Associations of leptin gene polymorphisms with production traits in pigs   总被引：1，自引：0，他引：1  

J. de Oliveira Peixoto  S.E. Facioni Guimarães  P. Sávio Lopes  M.A. Menck Soares  A. Vieira Pires  M.V. Gualberto Barbosa  R. de Almeida Torres  & M. de Almeida e Silva 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(6):378-383

The associations of leptin (LEP) gene polymorphisms C798T, T2411C, T3266G and T3469C with production traits were investigated in a F2 pig population produced by divergent crosses. The statistical model included genotype, sex, batch and genotype by sex interaction as fixed effects and sire as random effect. Polymorphism C798T was associated with variation in total teat number (p < 0.02) and left teat number (p < 0.03), and polymorphism T3469C was associated with weight at 21 days (p < 0.03), 42 days (p < 0.05), 63 days (p < 0.02) and 77 days of age (p < 0.04) as well as feed intake (p < 0.01), average daily gain (p < 0.01), feed conversion (p < 0.01), bacon depth (p < 0.03) and slaughter weight (p < 0.03). Phenotypic associations were also performed by combining T3469C and C798T genotypes. Interaction between C798T genotypes and sex was observed for some traits. LEP genotypes had significant influence on performance traits, and can be considered as potential genetic markers for selection. However, these results have to be validated in commercial herds.  相似文献   

Modulatory effects of chitosan adipate on the T and B lymphocyte subsets in mice     

Bo?ena Obmińska-Mrukowicz  Marianna Szczypka  Bartosz Gaw?da 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(2):157-160

This study examined the subsets of T lymphocytes in the thymus, spleen and mesenteric lymph nodes as well as the subsets of B lymphocytes in the spleen and mesenteric lymph nodes in mice administered chitosan adipate (20 mg/kg) intraperitoneally once or four times at 24 h intervals. The results showed that chitosan adipate decreased the percentage of immature CD4⁺CD8⁺ thymic T cells and increased the percentage of mature CD4⁺ and CD8⁺ thymocytes. The most significant stimulating effect was observed after four injections. A single exposure to chitosan adipate increased the percentage of CD4⁺ mesenteric lymph node cells, but four injections of the drug increased the percentage of CD4⁺ and CD8⁺ mesenteric lymph node cells. Chitosan adipate had no effect on the subset of splenic T cells. In contrast, chitosan adipate administered either once or four times increased the percentage of CD19⁺ splenocytes but had no effect on the percentage of CD19⁺ mesenteric lymph node cells. Overall, chitosan adipate induces the maturation and differentiation of thymocytes, and regulates the number of B splenic cells and lymph node T cells irrespective of the number of doses.  相似文献   

Assessment of Sperm Apoptosis in Cryopreserved Bull Semen After Swim-up Treatment: A Flow Cytometric Study     

A Chaveiro  P. Santos  FM da Silva 《Reproduction in domestic animals》2007,42(1):17-21

The techniques used to prepare bovine spermatozoa for in vitro fertilization, to enhance the percentage of motile sperm cells include the swim-up (SU) method, among others. The objective of the present study was to evaluate the phosphatidylserine (PS) translocation and plasma membrane integrity as the indicator of apoptosis and necrosis in post-thaw bull sperm after SU treatment using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) assay. A flow cytometric method was employed to measure apoptosis levels on frozen-thawed bull spermatozoa. The assay detects PS translocation across the plasma membrane using a fluorescein-labelled annexin-V and PI. By using the annexin V/PI assay four different subpopulations of sperm were observed: (i) a population of apoptotic sperm, labelled with annexin V-FITC but not with PI; (ii) a population of early necrotic spermatozoa, sperm labelled with annexin-FITC and PI; (iii) a population of necrotic sperm, labelled with PI but not with annexin-FITC; and (iv) a population of fully viable sperm cells, sperm not labelled with annexin V-FITC and without PI. Results clearly indicated that SU technique itself could have an adverse effect on the spermatozoa membrane stability. It has also been found, significant differences between bulls in the levels of apoptotic sperm, after SU treatment.  相似文献   

