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11.
Long term in vitro cultures of plant tissue spontaneously change at morphological, cellular and biochemical level. Horseradish tissues were cultivated in vitro for more than 16 years. Primary crown-gall tumours were induced with Agrobacterium tumefaciens on leaf explants excised from plantlets propagated in vitro. Subcultured on hormone-free MS medium, transformed tissue continued to grow in an unorganized way (TN tumour) or as teratogenous tumour (TM teratoma) forming malformed shoots which never rooted. The aim of this study was to establish the morphological and biochemical status of this long term cultures. The study was performed by verifying biochemical marker of transformation and morphogenesis, which had been investigated when the culture was established (peroxidase, PPX) as well as by introducing new markers which have not been analyzed so far in horseradish tissues (ascorbate peroxidase, APX; phenylalanine-ammonia lyase, PAL; and phenolics content). Morphological changes were observed only in teratoma tissue. Peroxidase activity and isoenzyme pattern indicated stability of horseradish tissue culture. The new biochemical markers were in a complete (PAL) and partial (APX) correlation with the PPX results and are therefore useful addition in biochemical characterisation of these tissues and can also be applied as markers of morphogenesis and tumorization. 相似文献
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Vujcić Z Milovanović A Bozić N Dojnov B Vujcić M Andjelković U Loncar N 《Journal of agricultural and food chemistry》2010,58(22):11896-11900
Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 °C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 °C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 °C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 °C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained. 相似文献
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Radmila Stikic Djordje Glamoclija Mirjana Demin Biljana Vucelic-Radovic Zorica Jovanovic Dusanka Milojkovic-Opsenica Sven-Erik Jacobsen Mirjana Milovanovic 《Journal of Cereal Science》2012
Quinoa is an Andean seed crop of many potential uses. In 2009 a field trial was carried out to explore the potential for quinoa growing in climatic conditions of South Eastern Europe. Even under rainfed conditions, without fertilization, a seed yield as high as 1.721 t ha−1 was obtained. Seed quality was remarkably good, with protein content ranging from 15.16 to 17.41 % on a dry weight basis, depending on whether seeds were processed. Amino acid and mineral composition revealed the potential of quinoa seeds as a valuable ingredient in the preparation of highly nutritious foods. Quinoa seeds had higher contents of most essential amino acids, especially lysine, than wheat flour. Dehulled quinoa seeds, devoid of saponins, were included into wheat bread formulations, with up to 20%, which resulted in a positive effect on the rheological characteristics of dough. Furthermore, protein content in bread was increased by around 2%. Sensory characteristics of breads were excellent also at the 20% supplementation level. The study of bread supplemented with quinoa seeds could enable the development of a range of new baking products with enhanced nutritional value. 相似文献
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Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis
Kovačević-Filipović M Ilić V Vujčić Z Dojnov B Stevanov-Pavlović M Mijačević Z Božić T 《Veterinary immunology and immunopathology》2012,145(1-2):120-128
Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content. 相似文献
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Sinisa Djurasevic Svetlana Bojic Biljana Nikolic Ivica Dimkic Zoran Todorovic Jelena Djordjevic Dragana Mitic-Culafic 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(4):295-301
The gut microbiota is composed of a diverse population of obligate and facultative anaerobic microorganisms which are shown to influence host metabolism and immune homeostasis. This study investigated the effects of virgin coconut oil on the weekly fasting glycaemia, daily food and water intake and weekly body mass gain over 16 weeks, as well as the changes in composition of gut microbiota in both non-diabetic and alloxan-induced diabetic rats. Although the intake of virgin coconut oil did not decrease the diabetes-induced hyperglycemia, it affected the secondary parameters, such as food and water intake and average body mass gain. Furthermore, its potential to positively affect the fecal microbiome was proved, since it significantly increased the abundance of probiotic bacteria, such as Lactobacillus, Allobaculum and Bifidobacterium species. 相似文献
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Emmy Van Balen 《European journal of plant pathology / European Foundation for Plant Pathology》1982,88(1):33-37
