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171.
A capillary sieving electrophoretic method for protein analysis and molecular weight determination was used to characterize ciders from Asturias, northern Spain. The total protein content (Bradford method) and the foam parameters (Bikerman method) were also analyzed to complete this characterization. The polypeptide profile, based on the molecular weight, together with exploratory and classification techniques, that is, principal component analysis (PCA) or linear discriminant analysis (LDA), allowed ciders to be differentiated on the basis of their foam assessment and the apple juice extraction technology used in the cidermaking process. In addition, the application of correlation analysis, that is, canonical correlations (CCA) or partial least-squares (PLS), revealed that the proteins with higher molecular weight were more relevant with respect to cider foamability. PLS analysis also provided a mathematical equation that is able to predict the stabilization time of foam from the polypeptide profile of the cider, because this is the foam parameter most influenced by these compounds.  相似文献   
172.
We combined size-at-age and underwater survey (UVS) data to investigate the spatio-temporal distribution of life-history stages of the bristletooth surgeonfish, Ctenochaetus striatus, around Tutuila Island, American Samoa. Three age-based size categories (recruits, juveniles and adults) were determined by examining the relationship between size, age and gonad maturity. The results from combining size-at-age with UVS density data showed varying densities at the scales examined (years, exposure, sector, and habitat) on the fore-reef slopes of Tutuila Is. While recruit densities were uniform, juvenile densities varied by year, exposure and sector, and adult densities were significantly higher at topographic sites that were comparatively more exposed to water motion than inside bays. Densities of recruits were positively correlated with habitat (rubble) illustrating the importance of this substratum for the survivorship of early life history stages. Densities of adults were positively correlated with coralline algae, which may suggest co-occurrence responding to similar physical environment of adult habitat that is possibly indirectly correlated to feeding. However, densities of juveniles were negatively correlated with food source although the variance accounted for was low. We hypothesize that some other substratum may possibly be important for juveniles. There was a negative correlation between recruit and adult densities, indicating the presence of ontogenetic shifts in habitat with age and size. It is proposed that ecosystem-based fishery management of C. striatus in Tutuila Is should consider the high abundance of adults in exposed topographic habitats in the establishment of marine protected areas. We have shown that size-at-age data when combined with underwater fish surveys significantly expands its utility in the study of the population structure of a coral reef fish thus allowing the identification of areas characterized by high abundance of particular life stages for management.  相似文献   
173.
Recent advances on feeding in intensive rearing of juvenile astacids enable research of other factors influencing growth and survival without the confounding effect of inadequate feeding. Two experiments were conducted to evaluate the effects of the number of shelters per crayfish and to test different lighting conditions. Stage 2 juvenile Pacifastacus leniusculus were stocked in fibreglass tanks and fed a dry diet for salmonids combined with restricted amounts of decapsulated Artemia cysts. In experiment 1, four, two or one shelters per crayfish were tested for 80 days. No significant differences were found among groups either in survival (final mean: 86.67%) or in growth [final mean: 11.41 mm mean carapace length (CL), 355.45 mg mean weight]. In experiment 2, three lighting conditions were tested for 120 days: continuous lighting of 925 lx, continuous darkness and natural photoperiod. Survival rates ranged from 76.7% to 88.3%, with no significant differences among groups. The crayfish kept under continuous darkness grew faster (final mean: 12.70 mm CL, 543.08 mg weight) than those reared under the other two light conditions. This study shows that, under improved feeding conditions, a minimum number of shelters can be provided, and faster growth can be obtained in continuous darkness.  相似文献   
174.
The reproduction of Holothuria floridana in Campeche bay, southern Gulf of Mexico, was investigated over a period of 12 months by macroscopic and histological examination of the gonad and gametogenesis. A total of 1938 specimens were collected. Their body gutted weight and body gutted length ranged from 15 to 225 g and 5 to 21 cm, respectively. Percentage of the gonad weight to the gutted body weight (gonad index, GI) increased steadily from December to April, rapidly declined in May, and remained low during the following months. GI displayed a negative correlation with temperature of the habitat and no correlation with salinity. The gonad maturity scale with five stages was developed from macroscopic and microscopic observations; I, recovery; II, growing; III, early mature; IV, mature and V, spent. Stages III to V were observed all around the year, suggesting that this species displays continuous reproduction with two peaks of spawning events; from March to April and in September. The presence of viscera regurgitated individuals caused high variability in GI. The sex-ratio varied from 1:1 (December 2013 to June 2014), to 1.37:1 (November 2013); we suggest that this may be the consequence of unintentional selective fishing. The body length and body weight at the first maturity were calculated to be 13.4 cm and 87.0 g, respectively. For resource management it will be necessary to address some reference points like harvest season and avoidance of catches shorter than length at first maturity.  相似文献   
175.
The use of trunk diameter fluctuations and their derived parameters for irrigation scheduling in woody crops is reviewed. The strengths and weaknesses of these continuously measured plant-based water stress indicators compared with other discretely measured indicators for diagnosing plant water status in young and mature trees are discussed. Aspects such as sensor reading variability, signal intensity and the relationship between trunk diameter fluctuations and plant water status are analyzed in order to assess their usefulness as water stress indicators. The physiological significance of maximum and minimum daily trunk diameter and maximum daily trunk shrinkage (MDS) are also considered. Current knowledge of irrigation protocols and baselines for obtaining maximum daily trunk shrinkage reference values is discussed and new research objectives are proposed. We analyze the response of woody crops to continuous deficit irrigation scheduled by maintaining MDS signal intensity at threshold values to generate mild, moderate and severe water stress and assess the possibility of using linear variable displacement transducer (LVDT) sensors in trunk as a precision tool for regulated deficit irrigation scheduling. Finally, the possibility of using MDS signal intensity as a tool to match the irrigation regime to tree water requirements is also reviewed.  相似文献   
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