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排序方式: 共有246条查询结果,搜索用时 31 毫秒
81.
T G Nagaraja T B Avery S J Galitzer D L Harmon 《American journal of veterinary research》1985,46(12):2444-2452
Salinomycin, a new ionophore antibiotic, was tested and compared with lasalocid and monensin for preventing experimentally induced lactic acidosis. Five rumen-fistulated adult cattle were used in a 5 X 5 Latin square design, and the treatments were as follows: no treatment (control), 0.11 mg of salinomycin/kg of body weight (S1), 0.22 mg of salinomycin/kg (S2), 0.66 of lasalocid/kg, and 0.66 mg of monensin/kg. Acidosis was induced by intraruminal administration of a ground corn-corn starch mixture (50:50, 12.5 g/kg) once a day for up to 4 days. Antibiotics were administered along with grain-starch mixture. Rumen and blood samples were obtained before and at 6, 12, and 24 hours after each carbohydrate-antibiotic dosing to monitor acid-base status. Control and S1-treated cattle became ruminally acidotic within 54 hours, whereas cattle treated with S2, lasalocid, and monensin resisted acidosis for up to 78 hours after dosing. Cattle treated with S2, lasalocid, or monensin had higher rumen pH and lower L(+)- and D(-)-lactate concentrations than did control or S1-treated cattle. Rumen pH decrease to below 5.0 in S2-, lasalocid-, and monensin-treated cattle was not due to lactic acid, but to increased production of volatile fatty acids. Rumen propionate proportion increased initially in antibiotic-treated cattle, but after 48 hours, butyrate proportion increased significantly. Despite low rumen pH and high lactate concentration, lacticacidemia was not evident, and the systemic acid-base disturbance was mild in control cattle.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
82.
Steam-rolled wheat diets for finishing cattle: effects of dietary roughage and feed intake on finishing steer performance and ruminal metabolism 总被引:2,自引:0,他引:2
K K Kreikemeier D L Harmon R T Brandt T G Nagaraja R C Cochran 《Journal of animal science》1990,68(7):2130-2141
Two experiments were conducted to determine the influence of dietary roughage concentration and feed intake on finishing steer performance and ruminal metabolism. In Exp. 1, 126 steers (334 kg) were used in a completely randomized design and fed (120 d) diets of steam-rolled wheat without roughage or containing 5, 10 or 15% roughage (50% alfalfa hay:50% corn silage). Steers fed 5 or 10% roughage gained faster (quadratic, P less than .05) and were more efficient (quadratic, P less than .05) than steers fed 15% or no roughage. In Exp. 2, six ruminally cannulated steers (447 kg) were used in a 6 x 6 latin square design and fed (twice daily) diets of steam-rolled wheat without roughage or containing 5 or 15% alfalfa hay at twice or three times NE required for maintenance. Increasing dietary roughage increased (linear, P less than .01) ruminal liquid passage 38%, indigestible ADF passage 63%, Yb-labeled wheat passage 75% and fiber fill 31%. The rate of in situ starch digestion tended to increase (linear, P = .16), and ruminal VFA concentration was 40 mM higher (P less than .01) at 4 h after feeding with increased roughage. Increased feed intake increased (P less than .05) ruminal starch fill, fiber fill, liquid fill and liquid passage 23%, Yb-labeled wheat passage 50% and Dry-labeled hay passage 20%. It reduced protozoa five- to sixfold (P less than .01) but doubled total bacterial counts (P less than .01). Ruminal NH3N was lower (P less than .01) and total VFA concentration was 50 mM higher (P less than .01) at 4 h after feeding. The acetate:propionate ratio was reduced from 2.3 to 1.3 (P less than .01) with increased intake. Adding roughage to a steam-rolled wheat diet increased passage and tended to increase rate of starch digestion; increased feed intake with its associated effects on ruminal fill and passage dramatically shifted the microbial population and fermentation end products. 相似文献
83.
