全文获取类型
收费全文 | 71291篇 |
免费 | 2805篇 |
国内免费 | 33篇 |
专业分类
林业 | 6533篇 |
农学 | 3038篇 |
基础科学 | 392篇 |
9919篇 | |
综合类 | 11765篇 |
农作物 | 4230篇 |
水产渔业 | 4268篇 |
畜牧兽医 | 27054篇 |
园艺 | 1659篇 |
植物保护 | 5271篇 |
出版年
2019年 | 463篇 |
2018年 | 3792篇 |
2017年 | 3852篇 |
2016年 | 1989篇 |
2015年 | 732篇 |
2014年 | 790篇 |
2013年 | 1794篇 |
2012年 | 2416篇 |
2011年 | 4180篇 |
2010年 | 3656篇 |
2009年 | 2949篇 |
2008年 | 3335篇 |
2007年 | 3829篇 |
2006年 | 1594篇 |
2005年 | 1621篇 |
2004年 | 1514篇 |
2003年 | 1578篇 |
2002年 | 1397篇 |
2001年 | 1547篇 |
2000年 | 1543篇 |
1999年 | 1229篇 |
1998年 | 459篇 |
1997年 | 479篇 |
1996年 | 475篇 |
1995年 | 531篇 |
1994年 | 476篇 |
1993年 | 507篇 |
1992年 | 961篇 |
1991年 | 924篇 |
1990年 | 914篇 |
1989年 | 938篇 |
1988年 | 888篇 |
1987年 | 902篇 |
1986年 | 894篇 |
1985年 | 901篇 |
1984年 | 727篇 |
1983年 | 659篇 |
1979年 | 651篇 |
1978年 | 545篇 |
1977年 | 477篇 |
1976年 | 464篇 |
1975年 | 507篇 |
1974年 | 693篇 |
1973年 | 608篇 |
1972年 | 680篇 |
1971年 | 672篇 |
1970年 | 599篇 |
1969年 | 600篇 |
1968年 | 468篇 |
1967年 | 523篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
Waldrop JE Pike FS Dulisch ML Ortega TM Gliatto JM 《Journal of the American Animal Hospital Association》2001,37(1):81-85
Lymphangiosarcoma of the pulmonary pleura was found to be the cause of persistent chylothorax in an eight-year-old, intact male golden retriever. After a two-month course of medical management, a lymphangiogram and thoracic duct ligation were performed with the objective of decreasing further effusion. At surgery, gross lung pathology was biopsied and yielded a histopathological diagnosis of pulmonary pleural lymphangiosarcoma. A relatively rare tumor, lymphangiosarcoma of the pulmonary pleura has not previously been documented as a source of chylothorax in the dog. 相似文献
992.
Plain spinal radiography was performed in 40 dachshunds at regular intervals from 6 or 12 months of age to 2 years of age. A follow-up study at 3 to 4 years of age included 12 dogs. High incidence rates of intervertebral disk calcification were seen at 6 to 18 months of age. The number of dogs affected and number of calcified disks seemed to reach a steady level or a maximum at about 24 to 27 months of age. Dissolution of previously calcified disks without clinical signs was demonstrated, causing decreasing numbers of visibly calcified disks after 2 years of age. Radiographic examination for calcified intervertebral disks in the dachshund is recommended at 24 to 30 months of age for heritability studies and selective breeding. 相似文献
993.
994.
995.
Relationship between serum TNF activity and insulin resistance in dairy cows affected with naturally occurring fatty liver 总被引:2,自引:0,他引:2
Ohtsuka H Koiwa M Hatsugaya A Kudo K Hoshi F Itoh N Yokota H Okada H Kawamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1021-1025
To clarity the relationship between tumor necrosis factor (TNF) and insulin resistance in dairy cows affected with fatty liver, naturally occurring cases were investigated. The affected cows were classified into following three groups according to histopathologic findings of the liver: mild fat droplet deposition (group 1; n=11), severe fat droplet deposition (group 2; n=10), and cloudy swelling (group 3; n=8). Serum TNF activities in Group 2 (8.67 +/- 2.16 U/ml) and Group 3 (11.65 +/- 1.92 U/ml) were significantly higher than that in Group 1 (3.57 +/- 0.81 U/ml) (p<0.05). The insulin-tolerance tests showed that the insulin-stimulated glucose disposal rates (GDR) in Group 2 (27.6 +/- 7.8%) and Group 3 (15.8 +/- 9.1%) were significantly lower than that in Group 1 (41.7 +/- 9.8%). There was a significant negative correlation between serum TNF activity and GDR in affected cows (r=-0.56, p<0.01). These results indicate that serum TNF activity is correlated with insulin resistance in cows with fatty liver. 相似文献
996.
