Eastern equine encephalitis in a flock of emus (Dromaius novaehollandiae).     

T N Tully  S M Shane  R P Poston  J J England  C C Vice  D Y Cho  B Panigrahy 《Avian diseases》1992,36(3):808-812

Eastern equine encephalitis (EEE) was diagnosed in a flock of emus in southeastern Louisiana. The outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. The diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of EEE antibody in two recovered emus. High mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. On postmortem examination, hemorrhagic enteritis and multiple petechia of viscera were observed. Microscopic changes included severe necrosis of hepatocytes, intestinal mucosa, and necrotizing vasculitis of the spleen and lamina propria of the intestine. No nervous system lesions were observed. This outbreak occurred concurrently with EEE in horses and was attributed to unseasonably heavy rainfall with an abundance of arthropod vectors and proximity to free-living reservoir host species.  相似文献   

斑点免疫吸附试验诊断羊脑多头蚴病的研究     

姚新华  周维等 《吉林畜牧兽医》2001,(12):3-5

以原头节可溶性粗抗原经Sephadex G-200层析纯化抗原为包被抗原，兔抗羊IgG-HRP结合物为显色剂，建立检测羊脑多头蚴病血清抗体的Dot-ELISA，并以ELISA作平行对照。结果，粗抗原和层析纯化抗原检测86头份羊脑多头蚴病阳性血清，其敏感性分别为94．18％和93．02％，两种抗原的敏感性无显著差异；检测122头份绦虫蚴病阴性血清，18头份棘球蚴病阳性血清，35头份细颈囊尾蚴病阳性血清，其特异性分别为90．29％和95．43％。两种抗原的特异性差异显著。Dot-ELISA和ELISA两种方法的符合率为100％。层析纯化抗原比粗抗原的特异性有了明显提高，而敏感性没有降低。层析纯化抗原和操作术式都具有良好的重复性，Dot-ELISA和ELISA对比试验结果相近，且具简便、快速及不需要复杂设备等优点，是一种检测羊脑多头蚴病血清抗体的理想方法。  相似文献   

剌槐种子萌发过程的微量热法研究     

胡云楚  周培疆 《种子》1994,(6):16-17,59

用微量热法测定了剌槐种子的萌发热谱，发现其产热规律与其吸水规律和呼吸规律相关，并获得了衡量种子活力４个参数。  相似文献   

Effect of dietary vitamin A on resistance to experimental Escherichia coli infection of uterus in rats     

T Inaba  A Inoue  Y Nakano  J Mori 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(6):1247-1249

  相似文献   

Toxic peripheral neuropathy with demyelination in Sprague-Dawley rats given CGS 21595--a 5-lipoxygenase inhibitor.     

D E Gunson  P S Sahota  W O Iverson  R Y Chau  G M McCormick  V M Traina 《Veterinary pathology》1992,29(2):145-151

Male and female Sprague-Dawley rats were given CGS 21595, a pro-drug that is almost immediately metabolized to CGS 19213, a naphthoquinone that acts as a 5-lipoxygenase inhibitor. The compound was administered by gavage to five groups of Sprague-Dawley rats (group Nos. 1, 5, n = 30; group Nos. 2-4, n = 20) at daily doses of 0, 50, 150, 500, or 1,000 mg/kg for 13 weeks. Rats in the higher dose groups had a reduced weight gain, but significant neurologic signs were not observed. A peripheral neuropathy consisting predominantly of myelin destruction in the spinal nerve roots and sciatic nerves in male rats treated with greater than or equal to 150 mg/kg CGS 21595 and in female rats treated with greater than or equal to 50 mg/kg CGS 21595 for 13 weeks. This lesion was not fully reversible after a recovery period of 4 weeks. Lesions consisted of ballooning of myelin sheaths, infiltration by macrophages, demyelination, and occasional areas of remyelination. Axons were generally preserved, and the brain and spinal cord were not affected. Male and female rats in all treatment groups had cytoplasmic hyaline droplets in the proximal renal tubules. This change was reversible after 4 weeks and was not associated with any other adverse effects on the kidney.  相似文献   

心脏体外循环系统检测植物外啡肽活性的研究     

孙镇平  华棣  吕立力  熊万斌  周业飞 《猪业科学》2003,20(7):71-72

本文利用猪胃蛋白酶在37℃条件下降解中筋粉,采集不同反应时间的酶解液样品,低温离心处理后,分别加入蛙心体外循环系统中,测定心肌收缩力变化,并随即加入阿片肽阻断剂-纳洛酮,检测植物外啡肽活性。结果表明,酶解液能够引起心肌收缩力加强,并可被纳洛酮逆转,证明酶解液具有植物外啡肽活性。  相似文献   

胃肠道线虫的黏膜免疫     

韩周路义鑫  宋铭忻 《中国兽医科技》2006,36(8):679-683

从机体抗胃肠道线虫的黏膜免疫、胃肠道黏膜的抗原加工与递呈、胃肠道黏膜抵御寄生虫的效应机制等几方面论述了胃肠道线虫的免疫机理和免疫学研究的进展，旨在进一步阐明用免疫介入方法防治胃肠道线虫的理论基础，以解决胃肠道线虫治疗过程中的药物残留以及寄生虫的抗药性问题。  相似文献   

Manipulated Mouse Embryos as Bioassay System for Water Quality Control   总被引：1，自引：0，他引：1  

AS Elsheikh  Y Takahashi  M Nagano  H Kanagawa 《Reproduction in domestic animals》2003,38(3):204-208

Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K⁺ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system.  相似文献   

浅析防火墙对网络安全的作用     

周翘宇 《林业科技情报》2006,38(1):64-65

文中就信息网络安全内涵发生的根本变化,论述了网络防火墙安全技术的分类及其主要技术特征。  相似文献   

内2优6号生育特性及高产栽培技术模式研究     

周祖昌奚天位  张玉屏曹雪仙鲁贤平  朱德峰 《勤云标准版测试》2005,(9)

内2优6号全生育期146d,适于单季稻种植,分蘖力中等,株型较大,每穗约200粒,粒重高,千粒重30.5g,抗倒性较强。但叶片较长较宽。高产栽培的关键,培育壮苗,扩行稀植,控制中后期氮肥用量,增施钾肥,浅湿干灌溉,促进根系生长,提高根系活力和结实率。  相似文献