Prevalence and genetic characterization of Hepatitis E virus in paired samples of feces and serum from naturally infected pigs          下载免费PDF全文

Salceda Fernndez-Barredo  Carolina Galiana  Angel García  Maria Teresa Gmez-Muoz  Santiago Vega  Manuel A. Rodríguez-Iglesias    Maria Teresa Prez-Gracia 《Canadian journal of veterinary research》2007,71(3):236-240

This study describes the distribution of Hepatitis E virus (HEV) in a naturally infected swine population and the genetic relatedness of HEV strains on swine farms in Spain. Of fecal and serum samples collected from 131 pigs and manure-ditch samples collected from 17 farms, HEV was detected in 16%, 14%, and 59%, respectively, for an overall prevalence rate of 23%. The maximum prevalence rates for feces and serum were in pigs 5 to 12 wk old. A high prevalence of the virus in feces (18%) was observed in sows. Gene sequencing was performed on 6 strains from feces, serum, and manure ditch: the nucleotide identities varied from 81.5% to 99% when compared with those of other strains of genotype 3 isolated from swine. This is the first study in Europe to show the variation in virus distribution by age in feces and serum in a naturally infected swine population.  相似文献   

Effect of dietary protein deficiency on the performance of turkeys and chickens     

H E Ekperigin  T Silva  R H McCapes 《Avian diseases》1983,27(1):304-307

Two field occurrences of protein-deficiency disease were reported in turkeys and chickens. The disease caused unthriftiness, poor growth, and the exhibition of pica in flocks of 15-week-old pedigree large white turkeys, and it resulted in a sharp drop (18%) in egg production in a flock of 60-week-old white leghorn layers. In each case, the disease was cured by replacing the protein-deficient diet with a protein-sufficient one.  相似文献   

Research note: effect of increasing dietary concentrate levels on microbial biotin metabolism in the artificial rumen simulation system (RUSITEC).     

H Abel  I Immig  C da C Gomez  W Steinberg 《Archives of Animal Nutrition》2001,55(4):371-376

The effect of varying hay/barley-proportions in the feed ration on biotin metabolism of rumen microbes was studied by means of the rumen simulation technique RUSITEC. The stepwise replacement of hay by barley decreased dietary biotin and the net output of biotin by the microbial metabolism. It is concluded that rumen microbes utilise more and/or synthesize less biotin with increasing proportions of dietary barley. These results indicate that a critical reconsideration of current views with regard to the supply and requirement of the high yielding dairy cow for biotin is necessary.  相似文献   

The Supracloacal Chromolipoid Body of the Black Vulture (Coragyps atratus): Anatomical, Histological and Histochemical Considerations          下载免费PDF全文

W. M. Corrêa  G. H. Silva  L. T. Kawabe    A. C. Carneiro 《Canadian journal of veterinary research》1969,33(2):160-163

During histological and physiological investigations of black vultures (Coragyps atratus) dissection revealed the presence of an “organ”, the supracloacal chromolipoid body, which has no counterpart among other warm blooded animals.

The organ occurs in both sexes and in birds of different ages. It is located in the median sagittal plane dorsal to the cloaca. It is enveloped by a smooth muscle-connective tissue capsule and has a rich blood supply.

The supracloacal body is yellow-brown in color being composed chiefly of islands of pigment cells.

Histochemically, the pigment is a chromolipoid as defined by Ciaccio.

  相似文献   

The detection of Toxoplasma gondii by comparing cytology, histopathology, bioassay in mice, and the polymerase chain reaction (PCR)     

