荷叶总生物碱降脂减肥作用的体内外试验     

范婷婷  法鲁克  方芳  蒋益虹 《浙江大学学报(农业与生命科学版)》2013,(2):141-148

通过体内、体外试验探究荷叶总生物碱的降脂减肥作用.给予高脂血症大鼠模型组不同剂量荷叶总生物碱和降脂药物辛伐他汀(阳性药物对照组),观察荷叶总生物碱对高脂血症大鼠体质量、体脂以及血清脂质相关指标的影响;用对硝基苯酚法检测不同质量浓度荷叶总生物碱处理对大鼠胰脂肪酶活性的影响.结果显示:荷叶总生物碱能显著降低高脂血症大鼠的体质量,显著降低血清总胆固醇、血清甘油三酯、低密度脂蛋白胆固醇含量,显著降低谷草转氨酶、谷丙转氨酶活性以及动脉粥样系数,升高高密度脂蛋白胆固醇/血清总胆固醇比值;荷叶总生物碱能抑制脂肪酶活性,抑制类型为非竞争性抑制;在荷叶总生物碱质量浓度为12.5～600μg/mL范围内,对脂肪酶的抑制效果随其质量浓度的增加而增强,在600μg/mL时达到最大抑制率,为25.6%,当荷叶总生物碱质量浓度继续增加时,抑制率趋于平缓,保持在最大值附近.体内、体外试验结果均表明,荷叶总生物碱类物质具有降脂减肥作用,提示其可能具有预防肥胖、调节脂代谢紊乱的功效.  相似文献   

黄土藻类回接之生境条件研究   总被引：1，自引：0，他引：1  

许永霞  廖超英  孙长忠  法蕾  马淑敏 《西北林学院学报》2012,(2):30-33

通过液体藻种固相接种培养试验,对人工培养藻类结皮的生态环境条件进行了研究,探讨了温度、光照、水分对藻类生长繁殖的影响规律。结果表明:温度条件对藻类的生长有影响,20℃和室温条件下藻类生物量平均增长率较小,说明其生长比35℃条件下稳定;从光照条件来看,全光和暗光条件下藻类生物量逐渐减少,而弱光条件下生物量持续增加,说明弱光条件下藻类生长最旺盛;水分是影响藻类生长的重要条件,土壤含水量达到30%～35%时藻类生长良好,说明足够的水分供给能够增强液体藻种对环境的适应性,因此,在藻类接种的初期,加强水分的供应能够保证人工培养藻类结皮的成活率。  相似文献   

Neonicotinoids and bumblebees (Bombus terrestris): effects on nectar consumption in individual workers          下载免费PDF全文

Helen M Thompson  Selwyn Wilkins  Sarah Harkin  Sarah Milner  Keith FA Walters 《Pest management science》2015,71(7):946-950

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Summer Anoestrus in Buffalo – A Review     

GK Das  FA Khan 《Reproduction in domestic animals》2010,45(6):e483-e494

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Recent advances in computational fluid dynamics relevant to the modelling of pesticide flow on leaf surfaces     

C Richard Glass  Keith FA Walters  Philip H Gaskell  Yeaw C Lee  Harvey M Thompson  David R Emerson  Xiao‐Jun Gu 《Pest management science》2010,66(1):2-9

Increasing societal and governmental concern about the worldwide use of chemical pesticides is now providing strong drivers towards maximising the efficiency of pesticide utilisation and the development of alternative control techniques. There is growing recognition that the ultimate goal of achieving efficient and sustainable pesticide usage will require greater understanding of the fluid mechanical mechanisms governing the delivery to, and spreading of, pesticide droplets on target surfaces such as leaves. This has led to increasing use of computational fluid dynamics (CFD) as an important component of efficient process design with regard to pesticide delivery to the leaf surface. This perspective highlights recent advances in CFD methods for droplet spreading and film flows, which have the potential to provide accurate, predictive models for pesticide flow on leaf surfaces, and which can take account of each of the key influences of surface topography and chemistry, initial spray deposition conditions, evaporation and multiple droplet spreading interactions. The mathematical framework of these CFD methods is described briefly, and a series of new flow simulation results relevant to pesticide flows over foliage is provided. The potential benefits of employing CFD for practical process design are also discussed briefly. © Crown copyright 2009. Reproduced with permission of Her Majesty's Stationery Office. Published by John Wiley & Sons, Ltd.  相似文献   

Aspergillosis in camels affected with a specific respiratory and enteric syndrome     

A-BA EL-KHOULY  FA GADIR  DD CLUER†  GW MANEFIELD† 《Australian veterinary journal》1992,69(8):182-186

During the summer of 1990 deaths, occurred in racing camels (Camelus dromedarius) associated with a specific disease syndrome. Clinical signs included pyrexia, coughing, lachrymation, oedema of the throat and submandibular region and enlargement of submandibular lymph nodes. In terminal cases nervous signs were present and sometimes there was bloody diarrhoea and vomiting. Of 480 camels at least 70 animals were affected with the disease and about 40 died. Morbidity and mortality was greater in camels recently imported. Consistent necropsy findings were extensive petechial and ecchymotic haemorrhage beneath the epicardium, endocardium and visceral pleura and in the mediastinal lymph nodes, and haemorrhagic oedema of the pharyngeal and laryngeal areas. Haemorrhages occurred more variably in abdominal organs and on the omasal and abomasal mucosa. Bronchopneumonia, omasitis and abomasitis were observed on microscopic examination, together with liver and kidney lesions of presumed toxic origin. Fungal hyphae and, occasionally, the characteristic conidial morphology of Aspergillus fumigatus were seen in sections and direct smears from lesions in the respiratory and alimentary tracts. A fumigatus was cultured from trachea, bronchi, bronchioles, lung tissue, heart blood, omasum, abomasum, ileum and submandibular lymph nodes. Whether the role of Aspergillus in the overall syndrome is primary or secondary has not been established; no other potential aetiological agent has been identified.  相似文献   

Sensitivity of the standardized pseudorabies virus neutralization test varies with the test strain used.     

