Molecular sequence data of Thecaphora frezii affecting peanut crops in Argentina     

Cinthia Conforto  Ignacio Cazón  Franco D. Fernández  Adriana Marinelli  Claudio Oddino  Alejandro M. Rago 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(4):663-666

In Argentina, Thecaphora frezii is the causal agent of peanut smut, causing severe yield losses in peanut growing areas. In this work sequence data of the large ribosomal subunit (28S rDNA) of four T. frezii specimens from different production regions are provided. A fragment of about 650 bp was amplified by PCR from each of the isolates analyzed. The analysis of the sequences demonstrated 100 % identity among the four specimens and 92–96.3 % identity with sequences of other species of Thecaphora. This is the first study on molecular sequences of this pathogen.  相似文献   

The effect of ethephon on the growth and development of Liatris spicata     

S. BañóN  A. González  J. A. Fernández  J. A. Franco 《The Journal of Horticultural Science and Biotechnology》2013,88(6):851-855

Summary

The first experiment studied the effect of applying ethylene biosynthesis regulating compounds (ethephon 1000 ppm, silver thiosulphate 0.4 mM and cobalt chloride 5 mM) on the growth and development of Liatris spicata cv. Callilepis. The results pointed to the important growth retardant properties of ethephon and the increased number of flowering stems per corm which could be obtained by pre-planting application. A second experiment aimed to optimize ethephon treatment in Liatris by applying the growth retardant in different concentrations (100, 700, 1300, 1900 and 2500 ppm) at three stages of development: stage I (pre-planting), stage II (immediately after the formation of basal leaf rosette) and stage III (when the main shoot began its rapid longitudinal growth). Besides confirming the effects produced by ethephon in the first experiment, the results suggested that the application of high ethephon concentrations (1900 and 2500 ppm) during stage II of growth produced many miniature flowering stems coming from the principal or secondary shoot.  相似文献   

Effect of two irrigation rates on yield,incidence of blossom-end rot,mineral content and free amino acid levels in tomato cultivated under drip irrigation using saline water     

J. A. Franco  P. J. Pérez-Saura  J. A. Ferná Ndez  M. Parra  A. L. García 《The Journal of Horticultural Science and Biotechnology》2013,88(4):430-435

Summary

An experiment investigated the effects of two rates of irrigation, one of which reflected a substantial degree of water stress, on the mineral content, free amino acid levels and incidence of blossom-end rot (BER) in tomato (Lycopersicon esculentum Mill. `Durinta'). The plants were grown in the open with drip irrigation using saline water from a well (mean EC_w 5.2 dS m²¹). The yield per plant was higher and fewer fruit were affected by BER in the treatment involving the higher level of irrigation. The fruit of the first and fifth truss, and the leaves immediately above, were analysed for their macronutrient, micronutrient and free amino acid content. The macronutrient leaf and fruit content hardly showed any difference, only the N concentration in fruit being significantly affected in the water stressed plants, in which the levels were higher. The Ca concentration in the stylar portion of mature fruit, which is related with the incidence of BER, was not significantly affected by the level of irrigation. As regards micronutrients, only the Fe (in leaf and fruit of the first truss), Cu (in leaf of the first truss), Zn (in leaf and fruit of the first truss, and leaf of the fifth truss) and Mn (in leaf of the first truss) concentrations differed significantly. The total free amino acid leaf content was similar in both irrigation treatments. However, the total free amino acid content of fruit, significantly those of the first truss, was higher in the less irrigated treatment. The amino acids: aspartic acid (only from the first.truss), glutamic acid, proline and alanine had high concentrations in the fruit of the less irrigated plants, while the g-aminobutyric acid and phenylalanine (only from the fifth truss) concentrations were higher in fruit of the more irrigated plants.  相似文献   

Influence of water deficit and low air humidity in the nursery on survival of Rhamnus alaternus seedlings following planting     

