Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood   总被引：3，自引：0，他引：3  

Alhassan A  Pumidonming W  Okamura M  Hirata H  Battsetseg B  Fujisaki K  Yokoyama N  Igarashi I 《Veterinary parasitology》2005,129(1-2):43-49

With the aim of developing more simple diagnostic alternatives, a differential single-round and multiplex polymerase chain reaction (PCR) method was designed for the simultaneous detection of Babesia caballi and Babesia equi, by targeting 18S ribosomal RNA genes. The multiplex PCR amplified DNA fragments of 540 and 392 bp from B. caballi and B. equi, respectively, in one reaction. The PCR method evaluated on 39 blood samples collected from domestic horses in Mongolia yielded similar results to those obtained from confirmative PCR methods that had been established earlier. Thus, the single-round and multiplex PCR method offers a simple tool for the differential diagnosis of B. caballi and B. equi infections in routine diagnostic laboratory settings as well as in epidemiological studies.  相似文献   

Expression patterns of the implantation-associated genes in the uterus during the estrous cycle in mice     

Lee DS  Yanagimoto Ueta Y  Xuan X  Igarashi I  Fujisaki K  Sugimoto C  Toyoda Y  Suzuki H 《The Journal of reproduction and development》2005,51(6):787-798

The mRNA expression patterns of EGF, HB-EGF, Amphiregulin, EGF receptor, IGF-1, CSF-1, IL-1 alpha, IL-1 beta, IL-1 receptor type 1, IL-1 receptor antagonist, LIF, COX-1, COX-2, Mucin-1, calcitonin, and rat USAG-1 mouse homologue, all of which are involved in the process of conceptus implantation to the endometrium, were examined during the estrous cycle by means of real-time quantitative PCR. COX-2, HB-EGF, LIF, Mucin-1, CSF-1, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist were temporally regulated during the estrous cycle and highly expressed during the estrous stage. In the case of COX-1, EGF, IGF-1, and EGF receptor, the highest mRNA expression was during the diestrous stage. In contrast, the rat USAG-1 mouse homologue mRNA expression did not change during the estrous cycle. These results indicate that rat USAG-1 mouse homologue expression at implantation might be specifically regulated by embryonic factors rather than the maternal environment.  相似文献   

Interploid crossing overcomes plastome-nuclear genome incompatibility in intersubgeneric hybridization between evergreen and deciduous azaleas   总被引：1，自引：0，他引：1  

Kaori Sakai  Yukio Ozaki  Kenji Ureshino  Ikuo Miyajima  Akira Wakana  Hiroshi Okubo 《Scientia Horticulturae》2008

Appearance of albino seedlings caused by plastome-nuclear genome incompatibility was avoided by using polyploid parents in intersubgeneric hybridization between evergreen azaleas and deciduous Rhododendron japonicum f. flavum. Intra- and interploid crosses of 2x or 4x evergreen azaleas with 2xR. japonicum f. flavum showed high capsule set, but their reciprocal crosses failed to set capsules. Green and albino hybrids were obtained from 2x evergreen azaleas × 2xR. japonicum f. flavum crosses where ptDNAs of the green and albino plants were derived from R. japonicum f. flavum (paternal parent) and evergreen azaleas (maternal parents), respectively. All the progenies from the crosses of 4x evergreen azaleas × 2xR. japonicum f. flavum were green triploids with evergreen azalea-specific ptDNA. The efficiency of obtaining green hybrids in interploid crosses was higher than that in intraploid crosses. These results suggest that interploid crosses of 4x evergreen azaleas × 2xR. japonicum f. flavum can overcome plastome-nuclear genome incompatibility between evergreen azalea-specific plastome and the nuclear genome in the resulting hybrid.  相似文献   

Chemical deicer poisoning was suspected as a cause of the 2005-2006 wintertime mortality of small wild birds in Hokkaido     

Tanaka T  Tanoue G  Yamasaki M  Takashima I  Sakoda Y  Ochiai K  Umemura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):607-610

Many small wild birds died in the 2005-2006 wintertime in Hokkaido. Thirteen birds were pathologically examined and it was attempted to detect West Nile and influenza viruses from their organs. Consecutive pathological changes were fresh hemorrhage and acute circulatory failure. Viral detections were negative. Selective occurrence in wintertime, literature review and the results of pathological and virological examinations suggested chemical deicer poisoning as the cause of wild bird death. Chicks treated orally with deicer showed acute death and their pathological changes were similar to those of the wild birds. Because the chicks showed significant elevation of plasma Na concentration, plasma electrolyte analysis of the affected wild birds might be crucial to confirm our tentative diagnosis.  相似文献   

Change of fatty acid composition of the lumbar longissimus during the final stage of fattening in the Japanese Black cattle          下载免费PDF全文

Takeshi Honda  Takafumi Ishida  Ikuo Kobayashi  Yusuke Oguri  Yuto Mizuno  Hideyuki Mannen  Hiroaki Iwaisaki  Shiro Kuge  Kunihiko Saito  Kenji Oyama 《Animal Science Journal》2016,87(4):578-583

