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21.
Katsuhiko Yoshizawa Yuichi Kinoshita Yuko Emoto Ayako Kimura Norihisa Uehara Takashi Yuri Nobuaki Shikata Airo Tsubura 《Journal of toxicologic pathology》2013,26(2):141-148
N-Methyl-N-nitrosourea (MNU)-induced renal tumors in rats and Wilms tumors in humans were compared. Renal mesenchymal tumors (RMTs) and nephroblastomas (blastemal and epithelial components) in female Lewis rats treated with a single intraperitoneal injection of 50 mg/kg MNU at birth and Wilms tumors (blastemal, epithelial and mesenchymal components) in humans were analyzed for the expression of pancytokeratin (CK), vimentin, p63, α-smooth muscle actin (SMA), desmin, S-100, CD57, CD117/c-kit, Wilms tumor 1 protein (WT1) and β-catenin. The mesenchymal components of rat RMTs and human Wilms tumors expressed vimentin, SMA and β-catenin. The blastemal components of rat nephroblastomas and human Wilms tumors expressed vimentin, CD117/c-kit and β-catenin. The epithelial components of rat nephroblastomas and human Wilms tumors expressed vimentin and β-catenin. WT1 was expressed in different cellular components of rat tumors as compared with human Wilms tumors; the expression was seen in mesenchymal tumors and blastemal components of nephroblastomas in rats and epithelial components in human Wilms tumors. CK, p63 and CD57 were not expressed in rat RMTs or nephroblastomas, while CK and WT1 were expressed in epithelial components and CD57 was expressed in blastemal and epithelial components of human Wilms tumors. Rat and human tumors were universally negative for the expression of desmin and S-100. The immunohistochemical characteristics of rat renal tumors and human Wilms tumors may provide valuable information on the differences in renal oncogenesis and biology between the two species. 相似文献
22.
Kazumasa HONDA Ayako SUDO Kentaro IKEDA 《The Journal of reproduction and development》2013,59(6):569-574
The present experiments were undertaken to examine whether oxytocin cells in the
supraoptic nucleus receive synaptic inputs from the contralateral supraoptic nucleus
or paraventricular nucleus. Using urethane-anesthetized lactating rats, extracellular
action potentials were recorded from single oxytocin or vasopressin cells in the
supraoptic nucleus. Electrical stimulation was applied to the contralateral
supraoptic nucleus or paraventricular nucleus, and responses of oxytocin or
vasopressin cells were analyzed by peri-stimulus time histogram or by change in
firing rate of oxytocin or vasopressin cells. Electrical stimulation of the
contralateral supraoptic nucleus or paraventricular nucleus did not cause antidromic
excitation in oxytocin or vasopressin cells but caused orthodromic responses.
Although analysis by peri-stimulus time histogram showed that electrical stimulation
of the contralateral supraoptic nucleus or paraventricular nucleus caused orthodromic
excitation in both oxytocin and vasopressin cells, the proportion of excited oxytocin
cells was greater than that of vasopressin cells. Train stimulation applied to the
contralateral supraoptic nucleus or paraventricular nucleus at 10 Hz increased firing
rates of oxytocin cells and decreased those of vasopressin cells. The results of the
present experiments suggest that oxytocin cells in the supraoptic nucleus receive
mainly excitatory synaptic inputs from the contralateral supraoptic nucleus and
paraventricular nucleus. Receipt these synaptic inputs to oxytocin cells may
contribute to the synchronized activation of oxytocin cells during the milk ejection
reflex. 相似文献
23.
Hiroaki Kato Shuta Asai Ayako Yamamoto-Katou Hirofumi Yoshioka Noriyuki Doke Kazuhito Kawakita 《Journal of General Plant Pathology》2008,74(1):15-23
During defense responses, plant cells produce nitric oxide (NO), which may control many physiological processes. In a previous
study, we reported that nitrate reductase (NR) is responsible in part for INF1 elicitor-induced NO production in Nicotiana benthamiana, but the possibility remains that other NO-generating system(s) contribute to NO production. In mammalian cells, NO production
is catalyzed by NO synthase (NOS). However, NOS-like enzyme(s) have never been identified in plants, and only the gene for
Arabidopsis thaliana nitric oxide-associated 1 (AtNOA1) has been identified as a putative regulator of NOS activity in plants. In this study, we cloned NbNOA1, a homolog of AtNOA1, from N. benthamiana and investigated its involvement in NO production induced by INF1. The NbNOA1 gene was silenced by a virus-induced gene-silencing (VIGS) technique. NbNOA1-silenced plants had yellowish leaves. Silencing NbNOA1 partially decreased INF1-induced NO production, while overexpressing NbNOA1 did not affect NO production. Silencing NbNOA1 suppressed INF1-induced PR1a gene expression and increased susceptibility to Colletotrichum lagenarium. These results suggest that NbNOA1 is involved in INF1-mediated NO production and is required for defense responses.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB303300. 相似文献
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25.
