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Mimica-Dukic N Bozin B Sokovic M Simin N 《Journal of agricultural and food chemistry》2004,52(9):2485-2489
The present study describes antimicrobial and free radical scavenging capacity (RSC) together with the effects on lipid peroxidation (LP) of Melissa officinalis essential oil. The chemical profile of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). RSC was assessed measuring the scavenging activity of essential oil on the 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and OH(*) radicals. The effect on LP was evaluated following the activities on Fe(2+)/ascorbate and Fe(2+)/H(2)O(2) systems of induction. The antimicrobial activity was tested against 13 bacterial strains and six fungi. The examined essential oil exhibited very strong RSC, reducing the DPPH radical formation (IC(50) = 7.58 microg/mL) and OH radical generation (IC(50) = 1.74 microg/mL) in a dose-dependent manner. According to the GC-MS and TLC (dot-blot techniques), the most powerful scavenging compounds were monoterpene aldehydes and ketones (neral/geranial, citronellal, isomenthone, and menthone) and mono- and sesquiterpene hydrocarbons (E-caryophyllene). Very strong inhibition of LP, particularly in the Fe(2+)/H(2)O(2) system of induction (94.59% for 2.13 microg/mL), was observed in both cases, also in a dose-dependent manner. The most effective antibacterial activity was expressed on a multiresistant strain of Shigella sonei. A significant rate of antifungal activity was exhibited on Trichophyton species. 相似文献
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Epithelial cells respond to physicochemical damage with up-regulation of major histocompatibility complex-like ligands that can activate the cytolytic potential of neighboring intraepithelial T cells by binding the activating receptor, NKG2D. The systemic implications of this lymphoid stress-surveillance response, however, are unknown. We found that antigens encountered at the same time as cutaneous epithelial stress induced strong primary and secondary systemic, T helper 2 (T(H)2)-associated atopic responses in mice. These responses required NKG2D-dependent communication between dysregulated epithelial cells and tissue-associated lymphoid cells. These data are germane to uncertainty over the afferent induction of T(H)2 responses and provide a molecular framework for considering atopy as an important component of the response to tissue damage and carcinogenesis. 相似文献
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Stanojevic SP Barac MB Pesic MB Vucelic-Radovic BV 《Journal of agricultural and food chemistry》2011,59(13):7368-7376
Protein quality in six soybean varieties, based on subunit composition of their protein, was correlated with quality of the produced tofu. Also, protein changes due to a pilot plant processing method involving high temperature/pressure and commercial rennet as coagulant were assessed. In each soybean variety, glycinin (11S) and β-conglycinin (7S) as well as 11S/7S ratio significantly changed from beans to tofu. Between varieties, the 11S/7S protein ratio in seed indicated genotypic influence on tofu yield and gel hardness (r = 0.91 and r = 0.99, respectively; p < 0.05). Also, the 11S/7S ratio correlated with soymilk pH (r = 0.89, p < 0.05), leading to a relationship between soymilk pH with protein recovery and yield of tofu (r = 0.94 and r = 0.91, respectively; p < 0.05). The soybean β'-subunit of 7S protein negatively influenced tofu hardness (r = -0.91, p < 0.05). Seed protein composition and proportion of 7S protein subunits under the applied production method had an important role in defining tofu quality. 相似文献
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Ivana Potočnik Jelena Vukojević Mirjana Stajić Emil Rekanović Miloš Stepanović Svetlana Milijašević Biljana Todorović 《Crop Protection》2010
Twenty Cladobotryum dendroides isolates from Serbian Agaricus bisporus farms collected during 2003–2007, and the F56 strain of A. bisporus were tested in vitro for sensitivity to tea tree oil (Timorex 66 EC), a biofungicide, in comparison with prochloraz–manganese (Octave WP). The efficacies of tea tree oil and prochloraz–manganese were evaluated in a mushroom growing room, after application at standard product application rates and a combination of the two at respective proportion of 20:80%. Tea tree oil was considerably less toxic than prochloraz–manganese in vitro to C. dendroides isolates (ED50 112.9–335.8 mg l−1) and A. bisporus F56 strain (98.0 mg l−1), although neither fungicide was lethal to the pathogen. The biological efficiency of tea tree oil was higher than in treatments with the reference formulation of prochloraz–manganese. In our in vivo trials, no negative interference of the biofungicide with A. bisporus physiology was observed. Tea tree oil applied at the standard product application rate caused a significant reduction in cobweb disease levels in the A. bisporus growing room. Timorex 66 EC should be tested further in combination with other biofungicides to investigate the effectiveness of various mixtures for A. bisporus disease control. 相似文献
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Viša Tasić Renata Kovačević Biljana Maluckov Tatjana Apostolovski – Trujić Branislava Matić Mira Cocić Mirjana Šteharnik 《Water, air, and soil pollution》2017,228(6):230
