排序方式： 共有53条查询结果，搜索用时 78 毫秒
31.
Jahangiry Leila Khazaee-Pool Maryam Mahdavi Babak Ponnet Koen Sarbakhsh Parvin 《Tropical animal health and production》2019,51(2):419-428
Tropical Animal Health and Production - The purpose of this model-based study was to identify behavioral and environmental prevention factors for brucellosis and to determine the causal linkage... 相似文献
32.
33.
Babak Mamnoon Taghi Naserpour Farivar Ahmad Reza Kamyab Dariush Ilghari Ali Khamesipour Mohsen Karimi Arzenani 《Iranian Biomedical Journal》2016,20(1):56-62
Background:
Existence of bacterial host-cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor intensive and rather expensive. In this study, a rapid real-time PCR test was served as a method of choice to quantify the E. coli host- cell DNA contamination in widely used recombinant streptokinase (rSK), and alpha interferon (IFN-α) preparations.Methods:
A specific primer pair was designed to amplify a sequence inside the E. coli 16S rRNA gene. Serial dilutions of DNA extracted from E. coli host cells, along with DNA extracted from Active Pharmaceutical Ingredients of rSK, and IFN-α samples were subjected to an optimized real-time PCR assay based on SYBR Green chemistry.Results:
The test enabled us to detect a small quantity of genomic DNA contamination as low as 0.0002 pg in recombinant protein-based drugs. For the first time, this study showed that DNA contamination in rSK and IFN-α preparation manufactured in Pasteur Institute of Iran is much lower than the safety limit suggested by the US FDA.Conclusion:
Real-time PCR is a reliable test for rapid detection of host-cell DNA contamination, which is a major impurity of therapeutic recombinant proteins to keep manufacturers’ minds on refining drugs, and provides consumers with safer biopharmaceuticals. Key Words: DNA contamination, Real-time PCR, Streptokinase, Interferon-alpha (IFN-α) 相似文献34.
In this paper, the surface of magnetic manganese ferrite nanoparticles (MFN) was modified using cetyl trimethylammonium bromide (CTAB). The modified MFN was studied using Fourier transform infrared spectroscopy (FTIR). The adsorption capacity of surface modified MFN (MFN-CTAB) was investigated for dye removal for single and ternary systems. Three anionic dyes, C.I. Direct Red 80 (DR80), C.I. Direct Red 31 (DR31), and C.I. Acid Blue 92 (AB92), were used as model compounds. The effects of operational parameters on dye removal (i.e. adsorbent dosage, dye concentration and salt) and the kinetic and isotherm of dye adsorption were studied. The adsorption kinetic for the dyes was found to be well described by the pseudo-second order model. The maximum dye adsorption capacity (Q 0) of MFN-CTAB for DR80, DR31 and AB92 was 83 mg/g, 59 mg/g and 70 mg/g, respectively. The adsorption isotherm data were analyzed using the Langmuir, Freundlich, and Temkin equations. The results revealed that the Langmuir model fitted the adsorption data better. The results showed that the MFN-CTAB as a magnetic adsorbent might be a suitable alternative to remove dyes from colored aqueous solutions. 相似文献
35.
