Increased humoral antibody response of foot-and-mouth disease virus vaccine in growing pigs pre-treated with poly-γ-glutamic acid     

Jee-Hoon Lee  Ik-Jae Kang  A-Reum Kim  You-Sun Noh  Hee-Chun Chung  Bong-Kyun Park 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):253-256

This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic acid (γ-PGA) three days before vaccination. Antibody against FMD virus serotype O was measured 0, 2, 4 and 6 weeks post-vaccination, using a PrioCHECK FMDV type O ELISA kit. The results showed that positive antibody reactions against FMDV serotype O antigen among a component of the vaccine significantly increased in response to pre-injection with γ-PGA.  相似文献   

Rumen adaptation of swamp buffaloes (Bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet     

Metha Wanapat  Kampanat Phesatcha  Sungchhang Kang 《Tropical animal health and production》2016,48(6):1135-1140

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Comparison of two types of CIDR-based timed artificial insemination protocols for repeat breeder dairy cows     

Kim UH  Suh GH  Hur TY  Kang SJ  Kang HG  Park SB  Kim HS  Kim IH 《The Journal of reproduction and development》2007,53(3):639-645

This study compared two types of controlled internal drug release (CIDR)-based timed artificial insemination (TAI) protocol for treatment of repeat breeder dairy cows. In the first trial of the experiment, 55 repeat breeder cows were randomly assigned to the following two treatments. (1) In the EB group, a CIDR device was inserted into the cows, and then the cows were administered an injection of 1 mg estradiol benzoate (EB) plus 50 mg progesterone (P4; Day 0). On Day 7, they were given an injection of PGF(2alpha) and the CIDR device was removed. The cows were given an injection of 1 mg EB on Day 8 and were subjected to TAI 30 h later (n=27). (2) In the gonadotrophin releasing hormone (GnRH) group, a CIDR device was inserted into the cows, and then the cows were administered an injection of 250 microg gonadorelin (GnRH; Day 0). On Day 7, they were given an injection of PGF(2alpha) and the CIDR device was removed. The cows were given an injection of 250 microg GnRH on Day 9 and were subjected to TAI 17 h later (n=28). In the second trial, 41 repeat breeder cows that were confirmed as not pregnant in the first trial were randomly assigned to the same two treatments used in the first trial (an EB group of 20 cows and a GnRH group of 21 cows). The ovaries of 15 cows from each group were examined by transrectal ultrasonography in order to observe the changes in ovarian structures, and blood samples were collected for analysis of serum P4 concentrations. The pregnancy rates following TAI in the first (18.5 vs. 32.1%) and second (40.0 vs. 38.1%) trials and the combined rates (27.7 vs. 34.7%) did not differ between the EB and GnRH groups. The proportions of cows with follicular wave emergence within 7 days did not differ between the EB (12/15) and GnRH groups (13/15). The interval to wave emergence was shorter (P<0.01) in the GnRH group than in the EB group, but there was no difference in the mean diameters of dominant follicles on Day 7 between the groups. Moreover, the proportions of cows with synchronized ovulation following a second EB or GnRH treatment did not differ between the groups. In conclusion, treatment with either EB or GnRH in a CIDR-based TAI protocol results in synchronous follicular wave emergence, follicular development, synchronous ovulation, and similar pregnancy rates for TAI in repeat breeder cows.  相似文献   

Therapeutic trial of granulocyte-colony stimulating factor for dilated cardiomyopathy in three dogs     

Park C  Yoo JH  Jeon HW  Kang BT  Kim JH  Jung DI  Lim CY  Lee HJ  Hahm DH  Woo EJ  Park HM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):951-955

Three dogs were presented to us for evaluation of cardiac problems. Electrocardiographic recordings revealed severe tachyarrhythmia and atrial fibrillation with ventricular tachycardia in 2 of the 3 dogs. The echocardiographic findings of the 3 dogs revealed markedly decreased fractional shortening and a marked increase in E-point septal separation. Based on the results of electrocardiographic and echocardiographic evaluation, the 3 dogs were diagnosed as dilated cardiomyopathy (DCM). The dogs were treated with conventional cardiac medication, but cardiac function did not improve and the clinical signs remained. We subsequently attempted treatment with granulocyte-colony stimulating factor (G-CSF; 10 microg/kg, subcutaneously). The specific purpose of G-CSF therapy for DCM was to improve cardiac function and a significant improvement in cardiac function was confirmed. The three dogs had no treatment side effects. This case report suggests that G-CSF might have therapeutic effects for medically refractory DCM in dogs.  相似文献   