R M Cleale R A Britton T J Klopfenstein M L Bauer D L Harmon L D Satterlee 《Journal of animal science》1987,65(5):1319-1326
Non-enzymatic browning was tested as a means of increasing ruminal escape of soybean meal N. Soybean meal was treated with xylose (3 mol/mol SBM-lysine), sodium hydroxide (pH 8.5) and enough water to achieve an 83% dry matter mixture and then heated at 150 C for 30 min (XTS-30). Trial 1 evaluated ruminal escape of N from XTS-30 compared with commercial soybean meal (CS) or urea (U) in a replicated 3 X 3 Latin square design using six duodenally cannulated Angus X Hereford steers (24.7 kg). Duodenal flow of dietary N was higher (P less than for steers fed XTS-30 (47.9 g/d) than for steers fed CS (39.5 g/d). The ruminal escape estimate for XTS-30 (33.7%) was higher (P less than .10) than CS (13.1%), whereas total tract apparent N digestibility was not different among treatments. In trial 2, net portal absorption of alpha-amino N was measured in Finnsheep X Suffolk ram lambs (24.7 kg) fed U, CS or XTS-30 in a 3 X 3 Latin square design. Portal blood flow was measured by primed, continuous infusion of para-aminohippuric acid. Portal blood flow was lower (P less than .05) for U.fed lambs than for lambs fed CS or XTS-30, and tended to be lower for lambs fed CS than those fed XTS-30. Net portal absorption of alpha-amino N tended to be lowest for lambs fed U (281 mmol/d) and highest for lambs fed XTS-30 (578 mmol/d). The results are interpreted to show that non-enzymatic browning increased flow of soybean meal N to the intestine. 相似文献
84.
Cholesterol concentration of longissimus muscle, subcutaneous fat and serum of two beef cattle breed types 总被引:2,自引:0,他引:2
The cholesterol concentration of uncooked and cooked longissimus muscle, uncooked subcutaneous fat and serum, as affected by days on feed, breed type and sex class in a factorial arrangement, and the relationship between serum cholesterol and tissue cholesterol were studied. Days on feed, breed type and sex class had no effect (P greater than .05) on tissue cholesterol concentrations. Overall least-squares means for uncooked and cooked longissimus muscle and subcutaneous fat were 63.32, 80.27 and 98.90 mg of cholesterol/100 g of tissue, respectively. Cholesterol concentration was 26.8% higher in cooked vs uncooked muscle and 56.2% higher in uncooked subcutaneous fat compared with uncooked muscle. Serum cholesterol concentration was lower (P less than .05) for fullblood Chianinas and steers (122.6 and 133.9 mg/dl) compared with crossbreds and heifers (162.3 and 151.0 mg/dl), respectively. Serum cholesterol concentration showed a cubic response (P less than .05) over days on feed; it was lowest at 0 d (79.6 mg/dl), increased up to 77 d (156.3 mg/dl), leveled off (154.7 mg/dl), then increased again (179.3 mg/dl). Serum cholesterol had low and insignificant correlation coefficients with all tissue cholesterol concentrations (r = .08 to .22; P greater than .05). Results indicate that muscle and fat tissue cholesterol concentrations do not vary in response to the differences in breed type, sex class or time on feed represented in the present study and thus may be an inherent characteristic dependent upon quantitative needs for cellular membrane functions. However, serum cholesterol concentration was affected by treatments evaluated in this study and may be related to factors affecting lipid metabolism. 相似文献
85.
B G Harmon S G Cornelius J Totsch D H Baker A H Jensen 《Journal of animal science》1974,39(4):699-702
86.
Effect of energy source fed to sows during late gestation on neonatal blood metabolite homeostasis, energy stores and composition 总被引:3,自引:0,他引:3
M D Newcomb D L Harmon J L Nelssen A J Thulin G L Allee 《Journal of animal science》1991,69(1):230-236
Four experiments utilizing 49 primiparous and 34 multiparous sows were conducted to evaluate the effect of feeding either starch, soybean oil or medium-chain triglyceride (MCT) during late gestation (d 100 to parturition) on neonatal fasting blood glucose homeostasis (Exp. 1 and 2), d-100 maternal circulating insulin, glucagon and glucose concentrations (Exp. 3 and 4), neonatal energy stores (Exp. 3 and 4) and colostrum composition (Exp. 1, 2, 3 and 4). In all cases, sows were fed 7.2 Mcal/d of a diet that supplied 279 g of protein daily. During fasting, pigs from MCT-fed dams had higher blood glucose concentrations (P less than .05) than pigs from starch-fed dams. No differences between treatments were observed for total carcass fat, liver glycogen or carcass glycogen of pigs killed prior to suckling. In general, colostrum fatty acid profiles were influenced by diet; however, the shifts in fatty acids differed for experiments. Starch-fed sows had higher blood insulin and lower blood glucagon (P less than .05) than lipid-supplemented sows; however, sow blood glucose remained constant across treatments. This study indicates that any positive effects of feeding soybean oil to sows during late gestation are most likely mediated by a change in fatty acid composition of colostrum; any beneficial properties of MCT feeding most likely are mediated through improved blood glucose maintenance of the neonate when it is subjected to fasting conditions. 相似文献
87.
