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641.
Differences achieved in 18 selected morphological, phenological and grain parameters attributable to the wheat breeding process were evaluated on the basis of 262 original Czech and Slovak winter bread wheat cultivars (Triticum aestivum L.) developed during 1911–2008 and cultivated in the field and climatic conditions of Crop Research Institute Prague-Ruzyně for 3 years (2010–2012). The wheat breeding process unambiguously has improved grain production parameters, lodging and mildew resistance compared to wheat landraces and obsolete cultivars. Only wheat landrace ‘Visnovska Hustoklasa’ showed a higher resistance to lodging (8 points), no old cultivars registered till 1930 were resistant to mildew. Nevertheless, these economically positive properties of modern wheat cultivars are negatively reflected in lower crude protein content, protein content’s lower stability year to year, and decreased stability of Zeleny sedimentation. The breeding of wheat cultivars during 1961–1990 points to a key period responsible for these parameter changes. At the same time, within this somewhat older wheat group (from 1961–1990), several perspective wheat cultivars with acceptable spike productivity (e.g., ‘Regina’) and high crude protein stability (e.g., ‘Diana II’) were also identified.  相似文献   
642.
Results are reported of the influence of soilless culture upon the biological value of vegetables grown on gravel in glasshouses: 1. Genetic differences of cultivars used in the experiments will also be obvious by means of contents of nutrients by this method of cultivation. 2. Comparing greenhouse crops in soil as usual with those of soilless culture differences as regard the biological value are found sometimes, as follows:
  1. In cucumbers and radishes a slight decrease of dry matter and sugar contents, but no differences in ascorbic acid.
  2. In kohlrabi a slight decrease of all nutrients investigated.
  3. In cauliflower — no differences at all.
  4. In capsicum fruits — higher contents in soiless grown culture, especially of ascorbic acid.
  5. In tomatoes have been detected some features, which are obviously due to cultivation in greenhouses: A decrease of ascorbic acid contents when harvested in summertime and still more distinct when harvested in autumn. Furthermore a decrease of ‘relative’ sugar contents in dry matter in fruits of yield in autumn compared with that of summer yield.
As far as any differences in contents of nutrients occurred they generally were low. As regard the biological value, we may conclude, that soilless grown crops are somewhat equivalent to crops grown in soil. 3. Dates in contents of nutrients found in our investigations of 6 vegetable species are in close relations to corresponding dates published in synoptical tables of nutritive value of vegetables.  相似文献   
643.
Microbial communities were studied in redoximorphic microsites of highly heterogeneous Gleysol at a mm scale using 16S and 18S amplicon sequencing to demonstrate if the composition of soil microbes reflects the differences in ferric and ferrous micro-sites. In both explored gley horizons with redoximorphic features (Bg2 and Cg), ferric mottles were significantly enriched with total P and Fe and depleted of O, Si, Al, K and Ca compared with the adjacent ferrous groundmass (SEM–EDS). Ferric mottles were determined as Fe oxide coatings and hypocoatings. In Bg2, both prokaryotic and micro-eukaryotic communities differed significantly between mottles and groundmass in composition of operational taxonomic units (OTUs) and in proportions of phyla, reflecting heterogeneities in the soil properties there. Mottles in Bg2 were characterized by increased proportion of Proteobacteria, decreased proportion of Acidobacteriota among prokaryotes and by dominance of a single proteobacterial OTU from Anaplasmataceae compared to all other samples. The composition of micro-eukaryotes showed an opposite trend, as micro-eukaryotes of Bg2 groundmass were unique among the other horizons, while micro-eukaryotes of Bg2 mottles had similar composition to neighbouring horizons. Microbial communities of adjacent samples were not more similar to each other than communities of randomly selected ones in Bg2 horizon. That suggests that at mm scale, the sample distance does not represent the driving factor of microbial community composition and that the adjacent samples differ rather due to physicochemical factors. The spatial organization of microbial communities revealed in Bg2 has not reappeared in similarly organized Cg horizon, probably due to other overriding factors. The differences revealed between Bg2 and Cg horizons, including granulometric composition, content of crystalline Fe, exchangeable Al, and organic carbon, as well as exposition to groundwater, were discussed as possible reasons of the distinct organization in Cg. The similarity of pro−/eukaryotic communities of adjacent and non-adjacent couples suggests no distance decay pattern at a mm scale. The agreement between patchiness in soil properties and microbial communities was revealed for the first time and confirms the importance of microscale patterns in soil.  相似文献   
644.
Trichinella spiralis and Trichinella britovi are species that are frequently found in domestic pigs and various sylvatic animals in Croatia. During routine trichinoscopy, non-encapsulated larvae were detected in the muscle tissue of a domestic pig. Artificial digestion revealed a larvae burden of 602 muscle larvae per gram of tissue. Tissue section analysis confirmed the presence of non-encapsulated larvae. Multiplex PCR identified the larvae as T. pseudospiralis. This observation is consistent with the reports of a local veterinary inspector who described the presence of non-encapsulated Trichinella in four individual cases over the last 2 years. This is the first report of T. pseudospiralis in Croatia and one of very few cases of T. pseudospiralis infection described in domestic pigs. The detection of non-encapsulated larvae stresses the need for implementation of artificial digestion instead of trichinoscopy for the detection and identification of Trichinella infections.  相似文献   
645.
