Comparison of immunogenicity and virulence between Babesia bigemina parasites from continuous culture and from a splenectomised calf   总被引：1，自引：0，他引：1  

WK. JORGENSEN  AJ. DE. VOS  RJ. DALGLIESH † 《Australian veterinary journal》1989,66(11):371-372

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Anticoccidial efficacy of diclazuril in partridges.     

O Vanparijs  L Hermans  R Marsboom 《The Veterinary record》1991,129(15):339-340

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An unusual Actinobacillus equuli strain isolated from a rabbit with Tyzzer's disease     

Moyaert H  Decostere A  Baele M  Hermans K  Tavernier P  Chiers K  Haesebrouck F 《Veterinary microbiology》2007,124(1-2):184-186

Actinobacillus equuli was isolated in pure culture from the liver and lungs of an adult rabbit with Tyzzer's disease (Clostridium piliforme). Based on the haemolytic features on blood agar plates, a positive reaction in the CAMP-test, hydrolysis of esculin, the inability to ferment l-arabinose, tDNA-PCR and sequencing of the 16S rRNA gene, the isolate was classified as A. equuli subsp. haemolyticus biovar 1. However, the aqxA gene, characteristic for haemolytic A. equuli strains, was not detected by PCR.  相似文献   

High and low virulence Staphylococcus aureus strains in a rabbit skin infection model     

Meulemans L  Hermans K  Duchateau L  Haesebrouck F 《Veterinary microbiology》2007,125(3-4):333-340

In the present study, an in vivo rabbit skin infection model was developed to reproduce the lesions caused by high and low virulence Staphylococcus aureus strains from rabbits. "O"-shaped dermal skin lesions were induced on the shaved flanks of anaesthetised rabbits using a tattoo pin and pincers. The induced lesions on the flanks of four groups of 10 rabbits were then inoculated by topical application of 0.1 ml of 10(8)cfu S. aureus bacteria. One group was inoculated with a typical high virulence (HV) S. aureus strain from rabbits, one group received an atypical HV strain and two groups were inoculated with low virulence (LV) strains. Five animals were kept as negative controls. The development, appearance and size of abscesses were scored daily for a period of 2 weeks. The infection model showed reproducible results for the different S. aureus inoculation groups. Inoculation of the skin with the typical HV strain resulted in significantly larger abscesses than those caused by the LV strains. The atypical HV strain caused abscesses of a size intermediate to that obtained with the HV and LV strains. In rabbits infected with LV strains, most of the lesions had healed by day 14 post-inoculation. The devised infection model is able to reliably reproduce the virulence properties of HV and LV S. aureus strains.  相似文献   

Penetration of topically administered dexamethasone disodium phosphate and prednisolone acetate into the normal equine ocular fluids     

Hanneke Hermans  Els M. H. van den Berg  Inge J. M. Slenter  Dax J. C. Vendrig  Lilian J. L. de Nijs-Tjon  Johannes C. M. Vernooij  Harold Brommer  Michael H. Boevé  Ronette Gehring 《Equine veterinary journal》2022,54(5):965-972

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Actinobacillus pleuropneumoniae infections in closed swine herds: infection patterns and serological profiles     

Chiers K  Donné E  Van Overbeke I  Ducatelle R  Haesebrouck F 《Veterinary microbiology》2002,85(4):343-352

Many farrow-to-finish herds are endemically infected with Actinobacillus pleuropneumoniae. In order to control the disease efficiently, a better knowledge of the ages at which pigs become infected is necessary. Furthermore, no information is available concerning the influence of maternally derived antibodies on the colonization of the upper respiratory tract. Therefore, A. pleuropneumoniae infection patterns were studied in five farrow-to-finish pig herds (A-E) with a history of pleuropneumonia. A longitudinal study was carried out in herds A and B. In these herds, piglets from sows carrying A. pleuropneumoniae in their noses or tonsils were sampled. Nasal and tonsillar swabs as well as sera, were collected from these animals at the age of 4, 8, 12, 16 (herds A and B) and 23 weeks (herd B). At these ages other pigs from the same sows were euthanized. The lungs were macroscopically examined and samples from nose, tonsils and lungs were collected at necropsy. A cross-sectional study was performed in herds C-E. In these herds nasal and tonsillar swabs, as well as sera, were taken from 10 animals of 4, 8, 12 and 16 weeks of age. Lung, nasal and tonsillar samples were tested for the presence of A. pleuropneumoniae by routine bacteriology and PCR with mixed bacterial cultures. The sera were examined for the presence of Apx toxin neutralizing antibodies. In herd A, A. pleuropneumoniae serotype 2 and 10 strains were isolated, whereas serotype 2, 3, 5b and 8 strains were demonstrated in herd B. In most herds, A. pleuropneumoniae was detected in mixed bacterial cultures of tonsillar and/or nasal samples by PCR from the age of 4 weeks onwards. Colonization of the lungs and development of lung lesions was observed in 12- and 16-week-old animals of herd A and 23-week-old animals of herd B. In most herds, high antibody titres were detected in 4-week-old piglets. These titres decreased during the first 12 weeks of age, but thereafter, increased. It was concluded that PCR with mixed bacterial cultures from tonsillar swabs is a valuable tool for the detection of infected animals. It was also concluded that colonization of tonsils and nasal mucosae can occur in the presence of maternally derived antibodies. Infection of the upper respiratory tract without lung involvement did not result in development of Apx toxin neutralizing antibodies. Therefore, such serological assays cannot be used for the detection of subclinically infected animals.  相似文献   

