不同药剂防治玉米草地贪夜蛾试验     

罗文辉  郭瑞光  刘昌敏  曹祥福  刘志强  郭晚心 《长江蔬菜》2021,(2):71-73

以6%阿维·氯苯酰SC(悬浮剂)为对照、清水为空白对照,研究了10%甲维·茚虫威SC、3%甲维盐WG(水分散粒剂)、15%多杀·茚虫威SC、30%氟铃·茚虫威SC、20%四唑虫酰胺SC 5种药剂防治玉米草地贪夜蛾的效果。试验结果表明,6种药剂对玉米草地贪夜蛾均有防治效果,药后3 d,以10%甲维·茚虫威SC、3%甲维盐WG、15%多杀·茚虫威SC防治效果最好,防治效果分别为82.94%、72.31%、65.08%;药后7 d,3%甲维盐WG、10%甲维·茚虫威SC、30%氟铃脲·茚虫威SC、15%多杀·茚虫威SC防治效果分别为91.49%、88.03%、70.25%、66.01%;药后14 d,除本地常用农药6%阿维·氯苯酰SC之外,其他几种农药保叶效果都在70%以上。综上,6种农药均可用于防治玉米草地贪夜蛾,建议轮换使用。  相似文献   

黔东南州打造全域森林公园的可行性研究     

粟燕  刘人源  覃斌  李权 《绿色科技》2020,(4):152-153,157

指出了建设全域森林公园是经济和社会发展的必然要求。在对黔东南州发展全域森林公园进行必要性和打造条件后认为:黔东南州在打造全域森林公园方面具有明显的政策优势,有着优良的森林生态环境资源,以及良好的林业产业基础等优势条件。通过不断完善相关的配套服务体系,已具备了打造全域森林公园的可行性。  相似文献   

京津冀一次区域性暴雨特征分析     

李美琪  贾小卫  郭蕊  吴丹  刘浩 《绿色科技》2020,(6):34-37,42

利用FNL再分析资料、卫星资料和自动站资料对2019年7月22~23日京津冀一次区域性暴雨过程进行了环流形势、卫星云图、动热力结构的分析。结果表明:此次暴雨发生在500hPa两槽一脊、副热带高压北抬、低空急流的大尺度环流背景下。西南方向的水汽输送和黄渤海的补充水汽为暴雨提供了良好的水汽条件,在河北中部的水汽辐合抬升则为暴雨提供了抬升条件。此次暴雨的产生受中尺度对流云团的连续生成合并影响,降水效率高。在暴雨中心整层表现为低层辐合、高层辐散,上升速度中心明显,使得高层的抽吸作用更强,更有利于上升运动的维持与发展,为降水强度增大提供了有力的动力条件,高空弱冷空气的入侵对能量锋区不稳定能量的释放起到了触发作用。  相似文献   

利用GGE双标图分析花生品质性状的基因型-环境互作     

崔顺立  何美敬§  侯名语  杨鑫雷  穆国俊  刘立峰 《中国油料作物学报》2021,43(4):617

花生品质性状的稳定性和基因型-环境互作研究，可为花生品质育种及不同生态区域花生品种的选择提供参考依据。本研究利用GGE-biplot工具，对我国黄淮海花生主产区16个花生品种两年间的品质性状进行了综合分析，包括含油量、油酸、亚油酸、棕榈酸和蛋白质含量等。结果显示，5个品质性状均有较高的GGE总变异值（主成分因素PC1和PC2变异的总和），变幅在61.5%-79.9%之间，以含油量的GGE变异值最低，为61.5%，油酸含量的GGE变异值最高，为79.9%。16个花生品种2年间部分品质性状表现较为一致，其中濮花9519的含油量表型值波动最小，山花9号的亚油酸含量表型值波动最小，开农49是油酸含量表型值最为稳定的品种，天府23号是棕榈酸含量和蛋白质含量表型值最为稳定的品种。初步明确了徐州和濮阳分别适合高油花生和高油酸花生种植，确定了不同生态类型试点下较适合种植的品种，为花生品种推广应用提供了参考。  相似文献   

Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm     

Wang Zhun  Zhang Hengheng  Dong Qiang  Gui Huiping  Wang Xiangru  Pang Nianchang  Li Yongnian  Niu Jing  Jin Dingsha  Wang Sujie  Zhang Xiling  Song Meizhen 《棉花学报》2021,32(6):538-551

