羊种布鲁氏菌疫苗株PCR-RFLP鉴定方法的建立     

马晓菁  钟旗  叶锋  谷文喜  刘丽娅  马俊杰  薛晶  易新萍 《中国动物检疫》2018,35(7):91-94

为建立一种羊种布鲁氏菌Rev.1疫苗株PCR-RFLP鉴定方法,利用羊种布鲁氏菌Rev.1具有链霉素抗性的特性,选取与链霉素抗性相关编码核糖体蛋白S12的rps L基因为模板设计引物,经PCR扩增后,将产物进行Nci I酶切,发现羊种布鲁氏菌疫苗株Rev.1出现约500 bp条带,而不具有链霉素抗性的羊种布鲁氏菌株16M则出现384 bp条带。结果表明,PCR-RFLP方法能够用于羊种布鲁氏菌Rev.1疫苗株的鉴定,这为今后区分羊种布鲁氏菌疫苗株Rev.1免疫与野株感染提供了基础。  相似文献   

Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning     

TAN Xiao  YANG Da-qian  ZHANG Hai-li  JIANG Jing  CHEN Yan  ZHANG Zhi-gang 《中国畜牧兽医》2016,43(6):1506-1511

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.  相似文献   

北京市屋顶绿化滞尘及不同材质热辐射效应测定     

李连龙  雷赛姣  桂琳丽 《河北林业科技》2017,(1)

为探讨屋顶绿化的滞尘效应和不同材质的热辐射效应,试验进行了屋顶滞尘试验研究。滞尘测试结果表明:花园式屋顶绿化滞尘量平均为25.1349g/m2,比简单式屋顶绿化滞尘率高约10个百分点。热辐射对比测定得出:裸露屋顶热像值明显高于绿化屋顶,屋顶绿化是缓解城市热岛效应的关键手段。屋顶单一植物绿化,其热像值高于多种植物复层结构群落式绿化;佛甲草等景天属植物的热像值高于草坪地被植物,草坪地被植物的热像值高于乔灌木;植物花体部分的热像值高于叶体部分;枯死植物热像值明显高于活体植物;呼吸蒸腾作用弱的植物热像值高于呼吸蒸腾作用旺盛的植物。  相似文献   

Comparative pharmacokinetics of oxytetracycline in blunt-snout bream (Megalobrama amblycephala) with single and multiple-dose oral administration     

Ru-Qin Li  Yu-Wei Ren  Jing Li  Can Huang  Jun-hui Shao  Xiao-Xuan Chen  Zhi-Xin Wu 《Fish physiology and biochemistry》2015,41(3):803-809

  相似文献   

豫南地区野生地被植物资源现状调查与研究   总被引：1，自引：0，他引：1  

尹娟  王晶 《黑龙江农业科学》2015,(3)

豫南地区野生地被植物资源丰富,为了在城市绿化中引入新的具有利用价值的种类,对以信阳为中心的河南省南部具有典型立地条件的区域的野生地被植物资源进行了调查。结果表明:筛选统计出野生地被植物56科,359属,822种,其中具有较大开发利用价值的50种。通过对50种野生地被植物中的15种进行利用价值研究得出,随着植物的生态适应性的增强,该种植物同时观赏价值、经济价值也相对增高,在园林应用中可利用价值也越大。  相似文献   

菱传粉生物学研究     

李双梅  柯卫东  彭静  黄新芳  李明华 《中国蔬菜》2019,1(8):54-58

以6 个菱品种为试材，对花器形态特征、群体花期及单花开花动态、开花时间、柱头可授性、花粉活力、杂交指数 及传粉形式等进行观察测定。结果表明：菱花单生，两性，单花花期15 h，群体花期在5～10 月，盛花期在7～8 月；不同 品种在一天中的开花高峰期存在差异；柱头可授期主要在萼片开裂后2 h 内，结实率在50% 以上；花粉在自然授粉条件下活 力可保持7.5 h；以自花授粉为主，同时存在一定程度的无融合生殖和少量异花授粉。  相似文献   