Somayeh Omidi Marziyeh Ebrahimi Hossein Janmohammadi Gholamali Moghaddam Zolfaghar Rajabi Babak Hosseintabar-Ghasemabad 《Journal of animal physiology and animal nutrition》2020,104(1):178-185
The present article was conducted to evaluate the effect of in ovo injection of arginine on hatchability, immune system and caecum microflora of broiler chickens. For this reason, 300 fertile eggs were used in a completely randomized design with three experimental treatments. The experimental groups included: 1%–0.5% l -arginine (100 eggs), 2%–1% l -arginine (100 eggs), 3- control [included both sham control (injection of distilled water; 50 eggs) and control (no injection; 50 eggs)], which were injected on d 14 of incubation. After hatching, chicks of each experimental group (0.5% l -arginine, 1% l -arginine, and control groups) were randomly divided into four equal groups (as replicates) and reared for 30 days. Weight and feeding of chickens were recorded. Next, blood samples of chickens were collected on day 30 to evaluate antibody titre. Also, chickens were slaughtered on 24 and 30 days of the experiment to evaluate immune system organs and caecum microflora. Based on the results, in ovo injection of l -arginine had no significant effect on hatchability, body weight, antibody titre, spleen, bursa of Fabricius and thymus weight (p > .05). On the other hand, treatments significantly affected feed intake and feed conversion ratio (p < .05). As a novel finding, in ovo injection of l -arginine increased caecal Lactobacillus (p < .01), while decreasing Coliform and Escherichia Coli bacteria (p < .01). However, treatments did not influence caecal Enterococcus (p > .05). The overall results indicated that in ovo injection of 0.5% l -arginine had a better improving effect on caecal microflora and then considered as a recommended level of the present experiment. 相似文献
36.
Beatty GL Chiorean EG Fishman MP Saboury B Teitelbaum UR Sun W Huhn RD Song W Li D Sharp LL Torigian DA O'Dwyer PJ Vonderheide RH 《Science (New York, N.Y.)》2011,331(6024):1612-1616
Immunosuppressive tumor microenvironments can restrain antitumor immunity, particularly in pancreatic ductal adenocarcinoma (PDA). Because CD40 activation can reverse immune suppression and drive antitumor T cell responses, we tested the combination of an agonist CD40 antibody with gemcitabine chemotherapy in a small cohort of patients with surgically incurable PDA and observed tumor regressions in some patients. We reproduced this treatment effect in a genetically engineered mouse model of PDA and found unexpectedly that tumor regression required macrophages but not T cells or gemcitabine. CD40-activated macrophages rapidly infiltrated tumors, became tumoricidal, and facilitated the depletion of tumor stroma. Thus, cancer immune surveillance does not necessarily depend on therapy-induced T cells; rather, our findings demonstrate a CD40-dependent mechanism for targeting tumor stroma in the treatment of cancer. 相似文献
37.
Babak Esmaeelian Catherine A. Abbott Richard K. Le Leu Kirsten Benkendorff 《Marine drugs》2014,12(1):17-35
Muricid molluscs are a natural source of brominated isatin with anticancer activity. The aim of this study was to examine the safety and efficacy of synthetic 6-bromoisatin for reducing the risk of early stage colorectal tumor formation. The purity of 6-bromoisatin was confirmed by 1H NMR spectroscopy, then tested for in vitro and in vivo anticancer activity. A mouse model for colorectal cancer was utilized whereby colonic apoptosis and cell proliferation was measured 6 h after azoxymethane treatment by hematoxylin and immunohistochemical staining. Liver enzymes and other biochemistry parameters were measured in plasma and haematological assessment of the blood was conducted to assess potential toxic side-effects. 6-Bromoisatin inhibited proliferation of HT29 cells at IC50 223 μM (0.05 mg/mL) and induced apoptosis without increasing caspase 3/7 activity. In vivo 6-bromoisatin (0.05 mg/g) was found to significantly enhance the apoptotic index (p ≤ 0.001) and reduced cell proliferation (p ≤ 0.01) in the distal colon. There were no significant effects on mouse body weight, liver enzymes, biochemical factors or blood cells. However, 6-bromoisatin caused a decrease in the plasma level of potassium, suggesting a diuretic effect. In conclusion this study supports 6-bromoisatin in Muricidae extracts as a promising lead for prevention of colorectal cancer. 相似文献
38.
