Recovery of Mycoplasma spp. from the Reproductive Tract of the Mare during the Estrous Cycle          下载免费PDF全文

Bermudez V  Miller R  Johnson W  Rosendal S  Ruhnke L 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):519-522

The sites in the genital tract from which mycoplasmas could be recovered at various stages of the estrous cycle were studied in five Standardbred mares naturally infected with Mycoplasma. Mycoplasma equigenitalium and Mycoplasma subdolum were most frequently isolated from the clitoral fossa as compared to the vagina, cervix, and uterus. The lowest isolation prevalence was observed in the uterus. The recovery of Mycoplasma spp. from the clitoral fossa did not differ at any stage of the estrous cycle; however, recovery from the vagina, cervix, and uterus was variable during the cycle and more organisms were recovered on the day of ovulation than at any other time. From these results it was concluded that the clitoral fossa is the most likely “ecological niche” for Mycoplasma spp. in the mare. Ureaplasmas were not isolated.  相似文献   

Detection of an atypical (possibly Group C) rotavirus in New Zealand pigs     

Fu ZF 《New Zealand veterinary journal》1987,35(7):115-116

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Haemagglutination-inhibiting antibodies against arboviruses of the families Togaviridae and Bunyaviridae in birds caught in southern Moravia, Czechoslovakia     

Z Juricová  Z Hubálek  J Halouzka  J Pellantová  J Chytil 《Folia parasitologica》1987,34(3):281-284

A total of 295 birds belonging to 19 species of 7 families of wild Passeriformes were examined by haemagglutination-inhibition test. The birds were caught for an international research program "Balt" at the time of autumn migration (August-September 1984). Their blood sera were examined for antibodies against 6 arbovirus antigens of the genera Alphavirus (Sindbis-SIN) and Flavivirus (tick-borne encephalitis-TBE, West Nile-WN) and family Bunyaviridae (Tahyna-TAH, Calovo-CVO and Bhanja-BHA). Antibodies against all studied viruses were detected at different frequencies: SIN 6.4%, TBE 7.1%, WN 9.7%, TAH 16.3%, CVO 12.1%, and BHA 1.0%.  相似文献   

Transmutation and functional representation of heterogeneous landscapes   总被引：3，自引：0，他引：3  

Anthony W. King  Alan R. Johnson  Robert V. O'Neill 《Landscape Ecology》1991,5(4):239-253

Models of local small-scale ecological processes can be used to describe related processes at larger spatial scales if the influences of increased scale and heterogeneity are carefully considered. In this paper we consider the changes in the functional representation of an ecological process that can occur as one moves from a local small-scale model to a model of the aggregate expression of that process for a larger spatial extent. We call these changes spatial transmutation. We specifically examine landscape heterogeneity as a cause of transmutation. Spatial transmutation as a consequence of landscape heterogeneity is a source of error in the prediction of aggregate landscape behavior from smaller scale models. However, we also demonstrate a procedure for taking advantage of spatial transmutation to develop appropriately scaled landscape functions. First a mathematical function describing the process of interest as a local function of local variables is defined. The spatial heterogeneity of the local variables is described by their statistical distribution in the landscape. The aggregate landscape expression of the local process is then predicted by calculating the expected value of the local function, explicitly integrating landscape heterogeneity. Monte Carlo simulation is used to repeat the local-to-landscape extrapolation for a variety of landscape patterns. Finally, the extrapolated landscape results are regressed on landscape variables to define response functions that explain a useful fraction of the total variation in landscape behavior. The response functions are hypotheses about the functional representation of the local process at the larger spatial scale.  相似文献   

Canine von Willebrand's disease. A heterogeneous group of bleeding disorders     

G S Johnson  M A Turrentine  K H Kraus 《Veterinary Clinics of North America: Small Animal Practice》1988,18(1):195-229

The term "von Willebrand's disease," refers to a group of inherited bleeding disorders, all of which are caused by a deficiency of the multimeric plasma glycoprotein, von Willebrand factor. The various forms of canine von Willebrand's disease can be categorized into one of three major types: in type I canine von Willebrand's disease, all sizes of von Willebrand factor multimers can be detected in the plasma; in type II canine von Willebrand's disease, only the smaller von Willebrand factor multimers are found in the plasma (larger multimers are absent); and in type III canine von Willebrand's disease, von Willebrand factor is completely absent from the plasma or present in only trace amounts. Von Willebrand's disease is common in dogs, but some forms of the disease are so mild that they are of questionable clinical significance.  相似文献   

Estimating dispersibility of Acer,Fraxinus and Tilia in fragmented landscapes from patterns of seedling establishment     

W. Carter Johnson 《Landscape Ecology》1988,1(3):175-187

Relative dispersibility of Tilia americana L., Acer saccharum Marsh. and Fraxinus pennsylvanica Marsh, was inferred from the ratio among species-specific regression coefficients () computed from seedling density-distance plots. Density counts were made in spatially-uniform old fields adjacent to single seed sources or monotypic fencerows. Resultant seedling shadow curves approximate the negative exponential form expected for many seeds (log y=a–X). This basic curve shape fit species of differing dispersibility, dispersal under a range of wind directions and one-year-old or all-aged cohorts. The ratios of were 1:2.6:3.2 for Tilia, Acer and Fraxinus, respectively, in order of increasing dispersibility. Vegetation patches isolated from seed sources by several hundred meters or more should have extremely low input of seeds, especially Tilia and Acer.The finding that Fraxinus disperses farther than Acer was unexpected, since the samaras of the former have faster terminal velocities. The relationship can be explained by better performance of Fraxinus samaras in the stronger winds experienced by trees in open landscapes, poorer formation of the samara abscission layer, and release of samaras following leaf abscission and during the winter when winds are the strongest. Both the samara plan and dispersal phenology need to be considered in estimating relative dispersibility among species.  相似文献   

