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Bipul Phukan Avinash Talukdar Rinku Kalita Bhargav Bhushan Nath Niti Sharma Ishfaq Nazir Mir Pavan Kumar Annam Sangipran Baishya 《Aquaculture Research》2022,53(1):22-35
The study was to examine the effects of dietary L. aspera on growth performance, physio-metabolic response and health status of Rita rita fish reared in tanks for 60 days. Six experimental diets were formulated containing 0 g/kg, 3 g/kg, 6 g/kg, 9 g/kg and 12 g/kg L. aspera and designated as D0, D3, D6, D9 and D12 respectively. The inclusion of L. aspera at graded levels resulted in significantly varying effects on growth performance where the weight gain (%), specific growth rate (SGR) and feed conversion efficiency (FCE) were significantly higher (p<0.05) in D6 and D9 groups than others. The protease and amylase activities were elevated in L. aspera supplemented groups than the control group (p<0.05), whereas lactate dehydrogenase, malate dehydrogenase and serum transaminase enzyme activities were significantly reduced with the increasing level of L. aspera and comparatively higher activities of the respective enzymes were found in the control group. The activities of oxidative stress enzymes were significantly higher (p<0.05) in the D9 group and lower in control. The blood glucose level significantly decreased with the increasing level of L. aspera in the experimental diets. The L. aspera supplemented fish had significantly higher liver glycogen, serum protein, albumin and globulin than control group. Red blood cell count was significantly higher (p<0.05) in D6-D12, whereas white blood cell count and haemoglobin content were significantly higher (p<0.05) in D6 and D9 groups. Regression analysis showed that L. aspera at the level of 7.3–7.9 g/kg is optimum for better growth and feed efficiency. 相似文献
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Rathore Avinash Chandra Mehta Harsh Islam Sadikul Saroj P. L. Sharma N. K. Jayaprakash J. Gupta A. K. Dubey R. K. Ghosh B. N. Prasad Ram Kumar Dinesh Raizada A. 《Agroforestry Systems》2021,95(8):1563-1575
Agroforestry Systems - This study aims to develop predictive models for standing stocks of biomass and carbon of an entire tree based on harvested biomass in mango (Mangifera indica L.) alongwith... 相似文献
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Semecarpus kurzii Engl. is an important ethnomedicinal plant used for curing tumours, inflammation, fever and pain, and is also a source of iso ricinoleic acid. During the present investigation, effect of chemical pre-treatments on germination and seedling growth revealed significant differences. Different pre-treatments could improve germination and treatment with KNO3 (9.9 mM) was significantly superior (94%) to the untreated control (76%). Mean daily germination varied among the treatments (0.90–4.14 seeds per day), when compared with 2.27 seeds per day in control. Mean germination time was significantly reduced from 22.14 days (control) to 16.40 days (19.8 mM KNO3) and 17.16 days (1.45 mM GA3). Treatment with KNO3 (19.8 mM) and GA3 (1.45 and 2.90 mM) significantly reduced the time for 50% germination. Treatment with higher concentrations of GA3 and KNO3 exhibited significantly faster germination initiation (11.0 and 12.0 days, respectively) over the control (17.0 days). Seedling vigour index was not improved by pre-treatments. Chemical treatments did not improve shoot growth, collar thickness, or number of seedling leaves; however, root growth was significantly improved over control. Treatment with GA3 (2.90 mM) promoted leaf elongation but reduced leaf width significantly. Treatment with KNO3 and GA3 could be recommended to obtain healthy seedlings for establishing new plantations of this important medicinal plant species. 相似文献
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Semecarpus kurzii Engl.is an important ethnomedicinal plant used for curing tumours,inflammation,fever and pain,and is also a source of iso ricinoleic acid.During the present investigation,effect of chemical pretreatments on germination and seedling growth revealed significant differences.Different pre-treatments could improve germination and treatment with KNO_3 (9.9 mM)was significantly superior(94%) to the untreated control(76%).Mean daily germination varied among the treatments(0.90–4.14 seeds per day),when compared with 2.27 seeds per day in control.Mean germination time was significantly reduced from 22.14 days(control) to 16.40 days(19.8 mM KNO_3) and 17.16 days(1.45 mM GA_3).Treatment with KNO_3(19.8 mM) and GA_3(1.45 and 2.90 mM)significantly reduced the time for 50% germination.Treatment with higher concentrations of GA_3 and KNO_3 exhibited significantly faster germination initiation(11.0 and 12.0 days,respectively) over the control(17.0 days).Seedling vigour index was not improved by pre-treatments.Chemical treatments did not improve shoot growth,collar thickness,or number of seedling leaves; however,root growth was significantly improved over control.Treatment with GA_3(2.90 mM) promoted leaf elongation but reduced leaf width significantly.Treatment with KNO_3 and GA_3 could be recommended to obtain healthy seedlings for establishing new plantations of this important medicinal plant species. 相似文献
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Avinash Aujayeb 《The Forestry Chronicle》2013,24(3):295-297
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Salicornia brachiata is an extreme halophyte that grows in salty marshes and is considered to be a potential alternative crop for seawater agriculture. Salicornia seeds are rich in protein, and its tender shoots are eaten as salad greens. Seed storage proteins were fractionated by sequential extraction using different solvents, including distilled water for albumins, NaCl (1.0 M) for globulins, NaOH (0.1 N) for glutelins, and ethanol (70% v/v) for prolamins. Globulins accounted for 54.75% of the total seed storage proteins followed by albumins (34.30%) and glutelins (8.70%). The fractionated proteins were characterized using 2D-diagonal SDS-PAGE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. The globulin fraction, composed of seven intermolecular disulfide-linked polypeptide pairs of molecular mass 63.5, 62.5, 54.7, 53.0, 43.2, 38.5, and 35.1 kDa, encompassed a basic and an acidic subunit. Two-dimensional gels revealed approximately 32 spots, with isoelectric points and molecular masses ranging from 4.93 to 11.6 and from ～5.2 to ～109.4 kDa, respectively. Protein spots were identified by MALDI-TOF MS peptide mass fingerprint analysis and further classified. Homology analysis demonstrated that 19% of the proteins were involved in metabolism, 16% were involved in signaling, and 15% were regulatory proteins. Peptide mass fingerprint analysis confirmed the presence of inter- and intramolecular disulfide linkages in the globulin fraction. Sulfur-rich proteins are of high nutritional value, and disulfides make S. brachiata a potential source of dietary supplementation. 相似文献
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Post-translational modifications like ubiquitination determine the fate of many cellular proteins. There are evidences about the role of these ubiquitinated proteins in the antiviral immune response. E3 ubiquitin ligases like RBR (RING between RING) proteins play a crucial role in the regulation of immune signalling. In the present study, two RBR genes (RNF114 and RNF144A) from Asian seabass (Lates calcarifer) were cloned and characterized. Phylogenetic analysis reveals that these genes were closely clustered with their counterparts from teleost origin. Structurally they feature multiple domains with RING1 and RING2 domains and an IBR (in between RING) domain. The challenge studies with poly(I:C) and RGNNV virus gave evidence that both Lates RNF114 and RNF144A genes play a crucial role in antiviral immune response. Our studies suggest that RBR is an important gene in innate immune response and shed light on antiviral immune research in Lates calcarifer and its aquaculture.
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