A scale to assess the severity of pruritus in dogs was further validated. Comparison of the scale with one containing visible numerical markings demonstrated that owners were heavily influenced by the presence of numbers, resulting in a loss of the scale's ability to generate continuous data. The presence of a traditional visual analogue scale was therefore essential. The scale was tested on 713 owners who presented their dogs for veterinary attention. Pruritus scores in 408 dogs with skin disease covered the full range of possible values (0 to 10). In 305 dogs with no skin disease, 90 owners gave a score greater than zero. Comparison of the scores seen in pruritic dogs, and dogs with no evidence of skin disease, allowed a 'normal range' of 0–1.9 to be established. The scale was able to discriminate between conditions typically regarded as pruritic or non-pruritic. When the scale was assessed for its ability to detect changes in pruritus score following treatment, a median reduction of 4.4 points was observed. The scale was also used to determine what magnitude of response owners would expect following treatment of their pruritic dogs. Only 12% would have been satisfied with a 50% reduction, a figure that is typically quoted as a satisfactory response in clinical trials of anti-pruritic drugs. As a result, alternative methods of assessing clinical trials are proposed. This study has shown the scale to be a valuable tool for clinical assessment of patients, and for monitoring treatment responses in clinical trials.  相似文献   
646.
ObjectivesTo evaluate amino-terminal pro-B type natriuretic peptide (NT-proBNP) concentration in dogs with renal dysfunction and normal cardiac structure and function.AnimalsEight dogs with renal disease, 23 healthy control dogs.MethodsSerum NT-proBNP concentration was measured in healthy dogs and dogs with renal disease using an ELISA validated for use in dogs. Affected dogs were eligible for inclusion if renal dysfunction was diagnosed based on urinalysis and serum chemistry, and if they were free of cardiovascular disease based on physical exam, systolic blood pressure, and echocardiography.ResultsThe geometric mean serum NT-proBNP concentration was significantly higher in dogs with renal disease (617 pmol/L; 95% CI, 260–1467 pmol/L) than in healthy control dogs (261 pmol/L; 95% CI, 225–303 pmol/L; P = 0.0014). There was a modest positive correlation between NT-proBNP and BUN and creatinine. Median NT-proBNP concentration was not significantly different between groups when indexed to BUN (median NT-proBNP:BUN ratio; renal, 14.2, IQR, 3.93–17.7 vs. control, 16.3, IQR, 9.94–21.2; P = 0.29) or creatinine (median NT-proBNP:creatinine ratio; renal, 204, IQR, 72.6–448 vs. control, 227, IQR, 179–308; P = 0.67).ConclusionDogs with renal disease had significantly higher mean serum concentration of NT-proBNP than control dogs. Renal function should be considered when interpreting NT-proBNP results as concentrations may be falsely elevated in dogs with renal dysfunction and normal cardiac function. The effect of renal disease was lessened by indexing NT-proBNP to BUN or creatinine. Future studies in dogs with both renal and heart disease are warranted.  相似文献   
647.
The objective of this study was to investigate the effects of five isonitrogenous (35% crude protein) diets having different energy contents; 10.85, 11.82, 12.73, 13.69, and 15.06 MJ DE/kg, respectively, on the gonad development and weight gain of Sharptooth catfish fingerlings. Energy contents of the experimental diets were increased with the increasing amount of soy-acid oil (0, 4, 8.5, 13, 18%) and each group of fish was fed on their respective diets (group I was fed diet I) during 180 days. The gonad maturation was significantly affected by dietary energy. In both sexes, the histological inspections of the gonads did reveal differences among the fish fed different energetic diets. Fish fed diets I, II, and III had significantly heavier and more developed gonads compared to those on diets IV and V. Fish fed the higher energetic diets (IV and V) had partially low numbers of yolky oocytes compared to other groups. The mean GSI values of female and male fish fed with diet III was significantly greater than those fed diets with higher dietary energy; however, higher HSI values were recorded for fish fed with the higher energetic diets (IV and V). Also, the best weight gain for both males and females were recorded from the fish fed diet III (12.73 MJ DE/kg). Therefore, on the basis of the present results, among energy levels tested, 12.73 MJ DE/kg was found to be optimal for healthy gonad development and weight gain.  相似文献   
648.
Microbiological quality, sensory attributes and consumer preferences of common carp (Cyprinus carpio L.) fillets descaled by four different methods under two storage conditions (air packed and vacuum packaged) and refrigerated storage conditions were studied. Farmed common carps (scaly and mirror), of marketable size (2.2 kg, 3 years old), were randomly divided into four experimental groups (n = 10), and filleted and fillets were processed by four descaling methods: without skin, without scales, undercut scales and mirror carp (without any other treatment). Microbiological analyses were determined by total viable counts (TVCs) in logarithm of colony-forming units per gram (log CFU/g), and sensory quality of fillets was classified by odour, flavour, after taste and consistency on storage days 0, 3, 6 and 9. Vacuum packaging conditions affected the growth of microbiota and extended the shelf life of fillets in all experimental groups. Microbial communities grew to between 3.19–3.90, 4.03–6.00 and 6.35–8.28 log CFU/g after 3, 6 and 9 days of storage, respectively, and fillets without skin showed the lowest TVC among all analysed groups throughout storage. Also sensory analyses showed that consistency and odour of carp fillets were influenced by packaging conditions as well as descaling methods. The survey on consumer preferences of carp found out that 58.9 % of customers preferred scaly carp with undercut scales and processed to fillets instead of mirror carp. The results of present study show that different types of descaling methods combined with air/vacuum packaging can significantly prolong the shelf life of carp fillets.  相似文献   
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