Effects of Shifting Cultivation on Soil Ecosystems in Sarawak, Malaysia IV. Chemical Properties of the Soils and Runoff Water at Niah and Bakam Experimental Sites     

Sota Tanaka  Joseph Jawa Kendawang  Nanae Yoshida  Kenji Shibata  Augustine Jee  Kenzo Tanaka  Ikuo Ninomiya  Katsutoshi Sakurai 《Soil Science and Plant Nutrition》2005,51(4):525-533

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Expression analysis of defense-related genes in wild kiwifruit (Actinidia rufa) tolerant to bacterial canker     

Kaji  Ryoma  Yariuchi  Rina  Fujii  Yumi  Taniguchi  Shiduku  Uji  Yuya  Suzuki  Go  Kashihara  Keita  Kisaki  Gan  Suezawa  Katsuhiko  Ohtani  Mamoru  Kataoka  Ikuo  Akimitsu  Kazuya  Gomi  Kenji 《Journal of General Plant Pathology》2021,87(6):361-365

Journal of General Plant Pathology - Kiwifruit bacterial canker, which is caused by Pseudomonas syringae pv. actinidiae biovar 3 (Psa3), is found throughout kiwifruit-growing countries. Here, we...  相似文献   

Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens   总被引：1，自引：0，他引：1  

Xuan X  Chahan B  Huang X  Yokoyama N  Makala LH  Igarashi I  Fujisaki K  Maruyama S  Sakai T  Mikami T 《Veterinary parasitology》2002,108(2):179-182

The prevalence of equine piroplasmosis in Xinjiang province, China, was examined by enzyme-linked immunosorbent assays (ELISAs). A total of 70 serum samples were taken from horses pastured on three farms in western Xinjiang, and examined for diagnosis of equine Babesia equi (B. equi) infection and B. caballi infection by ELISAs using recombinant equi merozoite antigen 1 (EMA-1) and recombinant P48 antigen, respectively. Of the 70 samples, 28 (40.0%) and 17 (24.3%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 11 (15.7%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in western Xinjiang. To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China.  相似文献   

Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting     

Akiba M  Uchida I  Nishimori K  Tanaka K  Anzai T  Kuwamoto Y  Wada R  Ohya T  Ito H 《Veterinary microbiology》2003,92(4):379-388

Equine paratyphoid is caused by Salmonella enterica serovar Abortusequi, and manifests mainly as abortion in the mare. We compared S. Abortusequi strains isolated in Japan and other countries using pulsed-field gel electrophoresis (PFGE) and fluorescent amplified-fragment length polymorphism (FAFLP) analysis. PFGE analysis of S. Abortusequi strains gave 21-27 fragments ranging in size from 33 to 602kb. Although two PFGE profiles were observed among the 20 S. Abortusequi isolates in Japan, the restriction fragments originating from the chromosome were common between the two profiles. The similarity index of the two profiles was 90.9%, while those between Japanese and five other S. Abortusequi strains were 29.8-37.5%. On the other hand, FAFLP analysis of S. Abortusequi strains generated 64-67 amplified fragments ranging in size from 100 to 400bp. One polymorphic fragment was observed among the 20 S. Abortusequi isolates in Japan. These data indicate the close relation of this agent in Japan. S. Abortusequi strains sharing a common ancestry might have been conserved in Japan.  相似文献   

The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among Babesia bovis Detected from Cattle Populations in Thailand,Brazil and Ghana     

Daisuke NAGANO  Thillaiampalam SIVAKUMAR  Alane Caine Costa DE DE MACEDO  Tawin INPANKAEW  Andy ALHASSAN  Ikuo IGARASHI  Naoaki YOKOYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1463-1470

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A population genetics-based approach reveals candidate gene regions important in the determination of resistance specificity for the Pik family of rice blast resistance genes     

Ikuo Ashikawa  Ryuji Ishikawa 《Journal of General Plant Pathology》2013,79(3):178-181

Allelic resistance (R) genes at the Pik locus for rice blast resistance consist of two genes, designated as the first gene and the second gene, which each have a nucleotide-binding site and a leucine-rich repeat. We investigated allelic nucleotide sequence diversity at this locus in 36 rice lines and searched for gene regions showing high rates of nonsynonymous nucleotide substitutions. We detected such regions within and near the coiled-coil domain of the first gene but detected only a few nucleotide substitutions in the second gene, suggesting the importance of the N-terminal part of the first protein in the determination of R specificity.  相似文献   