Research Progress on Influence Factors of Mutton Quality     

LIANG Jing  ZHANG Wen-ju  WANG Bo 《中国畜牧兽医》2016,43(5):1250-1254

Meat quality means the characteristics such as appearance is related to palatability in fresh meat or manufactured meat, it includes meat color, meat structure, hardness, marbling, water holding capacity and so on.The physical characteristics of mutton quality determines the consumer acceptability of meat and there is a close relationship between chemical composition of mutton quality and nutrient substance.With the improvement of living standards, consumers are increasingly high demand for mutton.From quantitative to qualitative change, we need to continuously improve mutton quality.At present, the production of high quality animal products has become a hot topic.This paper is a summary of the effects of breed, gender, age, environment and feed nutrition on mutton quality, and aims to provide a scientific basis for improving of mutton quality with nutritional regulation measures.  相似文献   

The localization and expression of epidermal growth factor and epidermal growth factor receptor in bovine ovary during oestrous cycle     

Yuru Luo  Ruiqi Zhang  Jing Gao  Yali Wang  Weimin Zhang  Suzhu Qing 《Reproduction in domestic animals》2020,55(7):822-832

Epidermal growth factor (EGF) is one of the important regulatory factors of EGF family. EGF has been indicated to effectively inhibit the apoptosis of follicular cells, to promote the proliferation of granulosa cells and the maturation of oocytes, and to induce ovulation process via binding to epidermal growth factor receptor (EGFR). However, little is known about the distribution and expression of EGF and EGFR in cattle ovary especially during oestrous cycle. In this study, the localization and expression rule of EGF and EGFR in cattle ovaries of follicular phase and luteal phase at different time points in oestrous cycle were investigated by using IHC and real-time qPCR. The results showed that EGF and EGFR in cattle ovary were mainly expressed in granulosa cells, cumulus cells, oocytes, zona pellucida, follicular fluid and theca folliculi externa of follicles. The protein and mRNA expression of EGF/EGFR in follicles changed regularly with the follicular growth wave both in follicular and in luteal phase ovaries. In follicular phase ovaries, the protein expression of EGF and EGFR was higher in antral follicles than that of those in other follicles during follicular growth stage, and the mRNA expression of EGFR was also increased in stage of dominant follicle selection. However, in luteal phase ovaries, the growth of follicles was impeded during corpus luteum development under the action of progesterone secreted by granular lutein cell. The mRNA and protein expressions of EGF and EGFR in ovarian follicles during oestrous cycle indicate that they play a role in promoting follicular development in follicular growth waves and mediating the selection process of dominant follicles.  相似文献   

鲜食型超甜玉米新品种朝甜603   总被引：5，自引：0，他引：5  

王颢  杨吉德  展宗冰  张雪琴  闫秀霞  王子玉  王静  杨杰 《中国蔬菜》2015,1(7):87

<正>朝甜603为早熟鲜食加工兼用型超甜玉米新品种,鲜穗籽粒排列整齐、致密、金黄色,饱满度好、甜度高、皮薄无渣适口性好;植株耐热耐湿,抗倒伏、抗病性强。朝甜603( 原名超甜603) 是以E09Z28为母本,E09Z31为父本配制而成的超甜玉米杂交种。母本E09Z28是以SHE-03与金甜甜为基础材料杂交后经多代自交系选而成,父本E09Z31是以RDE-05与金凤超甜玉米(05)为基础材料杂交后  相似文献   

近32年来新疆地区植被覆盖的时空变化   总被引：2，自引：0，他引：2  

许玉凤  杨井  陈亚宁  杨雅雪 《草业科学》2015,32(5):702-709

基于拟合的长时间序列数据监测新疆植被时空动态变化。结果表明,近32年来,新疆植被总体呈现增加趋势,但存在明显的阶段变化和空间差异;植被生长季NDVI值西部和西北部高,东部和东南部低;1982-2013年间新疆植被改善面积大于退化面积,绿洲农田植被覆盖区改善明显、草原区退化严重;整个研究区域的植被重心从北向南移动,天山以北的植被重心呈现西南-东北方向移动,天山以南呈现近似东西方向的移动。  相似文献