, as it has several conserved blocks characteristic of control regions.  相似文献   

Improvement of ovulation induction by additive injection of 17,20β‐Dihydroxy‐4‐pregnen‐3‐one after human chorionic gonadotropin administration in a pelagic egg spawning marine teleost,nibe croaker Nibea mitsukurii (Jordan & Snyder)          下载免费PDF全文

Yoji Yamamoto  Takashi Yatabe  Kentaro Higuchi  Yutaka Takeuchi  Goro Yoshizaki 《Aquaculture Research》2015,46(6):1323-1331

This study aimed to develop the consistent ovulation induction method in a pelagic egg spawning marine teleost, nibe croaker Nibea mitsukurii. Attempts to induce oocyte maturation and ovulation in nibe croaker using human chorionic gonadotropin (hCG; 0.5 IU g^?1) resulted in the normal progression of oocyte maturation and hydration, but a failure to induce ovulation in many individuals. This ovulation disorder was similarly observed even when the dose of hCG was increased 10 times (5 IU g^?1) or decreased to one tenth (0.05 IU g^?1), indicating that it cannot be completely overcome solely by hCG administration. However, this ovulation disorder could be completely overcome by subsequent administration of 17,20β‐dihydroxy‐4‐pregnen‐3‐one (DHP) at the appropriate dose (0.5 μg g^?1) and time (20 h after hCG administration). An increase in the number of individuals that ovulated due to DHP administration led to an increase in individuals producing larvae, resulting in an approximately threefold increase in the estimated number of larvae produced compared with the group of fish administered hCG alone. Thus, this ovulation induction method using DHP administration after hCG was demonstrated to overcome the ovulation disorder in nibe croaker and could be applicable to commercially important species with similar ovulation problems.  相似文献