西瓜核型雄性不育两用系POD同工酶研究   总被引：1，自引：1，他引：1  

马建祥  张显  张勇  杨建强 《中国瓜菜》2005,(4):30-32

用PAGE(聚丙烯酰胺凝胶)电泳技术,研究了西瓜核型雄性不育两用系不同器官的POD(过氧化物酶)同工酶酶谱差异。试验结果表明,不育株与可育株主要差异在雄花蕾,表现为不育株较可育株多2条活性强的酶带;其他器官差异小,仅表现为酶谱带强弱不同。并对POD同工酶与雄性不育性的相关性进行了研究。  相似文献   

不同砧木嫁接网纹甜瓜试验     

王毓洪  李林章  黄芸萍  孟秋峰  邵必丰  王祥兆  何智龙 《中国瓜菜》2005,(6):25-27

为预防网纹甜瓜枯萎病等土传病害,以新土佐、灰甘瓜、全能铁甲南瓜,甬砧3号甜瓜为砧木,以当地主栽的拿玻里网纹甜瓜为接穗,进行嫁接试验。结果表明:拿玻里网纹甜瓜嫁接后,植株的抗病性有了显著提高,对枯萎病的防效高达100%,对白粉病的防效也有所提高,综合抗性增强。其中甬砧3号在4个参试砧木品种中,与接穗拿玻里网纹甜瓜的亲和性最好,抗病性提高明显,品质也较自根苗好。  相似文献   

L-肉碱在水产养殖中的应用     

王优军  李勇  蒋克勇 《中国饲料》2005,(16):20-21

L-肉碱是一种具有多种生理功能的新型添加剂。在水产饲料中添加适量L-肉碱不但可以提高饲料利用效率、降低饵料系数、加快水产动物的生长,还能节约蛋白质日粮,改善肉质。  相似文献   

不同生长年限王草的营养价值研究   总被引：2，自引：0，他引：2  

陈勇  甄莉  罗富成  毛华明  赵雪妮  左应梅 《中国饲料》2005,(16):30-31

本文对生长第1、2、3年的王草在100cm高度时刈割的营养成分及其体外干物质、有机物降解率进行分析研究。结果表明:不同生长年限王草第1年的粗蛋白质显著高于第2、3年(P<0.05),而中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)显著低于第3年(P<0.05)。干物质、有机物的降解率第1年最高,第3年的最低,但差异不大。  相似文献   

八珍汤对乳牛产后免疫状态的影响   总被引：1，自引：0，他引：1  

黄群山  戴承胜刘宇 曾中良 《中国兽医科技》2005,35(11):912-916

为了探索提高乳牛产后期免疫状态的新方法，对试验乳牛分4组（产前第30d至产前第1d灌喂组、产前第15d至产后第15d灌喂组、产后第1d至第30d灌喂组、不灌喂中药对照组）灌喂中药八珍汤，检测产后期淋巴细胞及其亚群数量和淋巴细胞增殖活化功能。结果发现，产前组CD3细胞数量在产后第1d升高；产前产后组CD3、CD4和CD8细胞数量在产后第1d和第15d升高；产后组CD3细胞数量在产后第15d和第30d升高，CD4细胞数量也在产后第30d升高。淋巴细胞对ConA的反应能力，产前产后组在产后第1～30d明显提高，产后组在产后第15d和第30d明显提高。各组乳牛产后期CD21细胞数量的变化相近。结果表明，从产前第15d开始到产后第15d每日喂八珍汤，能明显提高乳牛产后期T细胞及其亚群数量和增加淋巴细胞增殖活化功能，而对B细胞数量增加的作用不明显。  相似文献   

园林布局中必然性和偶然性要素的辩识与运用     

罗志军  杨勇 《四川畜牧兽医学院学报》2005,3(4):165-166

阐释了园林构成要素中的必然性要素和偶然性要素及其相互关系与运用意义；以昆明世博园中“齐鲁园”为例，辩识了“齐鲁园”中必然性与偶然性园林要素，分析了诸要素在特定园林环境布局中的运用。  相似文献   

Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment     

HUANG Feng  WAN Yong-ling  WU Da-long  LV Huan-zhang  GUO Jun-hua 《园艺学报》2003,19(8):1045-1048

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.  相似文献   

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese     

ZHOU Shu-lu  YE Ren-gao  LIU Xiao-bo  ZHANG Hong  XU Han-shi  DU Yong  LI You-ji  YANG Nian-sheng  YANG Xiao  YU Xue-qing 《园艺学报》2003,19(6):782-785

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.  相似文献   

Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat     

ZHANG En-guang  ZHANG Huai-qin  LIN Jie  HUANG Wei-jian  LI Shang-gong  XU Li-xin 《园艺学报》2003,19(5):653-656

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.  相似文献   

The basic mechanism of increased microvascular permeability     

ZHAO Ke-sen  HUANG Qiao-bing 《园艺学报》2003,19(4):549-553

The increased microvascular permeability appears mainly in venule during inflammation, shock, and burns. Endothelial cells play an important role in venule permeability enhancement. There are two kinds of pathway for macromolecule extravasation. One is paracellular pathway and another is transcellular pathway, which are related to the formation of endothelial gap or transcellular openings seperately. The alteration of intercellular related protein, such as occludin, claudin, zona occludens (ZO), junctional adhesion molecule (JAM), VE cadherin, catenin, integrin, etc, and the alteration of endothelial cytoskeleton, such as rearrangement of actin filament, formation of stress fiber and focal adhesion, etc, involve in the pathogenesis of increased microvascular permeability.  相似文献