A Cathepsin B Inhibitor,E-64, Improves the Preimplantation Development of Bovine Somatic Cell Nuclear Transfer Embryos     

Sung-Hun MIN  Bong-Seok SONG  Ji-Yeong YEON  Jin-Woo KIM  Jung-Ho BAE  Soo-Yong PARK  Yong-Hee LEE  Kyu-Tae CHANG  Deog-Bon KOO 《The Journal of reproduction and development》2014,60(1):21-27

Bovine somatic cell nuclear transfer (SCNT) is an important and powerful tool for basic research and biomedical and agricultural applications, however, the efficiency of SCNT has remained extremely low. In this study, we investigated the effects of cathepsin B inhibitor (E-64) supplementation of culture medium on in vitro development of bovine SCNT embryos. We initially used three concentrations of E-64 (0.1, 0.5, 1.0 μm), among which 0.5 μm resulted in the highest rate of blastocysts production after in vitro fertilization (IVF), and was therefore used for further experiments. Blastocyst development of SCNT embryos in the E-64 treatment group also increased relative to the control. Moreover, the cryosurvival rates of IVF and SCNT blastocysts were increased in E-64 treatment groups when compared with the control. On the other hand, we found that IVF and SCNT blastocysts derived from E-64-treated groups had increased total cell numbers and decreased apoptotic nuclei. Furthermore, assessment of the expression of apoptosis-related genes (Bax and Bcl-xL) in bovine IVF and SCNT blastocysts treated with E-64 by real-time RT-PCR analysis revealed suppressed expression of the pro-apoptotic gene Bax and stimulated expression of the anti-apoptotic gene Bcl-xL. Taken together, these finding indicate that addition of E-64 to embryo culture medium may have important implications for improving developmental competence and preimplantation quality in bovine IVF and SCNT embryos.  相似文献   

Preliminary Study Characterizing the Use of Sitagliptin for Glycemic Control in Healthy Beagle Dogs with Normal Gluco-Homeostasis     

Hitomi ODA  Akihiro MORI  Peter LEE  Kaori SAEKI  Toshiro ARAI  Toshinori SAKO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1383-1387

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Impact of the 1999 earthquake on mountain stream fishes in Taiwan   总被引：1，自引：0，他引：1  

LEE-SHING  FANG  I-SHIUNG  CHEN  KWEE SIONG  TEW  CHIAO-CHUAN  HAN  TSAI-FU  LEE  I-MING  CHEN 《Fisheries Science》2002,68(2):446-448

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Pathological changes induced by three myxosporeans in the intestine of cultured tiger puffer, Takifugu rubripes (Temminck and Schlegel)     

Tin Tun  K Ogawa  & H Wakabayashi 《Journal of fish diseases》2002,25(2):63-72

Pathological changes in cultured tiger puffer, Takifugu rubripes , with emaciation disease in Kyushu, Japan were studied histologically. In most cases, diseased fish were heavily infected with at least one of three myxosporeans ( Myxidium fugu , Myxidium sp. and Leptotheca fugu ) and two unidentified hyperparasitic microsporeans, attached to, or in, the intestinal epithelium. Myxidium fugu attached to the surface of the epithelium, caused no noticeable effects on the host tissue, irrespective of its infection with the hyperparasite. Myxidium sp., which proliferated in the epithelium, induced severe pathological changes including accumulation of cell debris between the epithelium and lamina propria and resultant detachment of the epithelium. Leptotheca fugu, another histozoic myxosporean, induced degeneration of the epithelium, associated with massive infiltration of macrophages into the epithelium to encapsulate parasites. When L. fugu was infected with its hyperparasitic microsporean, shortened villi were also observed. This is probably because passage of macrophage-parasite aggregates through the basement membrane of the epithelium severely damaged the epithelial structure. It is evident histologically that, unlike epicellular M. fugu , histozoic Myxidium sp. and L. fugu with or without hyperparasitic microsporeans, were highly pathogenic to host fish. This strongly suggests that they are causative agents of the emaciation disease.  相似文献   

降雨特性的模糊理论分析     

李锦育  余新晓 《水土保持研究》2010,17(2)