Suppressive effect of ATP on autoxidation of tuna oxymyoglobin to metmyoglobin     

Kota Inohara  Ikuo Kimura  Chunhong Yuan 《Fisheries Science》2013,79(3):503-511

The discoloration of tuna meat proceeds during frozen storage at around ?20 °C. On the other hand, the discoloration of highly fresh tuna meat could effectively be suppressed even if stored at ?20 °C. However, the suppressive mechanism of the discoloration is not well understood. Here the effects of ATP on the autoxidation rate and molecular structure of tuna myoglobin are reported. The autoxidation rate of southern bluefin tuna Thunnus maccoyii myoglobin at 25 °C was suppressed in the presence of ATP especially in acidic pH range. Mixing ATP with myoglobin induced a spectral perturbation in the soret region of myoglobin. This spectral perturbation was observed as a function of the ATP concentration. Quenching of myoglobin fluorescence was also caused by ATP, saturating at around 0.5 mM ATP. According to dynamic light-scattering measurements, the molecular weights of tuna Mb changed from 15.5 to 11.3 kDa with ATP and zeta-potential measurements gave also a negative surface charge without ATP and a positive one with ATP, respectively. The above results indicate that ATP-induces changes in the conformational structure of myoglobin. The effects of ATP on myoglobin could thus provide a possible mechanism to regulate the autoxidation of myoglobin.  相似文献   

Effects of feed restriction on the expression profiles of the glucose and fatty acid metabolism-related genes in rainbow trout Oncorhynchus mykiss muscle     

Hidehiro Kondo  Satomitsu Suda  Yuriko Kawana  Ikuo Hirono  Reiko Nagasaka  Gen Kaneko  Hideki Ushio  Shugo Watabe 《Fisheries Science》2012,78(6):1205-1211

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章古台地区樟子松年轮宽度和管胞长度的径向变异     

齐锦秋  郝建锋  吴忠  古川郁夫 《辽宁林业科技》2009,(1):14-17

对章古台地区不同密度樟子人工林松年轮宽度和管胞长度进行测定,结果表明：章古台地区胸径年轮数30a的樟子松林,已出现生长衰退现象,生长衰退期开始林龄与林分密度有关,降低林分密度可延缓衰退期出现林龄。樟子松管胞长度与距离髓心的年轮数之间存在Y=alnx＋b（Y：管胞长度;X：距离髓心的年轮数．b：第1年轮的管胞长度）关系。不同密度樟子松年轮宽度不同,但管胞长度与未成熟材范围无显著变化,未成熟材范围距离髓心的年轮数均为21～23a。  相似文献   

Cryopreservation of male and female gonial cells by vitrification in the critically endangered cyprinid honmoroko <Emphasis Type="Italic">Gnathopogon caerulescens</Emphasis>     

Shogo Higaki  Takaaki Todo  Reiko Teshima  Ikuo Tooyama  Yasuhiro Fujioka  Noriyoshi Sakai  Tatsuyuki Takada 《Fish physiology and biochemistry》2018,44(2):503-513

We investigated the feasibility of cryopreservation of spermatogonia and oogonia in the critically endangered cyprinid honmoroko Gnathopogon caerulescens using slow-cooling (freezing) and rapid-cooling (vitrification) methods. Initially, we examined the testicular cell toxicities and glass-forming properties of the five cryoprotectants: ethylene glycol (EG), glycerol (GC), dimethyl sulfoxide (DMSO), propylene glycol (PG), and 1,3-butylene glycol (BG), and we determined cryoprotectant concentrations that are suitable for freezing and vitrification solutions, respectively. Subsequently, we prepared the freezing solutions of EG, GC, DMSO, PG, and BG at 3, 2, 3, 2, and 2 M and vitrification solutions at 7, 6, 5, 5, and 4 M, respectively. Following the cryopreservation of the testicular cells mainly containing early-stage spermatogenic cells (e.g., spermatogonia and primary spermatocytes), cells were cultured for 7 days and immunochemically stained against germ cell marker protein Vasa. Areas occupied by Vasa-positive cells indicated that vitrification led to better survival of germ cells than the freezing method, and the best result was obtained with 5 M PG, about 50% recovery of germ cells following vitrification. In the case of ovarian cells containing oogonia and stage I, II, and IIIa oocytes, vitrification with 5 M DMSO resulted the best survival of oogonia, with equivalent cell numbers to those cultured without vitrification. The present data suggest that male and female gonial cells of the endangered species G. caerulescens can be efficiently cryopreserved using suitable cryoprotectants for spermatogonia and oogonia, respectively.  相似文献