S. Bañon  J. Ochoa  J. A. Franco  M. J. Sánchez-Blanco 《The Journal of Horticultural Science and Biotechnology》2013,88(4):518-522

Summary

The effect of irrigation and air humidity on the water relations and root and shoot growth of Rhamnus alaternus L. during the nursery phase was considered to evaluate the resulting degree of hardening obtained by these treatments. R. alaternus seedlings were pot-grown in two greenhouses of equal characteristics. In one of these greenhouses air humidity was controlled using a dehumidifying system, while in the other one the environmental conditions were not artificially modified. In each greenhouse, two irrigation treatments were used. Thus, four different treatments were applied during the nursery phase (January-May): 1) control air humidity + control irrigation; 2) control air humidity + deficit irrigation; 3) low air humidity + control irrigation; 4) low air humidity + deficit irrigation. In May, plants of all treatments were transplanted and grown in good environmental and irrigation conditions for one month (17 May–20 June), after which they received no irrigation until the end of the experiment (14 July). Low air humidity and water deficit reduced all shoot growth parameters during the nursery phase, however the root growth was not significantly affected by air humidity and even increased under the water deficit. The reduction in leaf water potential under water stress was induced by tissue dehydration since leaf turgor potential also decreased and non-osmotic adjustment was observed. The drought effects on water relations were similar in both low and high air humidity. The leaf stomatal conductance was also reduced by both types of stress, leading to a decrease in the rate of photosynthesis at the end of the nursery phase. Both water deficit and low air humidity showed their value as nursery acclimation processes, improving the survival of seedlings following transplanting and non-irrigation conditions (establishment phase). The stomatal regulation and a shift in the allocation of assimilates from shoot to root were the acclimation mechanisms showed by R. alaternus under both types of stress. The accumulated effects in low air humidity and water deficit plants could explain the highest percentage of survival at the end of the establishment period (97%) for the combined treatment.  相似文献   

Geographical pattern and ploidy levels of the weed Solanum elaeagnifolium (Solanaceae) from Argentina     

Marisel Scaldaferro  Franco Chiarini  Federico F. Santi?aque  Gabriel Bernardello  Eduardo A. Moscone 《Genetic Resources and Crop Evolution》2012,59(8):1833-1847

A total of 106 samples taken from natural Argentinean populations of the weedy Solanum elaeagnifolium (subgenus Leptostemonum) were studied cytologically to understand the impact of the different ploidy levels in its distribution and origin. Classical Feulgen staining was employed to determine mitotic chromosome numbers in all samples. 2C nuclear DNA content was determined by means of PI flow cytometry in eight samples of different ploidy levels. Principal component analysis and GIS tools were employed to compare altitude, annual precipitation and annual mean temperature among accessions. Three cytotypes were found: diploid (2n?=?24) which is widespread, tetraploid (2n?=?48) centered in western and southern Argentina, and hexaploid (2n?=?72) which predominates in central Argentina extending as well to the east. The annual precipitation is significantly different between tetraploids and hexaploids. Cx-values ranged from 1.231 to 1.275?pg, with statistical differences (of about 24.5–50.9?Mbp, p?≤?0.05) among accessions. Diploids are the most widespread cytotype and have adapted to a number of very different habitats. Tetraploids live in arid or semi-arid regions with a mean annual rainfall less than 500?mm. Hexaploids are successful in colonizing wetter areas, where no tetraploids were found. Thus, the distribution of cytotypes may be associated with habitat differences, particularly soil moisture. The observed cytotype pattern and the differences in DNA content suggest multiple places of origin for the polyploidy of S. elaeagnifolium in Argentina.  相似文献   

Imaging of exogenous lipoid pneumonia simulating lung malignancy in a dog     

Carminato A  Vascellari M  Zotti A  Fiorentin P  Monetti G  Mutinelli F 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(3):310-312

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Viral and antibody HEV prevalence in swine at slaughterhouse in Italy     

