Occurrence of tumorous form of bovine leucosis]     

R B?ke 《Tier?rztliche Praxis》1975,3(2):167-172

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Effect of taurine supplemented practical diet on growth performance and taurine contents in whole body and tissues of juvenile Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>     

Shin-Kwon?Kim  Toshio?TakeuchiEmail author  Atsushi?Akimoto  Hirofumi?Furuita  Takeshi?Yamamoto  Masahito?Yokoyama  Yuko?Murata 《Fisheries Science》2005,71(3):627-632

ABSTRACT:   The effect of dietary taurine on juvenile Japanese flounder was determined by feeding three taurine-supplemented experimental diets (TAU) and a commercial diet (CD) to evaluate a practical diet for juvenile Japanese flounder. Juvenile Japanese flounder were reared on the three experimental diets supplemented with taurine at 0, 0.5, 1.0% and CD. These diets were fed to juvenile Japanese flounder of an initial mean body weight of 0.2 g for 6 weeks at 20°C and the taurine contents of the whole body and tissues were analyzed. The final average body weight of juvenile Japanese flounder fed the 1.0% TAU was significantly higher than that of the other groups. Taurine contents in the whole body and tissues increased with the increase in dietary taurine level. These results indicate that juvenile Japanese flounder require at least 15 mg/g taurine in the diet, even though a combined mix of fish, krill and squid meal was the main protein source in the experimental diets.  相似文献   

Comparative study on thermal denaturation modes of myosin in walleye pollack and carp myofibrils as affected by salt concentration     

Masayuki?Takahashi  Takeshi?Yamamoto  Sanae?Kato  Kunihiko?KonnoEmail author 《Fisheries Science》2005,71(3):662-671

ABSTRACT:   The effect of salt concentration on the thermal denaturation profile of myosin in walleye pollack and carp myofibrils was compared by studying the subfragment-1 (S-1) and rod denaturation rates upon heating. Species-specific denaturation mode observed at 0.1 M KCl was no longer detected when samples were heated above 0.5 M KCl, where S-1 and rod denaturation rates were identical to each other. As the heating of the chymotryptic digest of myofibril formed practically no rod aggregates, S-1 denaturation in a form of myosin was the rate limiting step for rod aggregate formation. As the aggregate formation by rod was remarkably suppressed by lowering the temperature, the free movement of myosin tail upon heating was suggested to play an important role in the rod aggregate formation in a high salt medium.  相似文献   

Rat Ghrelin Stimulates GH Release and GH mRNA Expressionin the Pituitary of Orange-spotted Grouper, <Emphasis Type="BoldItalic">Epinephelus Coioides</Emphasis>     

Xueqin?Ran  Wensheng?Li  Haoran?LinEmail author 《Fish physiology and biochemistry》2004,30(2):95-102

Ghrelin was recently demonstrated as an endogenous ligand of the growth hormone (GH) secretagogue receptor (GHS-R), which could promote the release of GH in mammal significantly. The present study conducted to determine whether ghrelin stimulate the release and synthesis of GH in orange-spotted grouper (Epinephelus coioides). Rat ghrelin was incubated with the pituitary fragments of grouper in static culture system. The culture medium was collected at 1, 6, 12, 18 and 24 h after incubation to detect the contents of GH by homologous radioimmunoassay. The level of GH mRNA in the pituitary fragments was measured by a sensitive chemiluminescent ribonuclease protection assay. The results showed that rat ghrelin not only stimulated the release of GH but also augmented the GH mRNA level in grouper. It suggested that the ghrelin-like peptide and the GHS-R involved in the regulation of GH synthesis and release in grouper. The present study would provide a better understanding of the regulatory mechanism of GH release in marine fish.  相似文献   

Molecular cloning and expression of insulin-like growth factor-I (IGF-I) in Eurasian perch (<Emphasis Type="Italic">Perca fluviatilis</Emphasis>): lack of responsiveness to growth hormone treatment     

Sissel?JentoftEmail author  Are?H.?Aastveit  ?ivind?Andersen 《Fish physiology and biochemistry》2004,30(1):67-76

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Are Primary Cultures of Trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) Ventricular Cardiomyocytes Metabolically Viable?     

J.P?Goldstein  A.?Scott  S.?CurrieEmail author 《Fish physiology and biochemistry》2004,30(2):109-117

The objectives of this study were to establish a protocol for the isolation of metabolically viable ventricular cardiomyocytes from the rainbow trout and to determine which measures may best reflect viability 24 h after isolation. Cardiomyocytes were isolated by enzymatic digestion and maintained in cell suspension. Viability was assessed using Trypan blue dye exclusion, ATP content, oxygen consumption and lactate dehydrogenase (LDH) leakage into the medium. Viability, assessed by these measures did not significantly change over the time period of this study. ATP content did correlate significantly with oxygen consumption but not with Trypan blue exclusion. We conclude that primary cultured cardiomyocytes remain metabolically viable for at least 24 h after isolation. Also, it appears that ATP content and oxygen consumption most adequately reflect metabolic cell viability. To be confident with a culture, however, a combination of viability measures is necessary when isolating cardiomyocytes from fish.  相似文献   