G Scherba  R M Weigel  L Jin  W Hall  F A Zuckermann 《Journal of veterinary diagnostic investigation》1991,3(4):306-312

The effect of altering the strain of the test virus used in the standardized pseudorabies virus neutralization (VN) test on the sensitivity of the assay was evaluated. Comparative VN tests were performed using 4 different strains: the avirulent Bartha parental, the avirulent recombinant Bartha gIIIKa, the moderately virulent Shope (currently used for the VN test at the National Veterinary Services Laboratory, Ames, IA), and the highly virulent P2208 (Funkhauser). A radioimmunoassay and a Western immunoblotting technique were employed to verify the presence of anti-pseudorabies virus (PrV) antibodies in sera. Statistical analysis indicated that replacement of the Shope strain by the Bartha gIIIKa or the P2208 strain resulted in VN titers that were 4.23- and 2.00-fold higher, respectively. Despite these differences, specificity with regard to PrV diagnosis was unaltered. This apparent enhancement of the sensitivity of the PrV VN test would be beneficial for the serologic identification of PrV-infected animals during an eradication effort.  相似文献   

Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy   总被引：1，自引：0，他引：1  

Keppel KE  Campbell KL  Zuckermann FA  Greeley EA  Schaeffer DJ  Husmann RJ 《Veterinary immunology and immunopathology》2008,123(3-4):337-344

Canine atopic dermatitis (AD) shares many clinical and immunological similarities with human AD. Regulatory T cells (Treg) are a distinct lineage of T lymphocytes with various immunosuppressive properties including the down-regulation of allergic inflammation associated with IgE production. Antigen-induced Treg typically regulate immune homeostasis via productions of cytokines such as interleukin-10. Given the immunological similarities with human AD, it is likely that Tregs and the cytokines they produce play an important role in diseases of dogs as well. A cross-reactive FoxP3 antibody was used to identify a subset of CD4(+) T cells in the blood of both healthy dogs and dogs with atopic dermatitis undergoing immunotherapy over a year period. There was no significant difference in the Treg percentage over time in the healthy dogs. The immunotherapy group showed a significant increase in Treg percentage at 6, 9, and 12 months when compared to the healthy dogs. For the immunotherapy group, the mean Treg percentage at the beginning of the study was 4.94+/-0.71 and 10.86+/-2.73 at the completion. A commercially available ELISA kit was also used to quantitate the concentration of IL-10 in the serum of the same subsets of dogs. There was no significant difference in the IL-10 concentrations over time in the healthy dogs. The immunotherapy group showed a significant increase in serum IL-10 concentrations at 6, 9, and 12 months when compared to the control group. The mean serum IL-10 concentration at the initiation of immunotherapy was 20.40+/-3.52ngL(-1) and 37.26+/-15.26ngL(-1) at the completion of the study. The immunotherapy group also showed a significant decrease in serum IgE levels over the 1-year treatment period for specific allergens identified during ASIT. We conclude from these studies that similar to humans undergoing immunotherapy, increasing Treg populations likely play a significant role in the success of this particular type of therapy for atopic dermatitis and other allergic conditions.  相似文献   

Enrichment of Y‐Chromosome‐Bearing Bull Spermatozoa by Swim‐up Through a Column     

A Azizeddin  FA Ashkar  WA King  T Revay 《Reproduction in domestic animals》2014,49(1):e1-e4

With the advancement of assisted reproductive biotechnologies, preselecting the sex of offspring has become an important goal for cattle and other livestock breeding as well as for research. The aim of this study was to investigate the feasibility of producing enriched pools of X‐ or Y‐chromosome‐bearing sperm by vertical swim‐up through a long, narrow column. Sperm recovered from the top portion of the column was predominantly Y‐bearing (60%, p < 0.05), which were capable of fertilizing matured oocytes and produce significantly more male embryos compared with standard swim‐up protocol.  相似文献   

安徽特色农业可持续发展研究     

叶良均  法剑 《安徽农业大学学报》2014,(3):1-8；32

当前安徽特色农业呈不断发展趋势：专业化和产业化不断增强、基础设施逐步改善、科技扶持力度不断增强、农业旅游等功能有所拓展。但是也存在优良品种保护和开发力度不够,生产技术水平相对较低,基层农技推广和农村实用人才难以满足需求,基础设施建设投入不足,主产区生态环境保护不够,产业化有待推进,休闲、观光旅游功能拓展缓慢等问题。建议从五个方面推进安徽特色农业可持续发展：加强基层农业科技人才开发;推进特色农业产业化发展;加强优良品种开发与保护;完善农业基础设施建设;保护生态环境,发展农业旅游。  相似文献