Stimulatory effect of aluminum on the growth of tea pollen tubes     

Hiromi Yokota  Ikuyo Takamura  Fumiko Ishikawa  Miwako Ohta  Shigeki Konishi 《Soil Science and Plant Nutrition》2013,59(2):457-461

It is well known that when inorganic elements such as boron (B), calcium, cobalt, and manganese are added to media in combination or alone, they stimulate the germination and/or tube growth of various kinds of pollen (Schmücker 1933; Loo and Hwang 1944; Yamada 1958; Brewbaker and Kwack 1963). Among these elements, the stimulatory effect ofB is the most effective and generally recognized. Aluminum (Al) belongs along with B to Group IIIa in the periodic table, and Fähnrich and Ultlich (1964) reported that Al inhibited the germination and growth of the pollen tube of Petunia hybrida and Antirrhinum majus. However, stimulatory effects of Al on pollen germination or pollen tube growth had not been previously reported.  相似文献   

T-type chloroplast DNA inSolarium tuberosum L. ssp.tuberosum was conferred from some populations ofS. tarijense Hawkes     

Kazuyoshi Hosaka 《American Journal of Potato Research》2003,80(1):21-32

The highly heterozygous and tetraploid nature of potato (Solanum tuberosum L. ssp.tuberosum) has hampered discovery of its wild ancestral species. Chloroplast DNA is a very reliable indicator to trace maternal ancestry of crops. Most of the common potato (grown worldwide) has unique, T-type chloroplast DNA derived from Chilean cultivated potato (both areS. tuberosum ssp.tuberosum). Analyzing seven different chloroplast DNA markers, I found all the T-type accessions of cultivated potatoes shared the same chloroplast DNA haplotype only with some accessions ofS. tarijense Hawkes and its derived hybrids. Thus, I conclude that some populations ofS. tarijense acted as the maternal ancestor of potato.  相似文献   

Genome editing of Nf1, Pten,and Trp53 in neonatal mice induces glioblastomas positive for oligodendrocyte lineage transcription factor 2     

Hiromi Yamamoto  Keisuke Yamamura  Haruka Nagasaki  Takamasa Suzuki  Fumiko Ninomiya  Kenji Matsubara  Naomoto Harada  Shuichi Ohkubo 《Journal of toxicologic pathology》2021,34(4):359

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Effects of flooding depth on growth,morphology and photosynthesis in <Emphasis Type="Italic">Alnus japonica</Emphasis> species     

Fumiko Iwanaga  Fukuju Yamamoto 《New Forests》2008,35(1):1-14

The present study deals with effects of flooding depth on growth, morphology and photosynthesis in Alnus japonica species thorough one field study and two controlled experiments. In the field study performed in Kushiro Mire, Hokkaido Island, Japan, tree heights and stem diameters decreased with an increase in water depth accompanied with the reduction of soil redox potential. In contrast, the rate of multiple stems per individual tree increased. In the controlled experiments for seedlings flooding suppressed the shoot elongation and biomass increment in roots. However, diameter increment around water levels, epicormic shoot development and adventitious root formation were enhanced in flooded seedlings. The photosynthetic rate and stomatal conductance of flooded seedlings also were lowered with an increase in flooding depth. The recovery of the reduced photosynthetic rate and stomatal conductance occurred simultaneously with the advancement of adventitious root formation in the flooded seedlings. These results indicate the importance of a series of morphological changes occurring on stems around water levels in flood tolerance in A. japonica species.  相似文献   

Ammonia volatilization from Vietnamese acid sulfate paddy soil following application of digested slurry from biogas digester     

Nakamura  Masato  Oritate  Fumiko  Yuyama  Yoshito  Yamaoka  Masaru  Dan  Nguyen Phuoc  Hanh  Dang Vu Bich 《Paddy and Water Environment》2018,16(1):193-198

Paddy and Water Environment - In some areas of Vietnam, digested slurry from biogas digesters is discharged into canals without wastewater treatment. In order to reduce environmental pollution,...  相似文献   

Harpinpsta from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity     

Fumiko TAGUCHI  Rui TANAKA  Sayuri KINOSHITA  Yuki ICHINOSE  Yoshiyuki IMURA  Salamah ANDI  Kazuhiro TOYODA  Tomonori SHIRAISHI  Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123

To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_psta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpin_psta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P. s. pv. tabaci. Received 10 August 2000/ Accepted in revised form 21 December 2000  相似文献   

Technique for screening immune-enhancing polysaccharides in food using 1,25-dihydroxyvitamin D3-differentiated HL60 cells     

Monobe M  Ema K  Kato F  Hirokane H  Maeda-Yamamoto M 《Journal of agricultural and food chemistry》2007,55(7):2543-2547