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.  相似文献   

粪肠球菌生长曲线的测定及其对小鼠脑组织的影响     

王蒙蒙  张莉  李慧  汪骁轩  魏殿华  高静雯  高建鹏  张华雷  齐亚银 《中国畜牧兽医》2018,45(4):1041-1049

为测定致羔羊脑炎粪肠球菌（Enterococcus faecalis）的生长曲线，寻求一种快速而准确的方法测定不同生长时期粪肠球菌数量，并客观评价其毒性强弱及其对小鼠脑组织的影响，试验采用平板菌落计数法和OD-Monitor振荡比浊法（D_λ值法）测定粪肠球菌的生长曲线，探究该菌在合适时间段内的吸光值（D_{600 nm}）与平板菌落计数法测定的活菌数（CFU）的关系。用粪肠球菌感染小鼠，观察记录小鼠的死亡情况，最后采用Karber法计算粪肠球菌感染小鼠的半数致死量（LD₅₀）。用LD₅₀的剂量感染小鼠，及时采集死亡小鼠脑组织，未死亡的小鼠72 h后全部剖杀取脑组织，一部分做涂片染色，制作病理切片，观察病理变化；一部分进行培养，用于PCR方法进行细菌的回收鉴定。结果显示，用两种方法测定此株粪肠球菌的生长曲线基本一致，在2~8 h生长迅速，为对数生长期，8~14 h生长缓慢，为稳定期，14 h之后死亡数增加，进入衰亡期；对12 h粪肠球菌D_{600 nm}与CFU的关系进行探讨，成功建立回归方程：y=20.769x-1.3422，R²=0.997；其感染小鼠的LD₅₀为7.77×10¹¹个活菌。以此剂量感染小鼠，脑组织涂片染色和培养染色，均能看到革兰氏阳性球菌；PCR结果显示，均出现了大小为112 bp的条带。对脑组织进行病理学观察发现该菌可导致脑组织充血、出血、形成微血栓，脑膜充血。通过生长曲线和其D_{600 nm}与CFU关系的建立，可实时监测粪肠球菌数量，为后期更深入研究粪肠球菌穿越血脑屏障的机制奠定重要的理论基础。  相似文献   

Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation     

Jing Wang  Bi'e Tan  Jianjun Li  Xiangfeng Kong  Minjie Tan  Guoyao Wu 《动物营养（英文）》2020,6(4):438

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.  相似文献   

正交设计优化青蒿SRAP-PCR反应体系及引物筛选     

石紫薇  梁妍  姜婷婷  梁剑平  贾宁  辛志君  陆锡宏  周翔  李雪虎 《分子植物育种》2020,(10):3303-3310

为了建立青蒿的SRAP最佳扩增体系,并筛选出SRAP多态性引物,本研究以青蒿叶片DNA为模板,采用正交试验设计,以Mg^2+、dNTP Mix、Taq DNA聚合酶、引物和DNA模板5种因素5个水平,对青蒿SRAP反应体系进行研究。结果表明,青蒿SRAP-PCR最佳反应体系为:引物0.6μmol/L、Mg^2+2.0 mmol/L、模板DNA 5.1 ng、Taq DNA聚合酶2.0 U、dNTPs 0.25 mmol/L,总体积为25μL。各因素对扩增反应均有不同影响,其中引物浓度的影响最大,dNTPs的影响最小。运用该体系对不同种质资源的青蒿进行验证,证明该体系稳定可靠,并在30个引物组合中筛选出了25对扩增条带清晰,多态性丰富的引物组合。这一结论为今后利用SRAP标记技术进行青蒿分子遗传学研究提供了科学依据。  相似文献   

河北省杂粮生产现状及生产布局展望     

刘猛  刘斐  赵宇  夏雪岩  李顺国 《农学学报》2021,11(3):93-100

河北省是杂粮生产大省,且种类繁多。为稳定河北省杂粮生产发展,进一步调整河北省种植业结构提供参考依据,本文总结了河北省杂粮的历史生产现状,包括面积、单产、总产以及河北省各地市杂粮的分布,试图探索杂粮的生产布局与趋势,同时针对河北省杂粮历史单产水平变化趋势,分析了造成单产变化的成因,并简单提出建议。最后提出河北省杂粮生产布局的展望,将河北省杂粮生产区域布局划分为优势主产区、战略提升区、稳步发展区三个功能区。  相似文献   

New Polyketides from the Antarctic Fungus Pseudogymnoascus sp. HSX2#-11     

Ting Shi  Yan-Yan Yu  Jia-Jia Dai  Yi-Ting Zhang  Wen-Peng Hu  Li Zheng  Da-Yong Shi 《Marine drugs》2021,19(3)

The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi.  相似文献