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages   总被引：3，自引：0，他引：3  

Kim MK  Hossein MS  Oh HJ  Fibrianto HY  Jang G  Kim HJ  Hong SG  Park JE  Kang SK  Lee BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):627-632

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.  相似文献   

Calcium carbonate-modified plant sporopollen capsule as an eco-friendly microvehicle for controlled release of pesticide     

Sheng Xiang  Huajun Kang  Ali Chai  Yanxia Shi  Xuewen Xie  Lei Li  Tengfei Fan  Baoju Li 《Pest management science》2023,79(4):1604-1614

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Atypical antibiotic-responsive Staphylococcus pseudintermedius-associated tumor-like meibomitis in the upper eyelid: A case report of two dogs     

Sol Kim  Yeseul Yang  Su An Kim  SeungJun Lee  Yongbaek Kim  Kangmoon Seo  Seonmi Kang 《Veterinary ophthalmology》2023,26(5):458-463

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EXPERIMENTAL INVESTIGATION ON VIBRATION AND NOISE BY C6132 LATHE     

Wang Yanchun Ding Kang Xie MingHuang Qiang Yi Zhulin 《保鲜与加工》1992,(5):1-7

This paper presents the principle of high-pass-absolute-value demodulation tcchnigue which is applied with spectrum analysis to investigate the vibration and noise produced by the C61 32 Lathe. The main noise sourse is found out and some reliable data are provided to decrease the noise and vibration and to improve the dynamic performence of the c6132 Lathe.  相似文献   

单株幼龄苹果树修剪仿真研究     

杨丽丽  陈甲风  谢锐  华净  康孟珍  董乔雪 《农业机械学报》2015,46(S1):41-44

修剪是果树生产中重要的技术手段,要想研究更加合理的修剪技术体系,就必须保证能够对修剪反应有准确、直观和及时的判断,而仿真模拟技术提供了这种可能。以幼龄苹果树为研究对象,采用不同修剪处理对比的方法,在田间试验的基础上,总结修剪反应规律,构建苹果树结构和修剪模型。在此基础上基于Qt框架和OpenGL图形库,开发了单株苹果树修剪仿真软件平台。结合优化算法,通过仿真平台模拟了给定生产目标的修剪方案优化,优化结果对虚拟学习和实际生产提供了可视化指导。  相似文献   

Effect of soil matric potential on tomato yield and water use under drip irrigation condition     

Dan Wang  Yaohu Kang  Shuqin Wan 《Agricultural Water Management》2007

Field experiment was carried out to investigate the effect of soil matric potential (SMP) on tomato yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) under drip irrigation condition in North China Plain. The experiment included five treatments, which controlled SMP at 0.2 m depth immediately under drip emitter higher than −10 (S1), −20 (S2), −30 (S3), −40 (S4) and −50 kPa (S5), respectively, after tomato plant establishment. The results showed that different SMP affected irrigation amount and tomato ET. Irrigation amount decreased from 185 mm (S1) to 83.6 mm (S5) in 2004, and from 165 mm (S1) to 109 mm (S5) in 2005, respectively. The ET decreased from 270 mm (S1) to 202 mm (S5) in both years. However, it was found that SMP did not affect the tomato yield significantly, for the range of SMP investigated. Both WUE and IWUE increased as SMP decreased. The maximum WUE (253 and 217 kg/ha mm) and IWUE (620 and 406 kg/ha mm) were for S5 in 2 years, whereas the minimum WUE (178 and 155 kg/ha mm) and IWUE 261 and 259 kg/ha mm) were for S1 in 2004 and 2005. Based on the above results, therefore, it is recommended that if the tomatoes are well irrigated (SMP is higher than −20 kPa) during establishment, controlling SMP higher than −50 kPa at 0.2 m depth immediately under drip emitter can be used as an indicator for drip irrigation scheduling during following period of tomato growth in North China Plain.  相似文献