A technique for screening immune-enhancing polysaccharides in food using the phagocytotic activity of 1,25-dihydroxyvitamin D3 (VD3)-differentiated HL60 cells is presented. HL60 cells, a human acute promyelocytic cell line, can differentiate along the monocytic lineage following exposure to VD3 or phorbol-12-myristate-13-acetate (PMA). For differentiated cells along the monocytic pathway, HL60 cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 120 nM VD3 for more than 1 week. VD3-differentiated HL60 cells were seeded into 48-well plates, YG-labeled microspheres and polysaccharides were added and mixed using a plate shaker at 1100 rpm for 30 s, and then the mixture was incubated overnight at 37 degrees C in 5% CO2. The cells were fixed with 2% formaldehyde and resuspended in phosphate-buffered saline. The rate of phagocytosis was measured with a flow cytometer. VD3-differentiated cells but not non- and PMA-differentiated cells resulted in an elevation of phagocytic activity by various immune-enhancing polysaccharides in foods.  相似文献   

Prevention of immune responses to human erythropoietin in cynomolgus monkeys (Macaca fascicularis)     

Ageyama N  Hanazono Y  Shibata H  Ono F  Nagashima T  Ueda Y  Yoshikawa Y  Hasegawa M  Ozawa K  Terao K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):507-510

Genes and proteins of human origin are often administered to monkeys for research purposes, however, it can be difficult to obtain sufficient levels of the products in vivo due to immunological clearance. In this study, we showed that human erythropoietin (hEPO) induces generation of anti-hEPO antibody in cynomolgus macaques (n=2), although 92% of amino acid residues are common between the human and macaque EPO. The administered hEPO was thus eliminated from the animals. On the other hand, when an immunosuppressant, cyclosporin A (CyA), was administered (6 mg/kg) intramuscularly every other day in combination with hEPO (n=2), no anti-hEPO antibody was generated and high serum levels of hEPO were obtained during administration of hEPO, resulting in an increase in serum hemoglobin levels. No adverse effects associated with CyA were observed. Thus, CyA treatment is useful for prevention of immune responses associated with the administration of human proteins in monkeys.  相似文献   

Rhodococcus equi in the soil environment of horses in Inner Mongolia, China     

Takai S  Zhuang D  Huo XW  Madarame H  Gao MH  Tan ZT  Gao SC  Yan LJ  Guo CM  Zhou XF  Hatori F  Sasaki Y  Kakuda T  Tsubaki S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):739-742

Little is known about the distribution of Rhodococcus equi in the soil environment of native horses in China. One hundred and eight soil samples were collected from native-horse farms in the Hulun Beier grasslands of eastern Mongolia, the Xilin Goler grasslands of southern Mongolia, and Tongliao City in Inner Mongolia, China. The isolation rates of R. equi from soil samples from the Hulun Beier and Xilin Goler grasslands ranged from 25.9% to 30.0%. In contrast, isolation rates from soil samples from Tongliao City were as high as 82.3% and the mean number of R. equi in soil samples from Tongliao City was 10 times more than those of samples from the grasslands. The 488 isolates were examined using PCR for the presence of genes that encode virulence-associated 15-17 kDa antigen protein (VapA) and the 20 kDa antigen protein (VapB). All isolates were negative for virulence-associated proteins. Plasmid profiles of these avirulent isolates showed that cryptic plasmids of various sizes were present with an incidence of 13.3% to 21.5%. The results of the present study contrast with those of our recent study (J. Vet. Med. Sci. 67:611-613, 2005), in which we reported that R. equi was absent from Mongolian horses in Ulaanbaatar, Mongolia. It is suggested that the difference between the results of these two studies is due to the mobile pasturing system in Mongolia and nonmobile pasturing system in Inner Mongolia.  相似文献   

Fine mapping of foxglove aphid (Aulacorthum solani) resistance gene Raso1 in soybean and its effect on tolerance to Soybean dwarf virus transmitted by foxglove aphid     

Shizen Ohnishi  Noriyuki Miyake  Toru Takeuchi  Fumiko Kousaka  Satoshi Hiura  Osamu Kanehira  Miki Saito  Takashi Sayama  Ayako Higashi  Masao Ishimoto  Yoshinori Tanaka  Shohei Fujita 《Breeding Science》2012,61(5):618-624

Soybean dwarf virus (SbDV) causes serious dwarfing, yellowing and sterility in soybean (Glycine max). The soybean cv. Adams is tolerant to SbDV infection in the field and exhibits antibiosis to foxglove aphid (Aulacorthum solani), which transmits SbDV. This antibiosis (termed “aphid resistance”) is required for tolerance to SbDV in the field in segregated progenies of Adams. A major quantitative trait locus, Raso1, is reported for foxglove aphid resistance. Our objectives were to fine map Raso1 and to reveal whether Raso1 alone is sufficient to confer both aphid resistance and SbDV tolerance. We introduced Raso1 into cv. Toyomusume by backcrossing and investigated the degree of aphid antibiosis to foxglove aphid and the degree of tolerance to SbDV in the field. All Raso1-introduced backcross lines showed aphid resistance. Interestingly, only one Raso1-introduced backcross line (TM-1386) showed tolerance to SbDV in the field. The results demonstrated Raso1 alone is sufficient to confer aphid resistance but insufficient for SbDV tolerance. Tolerance to SbDV was indicated to require additional gene(s) to Raso1. Additionally, Raso1 was mapped to a 63-kb interval on chromosome 3 of the Williams 82 sequence assembly (Glyma1). This interval includes a nucleotide-binding site–leucine-rich repeat encoding gene and two other genes in the Williams 82 soybean genome sequence.  相似文献