Pathogenesis of infections with Salmonella enterica subsp. enterica serovar Muenchen in the turtle Trachemys scripta scripta     

Pasmans F  De Herdt P  Dewulf J  Haesebrouck F 《Veterinary microbiology》2002,87(4):315-325

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Screening for methicillin-resistant staphylococci in dogs admitted to a veterinary teaching hospital     

Vanderhaeghen W  Van de Velde E  Crombé F  Polis I  Hermans K  Haesebrouck F  Butaye P 《Research in veterinary science》2012,93(1):133-136

This study investigated the nasal carriage of methicillin-resistant staphylococci (MRS) in dogs (n=177) prior to medical examination or surgery in a veterinary teaching hospital. Nasal swab samples were collected after induction of anaesthesia and incubated overnight in salt enriched trypticase-soy broth. Cultures were analysed on two different agar media containing cefoxitin. Suspected MRS isolates were genotypically identified and characterised by antimicrobial susceptibility testing and staphylococcal cassette chromosome mec (SCCmec)-typing. Methicillin-resistant Staphylococcus aureus (MRSA) isolates were additionally characterised by spa-typing and multilocus sequence typing. The presence of Panton-Valentine leukocidin (pvl) genes was determined by PCR. MRS carriage was compared between animals with or without an infectious process. Two MRSA were isolated, both belonging to typical Belgian human hospital clones and lacking pvl. Additionally a methicillin-resistant Staphylococcus haemolyticus carrying a type V SCCmec was detected. No relationship was observed between MRS carriage and presence of infections. The results suggest that MRS are present in dogs originating from the community, albeit at a low prevalence. This could pose risks for cross contamination of dogs and their owners.  相似文献   

Prophylactic and metaphylactic antimicrobial use in Belgian fattening pig herds   总被引：1，自引：0，他引：1  

Callens B  Persoons D  Maes D  Laanen M  Postma M  Boyen F  Haesebrouck F  Butaye P  Catry B  Dewulf J 《Preventive veterinary medicine》2012,106(1):53-62

The monitoring of antimicrobial use is an essential step to control the selection and spread of antimicrobial resistance. Between January and October 2010 data on prophylactic and metaphylactic antimicrobial use were collected retrospectively on 50 closed or semi-closed pig herds. Ninety-three percent of the group treatments were prophylactic whereas only 7% were methaphylactic. The most frequently used antimicrobials orally applied at group level were colistin (30.7%), amoxicillin (30.0%), trimethoprim-sulfonamides (13.1%), doxycycline (9.9%) and tylosin (8.1%). The most frequently applied injectable antimicrobials were tulathromycin (45.0%), long acting ceftiofur (40.1%) and long acting amoxicillin (8.4%). The treatment incidences (TI) based on the used daily dose pig (UDD(pig) or the actually administered dose per day per kg pig of a drug) for all oral and injectable antimicrobial drugs was on average 200.7 per 1000 pigs at risk per day (min=0, max=699.0), while the TI based on the animal daily dose pig (ADD(pig) or the national defined average maintenance dose per day per kg pig of a drug used for its main indication) was slightly higher (average=235.8, min=0, max=1322.1). This indicates that in reality fewer pigs were treated with the same amount of antimicrobials than theoretically possible. Injectable products were generally overdosed (79.5%), whereas oral treatments were often underdosed (47.3%). In conclusion, this study shows that prophylactic group treatment was applied in 98% of the visited herds and often includes the use of critically important and broad-spectrum antimicrobials. In Belgium, the guidelines for prudent use of antimicrobials are not yet implemented.  相似文献   

In vitro susceptibility testing of Malassezia pachydermatis to gentamicin          下载免费PDF全文

Freddy A. Silva  Otilia Ferrer  Soraya Déniz  Inmaculada Rosario  Magnolia Conde‐Felipe  Esther L. Díaz  Begoña Acosta‐Hernández 《Veterinary dermatology》2017,28(4):348-e76

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