The application of electrical charges on carbon-coated formvar films had a marked effect on the trapping of virus particles by immunosorbent electron microscopical techniques. On grids, positively charged with ethidium bromide, numbers of virus particles were high, and almost equal to those trapped with the aid of protein A in combination with a negative charge.Samenvatting Het aanbrengen van elektrische lading op koolstof-formvar vliezen beïnloedde de adsorptie van virusdeeltjes aan het vlies, bedekt met homoloog antiserum (-globulinen). Op vliezen met een positieve lading werd een veel groter aantal virusdeeltjes waargenomen dan op de neutrale of negatief geladen vliezen. Positief geladen vliezen werden verkregen door behandeling met ethidium-bromide. Negatief geladen vliezen werden verkregen door behandeling met geïoniseerd argongas. Op de negatief geladen vliezen bleken grote hoeveelheden verontreinigingen van allerlei aard te precipiteren. Bovendien was het beeld van de virusdeeltjes vaag. Negatief geladen vliezen gaven wel goede resultaten ten aanzien van het vangen van virusdeeltjes in combinatie met proteine A en -globulinen. 相似文献
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Vesna N. Despotovi? Biljana F. Abramovi? Daniela V. ?oji? Sanja J. Kler Milena B. Dalmacija Luka J. Bjelica Dejan Z. Or?i? 《Water, air, and soil pollution》2012,223(6):3009-3020
The efficiency of the photocatalytic degradation of the herbicide quinmerac in aqueous TiO2 suspensions was examined as a function of the type of light source, TiO2 loading, pH, temperature, electron acceptors, and hydroxyl radical (?OH) scavenger. The optimum loading of catalyst was found to be 0.25?mg?mL?1 under UV light at pH?7.2, with the apparent activation energy of the reaction being 13.7?kJ?mol?1. In the first stage of the reaction, the photocatalytic degradation of quinmerac (50???M) followed approximately a pseudo-first order kinetics. The most efficient electron acceptor appeared to be H2O2 along with molecular oxygen. By studying the effect of ethanol as an ?OH scavenger, it was shown that the heterogeneous catalysis takes place mainly via ?OH. The results also showed that the disappearance of quinmerac led to the formation of a number of organic intermediates and ionic byproducts, whereas its complete mineralization occurred in about 120?min. The reaction intermediates (7-chloro-3-methylquinoline-5,8-dione, three isomeric phenols hydroxy-7-chloro-3-methylquinoline-8-carboxylic acids, and 7-chloro-3-(hydroxymethyl)quinoline-8-carboxylic acid) were identified and the kinetics of their appearance/disappearance was followed by LC?CESI?CMS/MS. Tentative photodegradation pathways were proposed and discussed. The study also encompassed the effect of quality of natural water on the rate of removal of quinmerac. 相似文献
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Svetlana Milija?evi?-Mar?i? Karl-Heinz Gartemann Jonas Frohwitter Rudolf Eichenlaub Biljana Todorovi? Emil Rekanovi? Ivana Poto?nik 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(4):697-711
Sixty-eight Clavibacter michiganensis subsp. michiganensis (Cmm) strains from recent outbreaks of bacterial wilt and canker in Serbia were collected from several tomato growing regions during a three-year period. The pathogen was identified based on bacteriological characteristics and pathogenicity tests and the identity of strains was confirmed by DAS ELISA and PCR amplification using primers CMM5/6 and PSA4/R. The strains showed homogeneity in biochemical and physiological properties. However, pathogenicity tests revealed differences in virulence that are presumably due to a loss of the pat-1 gene. Further strain characterization using DNA-based methods revealed a high diversity of the Serbian Cmm strains. Based on multi-locus sequence typing (MLST) analyses of five genes, Cmm strains were divided into seven groups. The pulsed-field gel electrophoresis (PFGE) pattern of a selection of strains supported the groupings based on trees of the kdpA/sdhA sequences. On the other hand, groupings made according to PFGE and MLST were not correlated to plasmid content in all cases. This study suggested that high genetic variability of the Serbian Cmm strains was detected both in MLST and PFGE analyses, and could have resulted either from new Cmm strains being introduced by seeds from different origins or as a consequence of an intraspecific hybridization process. In addition, this study proposed MLST as an efficient tool in epidemiological studies, population biology investigations and tracking the routes of transmission of pathogens. Four of the five house-keeping genes (kdpA, sdhA, ligA and gyrB) selected to characterize Cmm strains proved to be suitable for the MLST analysis. This is the first study carried out on the characterization of Cmm using MLST. 相似文献