997.
Núñez JI Martín MJ Piccone ME Carrillo E Palma EL Dopazo J Sobrino F 《Veterinary research》2001,32(1):31-45
An analysis of the informative content of sequence stretches on the foot-and-mouth disease virus (FMDV) VPI gene was applied to two important viral serotypes: A and O. Several sequence regions were identified to allow the reconstruction of phylogenetic trees equivalent to those derived from the whole VPI gene. The optimal informative regions for sequence windows of 150 to 250 nt were predicted between positions 250 and 550 of the gene. The sequences spanning the 250 nt of the 3' end (positions 400 to 650), extensively used for FMDV phylogenetic analyses, showed a lower informative content. In spite of this, the use of sequences from this region allowed the derivation of phylogenetic trees for type A and type O FMDVs which showed topologies similar to those previously reported for the whole VP1 gene. When the sequences determined for viruses isolated in Argentina, between 1990 and 1993, were included in these analyses, the results obtained revealed features of the circulation of type A and type O viruses in the field, in the months that preceded the eradication of the disease in this country. Type A viruses were closely related to an Argentinean vaccine strain, and defined an independent cluster within this serotype. Among the type O viruses analysed, two groups were distinguished; one was closely related to the South American vaccine strains, while the other was grouped with viruses of the O3 subtype. In addition, a detailed phylogeny for type A FMDV is presented. 相似文献
998.
Van Overbeke I Chiers K Ducatelle R Haesebrouck F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(1):15-20
The efficacy of a subunit vaccine containing the Apx toxins of Actinobacillus pleuropneumoniae and transferrin-binding proteins was determined. Ten pigs were vaccinated twice with the vaccine. Eight control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 10(6.5) colony-forming units (CFU) of an A. pleuropneumoniae serotype 9 strain. In the vaccine group, none of the pigs died after inoculation. Only one pig of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The mean percentage of affected lung tissue was 64% in the control group and 17% in the vaccine group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 5.0 x 10(8) CFU/g in the control group and 3.0 x 10(6) CFU/g in the vaccine group. It was concluded that the vaccine induced partial protection against severe challenge. 相似文献
999.
Contamination of pigs by nose-to-nose contact or airborne transmission of Salmonella Typhimurium. 总被引:2,自引:0,他引:2
K Proux R Cariolet P Fravalo C Houdayer A Keranflech F Madec 《Veterinary research》2001,32(6):591-600
The aim of this study is to assess the risk of contamination by Salmonella Typhimurium of pigs by nose-to-nose contact or the airborne route. Thirty twelve-week-old SPF pigs were divided into 4 groups housed in 4 different rooms: the first room contained Salmonella-free control pigs (n = 4), the second room had 10(3) CFU S. Typhimurium inoculated pigs (n = 5) and non-inoculated "contact" pigs (n = 4), the third room had pigs (n = 8) receiving potentially contaminated air from the following room through a hole (4 pigs housed in the pen situated near the hole and 4 pigs in the pen at the opposite side of the room), and the fourth room had pigs (n = 5) inoculated with 10(6) CFU Salmonella Typhimurium and also non inoculated "contact" pigs (n = 4). The "contact" and the inoculated pigs were housed in adjacent pens allowing nose-to-nose contact. The 5 pigs orally inoculated with 10(6) CFU S. Typhimurium were bacteriologically and serologically positive 1 week later and their environment was contaminated as early as 1 day pi. The faecal samples of 4 nose-to-nose contact pigs were bacteriologically positive and one of them was seropositive 5 weeks pi before the pigs were commingled. The 8 pigs housed in the third room received S. Typhimurium by an active airflow coming from the contaminated room (1000 m3/hour). Their faecal samples remained negative until 8 weeks pi but the environmental swabs taken in the room close to the airinlet were contaminated 2 days pi and positive swabs were found elsewhere in the room 5 weeks pi. Two seropositive pigs were encountered 8 weeks pi in the pen situated near the hole. Only one among the 5 pigs inoculated with 10(3) CFU had bacteriologically positive faeces 1-week pi and the 4 pigs kept in nose-to-nose contact with them remained negative. A dose of 10(3) CFU was too small to induce persistent excretion and to stimulate a humoral immune response. However, the dose of 10(6) CFU induced contamination of nose-to-nose contact pigs and contamination of the environment by airflow. 相似文献