The contribution of root respiration of<Emphasis Type="Italic">Pinus koraiensis</Emphasis> seedlings to total soil respiration under elevated CO<Subscript>2</Subscript> concentrations   总被引：5，自引：0，他引：5  

Ying?Liu  Shi-jie?HanEmail author  Xue-feng?Li  Yu-mei?Zhou  Jun-hui?Zhang  Xia?Jia 《林业研究》2004,15(3):187-191

The impacts of elevated atmospheric CO₂ concentrations (500 μmol·mol⁻¹ and 700 μmol·mol⁻¹) on total soil respiration and the contribution of root respiration ofPinus koraiensis seedlings were investigated from May to October in 2003 at the Research Station of Changbai Mountain Forest Ecosystems, Chinese Academy of Sciences, Jilin Province, China. After four growing seasons in top-open chambers exposed to elevated CO₂, the total soil respiration and roots respiration ofPinus koraiensis seedlings were measured by a Li-6400-09 soil CO₂ flux chamber. Three PVC cylinders in each chamber were inserted about 30 cm into the soil instantaneously to terminate the supply of current photosynthates from the tree canopy to roots for separating the root respiration from total soil respiration. Soil respirations both inside and outside of the cylinders were measured on June 16, August 20 and October 8, respectively. The results indicated that: there was a marked diurnal change in air temperature and soil temperature at depth of 5 cm on June 16, the maximum of soil temperature at depth of 5 cm lagged behind that of air temperature, no differences in temperature between treatments were found (P>0.05). The total soil respiration and soil respiration with roots severed showed strong diurnal and seasonal patterns. There was marked difference in total soil respiration and soil respiration with roots severed between treatments (P<0.01); Mean total soil respiration and contribution of root under different treatments were 3.26, 4.78 and 1.47 μmol·m⁻²·s⁻¹, 11.5%, 43.1% and 27.9% on June 16, August 20 and October 8, respectively. Foundation item: This study was supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (KZCX1-SW-01) and the National Natural Science Foundation of China (30070158). Biography: LIU Ying (1976-), female, Ph. D. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China. Responsible editor: Song Funan  相似文献   

Characterisation of and changes in the atmospheric deposition situation in German forest ecosystems using multivariate statistics     

Nicole?WellbrockEmail author  Winfried?Riek  Barbara?Wolff 《European Journal of Forest Research》2005,124(4):261-271

Based on the results of the atmospheric deposition classification of the year 1989, a methodical approach should be introduced, which—based on the modelled total deposition rates—enables us to characterise the input situation of forest monitoring plots and to delimit load areas in Germany. In 1989, the deposition situation in nearly 1,800 forest monitoring sites (BZE/extensive Soil Condition Inventory) in Germany could be explained by four factors (or three, excluding sea salt impact) with the help of a factor analysis. The factor values were grouped into six deposition types with typical compounds and regional patterns. The classified input rates of the soil inventory plots adequately represent the stress situation and deposition changes in Germany. The application of the statistical approach on the level of Brandenburg clarifies the special local input situation. Due to the special combination of deposed elements, the sources of emissions can be characterised as well. When the soil inventory is repeated, a project planned for 2006, this approach can be used in order to determine homogenous areas for stratified data evaluation.  相似文献   

长白山阔叶红松林CO2廓线和CO2贮存的动态     

韩士杰  林丽莎  于贵瑞  张军辉  吴家兵  赵晓松  关德新 《林业研究》2003,14(4):275-279

从1999年8至10月,2000年的4至6月,2002年8月至2003年9月,在平均树高为26米的长白山阔叶红松林内,用红外气体分析仪(2250D,LI-CORInc.和LI-COR,820)测定了不同高度的二氧化碳浓度。根据测定的数据,分析了阔叶红松林二氧化碳廓线的日变化和季节变化动态。结果表明:CO2浓度的垂直分布在白天和夜间是不同的,在接近地面处CO2浓度始终最大。从季节CO2廓线看出,在植物生长季节林冠处CO2浓度有明显的成层现象,不同高度(60~2.5m)的CO2浓度3月份变化较小差值为10mmol穖ol-1,而在7月份变化较大,差值为60mmol穖ol-1。7月份林冠处(22,26,32m)CO2浓度梯度较大,浓度差为8mmol穖ol-1。计算位于涡度相关仪器之下的40米高空气柱中CO2贮存状况表明,年际贮存是负值,但对NEE的贡献很小。图4参11。  相似文献   

