Molecular cloning,characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp <Emphasis Type="Italic">Marsupenaeus japonicus</Emphasis>     

Threechada Danwattananusorn  Hidehiro Kondo  Takashi Aoki  Ikuo Hirono 《Fisheries Science》2009,75(5):1231-1238

  相似文献   

Genome analysis of viral hemorrhagic septicemia virus isolated from Japanese flounder Paralichthys olivaceus in Japan     

Ju Jong Byon  Tomokazu Takano  Ikuo Hirono  Takashi Aoki 《Fisheries Science》2006,72(4):906-908

  相似文献   

Effects of arginine supplementation on growth performance and plasma arginine,ornithine and citrulline dynamics of rainbow trout,Oncorhynchus mykiss     

Ichsan Achmad Fauzi  Yutaka Haga  Hidehiro Kondo  Ikuo Hirono  Shuichi Satoh 《Aquaculture Research》2019,50(4):1277-1290

This study aimed to re‐evaluate the effect of excessive arginine supplementation on growth and feed efficiency of rainbow trout, and to assess the effect of dietary arginine supplementation on hepatic amino acid composition, expression of arginase Ⅱ (ARG 2), inducible nitric oxide synthase (iNOS), and heat shock protein 70 (HSP70) genes in the intestine, and plasma arginine, ornithine, citrulline and urea levels at 0, 6, 12 and 18 hr‐postprandial. Rainbow trout (body weight: 60.5–65 g) were fed diets containing 1.47 (control [CTRL]), 3.89 (3.89A) and 5.64% (5.64A) arginine for nine weeks. Higher muscle protein content was observed in 3.89A than CTRL (p < 0.05). In postprandial study, plasma arginine of 5.64A kept increased until 12 hr‐postprandial and reached identical value (around 150 µg/ml) until 18 hr‐postprandial in 5.64A. Significant increase of plasma arginine level was only observed in arginine supplemented group. Meanwhile, plasma citrulline level in CTRL was significantly higher than in 5.64A at 18 hr‐postprandial. A significantly higher hepatic citrulline level was also observed in CTRL than in 5.64A (p < 0.05). Significantly higher plasma and hepatic free ornithine level was observed in 5.64A than CTRL while significantly higher expression of intestinal ARG2 and HSP70 was found in arginine supplemented groups. These results suggest that citrulline availability seems to be stimulated by arginine deficiency in the CTRL and the presence of arginase in the intestine to regulate excess dietary arginine.  相似文献   

Expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines     

Takauji SR  Watanabe M  Uyama R  Nakagawa T  Miyajima N  Mochizuki M  Nishimura R  Sugano S  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):831-834

Protein expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines were examined by western blot analysis and fluorescence immunocytochemistry. A low E-cadherin expression was observed in FNN-m cells. Furthermore, compared to other cell lines, two E-cadherin bands existed in FMC-p1 cells and were localized in the perinuclear region; distinct radial lines were observed in the cytoplasm. A low alpha-catenin expression was observed in FON-m cells, but there were no apparent abnormalities in its localization. In contrast, similar levels of beta-catenin expression and cytoplasmic localization were observed in all cell lines.  相似文献   

Cytotoxicity of Streptococcus agalactiae secretory protein on tilapia cultured cells     

Iyapa Palang  Ikuo Hirono  Saengchan Senapin  Wanna Sirimanapong  Boonsirm Withyachumnarnkul  Rapeepun Vanichviriyakit 《Journal of fish diseases》2020,43(10):1229-1236

Streptococcus agalactiae secrete virulence factors believed to be able of killing host tissues, especially under elevated water temperature. A direct effect of S. agalactiae secretory products on tilapia cells was tested on the tilapia kidney (TK-1) cell culture. The bacteria were cultured under four different temperature levels: 22, 29, 32 and 37°C; the cell-free portion was processed through SDS-PAGE; and distinct bands were identified by LC-MS/MS. At least, three virulence factors were identified, Bsp, PcsB and CAMP factor, with increasing levels as the cultured temperature rose. Expressions of bsp, pcsB and cfb were also up-regulated with the rising of the temperature in S. agalactiae culture. The supernatant from the bacteria cultured under specified temperatures was added into TK-1 cell-cultured wells. Morphological damage and mortality of the cultured cells, as determined by MTT method, were increased progressively from the supernatant treatment according to the rise of temperature in S. agalactiae culture. This study suggests that the production of the three virulence factors of S. agalactiae reported herein is temperature-dependent, and it is likely that CAMP factor directly kills the TK-1 cells since the other two types of protein are involved in S. agalactiae cell division and the bacterial adherence to host tissues.  相似文献   

Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds     

Saiko Sugawara  Toshihiko Ito  Sho Sato  Mari Yokoo  Yuki Mori  Kano Kasuga  Ikuo Kojima  Tomokazu Fukuda  Ken‐ichi Yamanaka  Miki Sakatani  Masashi Takahashi  Masayuki Kobayashi 《Animal Science Journal》2013,84(3):275-280

Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the proper development of bovine embryos. We recently determined the FGF4 gene sequence in eight cattle derived from three breeds and revealed a common nucleotide sequence of the structural gene encoding FGF4, which leads to the deletion and mutation of amino acid sequences in the mature FGF4 (Pro³²‐Leu²⁰⁶) compared with the sequence previously reported. In the present study, HisbFGF4, a 6× histidine‐tagged bovine FGF4 (Pro³²‐Leu²⁰⁶), was produced in Escherichia coli based on the validated nucleotide sequence and purified by heparin column chromatography. In primary bovine fibroblasts, HisbFGF4 showed significant mitogenic activity, whereas, intriguingly, the activity of a commercially available recombinant human FGF4 (Gly²⁵‐Leu²⁰⁶) produced in E. coli was weaker than that of HisbFGF4. In conclusion, the present study provides a simple method for the production of a bioactive bovine FGF4 derivative in E. coli utilizing its structural gene elucidated by us.  相似文献   

Cloning of an interleukin-3 receptor gene: a member of a distinct receptor gene family   总被引：71，自引：0，他引：71  

N Itoh  S Yonehara  J Schreurs  D M Gorman  K Maruyama  A Ishii  I Yahara  K Arai  A Miyajima 《Science (New York, N.Y.)》1990,247(4940):324-327

Interleukin-3 (IL-3) binds to its receptor with high and low affinities, induces tyrosine phosphorylation, and promotes the proliferation and differentiation of hematopoietic cells. A binding component of the IL-3 receptor was cloned. Fibroblasts transfected with the complementary DNA bound IL-3 with a low affinity [dissociation constant (Kd) of 17.9 +/- 3.6 nM]. No consensus sequence for a tyrosine kinase was present in the cytoplasmic domain. Thus, additional components are required for a functional high affinity IL-3 receptor. A sequence comparison of the IL-3 receptor with other cytokine receptors (erythropoietin, IL-4, IL-6, and the beta chain IL-2 receptor) revealed a common motif of a distinct receptor gene family.  相似文献   

Retention of Rice dwarf virus by Descendants of Pairs of Viruliferous Vector Insects After Rearing for 6 Years     

Honda K  Wei T  Hagiwara K  Higashi T  Kimura I  Akutsu K  Omura T 《Phytopathology》2007,97(6):712-716

ABSTRACT Rice dwarf virus (RDV) is characterized by its unusual ability to multiply in both plants and leafhopper vector insects and by its transovarial mode of transmission. Colonies of Nephotettix cincticeps, derived originally from pairs of leafhoppers infected with an ordinary strain of RDV, were maintained for 6 years in the laboratory and were found, at the end of this time, still to harbor RDV. Moreover, the isolate of RDV, designated RDV-I, obtained from these colonies retained the ability to infect rice plants. When we raised leafhoppers separately from eggs that had been placed individually on pieces of water-soaked filter paper and reared them in the presence of healthy rice seedlings, we found that all of these leafhoppers harbored RDV. This observation suggested that RDV-I had been maintained in the leafhoppers by transovarial transmission. Two further observations, namely, the low rate of acquisition of RDV by virus-free insect nymphs on symptomless plants on which viruliferous insects had been reared, and the fact that only 2 to 5% of plants had symptoms when rice seedlings were inoculated via RDV-I-viruliferous insects, confirmed that the maintenance of RDV-I by any other mode of transmission through plants and insects was unlikely. This efficient and long-term maintenance of RDV in a population of viruliferous insects might explain the prolonged duration of rice dwarf disease in the field, once there has been a serious outbreak.  相似文献   

Suppressive effect of ATP on the denaturation of sarcoplasmic reticulum (Ca2+)-ATPase from southern bluefin tuna Thunnus maccoyii and its biochemical properties     

Chunhong Yuan  Yuli Takeda  Wakana Nishida  Ikuo Kimura 《Fisheries Science》2016,82(1):147-153

  相似文献   

Mycoplasma ovis detected in free-living Japanese serows, Capricornis crispus     

Ohtake Y  Nishizawa I  Sato M  Watanabe Y  Nishimura T  Matsubara K  Nagai K  Harasawa R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):371-373

Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18 °C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. This was the first demonstration of hemotropic mycoplasma infections among the free-living Japanese serows in Japan.  相似文献