Consideration of the shortened fattening period seems to be worthwhile for the realization of profitable beef production. In this study, change of fatty acid composition of the lumbar longissimus during the final stage of fattening was investigated in Japanese Black cattle. Each of 110 fattening animals was sampled three times: the initial two samples were taken by biopsy (25.7 months and 27.5 months on average) and the final one was from carcasses (29.9 months on average). Preliminary analysis indicated that removing muscle tissues from the constant body position of the living animals should be essential for sampling. Average monounsaturated fatty acids (MUFA) at three sampling points were 58.1%, 58.5% and 60.5%, and the differences of the third sampling with the first and second samplings were significant. Both in steers and heifers, MUFA also increased as the fattening stage proceeded, and MUFA of the heifers at all the sampling points were significantly higher than those of the steers. The increasing rate of MUFA rose from 0.21 percentage points (pp)/month at period 1 (from the first sampling to the second sampling) to 0.84 pp/month at period 2 (from the second sampling to the slaughter).  相似文献   

利用聚合酶链式反应技术研究单粒古代稻谷(公元800年)离体DNA片段的扩增     

中村郁郎 《中国水稻科学》1991,5(4):175-179

 在东南亚地区已发掘出很多古代稻谷，如果能从这些古稻谷中把DNA提出加以分析，可以得到有关栽培稻的系统分化和地理传播方面的直接信息。古代稻种的基因型相当复杂，必须要能从单粒种子开始分析，方能得到有价值的资料。为此，我们进行了从单粒古稻谷中提取DNA的研究工作。本研究采用通常提取植物组织中DNA的方法，从在日本挖掘出的古代稻谷单粒种子中提取出了50-100 ng左右的DNA片段。以这些DNA作为模扳，用聚合酶链式反应(Polymerase Chain Reaction，PCR)技术将DNA加以扩增．由其中几个DNA片段初步合成了相当于水稻光敏色素基因的DNA序列。  相似文献   

Molecular detection of Cryptosporidium spp. infections in water buffaloes from northeast Thailand     

Tawin Inpankaew  Tawisa Jiyipong  Kannika Wongpanit  Nongnuch Pinyopanuwat  Wissanuwat Chimnoi  Chanya Kengradomkij  Xuenan Xuan  Ikuo Igarashi  Lihua Xiao  Sathaporn Jittapalapong 《Tropical animal health and production》2014,46(2):487-490

The objectives of this study were to determine the individual and herd-level prevalence and genotype of Cryptosporidium and to identify putative risk factors associated with Cryptosporidium spp. infections in water buffaloes in northeast Thailand. Fecal samples from 600 water buffaloes of 287 farms in six provinces were collected and tested using DMSO-modified acid-fast staining and polymerase chain reaction. The overall prevalence of Cryptosporidium infections in buffaloes was 5.7 and 8.7 % among individual animals and herds, respectively. The provinces with highest infected Cryptosporidium were located in the Sakon Nakhon Basin in the northern part of the region. In addition, higher herd prevalence was observed among farms with more than five buffaloes (30 %) than those with five or less animals (16.2 %). Thirty (88.2 %) of the 34 Cryptosporidium-positive samples were Cryptosporidium parvum and four (11.8 %) were Cryptosporidium ryanae.  相似文献   

Babesia bovis Dihydroorotate Dehydrogenase (BboDHODH) is a Novel Molecular Target of Drug for Bovine Babesiosis     

Ketsarin KAMYINGKIRD  Shinuo CAO  Tatsunori MASATANI  Paul Franck Adjou MOUMOUNI  Patrick VUDRIKO  Ahmed Abd El Moniem MOUSA  Mohamad Alaa TERKAWI  Yoshifumi NISHIKAWA  Ikuo IGARASHI  Xuenan XUAN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):323-330

  相似文献   

Changes in photosynthesis and leaf characteristics with tree height in five dipterocarp species in a tropical rain forest     

Kenzo T  Ichie T  Watanabe Y  Yoneda R  Ninomiya I  Koike T 《Tree physiology》2006,26(7):865-873

Variations in leaf photosynthetic, morphological and biochemical properties with increasing plant height from seedlings to emergent trees were investigated in five dipterocarp species in a Malaysian tropical rain forest. Canopy openness increased significantly with tree height. Photosynthetic properties, such as photosynthetic capacity at light saturation, light compensation point, maximum rate of carboxylation and maximum rate of photosynthetic electron transport, all increased significantly with tree height. Leaf morphological and biochemical traits, such as leaf mass per area, palisade layer thickness, nitrogen concentration per unit area, chlorophyll concentration per unit dry mass and chlorophyll to nitrogen ratio, also changed significantly with tree height. Leaf properties had simple and significant relationships with tree height, with few intra- and interspecies differences. Our results therefore suggest that the photosynthetic capacity of dipterocarp trees depends on tree height, and that the trees adapt to the light environment by adjusting their leaf morphological and biochemical properties. These results should aid in developing models that can accurately estimate carbon dioxide flux and biomass production in tropical rain forests.  相似文献   

Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay     

Boonchit S  Alhassan A  Chan B  Xuan X  Yokoyama N  Ooshiro M  Goff WL  Waghela SD  Wagner G  Igarashi I 《Veterinary parasitology》2006,137(1-2):28-35

Recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Babesia bigemina infection by using a full-length B. bigemina rhoptry-associated protein 1 (rRAP-1) and the truncated C-terminal RAP-1 (rRAP-1/CT). While the rRAP-1 showed cross reactivity between B. bigemina- and Babesia bovis-infected bovine sera, the rRAP-1/CT was highly specific to B. bigemina-infected bovine sera and proved useful in the detection of sequential sera collected from an experimentally infected cow during the acute and latent infection. The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated.  相似文献