葡萄糖酸亚铁固体脂质纳米粒的制备工艺研究     

王琳琳  童群义 《安徽农业科学》2016,44(16):90-93

[目的]探索葡萄糖酸亚铁固体脂质纳米粒的制备方法。[方法]采用两步W/O/W乳化方式制备葡萄糖酸亚铁固体脂质纳米粒(SLN)。以包埋率为指标,研究内外相、油水相体积比,卵磷脂浓度对SLN包埋率的影响。[结果]试验确定当内相中水相与油相的体积比为0.2,外水相与内相乳状液的体积比为5∶1,卵磷脂浓度为5%时,SLN的包埋率取得最大值,为52.48%。在内水相引入海藻酸钠(1∶2,W/W)的结果表明,SLN的包埋率增大,为59.03%,粒径、Zeta电位和多分散系数发生变化。体外释放试验表明,SLN能起到保护葡萄糖酸亚铁经受胃酸环境的作用,并在模拟肠液中表现为突释的释放效果。[结论]固体脂质纳米粒对葡萄糖酸亚铁有较好的保护及释放效果,可为无机铁补充剂的生产提供新的技术手段。  相似文献   

Interaction of sub-epithelial connective tissue components with Staphylococcus aureus and coagulase-negative staphylococci from bovine mastitis   总被引：4，自引：0，他引：4  

W Mamo  G Fr?man  T Wadstr?m 《Veterinary microbiology》1988,18(2):163-176

Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from bovine mastitis were examined for their ability to interact with 125I-labelled fibronectin, fibrinogen and type II collagen. Their relative surface hydrophobicity and production of extracellular capsule were also investigated. Almost all S. aureus strains bound fibronectin (mean value 23%), fibrinogen (mean value 12%) and type II collagen (mean value 16%). CNS bound fibronectin (mean value 6%) and type II collagen (mean value 7%), but not fibrinogen (mean value 2%). The specificity of binding of these proteins to S. aureus strain F1440 and to coagulase-negative Staphylococcus chromogenes strain BO52 was studied by adding an excess of unlabelled proteins. Fibronectin and collagen binding were observed to be specific, varying between 50 and 75%, whereas the specificity of fibrinogen binding to S. aureus strain F1440 was lower (26%). Most of the S. aureus strains (63%) showed very high surface hydrophobicity (autoaggregation) or lower hydrophobicity (29% of the strains) and the rest were hydrophilic. None of the CNS strains autoaggregated, 44% were classified as hydrophilic strains. Hydrophilic strains (except the reference strains) did not show extracellular capsule production. However, the encapsulated (reference) strains showed low binding to these proteins as compared to their unencapsulated variants. Pre-treatment of S. aureus strain F1440 and S. chromogenes strain BO52 with trypsin decreased their fibronectin binding capacity and surface hydrophobicity, whereas pre-treatment with bovine milk (except on collagen binding to strain F1440) did not significantly affect binding to these proteins. These data indicate that S. aureus and CNS isolated from bovine udder infection have the ability to bind to tissue matrix and plasma proteins which may be exposed in the traumatized or toxin-damaged udder epithelial lesions.  相似文献   

The relationship between water intake rates,paddy ponding depth,and farmers’ water management techniques     

Mitsumasa?AnanEmail author  Kozue?Yuge  Yoshisuke?Nakano  Tamotsu?Funakoshi  Tomokazu?Haraguchi 《Paddy and Water Environment》2004,2(1):11-18

Japanese farmers manage their irrigation water based on their past experiences and preferences, considering such factors as weather and available water (hereafter defined as empirical water management). They elaborately control the intake and drainage rates of their own paddy fields to maintain optimal ponding depths. But these well-managed systems will drastically change because of the decreasing number of farmers. Therefore, it is necessary to clarify if the optimal ponding depth will be maintained within the limits of traditionally-allowed water intake rate from the main river. The first objective of this study was the quantification of actual water use in the paddy fields, resulting from the farmers water management on the basis of their experience. The significance of the present water intake rate under empirical water management was studied for a paddy field command area of about 230 ha. Water intake rates and the water requirements of the whole area were investigated by measuring the flow rate at 17 points of irrigation and drainage canals. Characteristics of the farmers empirical water management were investigated by measuring the hourly changes in inflow and outflow rates for a sub-area using an automatic measurement system, and an inferential method of determining water management patterns for the paddy fields was proposed. The newly-proposed inferential method was introduced in the tank model, which expresses the characteristics of water management in the command area. The Shuffled Complex Evolution Algorithm (SCE-UA) method was used for optimizing the model parameters. It was proven that the model accuracy improved when the farmers empirical water management was taken into account. The optimal amount of water to be applied to the command area was quantified by the simulation. The second objective was to predict the effect of the decreasing number of farmers on future water use conditions. The simulated result indicates the difficulty of maintaining optimal ponding depth for the whole command area when the farmers empirical water management is not maintained. In other words, results indicated that efficient water use requires an automatic water management system or a new pipeline system to replace the farmers present empirical water management.  相似文献   

Expression of gonadotropin genes in Manchurian trout <Emphasis Type="Italic">Brachymystax lenok</Emphasis> and production of recombinant gonadotropins     

Eunju?Choi  Hyeyeon?Ko  Jihye?Shin  Mi-Ae?Kim  Young?Chang?SohnEmail author 《Fisheries Science》2005,71(6):1193-1200

