High throughput generation of promoter reporter (GFP) transgenic lines of low expressing genes in Arabidopsis and analysis of their expression patterns     

Yong-Li Xiao  Julia C Redman  Erin L Monaghan  Jun Zhuang  Beverly A Underwood  William A Moskal  Wei Wang  Hank C Wu  Christopher D Town 《Plant methods》2010,6(1):18

Background  

Although the complete genome sequence and annotation of Arabidopsis were released at the end of year 2000, it is still a great challenge to understand the function of each gene in the Arabidopsis genome. One way to understand the function of genes on a genome-wide scale is expression profiling by microarrays. However, the expression level of many genes in Arabidopsis genome cannot be detected by microarray experiments. In addition, there are many more novel genes that have been discovered by experiments or predicted by new gene prediction programs. Another way to understand the function of individual genes is to investigate their in vivo expression patterns by reporter constructs in transgenic plants which can provide basic information on the patterns of gene expression.  相似文献   

Myeloma associated with immunoglobulin lambda-light chain derived amyloid in a dog     

O Geisel  M Stiglmair-Herb  R P Linke 《Veterinary pathology》1990,27(5):374-376

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A streamlined method for systematic,high resolution <Emphasis Type="Italic">in situ</Emphasis> analysis of mRNA distribution in plants     

Drea S  Corsar J  Crawford B  Shaw P  Dolan L  Doonan JH 《Plant methods》2005,1(1):8

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Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers     

Shaw SC  Wood JL  Freeman J  Littlewood JD  Hannant D 《American journal of veterinary research》2004,65(7):1014-1020

OBJECTIVE: To estimate the heritability of atopic dermatitis in Golden and Labrador Retrievers. ANIMALS: 429 dogs related to 13 dogs with atopic dermatitis. PROCEDURE: Atopic dermatitis was defined on the basis of the type and frequency of clinical signs recorded in the clinical records, and each dog was classified with atopic dermatitis or probable atopic dermatitis or as nonatopic. By use of data from atopic and nonatopic dogs, regression analyses of parental status on offspring status were performed to estimate heritability. RESULTS: There was no difference in the frequency of atopic dermatitis between sexes or between breeds. There was a marked association between the atopic status of the parent and that of the offspring, particularly for sires. By use of data from 32 litters in which the status of both parents was known and considering only those dogs classified with atopic dermatitis or as nonatopic, the heritability (+/- SE) of atopic dermatitis was estimated to be 0.47 (+/- 0.17). CONCLUSIONS AND CLINICAL RELEVANCE: Atopic dermatitis has a strong genetic component, and breeding of dogs with clinical signs of atopic dermatitis should be discouraged.  相似文献   

Training in client relations and communication skills in veterinary medical curricula and usage after graduation     

Kogan LR  Butler CL  Lagoni LK  Brannan JK  McConnell SM  Harvey AM 《Journal of the American Veterinary Medical Association》2004,224(4):504-507

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Clinical comparison of isoflurane and sevoflurane anaesthesia in the gerbil (Meriones unguiculatus)     

Henke J  Strack T  Erhardt W 《Berliner und Münchener tier?rztliche Wochenschrift》2004,117(7-8):296-303

The objective of this study was a comparison of the volatile anaesthetics isoflurane and sevoflurane in terms of their clinical effects in gerbils (Meriones unguiculatus) (n=12 each). Induction of anaesthesia was performed in a body chamber with an anaesthetic concentration of 4.0 Vol.% at an oxygen flow of 500 ml/min for isoflurane and 8.0 Vol.% at an oxygen flow of 1000 ml/min for sevoflurane, respectively. Anaesthesia was maintained via nose cone with an anaesthetic concentration of 2.8 to 3.2 Vol.% at an oxygen flow of 200 ml/min for isoflurane and 5.0 to 5.2 Vol.% at an oxygen flow of 400 ml/min for sevoflurane. Those anaesthetic concentrations ensured reflex status conform with surgical tolerance. In spite of its higher blood-gas coefficient induction time was slightly faster for isoflurane. Recovery time was significantly longer in the isoflurane group than it was in the sevoflurane group. Both inhalants caused respiratory depression. Respiratory rate was lower in sevoflurane animals compared to isoflurane. The animals were positioned on a heating pad immediately after induction, thus a decrease of the body temperature could be prevented. Both inhalants can be recommended for usage in gerbils. Sevoflurane showed no clinical benefit compared to isoflurane.  相似文献   

Anion gap correlates with serum D- and DL-lactate concentration in diarrheic neonatal calves     

Ewaschuk JB  Naylor JM  Zello GA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):940-942

The objective of this study was to investigate the relationship between serum D- and L-lactate concentrations, and anion gap (AG) in neonatal calves. The association of AG with lactic acidosis in diarrheic calves has only been investigated by measurement of L-lactate in calves with experimentally induced diarrhea. D-lactate has recently been reported to be present in high concentrations in the serum of some diarrheic neonatal calves. The contribution of this acid to AG is not reported. The relationship between AG and L- and D-lactate concentrations was examined in 24 healthy calves and 52 calves with naturally occurring infectious diarrhea with metabolic acidosis. AG was calculated as [Na+ + K+] - [Cl- + HCO3-]. D- and L-lactate were quantified using high-performance liquid chromatography. There was no correlation between L-lactate and AG, contrary to previous reports in the literature. Moderate correlations between D-lactate concentration and AG (r = .74, P < .0001), and between DL-lactate and AG (r = .77), P < .0001) were detected. No differences existed due to the age or sex of the calf. This study indicates that AG provides information on the nature of acidosis in the diarrheic, neonatal calf and reinforces the importance of investigating clinical, in addition to experimental, populations.  相似文献   

Gene expression changes in MDBK cells infected with genotype 2 bovine viral diarrhoea virus     

Neill JD  Ridpath JF 《Veterinary microbiology》2003,96(4):301-312

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Certificates in general practice     

Manning P  Viner B  Shuttleworth S  Whipp C  Muckle A  Posnett S  Duncanson G  Boness J 《The Veterinary record》2003,152(23):723

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Role of nonsteroidal anti-inflammatory drugs in gastrointestinal tract injury and repair     

Tomlinson J  Blikslager A 《Journal of the American Veterinary Medical Association》2003,222(7):946-951

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