The content of As and heavy metals (Pb, Cd, Ni, and Cu) in total suspended particulate (TSP) and PM10 at 3 locations (Park, Institute, and Jugopetrol) near the copper smelter in Bor (Serbia) has been analyzed within the period 2004 to 2015 with the aim of investigating the seasonal and spatial changes of those pollutants in the suspended particles. The content of As in TSP and PM10 was over the annual EU limit value at all measuring points during the entire period of observation, while contents of Cd and Pb were periodically above the annual EU limits. There were no statistically significant seasonal changes between mean levels of the observed elements in the cold (October–March) and warm (April–September) periods during the year. A strong and moderate positive correlation was detected between the concentrations of each particular element (except Ni) at all measuring points. Additionally, Cd was the most enriched element followed by Pb, As, and Cu, while Ni was low-enriched. The constant air pollution with As particles, sometimes in concentrations even 20 times higher than the permitted annual value, requires urgent undertaking of concrete actions in order to reduce anthropogenic emission of suspended particles in Bor. 相似文献
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Marija Vukcevic Biljana Pejic Mila Lausevic Ivana Pajic-Lijakovic Mirjana Kostic 《Fibers and Polymers》2014,15(4):687-697
Short hemp fibers, acquired as a waste from textile industry, were used as an efficient biosorbent for removal of zinc ions from polluted water. In order to obtain the material with better sorption properties, short hemp fibers were subjected to oxidative and alkali treatment. The following factors that may influence the sorption properties of short hemp fibers were examined: fiber structure and morphology were characterized by iodine sorption, water retention and scanning electron microscopy, while specific surface area was determined by BET method. Additionally, the amount of carboxyl groups was determined by calcium-acetate method, and the point of zero charge of the short hemp fibers samples was determined by the solid addition method. Biosorption of zinc ions was evaluated through the total uptake capacity, equilibrium and kinetic data. Obtained data were analyzed by nonlinear Langmuir and Freundlich isotherms, as well as pseudo-first and pseudo-second order kinetic models, and the best fitting model was chosen using Akaike information criterion. Chemical modification, used in this work, leads to structural and morphological changes of short hemp fibers, and improvement of their sorption properties. It was found that sorption properties of short hemp fibers are predominantly influenced by surface acidity and the amount of functional groups, while fiber structure and specific surface area have a secondary role in the biosorption of zinc ions. Akakike information criterion values showed that biosorption of zinc ions on all tested hemp fiber samples obey the pseudo-second order adsorption kinetics, while experimental isotherm data fit better with Langmuir model. Biosorption of zinc ions on the hemp fibers is a predominantly chemical process, which mainly follows the mechanism of ion exchange on acidic functional groups, and occurs through the fast surface adsorption, intraparticle diffusion and final equilibrium stage. 相似文献
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Sladjana Savić Radmila Stikić Biljana Vucelić Radović Biljana Bogičević Zorica Jovanović Vesna Hadži-Tašković Šukalović 《Scientia Horticulturae》2008
The effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on tomato fruit growth and cell wall peroxidase activity in tomato exocarp were investigated in growth chamber conditions. The RDI treatment was 50% of water given to fully irrigated (FI) plants and the PRD treatment was 50% of water of FI plants applied to one half of the root system while the other half dried down, with irrigation shifted when soil water content of the dry side decreased 15–20%. RDI significantly reduced fruit diameter, though PRD reduced fresh weight while having no significant effect on fruit diameter. The activity of peroxidase was significantly higher in RDI and PRD treated plants compared to those of FI. Differences between RDI and PRD were expressed on temporal basis. In the fruits of RDI treated plants peroxidase activity began to increase in the phase when fruit growth started to decline with the peak of enzyme activity of 6.1 HRPEU g−1 FW reached in the phase of mature green fruits when fruit growth rate was minimal. Increase of peroxidase activity in PRD fruits coincided with the ripening phase and the peak of enzyme activity (5.3 HRPEU g−1 FW) was measured at the end of fruit ripening. These data potentially identified contrasting and different roles of tomato exocarp cell wall peroxidase in RDI and PRD treated plants. In RDI treated plants peroxidase may have a role in restricting fruit growth rate, although the increase in enzyme activity during ripening of PRD treated fruit pointed out that peroxidase may also control fruit maturation by inducing more rapid process. 相似文献
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Djina Božović Biljana Lazović Sezai Ercisli Mirjana Adakalić Vučeta Jaćimović Isik Sezer Aysen Koc 《Erwerbs-Obstbau》2016,58(2):93-102
Autochthonous apple varieties are still keeping their importance in Montenegro and they are valuable resources as human food and an important part of rural landscape. The aim of this study was to study and preserve morphological diversity of 30 autochthonous apple varieties in Montenegro between 2008 and 2010. We found a great variation on blooming period and maturation time among varieties and classified them as very early, early, mid and late for blooming and early, middle, late and very late for maturation. Fruit weight varied in wide range from 40.76 g to 206.74 g and ‘Krupnaja’, ‘Krstova?a’ and ‘Babova?a’ produced biggest fruits (191.83–206.74 g). Soluble solid content (SSC) varied between 11.0 to 16.1?% among varieties and ‘Borova?a’, ‘Aleksandrija’, ‘Krstova?a’, ‘Dapsi?anka’, ‘Bosnika’, ‘Rebra?a’ and ‘Babova?a’ had the highest SSC values imply that they can be commercially used in production of spirits, wine, concentrate and jam and also for drying. Based on 3-year average data for 25 properties, UPGMA (Unweighted Pair Group Method with Arithmetic Mean) dendrogram showed a high degree of variability among the studied varieties dividing them into 5 groups and 3 independent accessions. 相似文献