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The effects of less-expensive plant protein sources replaced with soybean meal in the juvenile diet of grass carp (Ctenopharyngodon idella): growth, nutrient utilization and body composition     

Kenan K?prücü  Ebru Sertel 《Aquaculture International》2012,20(3):399-412

In this study, the effects of less-expensive protein sources replaced with soybean meal on growth performance, nutrient utilization and body composition of juvenile grass carp were investigated. For this purpose, a control diet containing 42% soybean meal was prepared. Cotton seed meal (CSM), sunflower meal (SFM) and corn meal (CM) were added to the experimental diets by replacing 100% of the soybean meal protein used in the control diet. All diets were iso-nitrogenous (40% crude protein) and iso-caloric (19.5?MJ?kg^?1 gross energy). Each treatment had three replicates, and 20 juvenile grass carps (3.5?±?0.1?g, initial weight) were located in per replicate. The fish were fed to satiety three times in a day during 3?months at 26°C of water temperature in glass aquarium (45 l). The results of the study showed that the weight gain (308.6?C448.6%), specific growth rate (1.56?C1.89%/day), feed conversion rate (1.2?C1.9), protein efficiency ratio (1.32?C2.08), and apparent digestibility coefficients of protein (80.5?C89%) or sum of amino acids (80.5?C88.9%) were not significantly affected by the experimental diets, except diet CM. All of these values obtained for the diet CM were significantly lower than those of fish fed on the control, CSM and SFM diets. However, there were no significant differences between the lipid digestibility of the diets, and crude protein, lipid, and gross energy contents in the whole body of fish in experiments. In conclusion, CSM and SFM diets were highly utilized by juvenile grass carp, but not CM diet. These results combined with the remarkable acceptability of diets containing high levels of plant protein ingredients with identical growth performances of juvenile grass carp show clearly that dietary soybean meal level can be considerably reduced without any adverse consequence in terms of somatic growth and nitrogen utilization.  相似文献   

Effect of Artificial Diets on Growth,Survival and Condition of Adult Cuttlefish, <Emphasis Type="Italic">Sepia officinalis</Emphasis> Linnaeus, 1758     

Pedro ?M.?DominguesEmail author  Paul?F. ?Dimarco  Jose? P.?Andrade  Phillip?G.?Lee 《Aquaculture International》2005,13(5):423-440

The effects of artificial diets on growth and body condition of adult cuttlefish, Sepia officinalis were tested in two experiments. Supplemented prepared diets (fish myofibrillar protein concentrate) were fed during a 30-day and a 21-day experiments. Growth, feeding rate and food conversion of group-reared cuttlefish were analyzed. The first of these experiments tested four artificial diets, made with increasing levels of lysine, on adult cuttlefish. According to the chemical analysis, diets 1–3 had limiting concentrations of lysine and other essential amino acids (compared to mantle composition of the cuttlefish), while diet 4 was the only one where almost all essential amino acids were present in concentrations similar or higher than the ones present in cuttlefish mantle. A second experiment was conducted by isolating 16 adult cuttlefish individually, and feeding them the same four artificial diets, in order to obtain individual data. During Experiment 1, only the diet with the best chemical score (diet 4) produced growth (p < 0.05), with a mean instantaneous growth rate (MIGR) of 0.30% wet body weight (BW) d⁻¹. Similarly, individually reared cuttlefish fed diet 4 produced the highest IGR’s (0.26, 0.38 and 0.48% BW d⁻¹) and grew larger (p < 0.01). Comparison of cuttlefish fed the artificial diets vs. thawed shrimp and unfed cuttlefish indicated that cuttlefish fed the artificial diets were in an intermediate state. Growth rates obtained with the artificial diets (<0.4% BW d⁻¹) were considerably lower compared to natural prey, live or frozen, reported by other authors.  相似文献   

Molecular characterization of microalgae used in aquaculture with biotechnology potential     

Mercedes Alonso  Fátima C. Lago  Juan M. Vieites  Montserrat Espi?eira 《Aquaculture International》2012,20(5):847-857

Microalgae are the main component of first tropic level in aquatic food chain; it is for this reason that they are used as food in aquaculture. Also due to its biotechnological potential properties, they are used in the production of diverse components, dyes, antioxidants, enzymes, emulsifiers, etc. The extended ways of microalgae applications require physiologically and genetically stable cultures as well as correctly identified organisms to guarantee reproducibility and reliability. But the variety of species and the morphological similarity between some of them make difficult the identification of some microalgae. The use of molecular markers has supplied a very useful tool for identification of microalgae in fast mode, such as in classification. The present study has worked on the molecular characterization of main species of microalgae used in aquaculture in base of the molecular markers 18S rRNA and 16S rRNA. Microalgae DNA has been amplified and sequenced, and the resultant sequences were analyzed and reflected in phylogenetic trees. The phylogenetic analyses obtained reflect as both molecular markers allow to differentiate the main genus used in aquaculture.  相似文献