Treatment Method Affects Color,Chemical, and Mineral Composition of Seabream (Sparus aurata) Fish Bone Powder from by-Products of Fish Fillet     

Nazlı Savlak  Özlem Çağındı  Gizem Erk  Birsen Öktem  Ergün Köse 《Journal Of Aquatic Food Product Technology》2020,29(6):592-602

ABSTRACT

Seabream fish bone powder was produced using different chemical methods and tap water. The effect of different chemical procedures (sodium hydroxide (NaOH); NaOH + citric acid; NaOH + sodium hypochlorite (NaOCl); NaOH + ethanol (EtOH); and NaOH + hydrogen chloride (HCl)) and tap water on proximate composition, yield, mineral composition, color, and sensorial odor was investigated. Despite its high bone powder yield (59.39%), tap water treatment was not an efficient method due to low calcium (Ca²⁺, 232.13 g/kg) and phosphorus (P, 111.63 g/kg) concentration and heavy fish odor. Sensorial odor analysis of seabream fish bone powder showed that tap water received the lowest scores (1.71/5), while chemically treated samples received sensorial odor scores higher than 4.00, with an average of 4.61, indicating that they had a very slight odor. The best fish bone powder yield was obtained using NaOH treatment (21.46%), where Ca²⁺ (276.73 g/kg) and P (147.23 g/kg) content was also high. The utilization of chemicals in combination with NaOH did not increase the sensorial odor score of seabream fish powders but resulted in a decrease in powder yield. Moreover, the mineral composition of all chemical processing techniques was comparable. Processing by-products of seabream fillet production with 8% NaOH will contribute to daily Ca²⁺ and P intake of individuals.  相似文献   

Emissions of CO₂, CH₄ and N₂O from Southern European peatlands     

Tjaša Danev?i?  Bla? Stres  David Stopar  Janez Hacin 《Soil biology & biochemistry》2010,42(9):1437-1446

Peatlands play an important role in emissions of the greenhouse gases CO₂, CH₄ and N₂O, which are produced during mineralization of the peat organic matter. To examine the influence of soil type (fen, bog soil) and environmental factors (temperature, groundwater level), emission of CO₂, CH₄ and N₂O and soil temperature and groundwater level were measured weekly or biweekly in loco over a one-year period at four sites located in Ljubljana Marsh, Slovenia using the static chamber technique. The study involved two fen and two bog soils differing in organic carbon and nitrogen content, pH, bulk density, water holding capacity and groundwater level. The lowest CO₂ fluxes occurred during the winter, fluxes of N₂O were highest during summer and early spring (February, March) and fluxes of CH₄ were highest during autumn. The temporal variation in CO₂ fluxes could be explained by seasonal temperature variations, whereas CH₄ and N₂O fluxes could be correlated to groundwater level and soil carbon content. The experimental sites were net sources of measured greenhouse gases except for the drained bog site, which was a net sink of CH₄. The mean fluxes of CO₂ ranged between 139 mg m⁻² h⁻¹ in the undrained bog and 206 mg m⁻² h⁻¹ in the drained fen; mean fluxes of CH₄ were between −0.04 mg m⁻² h⁻¹ in the drained bog and 0.05 mg m⁻² h⁻¹ in the drained fen; and mean fluxes of N₂O were between 0.43 mg m⁻² h⁻¹ in the drained fen and 1.03 mg m⁻² h⁻¹ in the drained bog. These results indicate that the examined peatlands emit similar amounts of CO₂ and CH₄ to peatlands in Central and Northern Europe and significantly higher amounts of N₂O.  相似文献   

Stabilization of Mine Tailings Using Fly Ash and Sewage Sludge Planted with Phalaris arundinacea L.     

Clara Neuschütz  Maria Greger 《Water, air, and soil pollution》2010,207(1-4):357-367

The impact of plants (Phalaris arundinacea L.) on the leakage of ammonium, cadmium, copper, nitrate, phosphate, and zinc from sulfidic mine tailings covered with wood fly ash and sewage sludge was investigated. Either ash or sludge was placed in contact with the tailings, and ash layers of either low or high compactness were used. It was revealed that an ash/sludge cover effectively decreased the metal leaching from the tailings regardless of the order in which the materials were applied. Plants decreased the amount of leachate and the concentrations of ammonium and phosphate. The presence of ash below the sludge decreased the plant uptake of copper and zinc and nitrate leakage. However, when the ash was added as a thin (1.5 cm) porous layer, roots and air reached the tailings and caused high metal leakage. The results support the use of a vegetated ash/sludge cover in the treatment of mine tailings, provided that the sealing layer is firm enough to prevent root penetration.  相似文献   

Literarische Berichte     

Dr. L. Wappes  Schüpfer  Dotzel  Dr. E. 《European Journal of Forest Research》1908,30(11):608-625

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