da Silva AV  Langoni H 《Veterinary parasitology》2001,97(3):191-198

The objective of this study was to compare the different methods of detecting Toxoplasma gondii in sheep tissue, tested serologically positive by the indirect immunofluorescent antibody test (IFAT). Brain, diaphragm, and blood samples were collected from 522 sheep slaughtered at the S?o Manuel abattoir, S?o Paulo State, Brazil. Brain and diaphragm samples from IFAT seropositive animals were digested by both trypsin and pepsin and then injected into mice. Part of the digested samples was used to prepare slides for Giemsa staining and in the polymerase chain reaction (PCR). Tissue fragments were fixed in formalin and examined using hematoxilin-eosin (HE). Forty of the sheep (7.7%) were IFAT positive. T. gondii was isolated in 23 (59.0%) of the 39 mice with pepsin-digested brain samples and in 27 (69.0%) of the 39 with trypsin-digested brain samples. Injection of diaphragm samples led to T. gondii isolation in 26 (66.7%) of the 39 pepsin-digested samples and 21 (53.8%) of the 39 trypsin-digested samples. Cytological and hystopathological examination of both brains and diaphragms was negative in all examined sheep. PCR was positive in 7 (17.9%) of the trypsin and 2 (5.1%) of the pepsin-digested samples, while 9 (23.1%) of the trypsin and 3 (7.7%) of the pepsin-digested samples showed T. gondii DNA. T. gondii isolation rate in mice (n = 34; 85.0%) was significantly higher than detection by PCR (n = 15; 37.5%).  相似文献   

Occurrence of Anti-Toxoplasma gondii Antibodies in Dogs in the Urban Area of Monte Negro,Rondônia,Brazil     

Cañón-Franco WA  Bergamaschi DP  Labruna MB  Camargo LM  Silva JC  Pinter A  Gennari SM 《Veterinary research communications》2004,28(2):113-118

The prevalence of anti-Toxoplasma gondii antibodies in dogs in an urban area of the municipality of Monte Negro, Rondônia, Brazil, was evaluated using an indirect immunofluorescent antibody test (IFAT). Blood samples were taken from 157 dogs living in 85 of the 94 blocks of the city. A seropositivity of 76.4% (120/157) was found and associations between the prevalence and the variables sex, age, type of raising and food were studied. The prevalence tended to increase with age (p<0.05); dogs over 24 months old had 85.5% (100/117) positivity, compared with 50% (20/40) in dogs less than 24 months old, showing postnatal exposure to the agent. It was also observed that dogs with access to the streets showed greater prevalence (84.9%) than companion animals (58.8%). There was no association between sex or the type of food (home-made or commercial) and anti-T. gondii antibodies.  相似文献   

Diagnostic potential of natriuretic peptides in the occult phase of golden retriever muscular dystrophy cardiomyopathy     

Chetboul V  Tessier-Vetzel D  Escriou C  Tissier R  Carlos C  Boussouf M  Pouchelon JL  Blot S  Derumeaux G 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):845-850

The objective of the study was to determine whether the plasma concentrations of atrial and brain natriuretic peptides (ANP and BNP, respectively) could be reliable markers of cardiac alterations during occult cardiomyopathy in Golden Retriever Muscular Dystrophy (GRMD). Fifty Golden Retrievers without any clinical or radiographic sign of heart disease were included in this study (21 GRMD dogs and 29 controls). Controls and GRMD dogs were divided into 2 subgroups according to age (< and > or =12 months old, respectively). All dogs underwent echocardiography and determination of BNP and ANP plasma concentrations by radioimmunoassay. No ventricular dilatation or dysfunction was observed in either control or GRMD dogs. ANP plasma concentration did not differ significantly between controls and GRMD dogs (mean +/- SD = 72 +/- 49 versus 58 +/- 23 pg/mL, respectively, P = .21). This finding was confirmed in both subgroups of dogs (ie, those < and > or =12 months old). In contrast, BNP plasma concentrations were significantly higher in GRMD dogs than in controls (mean +/- SD = 117 +/- 92 versus 46 +/- 22 pg/mL, respectively, P < .05). In dogs > or =12 months old, sensitivity and specificity of BNP for identifying GRMD with a cutoff of 65 pg/mL were 78 and 86%, respectively. For the same cutoff value, sensitivity dropped to 42%, whereas specificity reached 100% in dogs <12 months old. In conclusion, BNP may be a useful biochemical marker of asymptomatic cardiomyopathy. However, this peptide does not allow very early detection because its optimal discriminatory power was observed in adult dogs (ie, > or =12 months of age).  相似文献