Di Bartolo I  Ponterio E  Castellini L  Ostanello F  Ruggeri FM 《Veterinary microbiology》2011,149(3-4):330-338

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Gene flow among different teosinte taxa and into the domesticated maize gene pool     

Marilyn L. Warburton  Garrison Wilkes  S. Taba  Alain Charcosset  Celine Mir  Fabrice Dumas  Delphine Madur  Susanne Dreisigacker  Claudia Bedoya  B. M. Prasanna  C. X. Xie  Sarah Hearne  Jorge Franco 《Genetic Resources and Crop Evolution》2011,58(8):1243-1261

Maize (Zea mays L. ssp. mays) was domesticated from one wild species ancestor, the Balsas teosinte (Zea mays ssp. parviglumis) about 9000 years ago. Higher levels of gene diversity are found in teosinte taxa compared to maize, following domestication and selection bottlenecks. Diversity in maize can be increased via gene flow from teosinte, which has certainly occurred from various taxa, but the rate of flow from different teosinte taxa and the final impact on maize evolution has been difficult to measure. One hundred populations from six Zea taxa, both domesticated (maize) and wild (teosinte), including domesticated landraces from Asia, Africa, and the Americas, were genotyped with 17 SSR markers using 15 individuals per population. Overall levels of diversity were high, and populations could be distinguished based on markers. Relationships between populations followed most published reports, or can now help resolve previously conflicting reports. Gene flow into maize from different teosinte groups, and gene flow between different teosintes, was estimated. Evidence for contributions from the Balsas teosintes and from Chalco teosintes (Z. mays ssp. mexicana) to the maize gene pool was found, as well as from Chalco into ssp. mexicana race ??Durango?? and Z. mays ssp. huehuetenengensis. These contributions are almost certainly the result of post-domestication (and ongoing) exchanges. This information must give more impetus to in situ conservation of teosinte species, and use of these teosintes to continue to direct the evolution of maize, especially in response to new diseases, insect pests, and other biotic and abiotic stresses.  相似文献   

Experimental model for removal of snake venom via hemoperfusion in rats     

Maximilian Estevan Oliveira  Jssica Campanholi  Roberta Lima Cavalcante  Felipe Silveira Moreno  Edson Hideaki Yoshida  Murilo Melo Juste Dini  lvio Franco de Camargo Aranha  Jos Carlos Cogo  Lourdes Dias  Stephen Hyslop  Denise Grotto  Valquíria Miwa Hanai‐Yoshida  Yoko Oshima‐Franco 《Journal of Veterinary Emergency and Critical Care》2020,30(3):286-294

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Recent rapid changes in the spatio‐temporal distribution of West Nile Neuro‐invasive Disease in Italy     

Giovenale Moirano  Lorenzo Richiardi  Mattia Calzolari  Franco Merletti  Milena Maule 《Zoonoses and public health》2020,67(1):54-61

In Italy, the first human case of West Nile Virus (WNV) infection was reported in 2008 and, since then, the number of cases has been steadily increasing. In this study, we describe the temporal and spatial pattern of WNV infection risk among humans in Italy, focusing on the human cases of West Nile Neuro‐invasive Disease (WNND) observed between 2008 and 2017. Incidence rates are estimated for each year and province under study. The incidence temporal trend is estimated using Poisson regression, and a spatio‐temporal cluster detection analysis is performed to detect high‐risk areas. In total, 231 WNND cases were notified in Italy between 2008 and 2017. The annual incidence rates increased during the study period (annual percentage change: 11.7%; 95%CI: ?0.9%; 26.1%). A geographical spread of the disease was observed during the study period throughout Northern Italy, with an increasing number of affected provinces. Provinces close to the Po River (the main river in the north of Italy) and the Oristano province (in the Sardinia Island) experienced the highest incidence rates during the study period. Our study shows a gradual, but rapid spread of WNND across Northern Italy from east to west and suggests the hypothesis that provinces close to Po River might present ecological and climatic conditions favourable to the virus circulation.  相似文献