台湾雨量站的密度高居全世界前3名,然而雨量站往往由于设站时间不一致,或因天灾人祸而导致数据遗失;因此各水文观测站之降雨数据具模糊特性。降雨事件的特性,大致可区分为3个重要因子;降雨量、降雨时间及形状因子。常用之统计值为总降雨量、平均降雨量、最大降雨量、最小降雨量、中值降雨量、降雨延时、尖峰达到时间、偏态指标及峰态指标等9个物理量。研究以统计量及模糊理论为基础,分析屏东县气象局1992-2007年31个雨量站之总降雨量大于100mm,降雨延时3h以上之降雨事件(1177场)。研究结果显示:屏东县大于100mm之降雨,具长延时、高狭峰之特性;就气象站而言,气象站C0R38之暴雨事件,最接近整体降雨特性。  相似文献   

Polycystic kidney disease in a family of Persian cats   总被引：1，自引：0，他引：1  

D S Biller  D J Chew  S P DiBartola 《Journal of the American Veterinary Medical Association》1990,196(8):1288-1290

A 6-year-old Persian cat was determined to have polycystic kidney disease (PKD). Because of 3 previous clinical reports of PKD in Persian cats, the offspring were examined by use of ultrasonography, which provided evidence of PKD in 3 of the 4 offspring. Because of the genetic transmission of this disease, breeders should be advised not to breed PKD-positive Persian cats.  相似文献   

Diaphragmatic rupture and biliary tract damage in a dog     

C. L. DONALD  R. LEE  M. SULLIVAN 《The Journal of small animal practice》1985,26(2):61-66

A 9-month-old bitch was presented with respiratory distress 7 days after a road traffic accident. Radiographs showed a severe hydrothorax and moderate ascites. The removal of bile-stained fluid by thoracentesis reduced the dyspnoea. A diaphragmatic and a hepatic duct rupture were found and repaired at laparotomy. A biliary peritonitis and pleuritis was present. A blood bilirubin of 67 μmol/1 and elevated serum enzymes were present prior to surgery. These changes were largely reversed once the leakage was stopped.  相似文献   

Urinary indices of renal function in sheep with induced aminoglycoside nephrotoxicosis   总被引：1，自引：0，他引：1  

F Garry  D J Chew  G F Hoffsis 《American journal of veterinary research》1990,51(3):420-427

Aminoglycoside nephrotoxicosis (AGNT) was induced in ewes by daily SC administration of gentamicin. Changes in urinary indices of renal function during the development of AGNT are reported. Measurements from timed, volume-measured urine samples were made on days 0, 7, and 8 and included creatinine clearance, total excretion (TE) rates of electrolytes (Na, K, Cl, P) and urine volume. Measurements from free-catch urine samples (without volume measurement) were made daily and included fractional excretion (FE) rate of electrolytes, urine osmolality, and urine-to-serum osmolality and urine-to-serum creatinine ratios. With the onset of AGNT, FE rates of Na, K, Cl, and P- increased many fold above baseline values (200x, 4 to 5x, 6 to 9x, and 70 to 95x, respectively, on days 7 and 8), indicating decreased tubular reabsorption or increased tubular secretion. The increased FE rates were not representative of increases in total electrolyte excretion rates. The total excretion of Na (TENa) was mildly increased, TEK was decreased, TECl was unchanged, and TEP was significantly increased on days 7 and 8. Abnormal urinalysis results, glucosuria, and increased FEP preceded appreciable increase in serum creatinine concentration. Other abnormal urinary indices of renal function coincided with or followed the increase in serum creatinine concentration. Urinary indices may help characterize renal function associated with the disease state, but did not provide early indication of AGNT.  相似文献   

Dicoumarol toxicity in cattle associated with ingestion of silage containing sweet vernal grass (Anthoxanthum odoratum)     

DJ RUNCIMAN  AM LEE  KFM REED  JR WALSH 《Australian veterinary journal》2002,80(1):28-32

A diagnosis of dicoumarol toxicity in a herd of Friesian cattle was made following investigation of the deaths of three mature cows and eleven yearling heifers. Affected stock had been fed wrapped, bailed silage containing approximately 90% sweet vernal grass ( Anthoxanthum odoratum ). Sweet vernal grass contains coumarin, which can be converted to dicoumarol, a vitamin K antagonist, through the action of moulds. Most deaths were preceded by lethargy, severe anaemia and subcutaneous and internal haemorrhage. Dicoumarol toxicosis was suspected based on clinical signs, necropsy findings and prolonged prothrombin and activated partial thromboplastin times. Dicoumarol analysis of blood from affected animals and silage confirmed the diagnosis.  相似文献   

Calcinosis circumscripta and renal dysplasia in a dog     

THELMA LEE GROSS 《Veterinary dermatology》1997,8(1):27-32

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