排序方式： 共有79条查询结果，搜索用时 15 毫秒
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[目的]明确新疆和田地区骏枣果实疮痂病的病原菌。[方法]从新疆和田地区枣园病果上分离患病组织,然后对其进行分离、提纯、致病性测定、形态观察和分子生物学鉴定。[结果]从病果上共分离到2株细菌菌株,该2株细菌在NA培养基上均形成灰白色圆形菌落,菌落具光泽,略隆起。显微镜下细菌菌体短杆状,革兰氏染色反应为阴性。经柯赫氏法则证明均为骏枣疮痂病的致病菌,16SrD—NA序列分析结合形态学鉴定将致病菌鉴定为假单胞菌属（Pseudomonassp．）。[结论]为今后疮痂病病原菌的系统研究和防治提供了理论依据。 相似文献
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[目的]筛选快速繁殖棉花黄萎病菌微菌核的适宜培养基.[方法]用6种培养基培养大丽轮枝菌(Verticillium dahliae Kleb.),通过生长速率、微菌核数量、微菌核直径和微菌核萌发率4项指标筛选合适的培养基,用5株大丽轮枝菌菌株对实验结果进行验证.[结果]改良燕麦培养基形成微菌核的数量和直径均优于其他培养基;M1和BMM培养基形成微菌核的数量较多,其微菌核直径均小于半组合培养基;验证的结果与实验结果基本一致.[结论]改良燕麦、M1和BMM培养基,根据实验需要均可作为快速繁殖微菌核的理想培养基. 相似文献
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新疆首次报道轮枝镰孢菌引起骏枣果实黑点病 总被引：1，自引：0，他引：1
Black spot disease was found on fruits of Jun jujube in Hetian area, Xinjiang, in June,2010. The typical spots on fruits were round or suborbicular, concave slightly,dark brown and the diameter was about 4 mm. The colony grew fast with irregular edge on PSA medium, while relatively slow with smooth edge on PDA medium. The colonies were white at the early stage, slightly pink finally and flocculent on the both culture medium. The conidia arranged chain shape and the spore chain was basipetal. The rDNA ITS sequence,cyt b gene sequence and EF-1α gene sequence were amplified and analyzed. The result showed that the homology was above 99% between the isolate and Fusarium verticillioides strains based on the three DNA sequences. By morphology combining with molecular biology techniques,the pathogen of Jun jujube black spot disease was identified as F. verticillioides. It is the first report on jujube disease caused by F. verticillioides in the world. 相似文献
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