Severe dietary lysine restriction affects growth and body composition and hepatic gene expression for nitrogen metabolism in growing rats     

D.‐H. Kwon  J. J. Bong  J. Y. Jeong  Y. S. Nam  M. S. Lee  X. Liu  M. Baik 《Journal of animal physiology and animal nutrition》2014,98(1):149-157

Dietary lysine restriction may differentially affect body growth and lipid and nitrogen metabolism, depending on the degree of lysine restriction. This study was conducted to examine the effect of dietary lysine restriction on growth and lipid and nitrogen metabolism with two different degree of lysine restriction. Isocaloric amino acid–defined diets containing 1.4% lysine (adequate), 0.70% lysine (50% moderate lysine restriction) and 0.35% lysine (75% severe lysine restriction) were fed from the age of 52 to 77 days for 25 days in male Sprague–Dawley rats. The 75% severe lysine restriction increased (p < 0.05) food intake, but retarded (p < 0.05) growth, increased (p < 0.05) liver and muscle lipid contents and abdominal fat accumulation, increased (p < 0.05) blood urea nitrogen levels and mRNA levels of the serine‐synthesizing 3‐phosphoglycerate dehydrogenase gene, but decreased (p < 0.05) urea cycle arginase gene mRNA levels. In contrast, the 50% lysine restriction did not significantly (p > 0.05) affect body growth and lipid and nitrogen metabolism. Our results demonstrate that severe 75% lysine restriction has detrimental effects on body growth and deregulate lipid and nitrogen metabolism.  相似文献   

Density calculation of wood by portable X-ray tube with consideration of penetrating depth     

Chul-Ki Kim  Jung-Kwon Oh  Jung-Pyo Hong  Jun-Jae Lee 《Journal of Wood Science》2014,60(2):105-110

A portable X-ray apparatus generates soft X-rays which have a continuous wavelength (wide range). When using continuous wavelength X-rays, the mass attenuation coefficient of the soft X-rays is changed as the penetrating depth in wood increases, unlike monochromatic X-rays which are usually used for medical purposes. In safety inspections of historic buildings, penetrating depth varies in an X-ray radiograph. Computerized tomography (CT) is a powerful tool that helps determine the density information of the inner sections of buildings. Because only portable X-rays can be used in historic buildings and the penetrating depth can vary, the mass attenuation coefficient of wood according to penetrating depth needs to be investigated. Therefore, in this study, we developed a statistical method which takes into account the influence of the penetrating depth on a density calculation made by a portable X-ray apparatus. X-ray tests were conducted on wood specimens of various depths. From the results, a statically determined mass attenuation coefficient (SMAC) ( $ \mu = - 0.214\ln (t) + 0.7251 $ ), which is the equation of mass attenuation coefficient according to the penetrating depth in wood, was derived to convert radiographs to density radiographs. All projections were converted into density profiles using two methods, average mass attenuation coefficient and SMAC. Based on the density profile for each projection, a density distribution of a cross-section was reconstructed by filtered back projection. Compared with the cases using a consistent mass attenuation coefficient, SMAC provided much higher precision in density prediction than the average mass attenuation coefficient.  相似文献   

Effect of Daily Feeding Ratio on Growth and Body Composition of Subadult Olive Flounder, Paralichthys olivaceus, Fed an Extruded Diet during the Summer Season     

Sung Hwoan  Cho  Sang-Mok  Lee  Byum Hee  Park  Sung Choon  Ji  Cheol Young  Choi Jin Hwan  Lee Yi Cheong  Kim Jong Ha  Lee Sung-Yong  Oh 《Journal of the World Aquaculture Society》2007,38(1):68-73

A 10‐wk feeding trial to determine the effect of daily feeding ratio on growth and body composition of subadult olive flounder fed the extruded pellet (EP) was performed during the summer season. Thirteen flounder (an initial body weight of 319 g) per tank were distributed into fifteen 500‐L flow‐through tanks. Five treatments of feeding ratio in 5% decrement were prepared in triplicate: 100 (satiation), 95, 90, 85, and 80% of satiation. Fish in the control group (100% of satiation) were hand‐fed to apparent satiation twice a day. Then, feed allowance in the rest of the four groups was determined based on average feed consumption of fish in the control group. Weight gain of fish fed to 100% of satiation was significantly (P < 0.05) higher than that of fish fed to 85 and 80% of satiation but not significantly (P > 0.05) different from that of fish fed to 95 and 90% of satiation. Serum total protein, glucose, and glutamic pyruvic transaminase were not significantly (P > 0.05) affected by feeding ratio but triglyceride and glutamic oxaloacetic transaminase were. In considering these results, it can be concluded that optimum daily feeding ratio for growth of subadult olive flounder seemed to be 90% of satiation when fish were fed the EP twice a day during the summer season.  相似文献   

Experimental susceptibility of different life-stages of the giant freshwater prawn, Macrobrachium rosenbergii (de Man), to white spot syndrome virus (WSSV)   总被引：4，自引：1，他引：4  

R B Pramod Kiran  K V Rajendran  S J Jung  & M J Oh 《Journal of fish diseases》2002,25(4):201-207

