控制对虾弧菌病药物的实验研究   总被引：15，自引：0，他引：15  

郑天凌  陈瑛  李福东  蔡立哲 《水产学报》1994,18(3):214-220

研究了一株对虾弧菌病病原菌对２４种药物的敏感性，测定了几种抗菌性较强、抗菌谱较广且价格较低廉的药物对该菌的最低抑菌浓度（ＭＩＣ）和最低杀菌浓度（ＭＢＣ）以及药物在海水环境中对该菌作用的有效性和药物联用效果。结果表明，不同药物具有不同的ＭＩＣ值，在特定的实验条件下，药物之间的作用表现为：协同、拮抗和无关三种结果，不同药物在不海水中作用时间不一。本文对药物滥施乱用的后果进行了讨论并提出了效控制病菌、合  相似文献   

利用变温促进罗非鱼生长的研究   总被引：8，自引：1，他引：8  

桂远明  王志余  陈育辉  郑文辉  李凤知 《水产学报》1989,13(4):326-332

在实验室内不同温度条件(恒温28℃、30℃和周期性变温28±4℃、30±4℃)下,分四组饲养罗非鱼,测定其增重、耗氧率、并推算能量同化率和组织生长效率(K_2)。结果表明,变温组均比相应恒温组生长迅速。其中以变温28±4℃组日增重最快,增重百分率最高,其次为变温30±4℃组,恒温28℃组日增重最慢,增重百分率最低。能量同化率和组织生长效率也以变温28±4℃组最高;30±4℃组能量同化率虽较高,但组织生长效率却最低。本文对变温促进罗非鱼生长的机理和其在生产实践中的意义进行了分析与探讨。  相似文献   

塔里木盆地奥陶系碳酸盐岩缝洞集合体定量地震描述与地质建模     

杨鹏飞  张丽娟  郑多明  李国会  罗浩渝  徐雷 《勤云标准版测试》2014,34(2):24-30

缝洞型碳酸盐岩储层多样多期的地质成因和内部强烈的非均质性,使得常规建模手段难以应用于实际工作中。结合地质体特征和地震相,运用聚类分析技术实现缝洞集合体的几何结构建模;针对缝洞型碳酸盐岩测井资料少、储层内部结构复杂和非均质强的特征,将地质统计学地震反演技术用于储层岩相和孔隙度建模工作中,针对塔里木盆地典型区块缝洞集合体建立静态模型,实现其定量地震描述;进一步利用单井与井组内动态数据,修正缝洞体的规模、边界和连通性,研究成果指导了基于缝洞集合体井位的高效部署、高效开发工作。  相似文献   

Influence of dietary nicotinic acid supplementation on lipid metabolism and related gene expression in two distinct broiler breeds of female chickens     

R. R. Jiang  G. P. Zhao  J. P. Zhao  J. L. Chen  M. Q. Zheng  R. R. Liu  J. Wen 《Journal of animal physiology and animal nutrition》2014,98(5):822-829

  相似文献   

防治秋枫灰同缘小叶蝉的药剂筛选     

郑礼飞  李东文  陈志云  王玲  李奕震  张纯 《绿色科技》2014,(9)

指出了灰同缘小叶蝉是秋枫的重要害虫,利用8种农药室内药效试验显示:30%氯胺磷乳油3000倍液和20%叶蝉散乳油3000倍液对灰同缘小叶蝉成虫和若虫的杀虫效果均达100%,3%啶虫脒乳油、40%乐果乳油、4.5%高效氯氰菊酯乳油和10%吡虫啉可湿性粉剂1000倍液对老熟若虫的杀虫率分别为95.4%、90.8%、81.6%和74.6%;而1.8%阿维菌素乳油和0.5%甲维盐微乳剂3000倍液的杀虫效果较差。  相似文献   

玉米细菌性枯萎病和玉米内州萎焉病的酶联免疫—实时荧光PCR检测     

陈曦  张明哲  林晓佳  吴志毅  陈吴健  吴蓉  夏拯 《浙江农业学报》2014,(5)

针对玉米细菌性枯萎病和玉米内州萎焉病这两种检疫性病菌进行酶联免疫—实时荧光PCR检测研究,对该方法进行了特异性、灵敏度以及实际样品模拟试验。结果表明,该方法具有较好的特异性,检测低限在100~200 cfu·mL-1,同时可用于实际样品的检测鉴定。  相似文献   

不同土壤类型对毛竹林生长的影响研究   总被引：1，自引：1，他引：0  

郑德华 《世界竹藤通讯》2014,(5):32-34

研究了福建省常见的7种土壤类型（红壤、黄红壤、黄壤、紫色土、红土、黄泥土、水稻土）对毛竹林出笋数、新竹成竹率和新竹胸径的影响。结果显示,毛竹林出笋数及新竹胸径在不同土壤类型间均存在极显著差异,新竹成竹率在不同土壤类型处理间的差异不显著。研究结果认为,在毛竹造林时应优先选择退耕的农地,即耕作土壤黄泥土和红土。  相似文献   