Porcine parvovirus infection in a commercial piggery     

Hampson DJ  Moller K  Robertson ID  Fu ZF 《New Zealand veterinary journal》1986,34(5):74

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Some diagnostic, biologic and morphologic characteristics of Francisella tularensis strains isolated from the ticks Ixodes ricinus (L.) in the Prague agglomeration   总被引：2，自引：0，他引：2  

D Málková  K Blazek  V Danielová  J Holubová  M Lavicková  Z Marhoul  J Schramlová 《Folia parasitologica》1986,33(1):87-95

Four infectious agents were isolated from the ticks Ixodes ricinus (L.) collected in the recreational area and park-forest of Prague. On the basis of cultivation, staining, biochemical, serologic properties, pathogenicity for animals and histological tests they were identified as Francisella tularensis with the following features: they are short, gram-negative rods of approximate dimensions of 0.3 X 0.8 micron, growing in enriched media after 3-4 day incubation at 37 degrees C. They form small circular, at first transparent, later greyish turbid colonies with regular rims. They are little active biochemically. They are susceptible to streptomycin and some broad spectrum antibiotics. They react positively with tularemic serum, but in lower titres than those in which this serum reacts with standard antigen. The microbes are highly pathogenic for mice, guinea-pigs, young rats, in which a massive bacteriemia occurs before death, but they do not kill rabbits. They multiply well in chick embryo, but do not grow in cell or tissue cultures. The most important histologic changes were observed in liver and spleen of mice. No pathologic changes were found in brain, lungs, heart, kidneys. Necroses were found in liver and in their marginal zones the microbes were present. Conspicuous were changes in numerous hepatocytes which became enlarged due to microbial multiplication and finally transformed into "sacs" packed with microbes. Histological and electronoptical examination showed that these are intracellular parasites fringed with a light lytic zone. Discussed is the problem to what extent the properties of the isolated strains are typical of F. tularensis as well as the importance of their detection from the aspect of epidemiology and differential diagnostics.  相似文献   

Cytochemical properties of chicken blood cells resembling both thrombocytes and lymphocytes     

Swayne DE  Stockham SL  Johnson GS 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1986,15(2):17-24

Generally accepted criteria were used to identify typical nucleated thrombocytes and typical small lymphocytes in chicken-blood smears subjected to modified-Wright staining. Other cells, here referred to as "intermediate cells," were difficult to classify because in some aspects they resembled thrombocytes while they also had features typical of small lymphocytes. The "intermediate cells" had small, round or oval nuclei with coarsely condensed chromatin, characteristic of both thrombocytes and small lymphocytes. In addition, "intermediate cells" had moderately abundant cytoplasmic volumes, typical of thrombocytes but blue cytoplasm lacking both granules and vacuoles, which is characteristic of small lymphocytes. It made little difference to the thrombocyte count whether these cells were classified as thrombocytes or small lymphocytes; however, this decision made a substantial difference to the lymphocyte count in some chicken-blood smears. Most "intermediate cells" (351 of 410 cells examined) were nonfluorescent after treatment with formaldehyde gas. Furthermore, most "intermediate cells" failed to acquire characteristic pigments when subjected to either Grimelius staining (179 of 204 cells examined) or periodic acid-Schiff staining (173 of 206 cells examined). Typical small lymphocytes reacted in the same way, failing to fluoresce after gaseous formaldehyde treatment (65 of 65 cells examined) and failing to react during Grimelius staining (41 of 44 cells examined) or periodic acid-Schiff staining (21 of 21 cells examined). In contrast, almost all typical thrombocytes became fluorescent in response to gaseous formaldehyde (709 of 718 cells examined) and gave positive reactions when subjected to Grimelius staining (381 of 382 cells examined) or periodic acid-Schiff staining (322 of 326 cells examined). These findings suggested that "intermediate cells" should be classified as lymphocytes in differential cell counts.  相似文献   

Isolation of avian influenza virus (subtype H5N2) from chicken eggs during a natural outbreak     

D T Cappucci  D C Johnson  M Brugh  T M Smith  C F Jackson  J E Pearson  D A Senne 《Avian diseases》1985,29(4):1195-1200

Avian influenza virus (A/Chicken/Pennsylvania/83; H5N2) was recovered from the yolk, albumen, and shell surface of eggs obtained from naturally infected chicken flocks in Pennsylvania and Virginia. These findings represent the first reported isolation of avian influenza virus from the internal contents of eggs from naturally infected flocks. The need for adequate safeguards to prevent spread of the virus during commercial movement of table and hatching eggs, cracked and "checked" eggs, and egg flats and other materials is emphasized.  相似文献