Studies were conducted by injecting/feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), to different life-stages, namely post-larvae, juveniles, sub-adults and adults of Macrobrachium rosenbergii (de Man). The disease was also induced in brood stock, and the eggs and larvae derived from these animals were subsequently tested for WSSV infection. All the stages except egg used for the experiment were found WSSV positive in histopathology, cross infection bioassay and polymerase chain reaction (PCR) analysis. Experimentally infected post-larvae and juveniles showed a high percentage of mortality and an increased rate of cannibalism. The cumulative mortality in post-larvae was up to 28%; with 28–40% cannibalism resulting in a maximum loss of up to 68%. In juveniles, observed mortality and cannibalism were 10–20% and 6.7–30.0%, respectively, and the maximum loss recorded was 50%. In sub-adults, mortality ranged from 2.8 to 6.7%, cannibalism was up to 20% and the total loss was up to 26.7%. Sub-adults and adults were found to be more tolerant to the infection as evidenced by the mortality pattern. A nested (two-step) PCR resulted in a 570-bp product specific to WSSV in all stages, except the eggs.  相似文献   

Relationship between sea-surface temperature and catch fluctuations in the Pacific stock of walleye pollock in Japan     

Tae-gi?Oh  Kazumi?SakuramotoEmail author  Seizo?Hasegawa  Naoki?Suzuki 《Fisheries Science》2005,71(4):855-861

ABSTRACT:   This paper investigates the relationship between sea-surface temperature (SST) and catch fluctuations in the Pacific stock of walleye pollock Theragra chalcogramma in Japan. Incorporating time lags between years of birth and harvest, the correlation coefficients between the catch and SST in two regions off the east coast of Hokkaido were calculated. The catch in year t had a high negative correlation with the SST during January–April and November–December of the years t- 2 and t- 3 in the spawning area. These results coincided well with the correlation observed in the northern 'Sea of Japan' stock. Both analyses suggested that the long-term catch fluctuations of the two stocks could be explained by the same mechanism, that is, the fluctuations would be explained by the SST in their spawning area during the spawning season using 2–3 or 3–5 years time lags, which corresponded to the dominant age of the catch within these two stocks.  相似文献   

Patient-specific embryonic stem cells derived from human SCNT blastocysts     

Hwang WS  Roh SI  Lee BC  Kang SK  Kwon DK  Kim S  Kim SJ  Park SW  Kwon HS  Lee CK  Lee JB  Kim JM  Ahn C  Paek SH  Chang SS  Koo JJ  Yoon HS  Hwang JH  Hwang YY  Park YS  Oh SK  Kim HS  Park JH  Moon SY  Schatten G 《Science (New York, N.Y.)》2005,308(5729):1777-1783

Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated.  相似文献   

Extent of chromatin spreading determined by roX RNA recruitment of MSL proteins     

Park Y  Kelley RL  Oh H  Kuroda MI  Meller VH 《Science (New York, N.Y.)》2002,298(5598):1620-1623

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Temperature-dependent index of mitotic interval τ0 in greenling Hexagrammos otakii     

In-Seok Park  Eun-Mi Kim  Seon Rang Woo  Sung-Yong Oh  Dong Soo Kim  Jun Wook Hur 《Fisheries Science》2006,72(4):719-722

ABSTRACT:   The aim of this study was to establish effective procedures for chromosome manipulation in greenling Hexagrammos otakii Jordan et Starks, which has enormous aquacultural potential. To accomplish this, temperature-dependent measurements of the mitotic intervals (τ₀) were carried out. The τ₀ in this fish was determined by averaging the duration of the first and third embryonic divisions at temperatures ranging 5–25°C. At higher temperatures, eggs developed faster and underwent more identical development. For greenling, τ₀ were 341.1 ± 3.60 min at 5°C, 275.5 ± 4.53 min at 10°C, 189.7 ± 6.93 min at 15°C, 99.2 ± 8.27 min at 20°C and 34.2 ± 8.74 min at 25°C. There were strong, negative correlations between the τ₀ and water temperatures at all temperatures studied ( Y  = −79.3 X  + 425.3, R ² = 0.9968, where Y is the mitotic interval and X is the temperature).  相似文献   

Sequential sub-passage decreases the differentiation potential of canine adipose-derived mesenchymal stem cells     

Keum Sil Lee  Hye Won Kang  Hoon Taek Lee  Hye-Jin Kim  Chan-Lan Kim  Jae-Young Song  Kyung Woo Lee  Sang-Ho Cha 《Research in veterinary science》2014

Adipose-derived mesenchymal stem cells (AD-MSCs) are abundant in adipose tissue from animals of all ages, are easily isolated, can differentiate into multi-lineage cells, and have a clinical application. This promising potential may only be achieved if the cells are expanding in a large number while maintaining their stemness in sequential passages. In this study, canine AD-MSCs (cAD-MSCs) were individually isolated from five dogs and subjected to proliferative culture with seven sub-passages. The cells at each sub-passage were characterized for properties associated with multipotent MSCs such as proliferation kinetics, expression of MSCs-specific surface markers, expression of molecules associated with self-renewal and differentiation capabilities into mesodermal lineage cells. Proliferation of the cells plateaued at passage 5 by cumulative population doubling level, while cell doubling time gradually increased with passage. MSCs surface markers (CD44, CD90, and CD105) and molecules (Oct 3/4, Sox-2, Nanog and HMGA2) associated with self-renewal were all expressed in the cells between passages 1 to 6 by RT-PCR. In addition, the cells at passage 1, 3 or 6 underwent adipogenic and chondrogenic differentiation under specific induction conditions. However, the level of adipogenic and chondrogenic differentiation was negatively correlated with the number of sub-passage. The present study suggests that sequential sub-passages affect multipotent properties of cAD-MSCs, which should be considered in their therapeutic application in regenerative medicine.  相似文献   

Prediction of compressive strength of cross-laminated timber panel     

Jung-Kwon Oh  Jun-Jae Lee  Jung-Pyo Hong 《Journal of Wood Science》2015,61(1):28-34

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