D L Harmon K L Gross C R Krehbiel K K Kreikemeier M L Bauer R A Britton 《Journal of animal science》1991,69(10):4117-4127
Twenty calves (7 mo old) were blocked by breed, sex, and weight into five groups of four calves and randomly assigned to either a 90% forage (alfalfa) or a 90% grain (50% sorghum and 50% wheat) diet fed at one (1M) or two (2M) times NEm for 140 d. Samples of ruminal epithelial tissue were collected from the anterior ventral sac, and papillae were cut free by hand and used for in vitro incubations and acyl-CoA synthetase assays. Substrates were acetate (90 mM), propionate (60 mM), butyrate (30 mM), and glucose (20 mM). Net productions of beta-hydroxybutyrate and acetoacetate from acetate were greater (P less than .01) with the 2M feeding; however, 14CO2 production from acetate was greater (P less than .05) with the grain diet. Net production of lactate (P = .09) and pyruvate (P less than .01) from propionate increased with the 2M feeding, whereas net lactate production from glucose decreased (P less than .01). Uptakes of VFA were similar with 1M and 2M feeding and were about 10-fold greater than uptakes of glucose. Production of 14CO2 from propionate was two- to fivefold greater than from acetate, butyrate, or glucose. Oxygen consumption was greater (P less than .01) with 2M feeding and unaffected by substrate. Activities of butyryl-CoA synthetase (nmol.mg of tissue-1.h-1) were greater (P less than .05) for animals consuming the forage diets. Addition of butyrate inhibited acetyl- and propionyl-CoA synthetase activity by 63 and 92%, respectively for all dietary treatments. Overall, metabolism of ruminal epithelial tissue tended to increase with the 2M feeding. Influence of dietary forage content on metabolism and activity of acyl-CoA synthetases was minimal, but the high-forage diet caused slight increases. 相似文献
88.
Two experiments were conducted to investigate the influence of dietary concentrate level and iv lactate infusion on the urinary excretion and balance of Ca, Mg and P in lambs. In Exp. 1, six ruminal fistulated, crossbred wethers (25 kg) were fed diets of 0, 50 and 70% concentrate for 5-d periods (periods 1, 2 and 3, respectively) followed by 25 d on a 90% concentrate diet (periods 4, 5 and 6, respectively). Collections were made for all days except 6 to 10 and 16 to 20 on the 90% concentrate diet. Dry matter intakes increased with each increase in dietary concentrate until the initial period of 90% concentrate (period 4) when intakes were lowest. Intakes increased (P less than .05) during the latter two periods of 90% concentrate feeding compared with period 4. Rumen fluid pH decreased and rumen L (+) lactate increased with increasing concentrate intake. Blood pH and bicarbonate both decreased with increasing concentrate intake indicating a mild disturbance in acid-base balance. Plasma concentrations of Ca, Mg and P decreased with increased concentrate intake and were elevated (P less than .05) for periods 5 and 6 compared with period 4. Plasma alkaline phosphatase activity and urinary hydroxyproline excretion both increased (P less than .05) for periods 5 and 6. Four of the six animals were in negative Ca balance for the initial period of 90% concentrate feeding, but showed highest Ca retentions for periods 5 and 6. Magnesium and P balance appeared unaffected by increased concentrate intake. Disturbances in Ca metabolism appeared to be short-term and nondetrimental; the animals responded with increased growth and Ca retentions once adjusted to the high concentrate diet. In Exp. 2, four Hampshire ewes (32 kg) were used in a 4 X 4 Latin square design with treatments being saline (.9% NaCl, w/v), L (+) lactate, D (-) lactate and D, L-lactate infused iv in a saline solution to supply .6 mM/kg body weight of each isomer in 15 min. Plasma and urine samples were taken 0, .5, 1, 1.5, 3, 6 and 12 h from the start of infusion. Total urinary excretion of Ca (P less than .04) and Mg (P less than .02) were elevated for all lactate infusions as compared with saline. Total P excretion was greater (P = .06) for all lactate infusions compared with saline and was increased (P less than .05) for the D, L-lactate treatment as compared with the D- and L-lactate treatments.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
89.
90.
Glanville T. D. Ahn H. K. Richard T. L. Shiers L. E. Harmon J. D. 《Water, air, and soil pollution》2009,204(1-4):285-297
Water, Air, & Soil Pollution - The variations of tropospheric ozone levels was assessed for the first time in the Boukornine National Park (N.E. Tunisia) by detection of leaf injury development... 相似文献