基于SPH算法的平面刀土壤切削过程模拟   总被引：6，自引：0，他引：6  

卢彩云  何进  李洪文  王庆杰  郑智旗  张祥彩 《农业机械学报》2014,45(8):134-139

以我国华北一年两熟区保护性耕作地土壤为原型,利用ANSYS/LS_DYNA对平面刀切削土壤过程进行数值模拟,并通过理论分析和试验,验证SPH算法在模拟平面刀切削土壤过程方面的可行性。结合MAT147土壤材料模型,SPH算法及点-面侵蚀接触,运用ANSYS/LS_DYNA软件对平面刀切削土壤过程进行有限元分析。仿真结果表明,SPH算法能够直观模拟平面刀切削土壤整个过程,最大等效应力为5.851 MPa,主要集中在与平面刀接触的土壤上;平面刀切削全过程表明,土壤所受等效应力波动较小,切削过程比较平稳;稳定切削时切削功耗在10.2 kW附近波动,通过理论和试验验证,仿真切削误差不大于0.05。由此说明SPH算法进行平面刀切削过程的数值模拟是可行的。  相似文献   

Alternative vegetable lipid sources in diets for grouper, Epinephelus coioides (Hamilton): effects on growth, and muscle and liver fatty acid composition     

Hei-Zhao Lin  Yong-Jian Liu  Jian-Guo He  Wen-Hui Zheng  & Li-Xia Tian 《Aquaculture Research》2007,38(15):1605-1611

The aim of this study was to investigate the effects of different oils on growth performance and lipid metabolism of the grouper, Epinephelus coioides. Five experimental fish meal‐based isonitrogenous and isolipidic diets were formulated containing either 5.5%‐added fish oil (FO), soybean oil (SBO), corn oil (CO), sunflower oil (SFO) or peanut oil (PO). Each diet was fed to triplicate groups of 20 fish (initial body weight 13.2±0.02 g) grown in seawater at 28.0–30.5 °C for 8 weeks. Fish were fed twice a day to visual satiety. No significant differences in the survival, weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio or hepatosomatic index were found between fish fed the FO or vegetable oils (VO) diets. Dietary lipid sources did not affect whole‐body composition among grouper fed the various diets. Muscle of fish fed the FO diet had significantly higher levels of 14:0, 16:0, 16:1n‐7, 20:5n‐3[eicosapentaenoic acid (EPA)] and docosahexaenoic acid (DHA)+EPA (except for PO fed fish) compared with those of fish fed VO diets. However, the levels of 18:1n‐9, 18:2n‐6 and DHA/EPA ratios in the muscle of fish fed FO diet were significantly lower than those of fish fed the VO diets. The liver of fish fed the FO diet had significantly higher levels of 18:0, 20:5n‐3, 22:6n‐3, n‐3 highly unsaturated fatty acids and DHA+EPA than those of fish fed the VO diets, whereas increases in 18:1n‐9, 18:2n‐6 and mono‐unsaturated fatty acid levels were observed in the liver of fish fed the VO diets.  相似文献   

Study on the distribution and expression of a DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)     

Feng-Rong Zheng  Hong-Zhan Liu 《Aquaculture (Amsterdam, Netherlands)》2006,261(4):1128-1134

The distribution and expression of lymphocystis disease virus (LCDV) vaccine, on the basis of DNA vaccine (pEGFP-N2-LCDV0.6 kb) construction, were analyzed in tissues of the Japanese flounder by PCR, RT-PCR and fluorescent microscopy. Results from PCR studies indicated that the vaccine-containing plasmids were distributed in injected muscle, muscle located opposite the injection site, hind intestine, gill, spleen, head kidney, liver and gonad 7 days after vaccination. However, these vaccine-containing plasmids disappeared by 90 days following vaccination. Fluorescent microscopy observations revealed that green fluorescence appeared in muscle, muscle located at the opposite side of the injection site, hind intestine, gill, spleen, head kidney and liver of fish 36 h after vaccination, and that green fluorescence did not appear in control tissue. The green fluorescence became weaker at 60 days post-vaccination, however, it remained detectable in the spleen 90 days post-vaccination. Results from RT-PCR studies indicated that the Mcp gene is expressed in all tissues of vaccinated fish 7-20 days after vaccination. These results demonstrate that the DNA vaccine is distributed and expressed in different tissues of vaccinated fish, and therefore, may have provided an antigen producing specific immune response.  相似文献