Babak Baharvand Mansour Esmailidehaj Jamileh Alihosaini Shirin Bajoovand Saeedeh Esmailidehaj Zeynab Hafizibarjin 《Iranian Biomedical Journal》2016,20(1):41-48
Background:
This study was conducted to reveal that whether i.v. injection of oleuropein, the most potent polyphenolic antioxidant in olive leaf, has any effect on the magnitude of reperfusion arrhythmia in anesthetized rats or not.Methods:
Eighty male Wistar rats were divided into 8 groups of 10 each: groups 1 and 5 were assigned as the prophylactic and treatment control groups, groups 2 and 6 as the prophylactic and treatment groups with lidocaine (10 mg/kg), groups 3 and 4 as the prophylactic groups with 10 and 50 mg/kg oleuropein (i.v.), and groups 7 and 8 as the treatment groups with 10 and 50 mg/kg oleuropein (i.v.), respectively. Reperfusion injury was induced by 5-min regional ischemia and 15-min reperfusion of left anterior descending coronary artery. Heart rate, blood pressure, and electrocardiogram were monitored throughout the procedure.Results:
blood pressure was significantly decreased by infusion of 50 mg/kg oleuropein in groups 4 and 8, but unlike the lidocaine as a standard anti-arrhythmic drug in groups 2 and 5 had not significant effect on heart rate. The onset of arrhythmia in groups received oleuropein (groups 3, 4, 7, and 8) was significantly delayed. The mortality rate due to irreversible ventricular fibrillation was also significantly reduced in groups 3, 4, 7, and 8. The effect of lidocaine in groups 2 and 5 was more potent than that in oleuropein group.Conclusion:
These findings indicate that i.v. injection of oleuropein possibly through its antioxidant activity reduces the magnitude of reperfusion-induced arrhythmia.Key Words: Oleuropein, Arrhythmia, Reperfusion, Rats 相似文献39.
Babak Darvishi Kazem Pustini Ali Ahmadi Reza Tavakol Afshari Javad Shaterian Mohammad Hadi Jahanbakhshpour 《Journal of plant nutrition》2015,38(13):2096-2111
Under hydroponic condition, number and size of potato tubers are usually controlled by nutritional factors such as nitrogen (N), phosphorus (P), and pH. The main objective of the present study was to find an appropriate combination of N, P, and pH (with respect to tuber number) under hydroponic sand culture and to evaluate the physiological traits affected by nutrients and pH. A factorial experiment based on completely randomized design with four replications was conducted. Results showed that higher phosphorus concentration for 10 days increased tuber number per plant, but tuberization was not influenced by nitrogen interruption and intermittent reduction of pH. Neither N, P, nor pH treatments affected total nitrogen concentration of potato leaf, stem, and tuber. Higher phosphorus concentration increased the level of endogenous abscisic acid (ABA) and indole-3-acetic acid (IAA), induced tuberization and thereby increased net photosynthesis rate of potato plants. 相似文献
40.
Ahmadreza Abbasifar Fatemeh Shahrabadi Babak ValizadehKaji 《Journal of plant nutrition》2020,43(8):1104-1118
AbstractIn the present study, the green synthesis of Zn and Cu nanoparticles (NPs) was carried out via Zn and Cu ions reduction during their exposure to basil extract. The shape, size and chemical identity of the Zn and Cu NPs were determined using SEM and XRD analysis. To investigate the effects of the Zn and Cu NPs on the morphological and biochemical traits of basil plant, an experiment was conducted as a factorial based on completely randomized design with 4 levels of Zn NPs (0, 1000, 2000, and 4000?ppm) and 4 levels of Cu NPs (0, 1000, 2000, and 4000?ppm). Based on the results, nutrient treatments, especially 4000?ppm Zn NPs + 2000?ppm Cu NPs caused a significant increase in most morphological parameters. The application of the Zn and Cu NPs significantly affected concentrations of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid in the leaves of basil plants. The highest total phenolic and flavonoid content was obtained for 4000?ppm Zn NPs + 2000?ppm Cu NPs treatment. Plants treated with 4000?ppm Zn NPs + 0?ppm Cu NPs showed the highest antioxidant activity followed by 4000?ppm Zn NPs + 2000?ppm Cu NPs and 2000?ppm Zn NPs + 0?ppm Cu NPs. It can be concluded that foliar application of the Zn and Cu NPs is necessary for obtaining better quantity and quality in basil. 相似文献