Identification of human glucocorticoid receptor complementary DNA clones by epitope selection   总被引：39，自引：0，他引：39  

C Weinberger  S M Hollenberg  E S Ong  J M Harmon  S T Brower  J Cidlowski  E B Thompson  M G Rosenfeld  R M Evans 《Science (New York, N.Y.)》1985,228(4700):740-742

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Hindbrain GABA receptors influence parasympathetic outflow to the stomach     

D J Williford  H S Ormsbee  W Norman  J W Harmon  T Q Garvey  J A DiMicco  R A Gillis 《Science (New York, N.Y.)》1981,214(4517):193-194

Blockade of gamma-aminobutyric acid receptor function by direct microinjection of bicuculline into the nucleus ambiguous in cats produced a marked increase in gastric motility which was mediated by the vagus nerve. This effect was reversed by muscimol. These data indicate that the nucleus ambiguous may be an important brain site influencing gastric function and that the neurotransmitter controlling parasympathetic overflow from this nucleus to the stomach is gamma-aminobutyric acid.  相似文献   

An assessment of potential pesticide transmission,considering the combined impact of soil texture and pesticide properties: A meta-analysis     

John McGinley  Jenny Harmon O’Driscoll  Mark G. Healy  Paraic C. Ryan  Per Eric Mellander  Liam Morrison  Oisin Callery  Alma Siggins 《Soil Use and Management》2022,38(2):1162-1171

Pesticides are widely employed as a cost-effective means of reducing the impacts of undesirable plants and animals. The aim of this paper is to develop a risk ranking of transmission of key pesticides through soil to waterways, taking into account physico-chemical properties of the pesticides (soil half-life and water solubility), soil permeability, and the relationship between adsorption of pesticides and soil texture. This may be used as a screening tool for land managers, as it allows assessment of the potential transmission risks associated with the use of specified pesticides across a spectrum of soil textures. The twenty-eight pesticides examined were differentiated into three groups: herbicides, fungicides and insecticides. The highest risk of pesticide transmission through soils to waterways is associated with soils containing <20% clay or >45% sand. In a small number of cases, the resulting transmission risk is not influenced by soil texture alone. For example, for Phenmedipham, the transmission risk is higher for clay soils than for silt loam. The data generated in this paper may also be used in the identification of critical area sources, which have a high likelihood of pesticide transmission to waterways. Furthermore, they have the potential to be applied to GIS mapping, where the potential transmission risk values of the pesticides can be layered directly onto various soil textures.  相似文献   

Questions about forensic science     

Harmon R  Budowle B 《Science (New York, N.Y.)》2006,311(5761):607-10; author reply 607-10

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A variant of the HTRA1 gene increases susceptibility to age-related macular degeneration   总被引：1，自引：0，他引：1  

Yang Z  Camp NJ  Sun H  Tong Z  Gibbs D  Cameron DJ  Chen H  Zhao Y  Pearson E  Li X  Chien J  Dewan A  Harmon J  Bernstein PS  Shridhar V  Zabriskie NA  Hoh J  Howes K  Zhang K 《Science (New York, N.Y.)》2006,314(5801):992-993

Age-related macular degeneration (AMD) is the most common cause of irreversible vision loss in the developed world and has a strong genetic predisposition. A locus at human chromosome 10q26 affects the risk of AMD, but the precise gene(s) have not been identified. We genotyped 581 AMD cases and 309 normal controls in a Caucasian cohort in Utah. We demonstrate that a single-nucleotide polymorphism, rs11200638, in the promoter region of HTRA1 is the most likely causal variant for AMD at 10q26 and is estimated to confer a population attributable risk of 49.3%. The HTRA1 gene encodes a secreted serine protease. Preliminary analysis of lymphocytes and retinal pigment epithelium from four AMD patients revealed that the risk allele was associated with elevated expression levels of HTRA1 mRNA and protein. We also found that drusen in the eyes of AMD patients were strongly immunolabeled with HTRA1 antibody. Together, these findings support a key role for HTRA1 in AMD susceptibility and identify a potential new pathway for AMD pathogenesis.  相似文献   

Nutritional evaluation of poultry by-product meal as a protein source for ruminants: small intestinal amino acid flow and disappearance in steers.     

D W Bohnert  B T Larson  M L Bauer  A F Branco  K R McLeod  D L Harmon  G E Mitchell 《Journal of animal science》1999,77(4):1000-1007

Six Angus steers (260+/-4 kg initial BW) fitted with ruminal, duodenal, and ileal cannulas were used in a 6 x 6 Latin square design to evaluate the effect of feeding poultry by-product meal (PBM) on small intestinal flow and disappearance of amino acids. The diets were provided at 2% of BW on a DM basis, formulated to contain 11.5% CP, and consisted of 49% corn silage, 36% cottonseed hulls, and 15% supplement on a DM basis. Supplements were formulated to contain 37% CP with sources of supplemental N being soybean meal (100% SBM) and 0, 25, 50, 75, and 100% PBM, with urea used to balance for N. Duodenal flow of all amino acids increased linearly (P < .07) as PBM increased in the diet and, except for His, increased (P < .09) for 100% PBM compared with 100% SBM. Similar results were observed for duodenal flow of nonbacterial amino acids, which linearly increased (P < .05) with PBM and were greater (P < .05) for 100% PBM than for 100% SBM. Soybean meal increased (P < .09) the duodenal flow of nonbacterial Lys compared with 0% PBM, and 0% PBM increased (P < .04) flow of Val, Ala, and Pro compared with 100% SBM. Duodenal bacterial essential, nonessential, and total amino acid flows were not affected (P > .80) by PBM; however, they were greater (P < .02) for 100% SBM than for 100% PBM. In addition, nonessential and total bacterial amino acid flows were increased (P < .06) for 100% SBM compared with 0% PBM. Small intestinal disappearance of Lys and Pro increased linearly (P < .09) as PBM increased, and 100% PBM increased (P < .07) disappearance of Arg and Ala compared with 100% SBM. Supplemental N source had no effect (P > .31) on apparent small intestinal disappearance of essential, nonessential, and total amino acids. These data suggest that when PBM, SBM, and urea were used as sources of supplemental N, the daily disappearance of amino acids from the small intestine of steer calves consuming a corn silage- and cottonseed hull-based diet was similar.  相似文献