Nomura H Ogiso M Yamashita M Takaku H Kimura A Chikasou M Nakamura Y Fujii S Watai M Yamada H 《Journal of agricultural and food chemistry》2011,59(9):5150-5158
Uptake and elimination of aflatoxins (AFs) by rainbow trout ( Oncorhynchus mykiss ) during a long-term (21 days) dietary exposure were studied to assess contamination by AFs in aquaculture fish fed AF-containing feed. The uptake factor (UF) of aflatoxin B(1) (AFB(1)) in muscle ranged from 0.40 × 10(-3) to 1.30 × 10(-3). AFB(1) concentrations in liver were 165-342 times higher than in muscle. AFs from feed were more highly accumulated in liver than in muscle. Aflatoxicol (AFL) and aflatoxin M(1) (AFM(1)) were detected in muscle and liver and also in the rearing water. AFL concentrations were higher than AFM(1) by 2 orders of magnitude in muscle, and AFL was a major metabolite of AFB(1). The elimination rate constants (α) of AFB(1) and AFL in muscle (1.83 and 2.02 day(-1), respectively) and liver (1.38 and 2.41 day(-1), respectively) were very large. The elimination half-life (t(1/2)) of AFB(1) was 0.38 days (9.12 h) in muscle and 0.50 days (12.00 h) in liver. The elimination half-life of AFL in muscle and liver was 0.34 day (8.16 h) and 0.29 day (6.96 h), respectively. These data show that AFs are eliminated rapidly and are not biomagnified in fish. Thus, AFB(1) concentration in muscle of fish fed AFB(1)-containing feed (ca. 500 μg/kg) decreased to below the detection limit (20 ng/kg) of the most sensitive analytical method at 1.54 days (36.96 h) after the change to uncontaminated feed. 相似文献
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27.
Akiho KATAYAMA Ayako MIYAZAKI Naohito OKAZAKI Teruko NAKAYAMA Osamu MIKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):931
A total of ten 1–2-year-old rabbits died within 2 weeks at a facility in Ehime prefecture in May 2019. Necropsy revealed liver discoloration and fragility, hemorrhage of some organs and blood coagulation failure. On histopathologic examination, necrotizing hepatitis was a common finding, together with fibrin thrombi in the small vessels and hemorrhage in some organs. Rabbit hemorrhagic disease (RHD) virus gene was detected in liver samples, and viral particles of approximately 32 nm in diameter were found in the cytoplasm of degenerated hepatocytes by electron microscopy. Phylogenetic analysis based on the partial VP60 gene sequence classified it as Lagovirus europaeus GI.2/RHDV2. This is the first confirmed outbreak of RHD caused by globally emerging GI.2/RHDV2 in Japan. 相似文献
28.
Mari ISHIDA Chiho KANEKO Takao IRIE Yoshino MARUYAMA Asami TOKUDA Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):454
Paragonimiasis is a zoonotic trematode infection caused by Paragonimus spp. To determine the recent status of Paragonimus infections in wild animals, this study investigated Paragonimus spp. in 39 raccoon dogs and 54 Japanese badgers from March 2019 to January 2021 in Miyazaki Prefecture, and examined metacercariae in freshwater crabs. Triploid P. westermani was found in one raccoon dog (2.6%), and metacercariae were recovered from Eriocheir japonica captured near the infected animal collected. One Japanese badger (1.9%) harbored P. skrjabini miyazakii; this prevalence was lower than the approximately 30% that was reported in the 1970s. Results indicated that zoonotic Paragonimus was sporadically prevalent in wild animals. Further investigation in various animals is awaited to elucidate current wildlife reservoirs for those Paragonimus. 相似文献
29.
Seiji TSUGE Mami NISHIDA Ayako FURUTANI Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(2):144-147
We isolated Xanthomonas oryzae pv. oryzae mutants deficient in the phosphoenolpyruvate : carbohydrate phos-photransferase system, a major glucose transport system in
bacteria, using the glucose analogue 3-deoxy-3-fluoro-d-glucose (3FG). Glucose uptake by the mutants was decreased to 15–35% of the parental strain, and growth greatly decreased
in synthetic media containing glucose as a sole sugar source. Growth of the mutants in rice leaves was, however, similar to
the wild type. These findings suggest that glucose is not necessarily a major carbohydrate source for X. o. pv. oryzae in rice leaves.
Received 11 August 2000/ Accepted in revised form 15 December 2000 相似文献
30.
Terasawa N Saotome A Tachimura Y Mochizuki A Ono H Takenaka M Murata M 《Journal of agricultural and food chemistry》2007,55(10):4154-4159
Zuiki, a stalk of taro (Colocasia esculenta), is a traditional vegetable in Japan. Raw zuiki is often boiled and vinegared to eat. The surface color of zuiki is reddish. Here, we isolated a red pigment from zuiki and identified it as cyanidin 3-rutinoside using instrumental analyses. The color of zuiki disappeared by boiling, but the zuiki turned red again in an acetic acid solution. It seems that the cyanidin 3-rutinoside that exists on the surface of zuiki elutes in boiling water and then, the pigment that seeps out from the inside of the zuiki is exposed to an acid solution, and its surface turns red again. The radical scavenging activity of purified zuiki anthocyanin was 114 mg equivalent to BHT/g. About half of the anthocyanin in fresh zuiki was washed out by boiling, and the radical scavenging activity of zuiki was definitely reduced. 相似文献